ISCasp3
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_019752 | ND | Calothrix sp. | Calothrix sp. PCC 6303 plasmid pCAL6303.03 |
DNA section
IS Length : 1734 bp
Ends
IR Length : 18
IRL : GGGTGCGGCTCTTTTAGAAGTACGTTATACTTTGGAAGAATAAAGATGAG
IRR : GGGTGCGGCTCTTTTAGAGAGACAAAGAATGACATTTAGATAGGCATTGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTAAACGTAT | ATATGATTT | AGAGCAAAAA | 9 |
DNA sequence
GGGTGCGGCTCTTTTAGAAGTACGTTATACTTTGGAAGAATAAAGATGAGAAGGCTGTCAGAGTAGTATTTTGAGCAAATAATCAGGTATTGGTGACGGG
CTTTCCAAGCAAGCATCTATTGGGAGATAATTGAGACATATGAAAGCATCTACCTCAAAAGAAGACATGTTTTTCTCGGAAGCACGAGAGCTATTTGAAG
AAATGGTGAACTGGTTAAGTGAGGATAGTGTTTGCGGTTTAGAGCATGGAGAATTAGAGAACAAACTCCTGGATAACGGAAACGAACTATTAAGAAGACT
GTTACAAGGCTATTTAAACAGACGTAGTAATGATGAAATAGAGGGGGAGTGTTTGGGGAGTGACTTAGCTAAAAGAACCCACAAAAAAAGATTTTCCCGG
ACACTGACAACAATATTTGGCACAGTGACAGTCAAGCGAATTGGCTACGGAGGAAGAAAAACCACTTCTCTGAATCCATTGGATGCGGAATTAAACCTAC
CAGAATCTAAGTATTCCCACGGAATTAGGCAACGAATAGCAGTGGAGGCGGCACGTTCGGGTTTTGGCGAGACAACAGAAATAATCAAGAAAGCGATGCC
AGTTGAGATAGGTAAGCGTCAAGTAGAAGACTTGGCTCGCCATAGTGCTTGCGATTTTGATGATTTCTATAGCGAACAACAAGCACAGTCAAGGGAACTG
GAGGAAACCGGAGAAATTGTGGTAATCAGTGCAGACGGAAAGGGTGTTGTTATGCGTACAGAAGATTTACGTGCTGAGACTCAAAAAAGGGCGCTTGCTT
CAAACAAAAAGTTAAATAAACGATTGACCAAGGGAGAAAAACGTAACTCAAAGCGAATGGCTACGGTGGCTTCAGTTTATACTATTAACCCATTTGTCCG
TACTGCTCAACAAATCGTAAACCCAGAAGAAGAAGATAAAAAAAATCTCAAACGACCTCGACCACAAGGTAAGCGTGTTTGGGCAAGCCTAGTTAAAGAG
CCTGAACAAGTCCTAAAAGAGGCATTTGATGAAGCATTACACCGCGACCCCAATCACCAAAAGCACTTTTGTGCCCTAGTAGATGGTAATAAGACACAAT
TGTCACTGTTGAATAAATTCGCTCGTCAACACAATCTCAAGTTAACCATCGTCCTAGACATTATTCATGTGATTGAGTATTTGTGGAAAGCTGCGTTTGC
CTTCTATGAGAATACCAGTAAAGAGGCAGAAGCTTGGGTAAGCAAACGTTTGCTGCTTATCCTAGAAGGCAAGTCGAGCCAGGTTGCTGCTGGTATGCGT
CGCAGCGCCACTCTACGCAAACTTACTCCATCAGAACGAAAATCAGTCGATGCCTGTGCCAGATATTTGCTTAATAACAGTGCTTACCTTAAATACGATG
ATTACTTGAAAGCTGGGCTGCCGATTGCGACTGGAGTCATTGAGGGGGCTTGTCGTCACCTCATTAAAGATCGAATGGATATTACAGGTGCTCGCTGGAG
CCTTGAAGGTGCGGAAGCTGTTTTACGACTTCGTTCCCTTTACATTAGTGGTGATTGGGATGAGTATTGGCGCTTCCATTTAAAACAGGAGCATCAACGC
AATCACTCTTGCTTCTATAAAGACGGCATTCCTTTAATGAAGCGAGTAATTCAAGCTCGCTGTTCTACTACTGCTCCCACGGTCGCAATGCCTATCTAAA
TGTCATTCTTTGTCTCTCTAAAAGAGCCGCACCC
CTTTCCAAGCAAGCATCTATTGGGAGATAATTGAGACATATGAAAGCATCTACCTCAAAAGAAGACATGTTTTTCTCGGAAGCACGAGAGCTATTTGAAG
AAATGGTGAACTGGTTAAGTGAGGATAGTGTTTGCGGTTTAGAGCATGGAGAATTAGAGAACAAACTCCTGGATAACGGAAACGAACTATTAAGAAGACT
GTTACAAGGCTATTTAAACAGACGTAGTAATGATGAAATAGAGGGGGAGTGTTTGGGGAGTGACTTAGCTAAAAGAACCCACAAAAAAAGATTTTCCCGG
ACACTGACAACAATATTTGGCACAGTGACAGTCAAGCGAATTGGCTACGGAGGAAGAAAAACCACTTCTCTGAATCCATTGGATGCGGAATTAAACCTAC
CAGAATCTAAGTATTCCCACGGAATTAGGCAACGAATAGCAGTGGAGGCGGCACGTTCGGGTTTTGGCGAGACAACAGAAATAATCAAGAAAGCGATGCC
AGTTGAGATAGGTAAGCGTCAAGTAGAAGACTTGGCTCGCCATAGTGCTTGCGATTTTGATGATTTCTATAGCGAACAACAAGCACAGTCAAGGGAACTG
GAGGAAACCGGAGAAATTGTGGTAATCAGTGCAGACGGAAAGGGTGTTGTTATGCGTACAGAAGATTTACGTGCTGAGACTCAAAAAAGGGCGCTTGCTT
CAAACAAAAAGTTAAATAAACGATTGACCAAGGGAGAAAAACGTAACTCAAAGCGAATGGCTACGGTGGCTTCAGTTTATACTATTAACCCATTTGTCCG
TACTGCTCAACAAATCGTAAACCCAGAAGAAGAAGATAAAAAAAATCTCAAACGACCTCGACCACAAGGTAAGCGTGTTTGGGCAAGCCTAGTTAAAGAG
CCTGAACAAGTCCTAAAAGAGGCATTTGATGAAGCATTACACCGCGACCCCAATCACCAAAAGCACTTTTGTGCCCTAGTAGATGGTAATAAGACACAAT
TGTCACTGTTGAATAAATTCGCTCGTCAACACAATCTCAAGTTAACCATCGTCCTAGACATTATTCATGTGATTGAGTATTTGTGGAAAGCTGCGTTTGC
CTTCTATGAGAATACCAGTAAAGAGGCAGAAGCTTGGGTAAGCAAACGTTTGCTGCTTATCCTAGAAGGCAAGTCGAGCCAGGTTGCTGCTGGTATGCGT
CGCAGCGCCACTCTACGCAAACTTACTCCATCAGAACGAAAATCAGTCGATGCCTGTGCCAGATATTTGCTTAATAACAGTGCTTACCTTAAATACGATG
ATTACTTGAAAGCTGGGCTGCCGATTGCGACTGGAGTCATTGAGGGGGCTTGTCGTCACCTCATTAAAGATCGAATGGATATTACAGGTGCTCGCTGGAG
CCTTGAAGGTGCGGAAGCTGTTTTACGACTTCGTTCCCTTTACATTAGTGGTGATTGGGATGAGTATTGGCGCTTCCATTTAAAACAGGAGCATCAACGC
AATCACTCTTGCTTCTATAAAGACGGCATTCCTTTAATGAAGCGAGTAATTCAAGCTCGCTGTTCTACTACTGCTCCCACGGTCGCAATGCCTATCTAAA
TGTCATTCTTTGTCTCTCTAAAAGAGCCGCACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1560 bp | 519 aa | 140 | 1699 | + | No |
Chemistry : DDE
ORF sequence :
MKASTSKEDMFFSEARELFEEMVNWLSEDSVCGLEHGELENKLLDNGNELLRRLLQGYLNRRSNDEIEGECLGSDLAKRTHKKRFSRTLTTIFGTVTVKR
IGYGGRKTTSLNPLDAELNLPESKYSHGIRQRIAVEAARSGFGETTEIIKKAMPVEIGKRQVEDLARHSACDFDDFYSEQQAQSRELEETGEIVVISADG
KGVVMRTEDLRAETQKRALASNKKLNKRLTKGEKRNSKRMATVASVYTINPFVRTAQQIVNPEEEDKKNLKRPRPQGKRVWASLVKEPEQVLKEAFDEAL
HRDPNHQKHFCALVDGNKTQLSLLNKFARQHNLKLTIVLDIIHVIEYLWKAAFAFYENTSKEAEAWVSKRLLLILEGKSSQVAAGMRRSATLRKLTPSER
KSVDACARYLLNNSAYLKYDDYLKAGLPIATGVIEGACRHLIKDRMDITGARWSLEGAEAVLRLRSLYISGDWDEYWRFHLKQEHQRNHSCFYKDGIPLM
KRVIQARCSTTAPTVAMPI
IGYGGRKTTSLNPLDAELNLPESKYSHGIRQRIAVEAARSGFGETTEIIKKAMPVEIGKRQVEDLARHSACDFDDFYSEQQAQSRELEETGEIVVISADG
KGVVMRTEDLRAETQKRALASNKKLNKRLTKGEKRNSKRMATVASVYTINPFVRTAQQIVNPEEEDKKNLKRPRPQGKRVWASLVKEPEQVLKEAFDEAL
HRDPNHQKHFCALVDGNKTQLSLLNKFARQHNLKLTIVLDIIHVIEYLWKAAFAFYENTSKEAEAWVSKRLLLILEGKSSQVAAGMRRSATLRKLTPSER
KSVDACARYLLNNSAYLKYDDYLKAGLPIATGVIEGACRHLIKDRMDITGARWSLEGAEAVLRLRSLYISGDWDEYWRFHLKQEHQRNHSCFYKDGIPLM
KRVIQARCSTTAPTVAMPI
Blast result :
Comments
ISCasp3 is 81% aa similar to ISCasp2.
References
1] ISfinder annotation (2013)
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T. and Kerfeld,C.A. (2012) Direct submission GenBank.
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T. and Kerfeld,C.A. (2012) Direct submission GenBank.