ISLesp4
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_JH993793 | ND | Leptolyngbya sp. | Leptolyngbya sp. PCC 7375 |
DNA section
IS Length : 1696 bp
Ends
IR Length : 19/28
IRL : GGGTGTGATTCTTTTAGGGTAAAGGTTTGCAACGCAGATTAAGTCTATTG
IRR : GGGTGCGGCTCTTTCTCTGTAAAGCATTAAGCAGTCAAGAATCTCTCCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGATATTCA | ATCTCCATT | GGTGAAAGCG | 9 |
| CATGATATTCA | ATCTCCATT | GGTGAAAGCGA | 9 |
| ACATGGGAGTT | AACTGATAT | CACAAGATCCT | 9 |
| CAGGCAGCATG | TAATGAATT | TCGGGGTCGCA | 9 |
| TGATTTATGCC | AATGGAGCT | AGCAATATCCG | 9 |
| GATTAGCTGCC | ATTCACGAT | TGCGAAGGGTG | 9 |
| ACTGCTTCTAA | AA/TCTGCAAC | CCTGTCTTCTA | 11 |
| ATTCCCAATGGTTTGATGAT | TGCTGATTTCCCAGGCAGTA | 0 | |
| CCGGATAATCC | AACTTAATT | AGTCGTTTGTA | 9 |
| TATTTTGCTCT | ATCTGCGAG | ACTGGCGCGAT | 9 |
| GCTTTCTAAGT | TTCTAAGTT | TACCCAGCTAG | 9 |
| AATTCCAGCGG | AATTGTTTT | TATCAGTCCAG | 9 |
| GAAACCAAGGA | ATCGGAAAA | GCTTTCACGCA | 9 |
| TCTTGATCGGC | ATAGGCATT | TCTGCTGTGAG | 9 |
| TGGGTTTTCTA | TTCGGGATT | GCCGATAATCG | 9 |
| TCTCGACAAAG | AAACTATTA | AGATGATTGAA | 9 |
| CCAGTACCCTC | AAAGCCTAT | GAACGGCAGCT | 9 |
DNA sequence
GGGTGTGATTCTTTTAGGGTAAAGGTTTGCAACGCAGATTAAGTCTATTGAATAGAATTTGGTAGATTTTAGGGGTCTGAAAGTTCAATCTATCTCTTTT
TTGAGAGATAGATTCGACGAAAAAAATAATTGTCTCGTGTGCCTGTATCCTCTCCATTCGAATCATGCCAACAGACCTAAGTCTTAGCTTTTTTGAGTCA
GCGTTAGCGCAGTTTAATGAACTCGTCGATAGGTTGAGTTCAGCTGATAGCCAAAATTGGGAGCATGGAAAACTAGAACGTTATATCCATGAGTCGGGCA
CAGAACTTTTGCGACGGTTATATCAAGGCCATCTCGACTTGCGTTACGCATCAGAGGACTACCAAAGCGAGGTCGTCGGTAGCGATGGAGAAACCCGCCC
CCATCGTCGAAAACAGACCCAACGGAACCTCGAAACGCTGTTTGGGGAAGTGACCTTAAACCGAGTGGGATACAGCACCCAAAAACCTGAGGTCAGTGCC
CTGTACCCGTCAGATGGGATCTTGAATTTGCCGCCGGACAAATACTCGGATGAACTCCGAAAACGAGTCGCGGTGGAAGCGTCGAAAGTATCGTTTTCAG
AAACGAGTCGGACCATAAGCTCGACGACAGGGGGGGCGATTGGTAAACGCCAATGTGAAGAGGTGACGGTAAAAGTGGCCCAAGACTTTGAGGACTTTTA
TGCTCAACGGGCGATAGACCGACGAGTTGCCAGTGATGAGCTATTAGTGATTACCACTGATGGTAAGGGGATTGTGATGCATCCATCAGATCTGCGAGAA
GCCACGGCCAAAGCCGCTCAAAAGGCCAAGGACTCACCGTCAAAACGTACTCGCTTAAGTCCCGGTCAAAAGCGTCAGCGTAAACGAATGGCCACAGTCG
CATCGGTGTATCATGTGCCTCGATACCCCCGTCAAGCCGACGATATCATCGGTGACCAACGGGACCCGCCCGTGCGACCAGTGATTAGTGACAAACGAGT
GTGGGCCAGTGTTGAGCAAGACGCTAAAACGGTCATTGAACATGCGTTTGTCGAAGCCTCGACTCGAGACCCGGACCATCAACGCGACTGGGTTGTCCTG
GTAGATGGTGAACTCCATCAATTAGCCACACTCAACGCCATGGCCCGAAAGCAAGACGTGACGGTGACTATCGTGCTCGATTTCATTCATGTTCTCGAAT
ATGTTTGGAAAGCGGCTTTTTGTTTCTTCTCATCTGGTAGTGAGGAGGCCGAGACTTGGGTCCAAGAGAGAGCCTTACGCATTCTACAAGGTAAAGCCAG
TGATGTGGCGGCCGGTATTCGGCGTAGCGCCACCCTACAAAATCTAGACACAAAAGCACGAGAGAACGTTGACAAGTGCGCTGATTATTTGTTGAAAAAT
CGGCCGTATTTGTTGTATGACGAGTACTTAGATCGGGGGTATCCCATTGCCTCAGGTGTGATTGAAGGCGCGTGCAGGCATTTAGTCAATGACCGCATGG
AGATCACCGGTGCCCGCTGGCGCTTAGATCGTGCTGAAGCCGTTCTGCGGATTCGCGCACTCAGAGCCAGTGATGATTTCGAGGCCTATTGGGAGTTTCA
CAAAGTCCAAGAGTTCAAACGCAATCATGCCAATAAGTTTCTCGAACCGGAGAGATTCTTGACTGCTTAATGCTTTACAGAGAAAGAGCCGCACCC
TTGAGAGATAGATTCGACGAAAAAAATAATTGTCTCGTGTGCCTGTATCCTCTCCATTCGAATCATGCCAACAGACCTAAGTCTTAGCTTTTTTGAGTCA
GCGTTAGCGCAGTTTAATGAACTCGTCGATAGGTTGAGTTCAGCTGATAGCCAAAATTGGGAGCATGGAAAACTAGAACGTTATATCCATGAGTCGGGCA
CAGAACTTTTGCGACGGTTATATCAAGGCCATCTCGACTTGCGTTACGCATCAGAGGACTACCAAAGCGAGGTCGTCGGTAGCGATGGAGAAACCCGCCC
CCATCGTCGAAAACAGACCCAACGGAACCTCGAAACGCTGTTTGGGGAAGTGACCTTAAACCGAGTGGGATACAGCACCCAAAAACCTGAGGTCAGTGCC
CTGTACCCGTCAGATGGGATCTTGAATTTGCCGCCGGACAAATACTCGGATGAACTCCGAAAACGAGTCGCGGTGGAAGCGTCGAAAGTATCGTTTTCAG
AAACGAGTCGGACCATAAGCTCGACGACAGGGGGGGCGATTGGTAAACGCCAATGTGAAGAGGTGACGGTAAAAGTGGCCCAAGACTTTGAGGACTTTTA
TGCTCAACGGGCGATAGACCGACGAGTTGCCAGTGATGAGCTATTAGTGATTACCACTGATGGTAAGGGGATTGTGATGCATCCATCAGATCTGCGAGAA
GCCACGGCCAAAGCCGCTCAAAAGGCCAAGGACTCACCGTCAAAACGTACTCGCTTAAGTCCCGGTCAAAAGCGTCAGCGTAAACGAATGGCCACAGTCG
CATCGGTGTATCATGTGCCTCGATACCCCCGTCAAGCCGACGATATCATCGGTGACCAACGGGACCCGCCCGTGCGACCAGTGATTAGTGACAAACGAGT
GTGGGCCAGTGTTGAGCAAGACGCTAAAACGGTCATTGAACATGCGTTTGTCGAAGCCTCGACTCGAGACCCGGACCATCAACGCGACTGGGTTGTCCTG
GTAGATGGTGAACTCCATCAATTAGCCACACTCAACGCCATGGCCCGAAAGCAAGACGTGACGGTGACTATCGTGCTCGATTTCATTCATGTTCTCGAAT
ATGTTTGGAAAGCGGCTTTTTGTTTCTTCTCATCTGGTAGTGAGGAGGCCGAGACTTGGGTCCAAGAGAGAGCCTTACGCATTCTACAAGGTAAAGCCAG
TGATGTGGCGGCCGGTATTCGGCGTAGCGCCACCCTACAAAATCTAGACACAAAAGCACGAGAGAACGTTGACAAGTGCGCTGATTATTTGTTGAAAAAT
CGGCCGTATTTGTTGTATGACGAGTACTTAGATCGGGGGTATCCCATTGCCTCAGGTGTGATTGAAGGCGCGTGCAGGCATTTAGTCAATGACCGCATGG
AGATCACCGGTGCCCGCTGGCGCTTAGATCGTGCTGAAGCCGTTCTGCGGATTCGCGCACTCAGAGCCAGTGATGATTTCGAGGCCTATTGGGAGTTTCA
CAAAGTCCAAGAGTTCAAACGCAATCATGCCAATAAGTTTCTCGAACCGGAGAGATTCTTGACTGCTTAATGCTTTACAGAGAAAGAGCCGCACCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1506 bp | 501 aa | 165 | 1670 | + | No |
Chemistry : DDE
ORF sequence :
MPTDLSLSFFESALAQFNELVDRLSSADSQNWEHGKLERYIHESGTELLRRLYQGHLDLRYASEDYQSEVVGSDGETRPHRRKQTQRNLETLFGEVTLNR
VGYSTQKPEVSALYPSDGILNLPPDKYSDELRKRVAVEASKVSFSETSRTISSTTGGAIGKRQCEEVTVKVAQDFEDFYAQRAIDRRVASDELLVITTDG
KGIVMHPSDLREATAKAAQKAKDSPSKRTRLSPGQKRQRKRMATVASVYHVPRYPRQADDIIGDQRDPPVRPVISDKRVWASVEQDAKTVIEHAFVEAST
RDPDHQRDWVVLVDGELHQLATLNAMARKQDVTVTIVLDFIHVLEYVWKAAFCFFSSGSEEAETWVQERALRILQGKASDVAAGIRRSATLQNLDTKARE
NVDKCADYLLKNRPYLLYDEYLDRGYPIASGVIEGACRHLVNDRMEITGARWRLDRAEAVLRIRALRASDDFEAYWEFHKVQEFKRNHANKFLEPERFLT
A
VGYSTQKPEVSALYPSDGILNLPPDKYSDELRKRVAVEASKVSFSETSRTISSTTGGAIGKRQCEEVTVKVAQDFEDFYAQRAIDRRVASDELLVITTDG
KGIVMHPSDLREATAKAAQKAKDSPSKRTRLSPGQKRQRKRMATVASVYHVPRYPRQADDIIGDQRDPPVRPVISDKRVWASVEQDAKTVIEHAFVEAST
RDPDHQRDWVVLVDGELHQLATLNAMARKQDVTVTIVLDFIHVLEYVWKAAFCFFSSGSEEAETWVQERALRILQGKASDVAAGIRRSATLQNLDTKARE
NVDKCADYLLKNRPYLLYDEYLDRGYPIASGVIEGACRHLVNDRMEITGARWRLDRAEAVLRIRALRASDDFEAYWEFHKVQEFKRNHANKFLEPERFLT
A
Blast result :
Comments
ISLesp4 is 62% aa similar to ISCasp2.
References
1] ISfinder annotation (2013)
2] Kerfeld,C.A., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J. and Klenk,H.-P. (2012) Direct submission GenBank.
2] Kerfeld,C.A., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J. and Klenk,H.-P. (2012) Direct submission GenBank.