ISTvi1
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_018012 | ND | Thiocystis violascens | Thiocystis violascens DSM 198 |
DNA section
IS Length : 1417 bp
Ends
IR Length : 22
IRL : GGGTGCGACCAGAAGTGATGCGTCGGCTAAACTGTCGATCCCATTTGACT
IRR : GGGTGCGACCAGAAGTGATGCGGGCTGCTCCGCTGTCATGCGGCTTGCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAAGCATTA | GCCAATCGG | GCTGCAATTT | 9 |
DNA sequence
GGGTGCGACCAGAAGTGATGCGTCGGCTAAACTGTCGATCCCATTTGACTTGCTTGAGGCTCCAGCCATGCCAACGCTTGCTGAGCGCGATGAGGAGTTG
ATCCACGCCCTGAACCGTCACCCGCCACTGAAGCAGCGAATTGAGCAGCTCGTGGCGGTGGTGGAAGACGCAGCAGGTGACTTGGCACGCGCCGATGCAG
CGGAACGGCGGGTGATCGAGGAAATCCGCCGCCTTGGCCAGGAGCGCCTTCAAGCCTGGGCGCAGCGGCAGGTGGAACAGACCGCGCGGTCGGTGTTGGA
GCGCGGTGAGGTGCGTTGCGGGGGTAAAAAAACTGTGCTGGCATAGTACGTTCGGATTGATTGAGGTCGAGGAACCGCTGCTTCGTCAGGGGACGCAGGT
GCAGCGACCTTTTTCGGCGCGTGCGCAGGTGACGCATCGGTGTTGCTCACAACCGCTGCAACGGGTGGTGACGGATGTCGGTGCCGATGTCTCGTTTCAG
CAAGCCGCTGGCAAATTATTCGAACACTATGCGGTGGCTTTGCCGGTGGAGACGATCCGTCAAATCGTGGAGGGTCATGCGCAGGCGATCTTTGACGCAC
ACGAAACGGAAGAAGATTGGCCAACCGAACCGGGAACCCCATGGCTGGTTGCGGAACTCGATGGCGGGATGGTCCCGATCATGACCCCGGATCCCGCGCA
GCCCGACCAGCGCCGCGGGAAGAGCTTGGCGTGGAAAGAAGCCAAATTGTGCCTGGTGCATGCCTTGGGCAGTGCGACGCCTTGTTATGGTGGAACCCTT
CAAGGGGGTGTCGAGGAGGTGGGACGCGAACTCTTTAACTGTGCCCGAGAGGCCGGTTTCGGCACCCACACGCGCGTGCACGCCGTGGGCGACGGCGCGC
TGTGGATCGCCGATCAAATCGACCAGCAATTCGGTCAGCAAGGCCAGTTTTTGGTCGATTTCGACCATGCCTGTGAGTATTTGGGCGCCGCCGCCAAGAC
CTGCTCCCCCGACCCAACGGTCTGGCTGGACCGCCAACAACAGCGGCTCAAGACCCATCAACTCGACGCGGTCTTGGCGGAGCTGGCGCACCATCTGGAG
CCAGAGACTGTGGCCGATCCCGACGCCCCGGTGCGGGCCGCACAGCGCTACTTCAGCAACCGCCGTGAGCAATTCGACTACCCGGGCGCGCAGGAACACG
GATTACCCATTGGCTCCGGCGAGATCGAGAGTGCGCATCGGTACGTGGTTCAACAACGACTGAAGCGTCCCGGTGCTTGGTGGTGCGTCGCACAGGCCGA
GGCCATGCTGGCTCTCCGCCTGAACCGGCTCAATGGCCACTGGGAGGGATACTGGCAAGACGTGATCAAGCAAGCCGCATGACAGCGGAGCAGCCCGCAT
CACTTCTGGTCGCACCC
ATCCACGCCCTGAACCGTCACCCGCCACTGAAGCAGCGAATTGAGCAGCTCGTGGCGGTGGTGGAAGACGCAGCAGGTGACTTGGCACGCGCCGATGCAG
CGGAACGGCGGGTGATCGAGGAAATCCGCCGCCTTGGCCAGGAGCGCCTTCAAGCCTGGGCGCAGCGGCAGGTGGAACAGACCGCGCGGTCGGTGTTGGA
GCGCGGTGAGGTGCGTTGCGGGGGTAAAAAAACTGTGCTGGCATAGTACGTTCGGATTGATTGAGGTCGAGGAACCGCTGCTTCGTCAGGGGACGCAGGT
GCAGCGACCTTTTTCGGCGCGTGCGCAGGTGACGCATCGGTGTTGCTCACAACCGCTGCAACGGGTGGTGACGGATGTCGGTGCCGATGTCTCGTTTCAG
CAAGCCGCTGGCAAATTATTCGAACACTATGCGGTGGCTTTGCCGGTGGAGACGATCCGTCAAATCGTGGAGGGTCATGCGCAGGCGATCTTTGACGCAC
ACGAAACGGAAGAAGATTGGCCAACCGAACCGGGAACCCCATGGCTGGTTGCGGAACTCGATGGCGGGATGGTCCCGATCATGACCCCGGATCCCGCGCA
GCCCGACCAGCGCCGCGGGAAGAGCTTGGCGTGGAAAGAAGCCAAATTGTGCCTGGTGCATGCCTTGGGCAGTGCGACGCCTTGTTATGGTGGAACCCTT
CAAGGGGGTGTCGAGGAGGTGGGACGCGAACTCTTTAACTGTGCCCGAGAGGCCGGTTTCGGCACCCACACGCGCGTGCACGCCGTGGGCGACGGCGCGC
TGTGGATCGCCGATCAAATCGACCAGCAATTCGGTCAGCAAGGCCAGTTTTTGGTCGATTTCGACCATGCCTGTGAGTATTTGGGCGCCGCCGCCAAGAC
CTGCTCCCCCGACCCAACGGTCTGGCTGGACCGCCAACAACAGCGGCTCAAGACCCATCAACTCGACGCGGTCTTGGCGGAGCTGGCGCACCATCTGGAG
CCAGAGACTGTGGCCGATCCCGACGCCCCGGTGCGGGCCGCACAGCGCTACTTCAGCAACCGCCGTGAGCAATTCGACTACCCGGGCGCGCAGGAACACG
GATTACCCATTGGCTCCGGCGAGATCGAGAGTGCGCATCGGTACGTGGTTCAACAACGACTGAAGCGTCCCGGTGCTTGGTGGTGCGTCGCACAGGCCGA
GGCCATGCTGGCTCTCCGCCTGAACCGGCTCAATGGCCACTGGGAGGGATACTGGCAAGACGTGATCAAGCAAGCCGCATGACAGCGGAGCAGCCCGCAT
CACTTCTGGTCGCACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 279 bp | 92 aa | 68 | 346 | + | No |
Description : First part of the transposase
ORF sequence :
MPTLAERDEELIHALNRHPPLKQRIEQLVAVVEDAAGDLARADAAERRVIEEIRRLGQERLQAWAQRQVEQTARSVLERGEVRCGGKKTVLA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1167 bp | 388 aa | 216 | 1382 | + | No |
Description : Second part of the transposase
ORF sequence :
SRKSAALARSAFKPGRSGRWNRPRGRCWSAVRCVAGVKKLCWHSTFGLIEVEEPLLRQGTQVQRPFSARAQVTHRCCSQPLQRVVTDVGADVSFQQAAGK
LFEHYAVALPVETIRQIVEGHAQAIFDAHETEEDWPTEPGTPWLVAELDGGMVPIMTPDPAQPDQRRGKSLAWKEAKLCLVHALGSATPCYGGTLQGGVE
EVGRELFNCAREAGFGTHTRVHAVGDGALWIADQIDQQFGQQGQFLVDFDHACEYLGAAAKTCSPDPTVWLDRQQQRLKTHQLDAVLAELAHHLEPETVA
DPDAPVRAAQRYFSNRREQFDYPGAQEHGLPIGSGEIESAHRYVVQQRLKRPGAWWCVAQAEAMLALRLNRLNGHWEGYWQDVIKQAA
LFEHYAVALPVETIRQIVEGHAQAIFDAHETEEDWPTEPGTPWLVAELDGGMVPIMTPDPAQPDQRRGKSLAWKEAKLCLVHALGSATPCYGGTLQGGVE
EVGRELFNCAREAGFGTHTRVHAVGDGALWIADQIDQQFGQQGQFLVDFDHACEYLGAAAKTCSPDPTVWLDRQQQRLKTHQLDAVLAELAHHLEPETVA
DPDAPVRAAQRYFSNRREQFDYPGAQEHGLPIGSGEIESAHRYVVQQRLKRPGAWWCVAQAEAMLALRLNRLNGHWEGYWQDVIKQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1315 bp | 437 aa | 68 | 1382 | + | Yes |
Chemistry : DDE
ORF sequence :
MPTLAERDEELIHALNRHPPLKQRIEQLVAVVEDAAGDLARADAAERRVIEEIRRLGQERLQAWAQRQVEQTARSVLERGEVRCGGKKLCWHSTFGLIEV
EEPLLRQGTQVQRPFSARAQVTHRCCSQPLQRVVTDVGADVSFQQAAGKLFEHYAVALPVETIRQIVEGHAQAIFDAHETEEDWPTEPGTPWLVAELDGG
MVPIMTPDPAQPDQRRGKSLAWKEAKLCLVHALGSATPCYGGTLQGGVEEVGRELFNCAREAGFGTHTRVHAVGDGALWIADQIDQQFGQQGQFLVDFDH
ACEYLGAAAKTCSPDPTVWLDRQQQRLKTHQLDAVLAELAHHLEPETVADPDAPVRAAQRYFSNRREQFDYPGAQEHGLPIGSGEIESAHRYVVQQRLKR
PGAWWCVAQAEAMLALRLNRLNGHWEGYWQDVIKQAA
EEPLLRQGTQVQRPFSARAQVTHRCCSQPLQRVVTDVGADVSFQQAAGKLFEHYAVALPVETIRQIVEGHAQAIFDAHETEEDWPTEPGTPWLVAELDGG
MVPIMTPDPAQPDQRRGKSLAWKEAKLCLVHALGSATPCYGGTLQGGVEEVGRELFNCAREAGFGTHTRVHAVGDGALWIADQIDQQFGQQGQFLVDFDH
ACEYLGAAAKTCSPDPTVWLDRQQQRLKTHQLDAVLAELAHHLEPETVADPDAPVRAAQRYFSNRREQFDYPGAQEHGLPIGSGEIESAHRYVVQQRLKR
PGAWWCVAQAEAMLALRLNRLNGHWEGYWQDVIKQAA
Blast result :
Comments
ISTvi1 is 42% aa similar to ISDere1.
The third ORF is the putative oRFAB transposase recnstructed in silico by possible -1 frmaeshift.
The third ORF is the putative oRFAB transposase recnstructed in silico by possible -1 frmaeshift.
References
1] ISfinder annotation (2013)
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Vogl,K., Liu,Z., Frigaard,N.-U., Bryant,D. and Woyke,T. (2012) Direct submission GenBank.
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Vogl,K., Liu,Z., Frigaard,N.-U., Bryant,D. and Woyke,T. (2012) Direct submission GenBank.