ISLesp2
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_JH993795 | ND | Leptolyngbya sp. | Leptolyngbya sp. PCC 7375 |
DNA section
IS Length : 1502 bp
Ends
IR Length : 20/23
IRL : GGGCGCATCTAAAGGTTCGGGGAACCGTTCAAGCTGATTTGCGAAAATAG
IRR : GGGCGCATCTAGCGGTTAGGGGAGATAATAGGCAATACCAAAGAGTGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGCGAAGTG | GGGGAGTGA | TGGCATTGTG | 9 |
DNA sequence
GGGCGCATCTAAAGGTTCGGGGAACCGTTCAAGCTGATTTGCGAAAATAGAAGGGAAGAAAAAAGGCTATCCGTTGTCCGGCTAGCCCTAATCAAAATCA
CTTCTATGATAACAACCCCTACGAATAGCTATTCTGAATCAGAAGACAGGTCATCCCGACTTGACCGCCTTCATCAAAGTCAAACCTTAGCGGCTATTGT
GATTGCTGCTTGGCAAATAGGGTTACACGTCGCACGCACGCTCGTCGAACAACAGCTCAATGAGCGTGCTAAACGCCCGACCGATTGGGGGCCTTGTTCA
GTGTGCGGCCAGCGGCTACACAGTAAGGGTTTTGCGACTCGTCAGATGGTGACCTTGGTCGGTCTTGTGCAGTGGCGACGCCGATTAGGTCGCTGCCCTC
AAGGGTGCAAAGGGAGCCACGCGGCTCCCTTTGACCAAACGCTGGGGATTGCCCCGTACCAACAAAGTTCTGTAGAGCTAAAGCGATTAGGTTGCCTGCT
GAGCCTCTTCGTTCCGTTCGAGTTAGCCGCTTGGCTCCTGTCTCAATTGAGTGGCATTCAGGTCAGTGATGACAGCCTGTGGCAGTGGGTCCAACAGTAT
GGTCAACAAGCCGCCTGTCAGTGGGAAACAGATGTTGAGAACTGGGAAAACGGAGAAGACCCGGTGCCCGAAGCACTTTCTGACGACCTCGCGGCAATGC
CGTTGTTGATCGCTGCAGATGGCGTCACGGTCCCACTACGTCCACAGGTCGGCACCCCAGCTGGCAAAACGCTTTGGCGAGAAGTCAAAGTCGGCTTACT
GGCTCGACTCAGTCATCGTATTAATCGCCATGGCAAACTTGTTACTCGCCTACAGCGACGACGTTTGGTCGCCGTGCTAGGCGATATGGCTACGTTTCGG
CCTCGCTTGCAGCTCGAAGCAGCGCGAGCGGGCATCGCAACAGCTCCACACGTTGTCTGGCTCAGCGATGGAGCCAGGGGGTTTTGGCGACTCTTCGAGA
CTTGTTTTTCTCACCTAGCGGTCGGCATCCTCGATTTCTATCATGCAGCGGCCTATCTGTGGCAAGCCGCTGATGCCTACAAAGACGGGAACCCGGCACG
CACACCACAGATGTGGTTTGAGCGATTGCGCCACCAGCTCCGACACGGCTACGTCCACCGGATTATCAGTGAACTGCGCTGGCTGAGTCGTTCACCCAAT
ACGGCTGCGCCAACCCGCACAGTCTTGAAACGGGTGCATGACTTTTTCGACAAGCATCGCCACCATGTACAGTACCGACTGTTTAAGAAACAGGGACTAC
CGATTGGCTCTGGCATGGTCGAGAGTGCGTGTAAGTGGCTCATTCAGCAACGGTTTAAGGGCGTTGGTATGCGCTGGAGTGAACCGGGACTCAATCACTT
GTTACAGTTACGAGTCGCTTGGGGCAATCAACGGTTTGACACGTTGTTTTCTCAACACTCTTTGGTATTGCCTATTATCTCCCCTAACCGCTAGATGCGC
CC
CTTCTATGATAACAACCCCTACGAATAGCTATTCTGAATCAGAAGACAGGTCATCCCGACTTGACCGCCTTCATCAAAGTCAAACCTTAGCGGCTATTGT
GATTGCTGCTTGGCAAATAGGGTTACACGTCGCACGCACGCTCGTCGAACAACAGCTCAATGAGCGTGCTAAACGCCCGACCGATTGGGGGCCTTGTTCA
GTGTGCGGCCAGCGGCTACACAGTAAGGGTTTTGCGACTCGTCAGATGGTGACCTTGGTCGGTCTTGTGCAGTGGCGACGCCGATTAGGTCGCTGCCCTC
AAGGGTGCAAAGGGAGCCACGCGGCTCCCTTTGACCAAACGCTGGGGATTGCCCCGTACCAACAAAGTTCTGTAGAGCTAAAGCGATTAGGTTGCCTGCT
GAGCCTCTTCGTTCCGTTCGAGTTAGCCGCTTGGCTCCTGTCTCAATTGAGTGGCATTCAGGTCAGTGATGACAGCCTGTGGCAGTGGGTCCAACAGTAT
GGTCAACAAGCCGCCTGTCAGTGGGAAACAGATGTTGAGAACTGGGAAAACGGAGAAGACCCGGTGCCCGAAGCACTTTCTGACGACCTCGCGGCAATGC
CGTTGTTGATCGCTGCAGATGGCGTCACGGTCCCACTACGTCCACAGGTCGGCACCCCAGCTGGCAAAACGCTTTGGCGAGAAGTCAAAGTCGGCTTACT
GGCTCGACTCAGTCATCGTATTAATCGCCATGGCAAACTTGTTACTCGCCTACAGCGACGACGTTTGGTCGCCGTGCTAGGCGATATGGCTACGTTTCGG
CCTCGCTTGCAGCTCGAAGCAGCGCGAGCGGGCATCGCAACAGCTCCACACGTTGTCTGGCTCAGCGATGGAGCCAGGGGGTTTTGGCGACTCTTCGAGA
CTTGTTTTTCTCACCTAGCGGTCGGCATCCTCGATTTCTATCATGCAGCGGCCTATCTGTGGCAAGCCGCTGATGCCTACAAAGACGGGAACCCGGCACG
CACACCACAGATGTGGTTTGAGCGATTGCGCCACCAGCTCCGACACGGCTACGTCCACCGGATTATCAGTGAACTGCGCTGGCTGAGTCGTTCACCCAAT
ACGGCTGCGCCAACCCGCACAGTCTTGAAACGGGTGCATGACTTTTTCGACAAGCATCGCCACCATGTACAGTACCGACTGTTTAAGAAACAGGGACTAC
CGATTGGCTCTGGCATGGTCGAGAGTGCGTGTAAGTGGCTCATTCAGCAACGGTTTAAGGGCGTTGGTATGCGCTGGAGTGAACCGGGACTCAATCACTT
GTTACAGTTACGAGTCGCTTGGGGCAATCAACGGTTTGACACGTTGTTTTCTCAACACTCTTTGGTATTGCCTATTATCTCCCCTAACCGCTAGATGCGC
CC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1389 bp | 462 aa | 106 | 1494 | + | No |
Chemistry : DDE
ORF sequence :
MITTPTNSYSESEDRSSRLDRLHQSQTLAAIVIAAWQIGLHVARTLVEQQLNERAKRPTDWGPCSVCGQRLHSKGFATRQMVTLVGLVQWRRRLGRCPQG
CKGSHAAPFDQTLGIAPYQQSSVELKRLGCLLSLFVPFELAAWLLSQLSGIQVSDDSLWQWVQQYGQQAACQWETDVENWENGEDPVPEALSDDLAAMPL
LIAADGVTVPLRPQVGTPAGKTLWREVKVGLLARLSHRINRHGKLVTRLQRRRLVAVLGDMATFRPRLQLEAARAGIATAPHVVWLSDGARGFWRLFETC
FSHLAVGILDFYHAAAYLWQAADAYKDGNPARTPQMWFERLRHQLRHGYVHRIISELRWLSRSPNTAAPTRTVLKRVHDFFDKHRHHVQYRLFKKQGLPI
GSGMVESACKWLIQQRFKGVGMRWSEPGLNHLLQLRVAWGNQRFDTLFSQHSLVLPIISPNR
CKGSHAAPFDQTLGIAPYQQSSVELKRLGCLLSLFVPFELAAWLLSQLSGIQVSDDSLWQWVQQYGQQAACQWETDVENWENGEDPVPEALSDDLAAMPL
LIAADGVTVPLRPQVGTPAGKTLWREVKVGLLARLSHRINRHGKLVTRLQRRRLVAVLGDMATFRPRLQLEAARAGIATAPHVVWLSDGARGFWRLFETC
FSHLAVGILDFYHAAAYLWQAADAYKDGNPARTPQMWFERLRHQLRHGYVHRIISELRWLSRSPNTAAPTRTVLKRVHDFFDKHRHHVQYRLFKKQGLPI
GSGMVESACKWLIQQRFKGVGMRWSEPGLNHLLQLRVAWGNQRFDTLFSQHSLVLPIISPNR
Blast result :
Comments
ISLesp2 is 44% aa similar to ISLfe1.
References
1] ISfinder annotation (2013)
2] Kerfeld,C.A., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J. and Klenk,H.-P. (2012) Direct submission GenBank.
2] Kerfeld,C.A., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J. and Klenk,H.-P. (2012) Direct submission GenBank.