ISfinder Mirror Site

ISMich2

Family ISKra4
Group ISMich2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_DS989857	ND	Microcoleus chthonoplastes	Microcoleus chthonoplastes PCC 7420

DNA section

IS Length : 1805 bp

Ends

IR Length : 23/25

IRL : GGGAGCATCCCATTTTGGCAAATAGCCGGGAAAGGTCGATTTTTAATTCG
IRR : GGGAGCATCCCATTTTGGAAAAGAGAGAAAAGTTATTCGCGACTATAACT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTAGATCCGG	CATTAAGA	GGAGATCCGA	8
AGGTAACACA	AGAATGTG	AGGTTTATAC	8
CTATAACCAA	TGGTCGCA	AGGGAAAGTT	8

DNA sequence

GGGAGCATCCCATTTTGGCAAATAGCCGGGAAAGGTCGATTTTTAATTCGCGGTTATTACTCGCGAATTAAATCTCCGTGTATACTTGAACGACTCAAGG
TGACATAATATAGATGGAGATATTCACAAAGTTTACTGATGAACCAAGCCGAACGCCTGGAACTGGAAGCTTGTTTAAGACGAGCAAGTGAGATTCTGTA
CAACAATAGTGACACAGACAGTTTAGAAACCCTAGCTGACATCGAAATTACAGTCAGAAACCAAATTTTAGAATACGTTAGTCCCCAAATCGCCCTTTTT
TTATCACCCAAAAAACCCAAACCCGACGCGGGAAAACCCGAACCCTAAAAAGCTGCCTGGGAAAACTAAAGATTACCAAAAAACAAGCAGATGCTTTAGG
GATAGAAGCCTATACACGCCATAGCCCTTTGCTAGAAAAATGCTGTCTTCTTTTAAGCGCCAATGAATCTTATCAAAATGCGGAGAGTGATTTGTATTTG
TTAACGGGGCTAAAAGTTGGTCACTCCACGCATCATCGGAAAGTGAATCAAATTGAATTACCGCTTCCGGATCTCAAACAAGGATTATCAGAGATAAGCG
TTGATGGCGGAAAAATTCGCTTGAGGACGGCAGAGAAAGGAGAACCGAGTTTTTGGCAAGAATATAAAACAGCTCGCTTACAAGGAATTTATTACGGAGC
TTTCTTTCAAGATAATCTATCTTTAACGGATTGGATAAATAGTCAAACATTAACCTCACCCTTGTACTGTTTAGGGGATGGACATGATGGGATATGGAAC
ATCTTTGCCGAAATTGGGGAGAATAAGGATAGAATCGAAGTTTTAGACTGGTATCACCTGACCGAAAACCTGCATAAAATAGAAACCAAAAAATCTATCA
AAGACCAGTTAGAAGCTGACCTGTGGACGGGACAGGCATCCGAGGCGATAAAGTTGTTGAAGTCAGCTCAACCCCTAGGAGGAAACAACTTTATTAGCTA
TCTAAACAAGCACCGTCAAAGGTTAATCAATTATCAATCTTATCAACAGGAACAAATTTGTTCAATTGGCAGCGGAGCCGTCGAGTCAGCCGTCAAACAA
ATTAGCCATCGTGTTAAACTAACTGGCGCCCAGTGGCTCAAAGAAAACGTGGTTAACATTTTACAACTTCGTTGTGCTTATTTAAACGGTCAACTGGCTA
TTTAATGCCAACTTCCCTAATTATTAAGCGCATTTTTACAGAGAATTGATATAAGGACTTGAAGTACCGCCCATTTTTGATAAATTCGCGAGTTAGAATG
GCGAATATATCATCATGGAGATAGAAAAATGGGCAAAAGGGTTTCCAGAAAGTCACAACACGATAAGCTGGAAAGAATAACTCGTTTACAGACGGCAATT
GCTAGTCTTGAAACCTACCAAAGTTTCTTTGAGCGTCAAGGAAAACTCGCCCCAGCAGATGCTTGGGTAGCACGTTATCAAGTCCGACAGTCACAAAAAG
TTTACTGGTACTACAAACTACAGGCTAGTTCACCCACTTTCACAACCACAAGTGACACACACAAGTTAAGTAAATATAAGCACTTAGGTAAGGCTGGATG
TGAAGCCCATGTTGCTTCTGTGATGTCTGTCGCCAGAAGAACGATAGTGTCCGAATTACAAAAAACTATTGATTCTCTTAAAAATAGCTTACTAGATATT
TCTTTTGACTCTGAGCAAGAAGATATCTAATATTTAGATTAAGTAATTATTCGCGAGTTATAGTCGCGAATAACTTTTCTCTCTTTTCCAAAATGGGATG
CTCCC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
210 bp	69 aa	139	348	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MNQAERLELEACLRRASEILYNNSDTDSLETLADIEITVRNQILEYVSPQIALFLSPKKPKPDAGKPEP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
924 bp	307 aa	282	1205	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SPNRPFFITQKTQTRRGKTRTLKSCLGKLKITKKQADALGIEAYTRHSPLLEKCCLLLSANESYQNAESDLYLLTGLKVGHSTHHRKVNQIELPLPDLKQ
GLSEISVDGGKIRLRTAEKGEPSFWQEYKTARLQGIYYGAFFQDNLSLTDWINSQTLTSPLYCLGDGHDGIWNIFAEIGENKDRIEVLDWYHLTENLHKI
ETKKSIKDQLEADLWTGQASEAIKLLKSAQPLGGNNFISYLNKHRQRLINYQSYQQEQICSIGSGAVESAVKQISHRVKLTGAQWLKENVVNILQLRCAY
LNGQLAI

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1067 bp	355 aa	139	1205	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNQAERLELEACLRRASEILYNNSDTDSLETLADIEITVRNQILEYVSPQIALFFITQKTQTRRGKTRTLKSCLGKLKITKKQADALGIEAYTRHSPLLE
KCCLLLSANESYQNAESDLYLLTGLKVGHSTHHRKVNQIELPLPDLKQGLSEISVDGGKIRLRTAEKGEPSFWQEYKTARLQGIYYGAFFQDNLSLTDWI
NSQTLTSPLYCLGDGHDGIWNIFAEIGENKDRIEVLDWYHLTENLHKIETKKSIKDQLEADLWTGQASEAIKLLKSAQPLGGNNFISYLNKHRQRLINYQ
SYQQEQICSIGSGAVESAVKQISHRVKLTGAQWLKENVVNILQLRCAYLNGQLAI

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
402 bp	133 aa	1329	1730	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGKRVSRKSQHDKLERITRLQTAIASLETYQSFFERQGKLAPADAWVARYQVRQSQKVYWYYKLQASSPTFTTTSDTHKLSKYKHLGKAGCEAHVASVMS
VARRTIVSELQKTIDSLKNSLLDISFDSEQEDI

Blast result :

Comments

ISMich2 is 83% aa similar to ISCysp26.
The third ORF is the putative ORFAb transposase reconstructed in silico by possible -1 frameshift.
ORF4 is a passenger gene annotated as hypothetical protein.

References

1] ISfinder annotation (2013)
2] Tandeau de Marsac,N., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2008) Direct submission GenBank.