ISOni2

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_019730 ND Oscillatoria nigro-viridis
Oscillatoria nigro-viridis PCC 7112
Oscillatoria nigro-viridis PCC 7112 plasmid pOSC7112.02
DNA section
IS Length : 1179 bp

Ends


IR Length : 23/24

IRL : GGGTGCATCTCACTTTGGCGAATATGTCACCAAAGGGTTAAAATGGAAAA
IRR : GGGTGCATCTCACTTTGGCAAATACATCAAATTGAAAGTTGACCGTTGAG

Insertion site


Left flankDirect repeatRight flankDR Length
AGGGAAGAGAATTGACAGGACTTACGAT8
CTCGTCAGCCCGATCTAAACTATCATCA8
GGATGAGGATGATGACGATGAGGAGGAT8
GTTCTCGAAAAACGTGCAGTTGTCGATC8
CCGAATGTGCGATCGACTTTTGGGAAAA8

DNA sequence

GGGTGCATCTCACTTTGGCGAATATGTCACCAAAGGGTTAAAATGGAAAAGCTAGTAAAATTGCCCAAAAATATGAGGTAAAGAAAATGACACCCTCAGA
CCAAGAACAGCTAAAAGTCTACTTAAAAGCGGCAGCAGAAATTCTTTACAGAAATACAGCTCCAAGCGAGTTAGAAAGCTTTGATAGCATAGAAAAGTCT
CTCCGTCAGAAAATGCTAGAAGAAGTGGGACCAGAACTAGGTAACTTTTTTTTTCAGCAGTATCAGGAATTCAAACAGGAAAACCCAGAAAAATAAAATC
AATAGTCGGTTCGCTCGAAATTACCGACAATCAAGCGAAATATTTTGGATTGAAAGCGTATAGTCAATTCAGTCCAATGATGGAAAAATGCTGTCTTTTA
ATTAGTGCCAACGAATCTTATCAAATGGCAGAAAAAGATTTAGAGATTTTCACCGGAATCAAAGTCTCTCATAGTACATTACAAAGATTAGTCAAACGAC
AAGAATTTGAATTACCTACATCTAAACAAGGAGTTCAAGAAATTACATTAGATGGCGGGAAAGTCAGGCTACGCAACGACACCAAAGGCGAGGGTTGTTA
CTGGAAAGACTATAAAGCCATTTGTTTAGATAATGTTTATTCGGGAGCATCTTTTCAAAATAATCAAAATTTAATTGATTGGACTAATAGCCAAAAATTG
CGACATCCTATGTATTGCCTAGGAGACGGTCATGCGGGAATTTGGAAAATATTTCAAGAAATAGGAGATACTGAACAAAGACAAGAAATCTTAGATTGGT
ATCATCTGAAAGAAAATCTTTACAAGGTAGGAGGTTCGCTAAAACGTTTGAAATTAGCAGAAAATATGTTGTGGCAAGGTAAAATAGATGAAGTTATCAA
TCTATTTAAAGAGATGAAAAAACAAGCATTTAAAACATTTTGTAATTATTTAGAGACTCATCGCTGCCGAATAGTCAACTACCAATACTATAAAGAAGAA
TCCATAAGTTCGATAGGTTCTGGAACAGTTGAATCAATCATTAAACGAATTGGGTTTAGGGTAAAAATATCAGGAGCGCAATGGAAGATTGAGAGCGTTC
CCTCTATCCTTTCTCTTCGCTGTGCTTATCTCAACGGTCAACTTTCAATTTGATGTATTTGCCAAAGTGAGATGCACCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
210 bp69 aa87296+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTPSDQEQLKVYLKAAAEILYRNTAPSELESFDSIEKSLRQKMLEEVGPELGNFFFQQYQEFKQENPEK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
909 bp302 aa2451153+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LFFSAVSGIQTGKPRKIKSIVGSLEITDNQAKYFGLKAYSQFSPMMEKCCLLISANESYQMAEKDLEIFTGIKVSHSTLQRLVKRQEFELPTSKQGVQEI
TLDGGKVRLRNDTKGEGCYWKDYKAICLDNVYSGASFQNNQNLIDWTNSQKLRHPMYCLGDGHAGIWKIFQEIGDTEQRQEILDWYHLKENLYKVGGSLK
RLKLAENMLWQGKIDEVINLFKEMKKQAFKTFCNYLETHRCRIVNYQYYKEESISSIGSGTVESIIKRIGFRVKISGAQWKIESVPSILSLRCAYLNGQL
SI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1067 bp355 aa871153+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTPSDQEQLKVYLKAAAEILYRNTAPSELESFDSIEKSLRQKMLEEVGPELGNFFFSAVSGIQTGKPRKIKSIVGSLEITDNQAKYFGLKAYSQFSPMME
KCCLLISANESYQMAEKDLEIFTGIKVSHSTLQRLVKRQEFELPTSKQGVQEITLDGGKVRLRNDTKGEGCYWKDYKAICLDNVYSGASFQNNQNLIDWT
NSQKLRHPMYCLGDGHAGIWKIFQEIGDTEQRQEILDWYHLKENLYKVGGSLKRLKLAENMLWQGKIDEVINLFKEMKKQAFKTFCNYLETHRCRIVNYQ
YYKEESISSIGSGTVESIIKRIGFRVKISGAQWKIESVPSILSLRCAYLNGQLSI

 

Blast result :
Comments
ISOni2 is 94% aa similar to ISOni1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C., Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and Kerfeld,C. (2012) Direct submission GenBank.