ISLesp1
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_JH993797 | ND | Leptolyngbya sp. | Leptolyngbya sp. PCC 7375 |
DNA section
IS Length : 1165 bp
Ends
IR Length : 15/18
IRL : GGGAACACGTCATCCATTCATCAGAGAAAAAATATCCTAGAGGGTAGTGT
IRR : GGGAGCACGTCATCCGCTGTCGGATGAAAAATAATCGAGTTGATGATTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCTGCTCAA | TGCTCAA | TCCCTTCAAA | 7 |
DNA sequence
GGGAACACGTCATCCATTCATCAGAGAAAAAATATCCTAGAGGGTAGTGTTTCTATCGGTTCACAGTCATGACACCTGAAACCCATCAGCAATTGGATTC
CCATGTGCGAGCAATCGCCCAGATTCTCTATGACGATGCTCAAGCAAACGGGTTACCGATGGATAGCTTGGAGGATATCGAACATACGGTTCGTGCTCAA
CTCCAGAGCCATGTCTCTCCTGGTTTAGGAAATTTTTTATCCAAGCAAATTGTCCAGAAGATGGTGAGCGACCCAGGCGAATAGTGAGCATTCTGGGGGA
ATTGTGGGTCTCTCGTAAACAGGCACAAACACTTTCACTCAAGGCTCGTGTCAAGCACAGTCCGTATCTAGAGAAATGTTGTTTACGGATGTGTGCCAAA
GCGTCCTATGCGCATGCGGAATCGGATGTGGCGATGCTCACGGGAGTTCGTGTGAGTGCCAAGACGCAAGAGCGAATGGTAAAGCGCTCACCGTTACCCG
AGCCAAGGTCTGAAGAGCCCGTCAGCGAACTCACCCTCGATGGCGGGATGGTACGGGTGCGGACCCCCAAGGGTGAAGCCAGCGTCTGGCGTCAGTATCA
CGGCATCAGAGTCAATGGCGATGGCCTCGGGATGGCTTGGTACAAAGACCCAGCGACCTTGTTGGCATGGGTGACGACATTGCCTCTCGTCCCTGTGATT
TATCTGCTGGGTGATGGGCACTGTGGCATTTGGTCCCTATTTGACCAGATGGCAATAGAGGTCCCAACCGACGAAGTGCTTGATTGGTATCACCTGAAAG
AGAATCTCTACAAAGTCGAGGCCCCAGCACCGGTGCTGGAATCGCTATCGAGTCAACTATGGGAAGGGCGACTCCAACAGGTTTTGGTTTGCCTATCAGA
GTTGGACAATGACTCGGCCCGACGCTTTAGTGCCTATCTGAACAACCATGCTGGACGCATTCCCAACTATCGCTATTATCAGATGGAAGGCTTGCCCATT
GGCTCAGGTCCTGTTGAGTCTTGGGTCAAACAGATTGATGCCAGATTGCAGGTGACGGGAGCTCAATGGAATGAAGAGACGTTGCCACAAATGCTCAAAT
TGAGATCCGTTTATCTAAATCATCAACTCGATTATTTTTCATCCGACAGCGGATGACGTGCTCCC
CCATGTGCGAGCAATCGCCCAGATTCTCTATGACGATGCTCAAGCAAACGGGTTACCGATGGATAGCTTGGAGGATATCGAACATACGGTTCGTGCTCAA
CTCCAGAGCCATGTCTCTCCTGGTTTAGGAAATTTTTTATCCAAGCAAATTGTCCAGAAGATGGTGAGCGACCCAGGCGAATAGTGAGCATTCTGGGGGA
ATTGTGGGTCTCTCGTAAACAGGCACAAACACTTTCACTCAAGGCTCGTGTCAAGCACAGTCCGTATCTAGAGAAATGTTGTTTACGGATGTGTGCCAAA
GCGTCCTATGCGCATGCGGAATCGGATGTGGCGATGCTCACGGGAGTTCGTGTGAGTGCCAAGACGCAAGAGCGAATGGTAAAGCGCTCACCGTTACCCG
AGCCAAGGTCTGAAGAGCCCGTCAGCGAACTCACCCTCGATGGCGGGATGGTACGGGTGCGGACCCCCAAGGGTGAAGCCAGCGTCTGGCGTCAGTATCA
CGGCATCAGAGTCAATGGCGATGGCCTCGGGATGGCTTGGTACAAAGACCCAGCGACCTTGTTGGCATGGGTGACGACATTGCCTCTCGTCCCTGTGATT
TATCTGCTGGGTGATGGGCACTGTGGCATTTGGTCCCTATTTGACCAGATGGCAATAGAGGTCCCAACCGACGAAGTGCTTGATTGGTATCACCTGAAAG
AGAATCTCTACAAAGTCGAGGCCCCAGCACCGGTGCTGGAATCGCTATCGAGTCAACTATGGGAAGGGCGACTCCAACAGGTTTTGGTTTGCCTATCAGA
GTTGGACAATGACTCGGCCCGACGCTTTAGTGCCTATCTGAACAACCATGCTGGACGCATTCCCAACTATCGCTATTATCAGATGGAAGGCTTGCCCATT
GGCTCAGGTCCTGTTGAGTCTTGGGTCAAACAGATTGATGCCAGATTGCAGGTGACGGGAGCTCAATGGAATGAAGAGACGTTGCCACAAATGCTCAAAT
TGAGATCCGTTTATCTAAATCATCAACTCGATTATTTTTCATCCGACAGCGGATGACGTGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 216 bp | 71 aa | 69 | 284 | + | No |
Description : First part of the transposase
ORF sequence :
MTPETHQQLDSHVRAIAQILYDDAQANGLPMDSLEDIEHTVRAQLQSHVSPGLGNFLSKQIVQKMVSDPGE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 990 bp | 329 aa | 167 | 1156 | + | No |
Description : Second part of the transposase
ORF sequence :
LGGYRTYGSCSTPEPCLSWFRKFFIQANCPEDGERPRRIVSILGELWVSRKQAQTLSLKARVKHSPYLEKCCLRMCAKASYAHAESDVAMLTGVRVSAKT
QERMVKRSPLPEPRSEEPVSELTLDGGMVRVRTPKGEASVWRQYHGIRVNGDGLGMAWYKDPATLLAWVTTLPLVPVIYLLGDGHCGIWSLFDQMAIEVP
TDEVLDWYHLKENLYKVEAPAPVLESLSSQLWEGRLQQVLVCLSELDNDSARRFSAYLNNHAGRIPNYRYYQMEGLPIGSGPVESWVKQIDARLQVTGAQ
WNEETLPQMLKLRSVYLNHQLDYFSSDSG
QERMVKRSPLPEPRSEEPVSELTLDGGMVRVRTPKGEASVWRQYHGIRVNGDGLGMAWYKDPATLLAWVTTLPLVPVIYLLGDGHCGIWSLFDQMAIEVP
TDEVLDWYHLKENLYKVEAPAPVLESLSSQLWEGRLQQVLVCLSELDNDSARRFSAYLNNHAGRIPNYRYYQMEGLPIGSGPVESWVKQIDARLQVTGAQ
WNEETLPQMLKLRSVYLNHQLDYFSSDSG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1088 bp | 362 aa | 69 | 1156 | + | Yes |
Chemistry : DDE
ORF sequence :
MTPETHQQLDSHVRAIAQILYDDAQANGLPMDSLEDIEHTVRAQLQSHVSPGLGNFFIQANCPEDGERPRRIVSILGELWVSRKQAQTLSLKARVKHSPY
LEKCCLRMCAKASYAHAESDVAMLTGVRVSAKTQERMVKRSPLPEPRSEEPVSELTLDGGMVRVRTPKGEASVWRQYHGIRVNGDGLGMAWYKDPATLLA
WVTTLPLVPVIYLLGDGHCGIWSLFDQMAIEVPTDEVLDWYHLKENLYKVEAPAPVLESLSSQLWEGRLQQVLVCLSELDNDSARRFSAYLNNHAGRIPN
YRYYQMEGLPIGSGPVESWVKQIDARLQVTGAQWNEETLPQMLKLRSVYLNHQLDYFSSDSG
LEKCCLRMCAKASYAHAESDVAMLTGVRVSAKTQERMVKRSPLPEPRSEEPVSELTLDGGMVRVRTPKGEASVWRQYHGIRVNGDGLGMAWYKDPATLLA
WVTTLPLVPVIYLLGDGHCGIWSLFDQMAIEVPTDEVLDWYHLKENLYKVEAPAPVLESLSSQLWEGRLQQVLVCLSELDNDSARRFSAYLNNHAGRIPN
YRYYQMEGLPIGSGPVESWVKQIDARLQVTGAQWNEETLPQMLKLRSVYLNHQLDYFSSDSG
Blast result :
Comments
ISLesp1 is 68% aa similar to ISCysp24.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Kerfeld,C.A., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J. and Klenk,H.-P. (2012) Direct submission GenBank.
2] Kerfeld,C.A., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T., Eisen,J. and Klenk,H.-P. (2012) Direct submission GenBank.