ISCysp26

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAXW01000006 ND Cyanothece sp.
Cyanothece sp. CCY0110
DNA section
IS Length : 1758 bp

Ends


IR Length : 20

IRL : GGGAGCATCCCATTTTTGTATTTAGACTGTTTAATACGATGCAAATACTT
IRR : GGGAGCATCCCATTTTTGTAGAAACAAAGCATTATTCGCGGAAACTATTC

Insertion site


Left flankDirect repeatRight flankDR Length
CTTTTTTAATTCTTTTTGTGCTGATTTT8

DNA sequence

GGGAGCATCCCATTTTTGTATTTAGACTGTTTAATACGATGCAAATACTTCGCGAAGAACCATCGCGAATAAGTTTAAATGTTTATGCAATCGAATTAAT
TCTGACATAATGGGGGTGTAAACATTGAAGATTATCCCGTTATGACTCCATCAGAAAGACTTGAGTTAGAAGCCTGTATTAACAGAGCCAGTGAAATACT
TTATAATAATGTTGAAGAAGAATCTTTAAAGACTCTTGAAGATATTGAAATTACTGTAAGGGAGCAGGTTTTAGAGAATGTTAGTCCTCAAATCACCCTT
TTTTTATTGAAAGAAAAACAAAAACGAGAAAAGGAAGAAAGCGAAAAATAAAAAGTTGTGTGGGACAGTTAAAAATTACTGCATCTCAATGTAATCGGTT
GGGTGTTAGTTCCTATACTCGTCATAGTCCCCTCTTAGAAAAGTGTTGTTTACTGTTAAGTGCTAACGAATCTTATCAAAATGCTGAAGAGGATATGTTA
TTATTAATGGGCTTTAAAGTGGGGCATTCAACTCATCACCGAAAAGTCCAAAAACTAGATAATTTATTACCAGATGTTAAACAAGGTTGTAGTGAGATAT
CTGTTGATGGAGGAAAAATTAGGTTAAGAGGAAAAAAGGGACAAAAAAGTTATTGGAAAGATTATAAAACGGCGAGATTACAAGGAATCTATTACGGTGC
TTTTTTTCAGGATAATCAATCTTTAGTCGATTGGATTAATAGCCAAGAATTAACCAATCCACTTTATTGTTTAGGAGATGGACATGATGGAATATGGAAT
ATAATGGCTGAAATTGGAGATAAAGAAAATAGGATTGAGATTTTGGATTGGTATCATTTAATAGAAAATCTCTACAAAGTTGAAGGGAAAAGATACCAGT
TTGAACAAATTAAGGCTTATTTATGGATGGGGCAGACCTCAGAGGCGATTAGCTATCTACGTGACCAGAACCCAGTGGGGAGTAATCAGTTTTGCAACTA
TCTAATTAAACGGAAATCTCGCATCATTAATTACCATTATTATAGCTGGCAAAAAATTTGTTCAATCGGTAGCGGGGCTGTAGAATCGGCGGTTAAACAA
ATAGCTCACCGCGTCAAAATAACCGGCGCACAGTGGAAATATGAAACCGTTAATAATATTCTACAACTGCGTTGTGCTTATCTCAATGGTCAACTAGCTA
TTTAAAACGGATAAAATTGTAAAAGTTAAATAAGTTGCTTATTAATTCGCGTATTATAATGGCGAATTACTTCAACATGGGGTTTAATATTGATGGGACG
CAAAAAATATATCGAGAGTGAGCAAGATAAATTAGATAGAATGCAGAGATTAACCGAAGCTATTGCTGAAATAGAGTCATATTTGCAGTTTTTTGAGGAT
AAGGGAGAACAAGCTGATAGTGGTTGTTGGGTCGTTCGTTATCAAGTGAGGCAAAAAGACAAAATTTATTGGTATTACAAGTTACAAGCAGATAGACGTA
TATTTGAGCAGAAAAACAGTCAATCTCTTAGCAAATACCAACATTTAGGCAAAGCTGGTACAATTGCCCATGTCGATGCTGTCATGGGAGTCCTCAGACG
GACTATCATCACCGAATTAGAGTCTACCTTGATTTCTCTGAAAAGCTGTGTAGTTGATATCGCTTATGATGAAGAACATAAAAGTAGATAACTAGAAATT
TATTTCGCGAATAGTTTCCGCGAATAATGCTTTGTTTCTACAAAAATGGGATGCTCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 4

 

ORF 1
LengthBeginEndStrandFusion ORF
210 bp69 aa142351+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTPSERLELEACINRASEILYNNVEEESLKTLEDIEITVREQVLENVSPQITLFLLKEKQKREKEESEK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
921 bp306 aa2851205+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SSNHPFFIERKTKTRKGRKRKIKSCVGQLKITASQCNRLGVSSYTRHSPLLEKCCLLLSANESYQNAEEDMLLLMGFKVGHSTHHRKVQKLDNLLPDVKQ
GCSEISVDGGKIRLRGKKGQKSYWKDYKTARLQGIYYGAFFQDNQSLVDWINSQELTNPLYCLGDGHDGIWNIMAEIGDKENRIEILDWYHLIENLYKVE
GKRYQFEQIKAYLWMGQTSEAISYLRDQNPVGSNQFCNYLIKRKSRIINYHYYSWQKICSIGSGAVESAVKQIAHRVKITGAQWKYETVNNILQLRCAYL
NGQLAI

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1064 bp354 aa1421205+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTPSERLELEACINRASEILYNNVEEESLKTLEDIEITVREQVLENVSPQITLFFIERKTKTRKGRKRKIKSCVGQLKITASQCNRLGVSSYTRHSPLLE
KCCLLLSANESYQNAEEDMLLLMGFKVGHSTHHRKVQKLDNLLPDVKQGCSEISVDGGKIRLRGKKGQKSYWKDYKTARLQGIYYGAFFQDNQSLVDWIN
SQELTNPLYCLGDGHDGIWNIMAEIGDKENRIEILDWYHLIENLYKVEGKRYQFEQIKAYLWMGQTSEAISYLRDQNPVGSNQFCNYLIKRKSRIINYHY
YSWQKICSIGSGAVESAVKQIAHRVKITGAQWKYETVNNILQLRCAYLNGQLAI

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
399 bp132 aa12931691+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGRKKYIESEQDKLDRMQRLTEAIAEIESYLQFFEDKGEQADSGCWVVRYQVRQKDKIYWYYKLQADRRIFEQKNSQSLSKYQHLGKAGTIAHVDAVMGV
LRRTIITELESTLISLKSCVVDIAYDEEHKSR

 

Blast result :
Comments
ISCysp26 is 74% aa similar to ISMic1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ORF4 is a passenger gene annotated as hypothetical protein.
References
1] ISfinder annotation (2013)
2] Stal,L., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2007) Direct submission GenBank.