ISPle1
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_019689 | ND | Pleurocapsa sp. | Pleurocapsa sp. PCC 7327 |
DNA section
IS Length : 1172 bp
Ends
IR Length : 21/22
IRL : GGGAGCATCCAGTTTTGGAAGATTTACCATTTATGGGAAAATGTAAAGGG
IRR : GGGAGCATCCACTTTTGGAAGAAACACCCAATCAGACAGCAAGTAAACCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGTCGATTTT | AATCGAGT | TGAGCTTTGA | 8 |
DNA sequence
GGGAGCATCCAGTTTTGGAAGATTTACCATTTATGGGAAAATGTAAAGGGAAATAATCCTGTGCGATCGCTGACAATGAACCAAGAGAAACAAGAACGGA
TCAAAGCCTGCTTACAAGAATTGTCAACACTGCTGTATGAAGAAGCAGACAAAAGTAAGCTGACAGACCTTGAAGGCATAGAAAAAACAGTTCGCAGTCA
AGTATTAGAACTAGTCAGCCCAGAAATAGCCCTTTTTTATCGAACAAAAAACTGGAACAAAAGTAGGTAAAACTAGGAAAATAAAAAGCCTAGTAGGGGA
ACTGAAATTAAAAGCCAAACAGCTTATTAGACTAGGTTTAAAGCCAAGAACTCGCCTAAGTCCATTACTTCAAAAGTGCTGTTTGAGGTTATCAGCTAAC
GAATCATACCAAAAAGCAGAAATCGAGATTGAGGCATTGACAGGAGTAAAAGTTGGTCATTCAACACAACAACAACTAGTGCTGTCACAAGATTTTCAAC
TACCACTTGCTAAACAATCAGTTTCAGAAGTCAGCGTAGATGGAGGAAAAGTCCGACTCAGGGGTAAACCGAAAGCAGGCTGCCACTGGCGAGACTATAA
AACCGTTCGTCTGCAAGGGATTTACTATGGTGCATTTTTTGATGACAACCAATCATTAATTGATTATGTCAATAGCCAACAGTTGTTAGACCCTCTTGTT
TGCTTGGGAGATGGACATGATGGTGTCTGGAATCTGGTGAGAGAATTTGGACAAGACCAGTTTTCTCGCTGTGAAATTTTGGATTGGTATCACCTGAAGG
AAAATCTTTATAAAGTTGGTGGTTCTTTAAAGCGACTCAAAGCTGCGGAAACTCTGTTGTGGCAAGGTCAGGTAGAAGCGGCCAAGACCCTATTTAATCA
TTGCCGAGGTAAACAAGCTAAAAATTTCATCGCTTATCTGGAAAAACATCTCCCTCGCATTGTCAATTACAGCTATTATCAGGCTGAACAATTATGTTCT
ATCGGTTCAGGGGCAGTTGAATCTGCAATTAAACAAATTGGTTTAAGGATCAAAATTTCTGGCGCACAGTGGAATGTTGAAAGTGTCAATCACATCCTCT
CTGTTCGTTGTGCTTATCTTAATGGTTTACTTGCTGTCTGATTGGGTGTTTCTTCCAAAAGTGGATGCTCCC
TCAAAGCCTGCTTACAAGAATTGTCAACACTGCTGTATGAAGAAGCAGACAAAAGTAAGCTGACAGACCTTGAAGGCATAGAAAAAACAGTTCGCAGTCA
AGTATTAGAACTAGTCAGCCCAGAAATAGCCCTTTTTTATCGAACAAAAAACTGGAACAAAAGTAGGTAAAACTAGGAAAATAAAAAGCCTAGTAGGGGA
ACTGAAATTAAAAGCCAAACAGCTTATTAGACTAGGTTTAAAGCCAAGAACTCGCCTAAGTCCATTACTTCAAAAGTGCTGTTTGAGGTTATCAGCTAAC
GAATCATACCAAAAAGCAGAAATCGAGATTGAGGCATTGACAGGAGTAAAAGTTGGTCATTCAACACAACAACAACTAGTGCTGTCACAAGATTTTCAAC
TACCACTTGCTAAACAATCAGTTTCAGAAGTCAGCGTAGATGGAGGAAAAGTCCGACTCAGGGGTAAACCGAAAGCAGGCTGCCACTGGCGAGACTATAA
AACCGTTCGTCTGCAAGGGATTTACTATGGTGCATTTTTTGATGACAACCAATCATTAATTGATTATGTCAATAGCCAACAGTTGTTAGACCCTCTTGTT
TGCTTGGGAGATGGACATGATGGTGTCTGGAATCTGGTGAGAGAATTTGGACAAGACCAGTTTTCTCGCTGTGAAATTTTGGATTGGTATCACCTGAAGG
AAAATCTTTATAAAGTTGGTGGTTCTTTAAAGCGACTCAAAGCTGCGGAAACTCTGTTGTGGCAAGGTCAGGTAGAAGCGGCCAAGACCCTATTTAATCA
TTGCCGAGGTAAACAAGCTAAAAATTTCATCGCTTATCTGGAAAAACATCTCCCTCGCATTGTCAATTACAGCTATTATCAGGCTGAACAATTATGTTCT
ATCGGTTCAGGGGCAGTTGAATCTGCAATTAAACAAATTGGTTTAAGGATCAAAATTTCTGGCGCACAGTGGAATGTTGAAAGTGTCAATCACATCCTCT
CTGTTCGTTGTGCTTATCTTAATGGTTTACTTGCTGTCTGATTGGGTGTTTCTTCCAAAAGTGGATGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 195 bp | 64 aa | 76 | 270 | + | No |
Description : First part of the transposase
ORF sequence :
MNQEKQERIKACLQELSTLLYEEADKSKLTDLEGIEKTVRSQVLELVSPEIALFYRTKNWNKSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 303 aa | 230 | 1141 | + | No |
Description : Second part of the transposase
ORF sequence :
PFFIEQKTGTKVGKTRKIKSLVGELKLKAKQLIRLGLKPRTRLSPLLQKCCLRLSANESYQKAEIEIEALTGVKVGHSTQQQLVLSQDFQLPLAKQSVSE
VSVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVNSQQLLDPLVCLGDGHDGVWNLVREFGQDQFSRCEILDWYHLKENLYKVGGSL
KRLKAAETLLWQGQVEAAKTLFNHCRGKQAKNFIAYLEKHLPRIVNYSYYQAEQLCSIGSGAVESAIKQIGLRIKISGAQWNVESVNHILSVRCAYLNGL
LAV
VSVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVNSQQLLDPLVCLGDGHDGVWNLVREFGQDQFSRCEILDWYHLKENLYKVGGSL
KRLKAAETLLWQGQVEAAKTLFNHCRGKQAKNFIAYLEKHLPRIVNYSYYQAEQLCSIGSGAVESAIKQIGLRIKISGAQWNVESVNHILSVRCAYLNGL
LAV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1066 bp | 355 aa | 76 | 1141 | + | Yes |
Chemistry : DDE
ORF sequence :
MNQEKQERIKACLQELSTLLYEEADKSKLTDLEGIEKTVRSQVLELVSPEIALFFIEQKTGTKVGKTRKIKSLVGELKLKAKQLIRLGLKPRTRLSPLLQ
KCCLRLSANESYQKAEIEIEALTGVKVGHSTQQQLVLSQDFQLPLAKQSVSEVSVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVN
SQQLLDPLVCLGDGHDGVWNLVREFGQDQFSRCEILDWYHLKENLYKVGGSLKRLKAAETLLWQGQVEAAKTLFNHCRGKQAKNFIAYLEKHLPRIVNYS
YYQAEQLCSIGSGAVESAIKQIGLRIKISGAQWNVESVNHILSVRCAYLNGLLAV
KCCLRLSANESYQKAEIEIEALTGVKVGHSTQQQLVLSQDFQLPLAKQSVSEVSVDGGKVRLRGKPKAGCHWRDYKTVRLQGIYYGAFFDDNQSLIDYVN
SQQLLDPLVCLGDGHDGVWNLVREFGQDQFSRCEILDWYHLKENLYKVGGSLKRLKAAETLLWQGQVEAAKTLFNHCRGKQAKNFIAYLEKHLPRIVNYS
YYQAEQLCSIGSGAVESAIKQIGLRIKISGAQWNVESVNHILSVRCAYLNGLLAV
Blast result :
Comments
ISPle1 is 94% aa similar to ISNpu14.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C.,
Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and Kerfeld,C. (2012) Direct submission GenBank.
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Davenport,K., Daligault,H., Erkkila,T., Gu,W., Munk,A.C.C.,
Teshima,H., Xu,Y., Chain,P., Chen,A., Krypides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Mikhailova,N., Ovchinnikova,G., Pagani,I., Pati,A., Goodwin,L., Peters,L., Pitluck,S., Woyke,T. and Kerfeld,C. (2012) Direct submission GenBank.