ISNsp5

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003240 ND Nostoc sp.
Nostoc sp. PCC 7120 plasmid pCC7120beta
DNA section
IS Length : 1166 bp

Ends


IR Length : 18

IRL : GGGAGCATCTCACTTTTTTGCAAAGAGGTTAAAAATCAGTCAGGATAAGT
IRR : GGGAGCATCTCACTTTTTAAAGTGGCTCAAACAAACAATAATCCATTGAG

Insertion site


Left flankDirect repeatRight flankDR Length
ATTTCGGCTATTTGATGATCGCATTCAAG9

DNA sequence

GGGAGCATCTCACTTTTTTGCAAAGAGGTTAAAAATCAGTCAGGATAAGTAAGATGAGTGTATTTACTTAACGATGACTCCAGAACAAAAGCAAGCTCTT
CAAGAACATGTCAAAGCGATAGCTAAAATATTGTACGAAGACACGTCACCAGAAAGGCTAACGAGCCTTGCAGGAATTGAACAAGCAGTGCGGAATCAAA
TGCAGAAGCATGTAATGCCAGAAGTAGGGGTTTTTTTGTCGAAGCAGTTACAGGAACCACCGCAGGATACCAACGACGACTCAAAAGTATCCTGGGAGAG
TTACCAATAACGAGCAAGCAAGCTATTGAGCTAGAAGTCGAGCCAAGTAGCCAACTGAGTCCATATCTAGAAACTTGTTGTTTACGGGTGAGCGCGAATG
TCAGCTATGAAGATGCGGCATCAGATATCGAGTATTTTACGGGTGTATATATCTCCCGCAGCAGTCAACAGAGATTGGTACATCGTCAGAAATTTGAATT
ACCAACACAAGAACATACAGTTGAAGAATTAAGTGTTGATGGTGGAAATATCCGCATTCGGACTCAATCAGGAGAAGTATGTGCGTGGCTTGGCTATAAA
GCGATTAGCTTACATCATCTCAAAACTGTTGGAACGTCCTTTCAAGATAATCAGGTTGTGATTGATTGGGTGAATGACCAACTACTGGCTAGTACAGTCA
CTTGTATTGGTGATGGGCATGACGGCATTTGGAATATTATTGACCAATTAGCACCTGATGTACAACGTCGAGAAGTACTTGATTGGTTTCATTTGATAGA
AAACCTTCACAAAATTGGGGGTTCACAAAAACGCTTGAAACAGGCACAAGCCTTACTATGGAAAGGTCAAGTCGAAGAAGCTAAAGCCTTATTTACTGGT
TGTCAGGGTAAACAAGTACAAAACTTTTGTCGTTATCTCGATAAGCATCGTCATCGCATCATCAACTACGAATATCATCAAGCTGAAGAAATTTGTTCCA
TTGGTTCTGGGTCTGTTGAATCTGCCGTTAAACAGATTGACCGTCGAACAAAGATTTCTGGGGCGCAATGGAAACGAGAAAATGTGCCTCAAGTTCTGGC
TCATCGCTGTGCTTACCTCAATGGATTATTGTTTGTTTGAGCCACTTTAAAAAGTGAGATGCTCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
237 bp78 aa74310+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTPEQKQALQEHVKAIAKILYEDTSPERLTSLAGIEQAVRNQMQKHVMPEVGVFLSKQLQEPPQDTNDDSKVSWESYQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
960 bp319 aa1811140+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TSSAESNAEACNARSRGFFVEAVTGTTAGYQRRLKSILGELPITSKQAIELEVEPSSQLSPYLETCCLRVSANVSYEDAASDIEYFTGVYISRSSQQRLV
HRQKFELPTQEHTVEELSVDGGNIRIRTQSGEVCAWLGYKAISLHHLKTVGTSFQDNQVVIDWVNDQLLASTVTCIGDGHDGIWNIIDQLAPDVQRREVL
DWFHLIENLHKIGGSQKRLKQAQALLWKGQVEEAKALFTGCQGKQVQNFCRYLDKHRHRIINYEYHQAEEICSIGSGSVESAVKQIDRRTKISGAQWKRE
NVPQVLAHRCAYLNGLLFV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1067 bp355 aa741140+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTPEQKQALQEHVKAIAKILYEDTSPERLTSLAGIEQAVRNQMQKHVMPEVGVFFVEAVTGTTAGYQRRLKSILGELPITSKQAIELEVEPSSQLSPYLE
TCCLRVSANVSYEDAASDIEYFTGVYISRSSQQRLVHRQKFELPTQEHTVEELSVDGGNIRIRTQSGEVCAWLGYKAISLHHLKTVGTSFQDNQVVIDWV
NDQLLASTVTCIGDGHDGIWNIIDQLAPDVQRREVLDWFHLIENLHKIGGSQKRLKQAQALLWKGQVEEAKALFTGCQGKQVQNFCRYLDKHRHRIINYE
YHQAEEICSIGSGSVESAVKQIDRRTKISGAQWKRENVPQVLAHRCAYLNGLLFV

 

Blast result :
Comments
ISNsp5 is 82% aa similar to ISNpu15.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Kaneko,T., Nakamura,Y., Wolk,C.P., Kuritz,T., Sasamoto,S.,
Watanabe,A., Iriguchi,M., Ishikawa,A., Kawashima,K., Kimura,T.,
Kishida,Y., Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A.,
Nakazaki,N., Shimpo,S., Sugimoto,M., Takazawa,M., Yamada,M., Yasuda,M. and Tabata,S. (2001) DNA Res. 8 (5), 205-213.