ISNsp5
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_003240 | ND | Nostoc sp. | Nostoc sp. PCC 7120 plasmid pCC7120beta |
DNA section
IS Length : 1166 bp
Ends
IR Length : 18
IRL : GGGAGCATCTCACTTTTTTGCAAAGAGGTTAAAAATCAGTCAGGATAAGT
IRR : GGGAGCATCTCACTTTTTAAAGTGGCTCAAACAAACAATAATCCATTGAG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
ATTTCGGCTA | TTTGATGAT | CGCATTCAAG | 9 |
DNA sequence
GGGAGCATCTCACTTTTTTGCAAAGAGGTTAAAAATCAGTCAGGATAAGTAAGATGAGTGTATTTACTTAACGATGACTCCAGAACAAAAGCAAGCTCTT
CAAGAACATGTCAAAGCGATAGCTAAAATATTGTACGAAGACACGTCACCAGAAAGGCTAACGAGCCTTGCAGGAATTGAACAAGCAGTGCGGAATCAAA
TGCAGAAGCATGTAATGCCAGAAGTAGGGGTTTTTTTGTCGAAGCAGTTACAGGAACCACCGCAGGATACCAACGACGACTCAAAAGTATCCTGGGAGAG
TTACCAATAACGAGCAAGCAAGCTATTGAGCTAGAAGTCGAGCCAAGTAGCCAACTGAGTCCATATCTAGAAACTTGTTGTTTACGGGTGAGCGCGAATG
TCAGCTATGAAGATGCGGCATCAGATATCGAGTATTTTACGGGTGTATATATCTCCCGCAGCAGTCAACAGAGATTGGTACATCGTCAGAAATTTGAATT
ACCAACACAAGAACATACAGTTGAAGAATTAAGTGTTGATGGTGGAAATATCCGCATTCGGACTCAATCAGGAGAAGTATGTGCGTGGCTTGGCTATAAA
GCGATTAGCTTACATCATCTCAAAACTGTTGGAACGTCCTTTCAAGATAATCAGGTTGTGATTGATTGGGTGAATGACCAACTACTGGCTAGTACAGTCA
CTTGTATTGGTGATGGGCATGACGGCATTTGGAATATTATTGACCAATTAGCACCTGATGTACAACGTCGAGAAGTACTTGATTGGTTTCATTTGATAGA
AAACCTTCACAAAATTGGGGGTTCACAAAAACGCTTGAAACAGGCACAAGCCTTACTATGGAAAGGTCAAGTCGAAGAAGCTAAAGCCTTATTTACTGGT
TGTCAGGGTAAACAAGTACAAAACTTTTGTCGTTATCTCGATAAGCATCGTCATCGCATCATCAACTACGAATATCATCAAGCTGAAGAAATTTGTTCCA
TTGGTTCTGGGTCTGTTGAATCTGCCGTTAAACAGATTGACCGTCGAACAAAGATTTCTGGGGCGCAATGGAAACGAGAAAATGTGCCTCAAGTTCTGGC
TCATCGCTGTGCTTACCTCAATGGATTATTGTTTGTTTGAGCCACTTTAAAAAGTGAGATGCTCCC
CAAGAACATGTCAAAGCGATAGCTAAAATATTGTACGAAGACACGTCACCAGAAAGGCTAACGAGCCTTGCAGGAATTGAACAAGCAGTGCGGAATCAAA
TGCAGAAGCATGTAATGCCAGAAGTAGGGGTTTTTTTGTCGAAGCAGTTACAGGAACCACCGCAGGATACCAACGACGACTCAAAAGTATCCTGGGAGAG
TTACCAATAACGAGCAAGCAAGCTATTGAGCTAGAAGTCGAGCCAAGTAGCCAACTGAGTCCATATCTAGAAACTTGTTGTTTACGGGTGAGCGCGAATG
TCAGCTATGAAGATGCGGCATCAGATATCGAGTATTTTACGGGTGTATATATCTCCCGCAGCAGTCAACAGAGATTGGTACATCGTCAGAAATTTGAATT
ACCAACACAAGAACATACAGTTGAAGAATTAAGTGTTGATGGTGGAAATATCCGCATTCGGACTCAATCAGGAGAAGTATGTGCGTGGCTTGGCTATAAA
GCGATTAGCTTACATCATCTCAAAACTGTTGGAACGTCCTTTCAAGATAATCAGGTTGTGATTGATTGGGTGAATGACCAACTACTGGCTAGTACAGTCA
CTTGTATTGGTGATGGGCATGACGGCATTTGGAATATTATTGACCAATTAGCACCTGATGTACAACGTCGAGAAGTACTTGATTGGTTTCATTTGATAGA
AAACCTTCACAAAATTGGGGGTTCACAAAAACGCTTGAAACAGGCACAAGCCTTACTATGGAAAGGTCAAGTCGAAGAAGCTAAAGCCTTATTTACTGGT
TGTCAGGGTAAACAAGTACAAAACTTTTGTCGTTATCTCGATAAGCATCGTCATCGCATCATCAACTACGAATATCATCAAGCTGAAGAAATTTGTTCCA
TTGGTTCTGGGTCTGTTGAATCTGCCGTTAAACAGATTGACCGTCGAACAAAGATTTCTGGGGCGCAATGGAAACGAGAAAATGTGCCTCAAGTTCTGGC
TCATCGCTGTGCTTACCTCAATGGATTATTGTTTGTTTGAGCCACTTTAAAAAGTGAGATGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
237 bp | 78 aa | 74 | 310 | + | No |
Description : First part of the transposase
ORF sequence :
MTPEQKQALQEHVKAIAKILYEDTSPERLTSLAGIEQAVRNQMQKHVMPEVGVFLSKQLQEPPQDTNDDSKVSWESYQ
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
960 bp | 319 aa | 181 | 1140 | + | No |
Description : Second part of the transposase
ORF sequence :
TSSAESNAEACNARSRGFFVEAVTGTTAGYQRRLKSILGELPITSKQAIELEVEPSSQLSPYLETCCLRVSANVSYEDAASDIEYFTGVYISRSSQQRLV
HRQKFELPTQEHTVEELSVDGGNIRIRTQSGEVCAWLGYKAISLHHLKTVGTSFQDNQVVIDWVNDQLLASTVTCIGDGHDGIWNIIDQLAPDVQRREVL
DWFHLIENLHKIGGSQKRLKQAQALLWKGQVEEAKALFTGCQGKQVQNFCRYLDKHRHRIINYEYHQAEEICSIGSGSVESAVKQIDRRTKISGAQWKRE
NVPQVLAHRCAYLNGLLFV
HRQKFELPTQEHTVEELSVDGGNIRIRTQSGEVCAWLGYKAISLHHLKTVGTSFQDNQVVIDWVNDQLLASTVTCIGDGHDGIWNIIDQLAPDVQRREVL
DWFHLIENLHKIGGSQKRLKQAQALLWKGQVEEAKALFTGCQGKQVQNFCRYLDKHRHRIINYEYHQAEEICSIGSGSVESAVKQIDRRTKISGAQWKRE
NVPQVLAHRCAYLNGLLFV
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1067 bp | 355 aa | 74 | 1140 | + | Yes |
Chemistry : DDE
ORF sequence :
MTPEQKQALQEHVKAIAKILYEDTSPERLTSLAGIEQAVRNQMQKHVMPEVGVFFVEAVTGTTAGYQRRLKSILGELPITSKQAIELEVEPSSQLSPYLE
TCCLRVSANVSYEDAASDIEYFTGVYISRSSQQRLVHRQKFELPTQEHTVEELSVDGGNIRIRTQSGEVCAWLGYKAISLHHLKTVGTSFQDNQVVIDWV
NDQLLASTVTCIGDGHDGIWNIIDQLAPDVQRREVLDWFHLIENLHKIGGSQKRLKQAQALLWKGQVEEAKALFTGCQGKQVQNFCRYLDKHRHRIINYE
YHQAEEICSIGSGSVESAVKQIDRRTKISGAQWKRENVPQVLAHRCAYLNGLLFV
TCCLRVSANVSYEDAASDIEYFTGVYISRSSQQRLVHRQKFELPTQEHTVEELSVDGGNIRIRTQSGEVCAWLGYKAISLHHLKTVGTSFQDNQVVIDWV
NDQLLASTVTCIGDGHDGIWNIIDQLAPDVQRREVLDWFHLIENLHKIGGSQKRLKQAQALLWKGQVEEAKALFTGCQGKQVQNFCRYLDKHRHRIINYE
YHQAEEICSIGSGSVESAVKQIDRRTKISGAQWKRENVPQVLAHRCAYLNGLLFV
Blast result :
Comments
ISNsp5 is 82% aa similar to ISNpu15.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Kaneko,T., Nakamura,Y., Wolk,C.P., Kuritz,T., Sasamoto,S.,
Watanabe,A., Iriguchi,M., Ishikawa,A., Kawashima,K., Kimura,T.,
Kishida,Y., Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A.,
Nakazaki,N., Shimpo,S., Sugimoto,M., Takazawa,M., Yamada,M., Yasuda,M. and Tabata,S. (2001) DNA Res. 8 (5), 205-213.
2] Kaneko,T., Nakamura,Y., Wolk,C.P., Kuritz,T., Sasamoto,S.,
Watanabe,A., Iriguchi,M., Ishikawa,A., Kawashima,K., Kimura,T.,
Kishida,Y., Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A.,
Nakazaki,N., Shimpo,S., Sugimoto,M., Takazawa,M., Yamada,M., Yasuda,M. and Tabata,S. (2001) DNA Res. 8 (5), 205-213.