ISfinder Mirror Site

ISGvi7

Family ISKra4
Group ISMich2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_005125	ND	Gloeobacter violaceus	Gloeobacter violaceus PCC 7421

DNA section

IS Length : 1220 bp

Ends

IR Length : 33/39

IRL : GGGTGCATCTCACTTATTTGATAAGCATAAATGCTTAGTCCCCTTTTGGC
IRR : GGGTGCATCTCACTTATTAGATGAGTAGAAATACTCAGTTTGTGAGTAGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATATTTCAA	AGTCGACT	TCGACATCGT	8

DNA sequence

GGGTGCATCTCACTTATTTGATAAGCATAAATGCTTAGTCCCCTTTTGGCTGTCCTGACGCACCGGTCACTGATGGGCGAAAATCGGGTATTCTCTGCCC
GCACATGACTATGGACCTCACGCCACAACAGCAACTGGCCGTCAAGGAGCATCTCGATGCTGTCGCAGCTATCCTCTACCAGAACACCCCGCCCGAGCAG
TTGACCACTCTCGAAGGCATCGAACTCGCCGTCCGCCAACACATCAACCAAGCCGTCGGACCCCAGCTTGCCCTTTTTTTGTCCAGACAATTACAGGCAC
GGTTGCTGGCTATGCCCGCCACATCACCACCGGCATCGGCCCCGTCCAAATCACCCAAAAACAAGCGCAAATCCTCCAGCTCAAGCCCGGCACCCGACTA
AGTCCGCACATGGAAAAGTGTTGCTTGCTTGTGAGTGCCAACGTCTCCTACCAGCACGCCGAGCGGGATATCGAGACCTTGACGGGTCTTCGTGTCAGCC
ATGCCACGCAGCAACGTCTGGTCCAAAGACAGCATTTTGAGTTGCCCGTCGCAAAAGAGCCTGAACTCGTCGAGGAATTGTCGGTCGATGGCGGCAAGGT
GCGCCTGCGCACCGAGCCGGGCCAAGCCTGCGAGTGGCGCGACTACAAGGCGGTGCGGCTGCACGGCATCGCTACCGCCGCGATGTATCGCGACAACGAC
GGCCTTGTGGAGTGGGTGAACCGCCAGAACCTGGCGCTCCCCGTCACTTGCCTCGGTGACGGTCACGACGGCATCTGGAACATCGTCGAGCGCATCAGCC
GTTGTGAGAGTCGGCGGGAGATTCTCGACTGGTATCACCTGAAGGAGAATCTGTACAAAGTGGGCGGGTCGTTGAATCGACTGAAGCGCGCGGAGTCTGA
TTTGTGGCGAGGAAAGGCGGAGGAGGCGATGGGCCGATTTGAGGGATGGAAGGCGGAGCAGGTAAAAAGGTTTTGCCAGTACCTGAGCAAGCACAAAGCA
CGGATTGTGAACTACGGGTATTTACAAGCAGAACAAGTCTGTTCGGTAGGTTCGGGGGCAGTGGAGTCGGCGGTCAAGCAGATAGACCGGCGGCTGAAAA
TATCGGGTGCTCAGTGGAAGGTGGAGAATGTGCCACGGGTGTTGGCCCATCGGACGGCTTACTTGAATGGCCTACTCACAAACTGAGTATTTCTACTCAT
CTAATAAGTGAGATGCACCC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
297 bp	98 aa	105	401	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTMDLTPQQQLAVKEHLDAVAAILYQNTPPEQLTTLEGIELAVRQHINQAVGPQLALFLSRQLQARLLAMPATSPPASAPSKSPKNKRKSSSSSPAPD

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1115 bp	370 aa	73	1187	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

WAKIGYSLPAHDYGPHATTATGRQGASRCCRSYPLPEHPARAVDHSRRHRTRRPPTHQPSRRTPACPFFVQTITGTVAGYARHITTGIGPVQITQKQAQI
LQLKPGTRLSPHMEKCCLLVSANVSYQHAERDIETLTGLRVSHATQQRLVQRQHFELPVAKEPELVEELSVDGGKVRLRTEPGQACEWRDYKAVRLHGIA
TAAMYRDNDGLVEWVNRQNLALPVTCLGDGHDGIWNIVERISRCESRREILDWYHLKENLYKVGGSLNRLKRAESDLWRGKAEEAMGRFEGWKAEQVKRF
CQYLSKHKARIVNYGYLQAEQVCSVGSGAVESAVKQIDRRLKISGAQWKVENVPRVLAHRTAYLNGLLTN

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1083 bp	360 aa	105	1187	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTMDLTPQQQLAVKEHLDAVAAILYQNTPPEQLTTLEGIELAVRQHINQAVGPQLALFFVQTITGTVAGYARHITTGIGPVQITQKQAQILQLKPGTRLS
PHMEKCCLLVSANVSYQHAERDIETLTGLRVSHATQQRLVQRQHFELPVAKEPELVEELSVDGGKVRLRTEPGQACEWRDYKAVRLHGIATAAMYRDNDG
LVEWVNRQNLALPVTCLGDGHDGIWNIVERISRCESRREILDWYHLKENLYKVGGSLNRLKRAESDLWRGKAEEAMGRFEGWKAEQVKRFCQYLSKHKAR
IVNYGYLQAEQVCSVGSGAVESAVKQIDRRLKISGAQWKVENVPRVLAHRTAYLNGLLTN

Blast result :

Comments

ISGvi7 is 73% aa similar to ISCysp25.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2013)
2] Nakamura,Y., Kaneko,T., Sato,S., Mimuro,M., Miyashita,H., Tsuchiya,T., Sasamoto,S., Watanabe,A., Kawashima,K., Kishida,Y., Kiyokawa,C., Kohara,M., Matsumoto,M., Matsuno,A., Nakazaki,N., Shimpo,S., Takeuchi,C., Yamada,M. and Tabata,S. (2003) DNA Res. 10 (4), 181-201.
3] Nakamura,Y., Kaneko,T., Sato,S., Mimuro,M., Miyashita,H., Tsuchiya,T., Sasamoto,S., Watanabe,A., Kawashima,K., Kishida,Y., Kiyokawa,C., Kohara,M., Matsumoto,M., Matsuno,A., Nakazaki,N., Shimpo,S., Takeuchi,C., Yamada,M. and Tabata,S.(2003) DNA Res. 10 (4), 137-145.