ISCysp25
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011880 | ND | Cyanothece sp. | Cyanothece sp. PCC 7425 plasmid pP742501 |
DNA section
IS Length : 1189 bp
Ends
IR Length : 18/21
IRL : GGGTGCATCCCAGTTATGCAATGAGTAAAAGTACTCAGAATCCTAATTTG
IRR : GGGTGCATCCCAGTCTTGTAAGTCTCAACTTGAGAACTGTCCGTTGAGAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAGCAACAA | AACGGTTCA | CGGTTCACGC | 9 |
DNA sequence
GGGTGCATCCCAGTTATGCAATGAGTAAAAGTACTCAGAATCCTAATTTGAGAACAGTTCCATTATGCTGGGGATAATCAATTGGCTATCAGGAGAGTTA
AGGTATGGACATTGATAAGCTGCAACAAATCCAAACTCATGCCCGTGCCATTGCCGCTTTACTGTACGACGAAACAGCCCCTGAACAATTGACAACACTC
GAAGGAATCGAACAAGCCGTTCGAGGACATATCCTGGAGCATGTCAGTCCAGAAATCGGTAATTTTTTATTGCAACGGCAAGTGGAACAAACCGTGGGCG
AAGTCGGCAACTCAAAAGCATCCTCGGAGCCATCCGCATCAGTGAACAGCAAGCGAAAATCCTTCAAGTGAAAGCGCGAACCCAATTGAGTCCTTACCTC
GAAAAATGTTGCTTACTGGTCAGTGCGAATGTCTCCTATGAACGGGCTGTTGAGGACGTTACCGTATTGACTGGAATGGCTGTTTCACGAGGGACACAAC
AGCGTCTAGTCCAGCGACACCCCTTTAAGTTACCAAGGGCTGAAGATAGTGTTGAGGAAATCAGTGTGGATGGTGGAAAGGTACGGATACGAACCCCAAA
AGGAGAAATTTGCCGATGGCAAGATTACAAAGCGGTTAATTTGCATGGTCATTACTGTGAAGCATTTTTGCAGGACAATGAGCAATTGATTGAGTGGGTC
AATGGACAACCTCTTGCTACTCCAGTTGTCTGTTTGGGAGATGGTCATGACGGGATCTGGAATCTGTTTAGGGAGATCGCCAGTCCCAGCCAGCGACAAG
AGATTTTAGATTGGTTTCACCTGGTTGAGAATCTCCACAAGGTAGGCGGGTCACTGCAACGATTAGCCACCGTAGAGACCCGGTTATGGAAGGGCGAGGT
CGATGCAGCCATCGAGCAATTCAAAGACTGGCAGCATGAACAGGTAGACACCTTCATTGCTTATCTCAACAAGCACCGTCACCGCATCGTCAATTACGCT
TACTACCAAGCCGAAGGAATTTCGATTGGGTCAGGCAGGATTGAATCGACGGTCAAACAGATTGGAGCACGCATGAAGCTGTCTGGGGCACAGTGGAAAG
CGGAAAACGTTCCTCAAGCTCTCCTACATCGTTGTGCTTATCTCAACGGACAGTTCTCAAGTTGAGACTTACAAGACTGGGATGCACCC
AGGTATGGACATTGATAAGCTGCAACAAATCCAAACTCATGCCCGTGCCATTGCCGCTTTACTGTACGACGAAACAGCCCCTGAACAATTGACAACACTC
GAAGGAATCGAACAAGCCGTTCGAGGACATATCCTGGAGCATGTCAGTCCAGAAATCGGTAATTTTTTATTGCAACGGCAAGTGGAACAAACCGTGGGCG
AAGTCGGCAACTCAAAAGCATCCTCGGAGCCATCCGCATCAGTGAACAGCAAGCGAAAATCCTTCAAGTGAAAGCGCGAACCCAATTGAGTCCTTACCTC
GAAAAATGTTGCTTACTGGTCAGTGCGAATGTCTCCTATGAACGGGCTGTTGAGGACGTTACCGTATTGACTGGAATGGCTGTTTCACGAGGGACACAAC
AGCGTCTAGTCCAGCGACACCCCTTTAAGTTACCAAGGGCTGAAGATAGTGTTGAGGAAATCAGTGTGGATGGTGGAAAGGTACGGATACGAACCCCAAA
AGGAGAAATTTGCCGATGGCAAGATTACAAAGCGGTTAATTTGCATGGTCATTACTGTGAAGCATTTTTGCAGGACAATGAGCAATTGATTGAGTGGGTC
AATGGACAACCTCTTGCTACTCCAGTTGTCTGTTTGGGAGATGGTCATGACGGGATCTGGAATCTGTTTAGGGAGATCGCCAGTCCCAGCCAGCGACAAG
AGATTTTAGATTGGTTTCACCTGGTTGAGAATCTCCACAAGGTAGGCGGGTCACTGCAACGATTAGCCACCGTAGAGACCCGGTTATGGAAGGGCGAGGT
CGATGCAGCCATCGAGCAATTCAAAGACTGGCAGCATGAACAGGTAGACACCTTCATTGCTTATCTCAACAAGCACCGTCACCGCATCGTCAATTACGCT
TACTACCAAGCCGAAGGAATTTCGATTGGGTCAGGCAGGATTGAATCGACGGTCAAACAGATTGGAGCACGCATGAAGCTGTCTGGGGCACAGTGGAAAG
CGGAAAACGTTCCTCAAGCTCTCCTACATCGTTGTGCTTATCTCAACGGACAGTTCTCAAGTTGAGACTTACAAGACTGGGATGCACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 105 | 371 | + | No |
Description : First part of the transposase
ORF sequence :
MDIDKLQQIQTHARAIAALLYDETAPEQLTTLEGIEQAVRGHILEHVSPEIGNFLLQRQVEQTVGEVGNSKASSEPSASVNSKRKSFK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 903 bp | 300 aa | 263 | 1165 | + | No |
Description : Second part of the transposase
ORF sequence :
FFIATASGTNRGRSRQLKSILGAIRISEQQAKILQVKARTQLSPYLEKCCLLVSANVSYERAVEDVTVLTGMAVSRGTQQRLVQRHPFKLPRAEDSVEEI
SVDGGKVRIRTPKGEICRWQDYKAVNLHGHYCEAFLQDNEQLIEWVNGQPLATPVVCLGDGHDGIWNLFREIASPSQRQEILDWFHLVENLHKVGGSLQR
LATVETRLWKGEVDAAIEQFKDWQHEQVDTFIAYLNKHRHRIVNYAYYQAEGISIGSGRIESTVKQIGARMKLSGAQWKAENVPQALLHRCAYLNGQFSS
SVDGGKVRIRTPKGEICRWQDYKAVNLHGHYCEAFLQDNEQLIEWVNGQPLATPVVCLGDGHDGIWNLFREIASPSQRQEILDWFHLVENLHKVGGSLQR
LATVETRLWKGEVDAAIEQFKDWQHEQVDTFIAYLNKHRHRIVNYAYYQAEGISIGSGRIESTVKQIGARMKLSGAQWKAENVPQALLHRCAYLNGQFSS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1061 bp | 353 aa | 105 | 1165 | + | Yes |
Chemistry : DDE
ORF sequence :
MDIDKLQQIQTHARAIAALLYDETAPEQLTTLEGIEQAVRGHILEHVSPEIGNFFIATASGTNRGRSRQLKSILGAIRISEQQAKILQVKARTQLSPYLE
KCCLLVSANVSYERAVEDVTVLTGMAVSRGTQQRLVQRHPFKLPRAEDSVEEISVDGGKVRIRTPKGEICRWQDYKAVNLHGHYCEAFLQDNEQLIEWVN
GQPLATPVVCLGDGHDGIWNLFREIASPSQRQEILDWFHLVENLHKVGGSLQRLATVETRLWKGEVDAAIEQFKDWQHEQVDTFIAYLNKHRHRIVNYAY
YQAEGISIGSGRIESTVKQIGARMKLSGAQWKAENVPQALLHRCAYLNGQFSS
KCCLLVSANVSYERAVEDVTVLTGMAVSRGTQQRLVQRHPFKLPRAEDSVEEISVDGGKVRIRTPKGEICRWQDYKAVNLHGHYCEAFLQDNEQLIEWVN
GQPLATPVVCLGDGHDGIWNLFREIASPSQRQEILDWFHLVENLHKVGGSLQRLATVETRLWKGEVDAAIEQFKDWQHEQVDTFIAYLNKHRHRIVNYAY
YQAEGISIGSGRIESTVKQIGARMKLSGAQWKAENVPQALLHRCAYLNGQFSS
Blast result :
Comments
ISCysp25 is 73% aa similar to ISMae43.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meineke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Meineke,L., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2009) Direct submission GenBank.