ISBusp5
- Family ISKra4
- Group ISAzba1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AKKD01000285 | ND | Burkholderia sp. | Burkholderia sp. BT03 PMI06 |
DNA section
IS Length : 1533 bp
Ends
IR Length : 24/26
IRL : GACGGAAGCCGTTAAAAAGGCTGGGAGACGAGCGCACCGCGGGGTATCGT
IRR : GAGGGAAGCCGTTAAAAAGACTGGGAAGTTCGTTGCCAAGGCCACGTAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCCAGTGTT | AACAGGGGGA | 0 |
DNA sequence
GACGGAAGCCGTTAAAAAGGCTGGGAGACGAGCGCACCGCGGGGTATCGTATCCCTAACGACGAGACCCAGGAGCCACCATGCGATGCACGATCGTTCTT
GAGTTCGATAACGGCGACGGGAGTGTGGTAAAGCGGGTTGAGGTCATGCGGTTGCACAGGCCCATCGACGACCAGACGCCAGGAGACGTGGGACTGTCAC
TCAGTGAAGGGAAGTCTTTGCTCAATTGTGTGCAGCAGGAGTTCGTTGTCGAGCAGATCGAACGTTTTTGCGCCTCGCATAGATCCTGTGTGGATTGCGG
CGTCCAGCGTCGTCTGCATGATAATCATTGCTCGGAGCTGAAGACCGCGCTAGGAAAGGTTTACTATTGCCGCGAACGATGGAAGGCCTGCAATTGCGGC
GCAGACGGCGCAAGATATGTTTCTCCGCTCAAGAACTACCTGCCGGAGGCAAGTACCGGCGAACTGAGATGGCTGCACGCGGAGCTTGGCGCCACGATGC
CGTACCGGCAGTCCAAGGCAGTCATGGACCTTCTGCTACCCACCTCGGGTCGCGACAATCATGTAACCATCCGCAACCACACGGTGGCCATCGGAAAATC
AATACAGCACGCACCGCCGGTGCGTCGGTGGTGCGAGACGGAAAAGCCGATTGCGGAGCTTGGCATTGACGTCGGCTATGTCCGTCGCGCACGTTGCAAT
CGCAAGGGTTCCGGCAAGGAAAATCGCGCAGAAGCCGGGAACCGGCCGTCGTCGATCGCAGTCGTCGTTGCTGCTTTGGGCCAAGCCGGTAAGCAGCCAC
GAGTGTGGGCTTCCGCCATGCCGCGCACCAGGCGACTGAATCAAGCAATGACACGATTCCTCGAGGACAGCGGATACGGGGATCCTAATGAAGTCGTTGT
CCTGACTGATGGAGCTCGGGACCTGGCCGGCGTCGCTAATGATCTCCCATACGATTACGAGTGGATTCTCGATTGGGCACACATCGGGCGCATGTTGCGA
CGCGTTGAGCAGGCTATCGCGCCTCTGGCATATGGGCGACTCACGGACAGTGGATCAGCGTTTGAACTGTGGGATCTGTTTGTCCGCTTCCGCCACTACG
TTTGGGTCGGACCTACTGCCGCCTGGCAGCAGTTTGCAGCTAGACTTGCTCACTTGCTTGATCTACGTGAGACACGAGATCCAGCAGTGAGCAGCCAAGT
ATGGAAAGCGAGCTATAGCCTCTCGAATGTAGTCACGTATCTCGACAGCAACGCCGAGTCGCTGATCGACTATCGCACATGGCAACGTGCGGGAGGACGA
ATCTCCACCGGCTTCGTCGAATCGTCAATTAATCGAATCGTTGGCCGTCGAATGTGCAAGAGCCAGCATATGCGCTGGAGTCGCGTGGGGGCACACAGTG
TCGTGCAATTGCGCGTCGCGTTGTTGAACCAGGAATTCCACGAACTCGCGCGACGACAGTTTCCGTGGATTGGACAGCGACGTGTTACGTGGCCTTGGCA
ACGAACTTCCCAGTCTTTTTAACGGCTTCCCTC
GAGTTCGATAACGGCGACGGGAGTGTGGTAAAGCGGGTTGAGGTCATGCGGTTGCACAGGCCCATCGACGACCAGACGCCAGGAGACGTGGGACTGTCAC
TCAGTGAAGGGAAGTCTTTGCTCAATTGTGTGCAGCAGGAGTTCGTTGTCGAGCAGATCGAACGTTTTTGCGCCTCGCATAGATCCTGTGTGGATTGCGG
CGTCCAGCGTCGTCTGCATGATAATCATTGCTCGGAGCTGAAGACCGCGCTAGGAAAGGTTTACTATTGCCGCGAACGATGGAAGGCCTGCAATTGCGGC
GCAGACGGCGCAAGATATGTTTCTCCGCTCAAGAACTACCTGCCGGAGGCAAGTACCGGCGAACTGAGATGGCTGCACGCGGAGCTTGGCGCCACGATGC
CGTACCGGCAGTCCAAGGCAGTCATGGACCTTCTGCTACCCACCTCGGGTCGCGACAATCATGTAACCATCCGCAACCACACGGTGGCCATCGGAAAATC
AATACAGCACGCACCGCCGGTGCGTCGGTGGTGCGAGACGGAAAAGCCGATTGCGGAGCTTGGCATTGACGTCGGCTATGTCCGTCGCGCACGTTGCAAT
CGCAAGGGTTCCGGCAAGGAAAATCGCGCAGAAGCCGGGAACCGGCCGTCGTCGATCGCAGTCGTCGTTGCTGCTTTGGGCCAAGCCGGTAAGCAGCCAC
GAGTGTGGGCTTCCGCCATGCCGCGCACCAGGCGACTGAATCAAGCAATGACACGATTCCTCGAGGACAGCGGATACGGGGATCCTAATGAAGTCGTTGT
CCTGACTGATGGAGCTCGGGACCTGGCCGGCGTCGCTAATGATCTCCCATACGATTACGAGTGGATTCTCGATTGGGCACACATCGGGCGCATGTTGCGA
CGCGTTGAGCAGGCTATCGCGCCTCTGGCATATGGGCGACTCACGGACAGTGGATCAGCGTTTGAACTGTGGGATCTGTTTGTCCGCTTCCGCCACTACG
TTTGGGTCGGACCTACTGCCGCCTGGCAGCAGTTTGCAGCTAGACTTGCTCACTTGCTTGATCTACGTGAGACACGAGATCCAGCAGTGAGCAGCCAAGT
ATGGAAAGCGAGCTATAGCCTCTCGAATGTAGTCACGTATCTCGACAGCAACGCCGAGTCGCTGATCGACTATCGCACATGGCAACGTGCGGGAGGACGA
ATCTCCACCGGCTTCGTCGAATCGTCAATTAATCGAATCGTTGGCCGTCGAATGTGCAAGAGCCAGCATATGCGCTGGAGTCGCGTGGGGGCACACAGTG
TCGTGCAATTGCGCGTCGCGTTGTTGAACCAGGAATTCCACGAACTCGCGCGACGACAGTTTCCGTGGATTGGACAGCGACGTGTTACGTGGCCTTGGCA
ACGAACTTCCCAGTCTTTTTAACGGCTTCCCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1443 bp | 480 aa | 80 | 1522 | + | No |
Chemistry : DDE
ORF sequence :
MRCTIVLEFDNGDGSVVKRVEVMRLHRPIDDQTPGDVGLSLSEGKSLLNCVQQEFVVEQIERFCASHRSCVDCGVQRRLHDNHCSELKTALGKVYYCRER
WKACNCGADGARYVSPLKNYLPEASTGELRWLHAELGATMPYRQSKAVMDLLLPTSGRDNHVTIRNHTVAIGKSIQHAPPVRRWCETEKPIAELGIDVGY
VRRARCNRKGSGKENRAEAGNRPSSIAVVVAALGQAGKQPRVWASAMPRTRRLNQAMTRFLEDSGYGDPNEVVVLTDGARDLAGVANDLPYDYEWILDWA
HIGRMLRRVEQAIAPLAYGRLTDSGSAFELWDLFVRFRHYVWVGPTAAWQQFAARLAHLLDLRETRDPAVSSQVWKASYSLSNVVTYLDSNAESLIDYRT
WQRAGGRISTGFVESSINRIVGRRMCKSQHMRWSRVGAHSVVQLRVALLNQEFHELARRQFPWIGQRRVTWPWQRTSQSF
WKACNCGADGARYVSPLKNYLPEASTGELRWLHAELGATMPYRQSKAVMDLLLPTSGRDNHVTIRNHTVAIGKSIQHAPPVRRWCETEKPIAELGIDVGY
VRRARCNRKGSGKENRAEAGNRPSSIAVVVAALGQAGKQPRVWASAMPRTRRLNQAMTRFLEDSGYGDPNEVVVLTDGARDLAGVANDLPYDYEWILDWA
HIGRMLRRVEQAIAPLAYGRLTDSGSAFELWDLFVRFRHYVWVGPTAAWQQFAARLAHLLDLRETRDPAVSSQVWKASYSLSNVVTYLDSNAESLIDYRT
WQRAGGRISTGFVESSINRIVGRRMCKSQHMRWSRVGAHSVVQLRVALLNQEFHELARRQFPWIGQRRVTWPWQRTSQSF
Blast result :
Comments
ISBusp5 is 45% aa similar to ISSysp7.
References
1] ISfinder annotation (2013)
2] Brown,S.D., Utturkar,S.M., Klingeman,D.M., Johnson,C.M., Martin,S.L., Land,M.L., Lu,T.-Y.S., Schadt,C.W., Pelletier,D.A. and Doktycz,M.J. (2012) Direct submission GenBank.
2] Brown,S.D., Utturkar,S.M., Klingeman,D.M., Johnson,C.M., Martin,S.L., Land,M.L., Lu,T.-Y.S., Schadt,C.W., Pelletier,D.A. and Doktycz,M.J. (2012) Direct submission GenBank.