IS5Sa2

  • Family IS5
  • Group IS1031
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB001339 ND Synechocystis sp.
Synechocystis sp. PCC6803
DNA section
IS Length : 908 bp

Ends


IR Length : 16/17

IRL : GAGCTCATTTATAAAGTCTTGTAATGGATAGCAAGTAGGAGAAGAAAAAA
IRR : GAGCCCATTTATAAAGTGGTAGAAGTAGCAGAAAGACGACGCAACATAAT

Insertion site


Left flankDirect repeatRight flankDR Length
ACTCTCAACCATAGCAGCCCGCA3

DNA sequence

GAGCTCATTTATAAAGTCTTGTAATGGATAGCAAGTAGGAGAAGAAAAAAAGGAAAACTAAGAGTAAGAGCAGAAGGAGACAAAGAGCATGAAAAGTCTG
ACAGCCTATGAAACAGACCTGAGCGATGCAGAATGGGCAATACTTGAACCAATGATTCCTCCCGCAAAGCCCGGAGGACACCCACGAACCACAGATATGC
GAGCGGTTTGTAATGGTATTTACTACCAACTAAAAACGGGTTGTCAATGGGCAATGTTACCCCATGACTTTCCTCCTAGTAGCACGGTCTATAGCTATTA
CCGTAAATGGCAACGTCATGGCGTGTGGGAAAGGCTCAATCTAAGGCTGTTGGAGCAATGCCGCCTCCAGAGTGGCAAGAGCCTTGAGCCCAGCGTGATA
GCGGCAGATAGTCAATCGGTAAAAACCACCGAAAAAAGGGGGAAGTCTACGGTTTTGACGGAGGTAAGAAGGTGAAAGGACGAAAGCGTCATCTAATCGT
CGATAGTCTGGGCTTAGTCTTAAAAGTGATAGTGACCGAAGCAAATGCAAGTGAACGCATCGTCACAGCTTATGCTCTGATGAGTTTGCTTGAAGAAGGA
ACTCAACTCCTCGAGTCTGTCAAGAGCATGTTAGTAGACCAGGGTTATCGAGGTGATACCTTTGGGTTAGCCATCTGGCTCTTAATTCAAGCCAAAGTCC
AGGTGATTAGTCGTTCAGGGAAGTCTTTTGAGGTCTTACCAAAAAGATGGATTGTCGAGAGGACGTTCGGTTGGTTGAACTGGTATCGTCGTTTGAGTAA
GGATTATGAGCATCTACCAGAAATGAGCGAAGCGGCTATTTATGCCGTGATGACTCGCATTATGTTGCGTCGTCTTTCTGCTACTTCTACCACTTTATAA
ATGGGCTC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
387 bp128 aa89475+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKSLTAYETDLSDAEWAILEPMIPPAKPGGHPRTTDMRAVCNGIYYQLKTGCQWAMLPHDFPPSSTVYSYYRKWQRHGVWERLNLRLLEQCRLQSGKSLE
PSVIAADSQSVKTTEKRGKSTVLTEVRR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
489 bp162 aa412900+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SIGKNHRKKGEVYGFDGGKKVKGRKRHLIVDSLGLVLKVIVTEANASERIVTAYALMSLLEEGTQLLESVKSMLVDQGYRGDTFGLAIWLLIQAKVQVIS
RSGKSFEVLPKRWIVERTFGWLNWYRRLSKDYEHLPEMSEAAIYAVMTRIMLRRLSATSTTL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
812 bp270 aa89900+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSLTAYETDLSDAEWAILEPMIPPAKPGGHPRTTDMRAVCNGIYYQLKTGCQWAMLPHDFPPSSTVYSYYRKWQRHGVWERLNLRLLEQCRLQSGKSLE
PSVIAADSQSVKTTEKKGEVYGFDGGKKVKGRKRHLIVDSLGLVLKVIVTEANASERIVTAYALMSLLEEGTQLLESVKSMLVDQGYRGDTFGLAIWLLI
QAKVQVISRSGKSFEVLPKRWIVERTFGWLNWYRRLSKDYEHLPEMSEAAIYAVMTRIMLRRLSATSTTL

 

Blast result :
Comments
See Synechocystis sp. Genome (cf. genome section in ISDatabase).
IS5Sa2 is 83% aa similar to ISAva7. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1]Kaneko,T., Sato,S., Kotani,H., Tanaka,A., Asamizu,E., Nakamura,Y., Miyajima,N., Hirosawa,M., Sugiura,M., Sasamoto,S., Kimura,T., Hosouchi,T., Matsuno,A., Muraki,A., Nakazaki,N., Naruo,K., Okumura,S., Shimpo,S., Takeuchi,C., Wada,T., Watanabe,A., Yamada,M., Yasuda,M. and Tabata,S.(1996) DNA Res. 3 (3), 109-136