ISfinder Mirror Site

ISSysp7

Family ISKra4
Group ISAzba1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010474	ND	Synechococcus sp.	Synechococcus sp. PCC 7002 plasmid pAQ6 Synechococcus sp. PCC 7002 plasmid pAQ7

DNA section

IS Length : 2833 bp

Ends

IR Length : 23

IRL : GAGAGTGATTAAAAACGGGGGAAAGTATACTCAGAGTGGATCACAGGGAT
IRR : GAGAGTGATTAAAAACGGGGGAATCAAGCAGCATTAGGCACTCCAGTCTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACTCCCAGAG		AACCAAGACC	0
CCTTTATTAT		CTCCATAATT	0

DNA sequence

GAGAGTGATTAAAAACGGGGGAAAGTATACTCAGAGTGGATCACAGGGATCTAAATTCTCATCAACTTTTTACCGTTTCCGCTTCCTTCAAATAGCGATA
GATAGTTGCCTTAGAAAGGCCGTAATCTTCCATCAACGTTTTGATTAATACCCCTTGTGAGCGTCTTTTACGTAGTTCTTGGCATTGACTTGGCGTGAGC
TGCTTTTTACGGCCAAACTGAACCCCACGAGCTTTCGCATTTTGGATGCCATCCATTTGACGTTCCGCACGTATTTCCGTCTCAAATTGGGAGATCGCCC
CCAACATATTGAATAAAAGCCGTCCGGTCGCATCTCCAGTATCAATACTCTGTTCAAGCACTTGAAGATTCACCTGCTTAGATTCCAATTGTTTCGCAAT
TTCGCACAGGTGCAGGGTTGAGCGAGCCAGGCGATCTAAACGAGTCACAACTAATGTGTCGCCTTCTCTGACATATTCAAGACAGGCTTTAAGCCTAGGG
CGTTTGTACGAGACACCACTGCACTTTTCTTTGAAAATCTTGTCGCAATGCTTGAGCTTCTCGATTTGAACTTCCAGACTCTGCCCAACAGAACTGACTC
GCGCGTAGCCAACCATCGCCATGATGACTCACCGTAATGTTTTATAACGTCTTAGACACGATGATAAAGCCATAATGAGACATTAAATAAAACACTGTTT
TGCACATTCTTGCAAAGTACACTTTATAAGACTATTGAGTCTATTCGTTGATGTAGTGGTATGGATAGTGGCTCAGGCTCACATGCATTTCAGATCAAAG
CTGTTATTGCCGGTGTCAGCCCAATGATCTGGCGTCGGTTCCTAGTGAGAGGCGACACAACGATTGCTCAACTACATTTTATTCTACAGATCGCTTTTGG
CTGGAGCGACACATATTTACATCGCTTCGTCATCCACGGCAACTCCTACGGCATCCATCATATTGGTGGGGAATGCTTCAGCGATGATCCCCATTCTGTA
ACTCTCTTAAGTCTGGGTTTGCGGGTGCGAGAACGGTTTCGCTACGAGTACAACTATTACGATAACTGGCAGGTACAACTGCGAGTTGAAAAGATAACTG
AACTTGAGCCCGAAAAAACTTACCCGATTTGTATTGAAGGTAAACGGAATGGCCCCATTGAAGATTGTGGAGGGGCTTGGGCTTTCCAGGAACTTAGACA
GGGATTCTCTACAGGAATGGTACTCCACCGCATGGCTGAAATTCTTTTCCAAGGAGATGATGAGCTTAAGCCACATCTGAGTGAACTTCGGCAACTGAGT
TATTGGCTGTTACTAGACAACCTTGAGTTACCAAAACTCAATCATCGCTTACAGCAAAATCCCTTGAGTGACGACCCAGCTTTTCGAGAGGACATTATTT
TCTAGGCTTATGAATATCAAAATCAGCTTAAGGATTGAAGCCGATGATGGCAGCATAAAAATCATTGAGGATGTCGCTCAATTGGAGCGAGGCCCATTGA
CTCCTACTAATCTAGGCTTGACTTTGGCCGAATCAAAACAGATTTTGCAAGGGATCCAACAAGAAATGGTTTCGTCACAGGTCAGTCAATATGTAGAGCA
ACAGGCGTTTTGTCCAGACTGTGGTCAACCGCGTAAATGTAAAGGGAAACATAAGCTAATTTATCGTTCTGTATTTGGGAAGCTGACATTAACTAGTCCT
CGATTCTTTCATTGTCCCTGCCAGTCCCAAGCAACAAAAAGCTTTAGCCCATTAGCCTTACTCCTCACTGAACGGCAGTCTCCTGAATACCTTTACCTGC
AGACAAAATTTGCCTCCCTGGTTTCCTATGGTCTGAGTGTCCAGTTACTTGATGAGGTGTTGCCATTGAACGGCACCCTTAATCCTTCAACCGTGAGATA
TCACCTGCATCAAATGGGGCAAAAGCTCGATGATGAATTGGGAGAAGAACAAATTATGTATGTAGAAGGTAGCCCTCGCCAATGGGAGCAACTCCCTCGA
CCTGAGCTTCCCCTCAATGTAGGAATTGATGGTGCCTATATCCATGCTCACTGTTCTAAAGGGAGTAAACAACAGCGCCATTTTGAACTGATTGTGGGCA
AGAGTATTCCAGATGAAGGACCCAGCAAAAGCTTTGGTTTTGTCCAGACCTATGACACCAAACCAAAGCGACGGTTATTTGAACTCCTAAAATCCCAAGG
AATGCAAATGAATCAGCAGGTTACCTTCTTTTCTGATGGAGGCGATACAGTCCGAGACTTGCAGCGATTTCTCAATCCTCAAGCGGAATATCTTCTGGAC
TGGTTTCATGTCACCATGCGGATCACTCAGCTCAGTCAATATCTCAGGGGGTTGAAGCACTATGATTTAGACAATGGAAAGTCTATGGTAGAGAGGCTAA
AGAGTGTCAAATGGTATCTCTGGCATGGGAATGTCTTCAGGGCATTACAAGAGCTGGAATGGCTGGAGGAGGATGCTGACTTTCTCGAAAGTAATTATCC
GAAACTCAAAAAAATGCGGCGGGCTATTGCTGAGTTCCACACGTATATTCAGAACAATGGCCATTTTATTCCCAACTATGGAGAACGTTACCGCTGTGGG
GAGCGCATCTCAACGGGATTTGTGGAATCTACAGTCAATCAAGTCGTGGCTAAACGAATGGAGAAGAAGCAGCAAATGCGATGGACTCCGAAGGGTGCTC
ATCTATTACTACAGGTGAGAACTAAAGTCCTAAATCAAGACTGGAAAGAAGTGGTTGACCGATGGTATTCTCCGCCACTGAAGGAGACTGGAGTGCCTAA
TGCTGCTTGATTCCCCCGTTTTTAATCACTCTC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
561 bp	186 aa	622	62	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAMVGYARVSSVGQSLEVQIEKLKHCDKIFKEKCSGVSYKRPRLKACLEYVREGDTLVVTRLDRLARSTLHLCEIAKQLESKQVNLQVLEQSIDTGDATG
RLLFNMLGAISQFETEIRAERQMDGIQNAKARGVQFGRKKQLTPSQCQELRKRRSQGVLIKTLMEDYGLSKATIYRYLKEAETVKS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
645 bp	214 aa	761	1405	+	No

ORF function : Passenger Gene
Annotation : plasmid pRiA4b ORF-3 family proteinDescription :

ORF sequence :

MDSGSGSHAFQIKAVIAGVSPMIWRRFLVRGDTTIAQLHFILQIAFGWSDTYLHRFVIHGNSYGIHHIGGECFSDDPHSVTLLSLGLRVRERFRYEYNYY
DNWQVQLRVEKITELEPEKTYPICIEGKRNGPIEDCGGAWAFQELRQGFSTGMVLHRMAEILFQGDDELKPHLSELRQLSYWLLLDNLELPKLNHRLQQN
PLSDDPAFREDIIF

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1401 bp	466 aa	1410	2810	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNIKISLRIEADDGSIKIIEDVAQLERGPLTPTNLGLTLAESKQILQGIQQEMVSSQVSQYVEQQAFCPDCGQPRKCKGKHKLIYRSVFGKLTLTSPRFF
HCPCQSQATKSFSPLALLLTERQSPEYLYLQTKFASLVSYGLSVQLLDEVLPLNGTLNPSTVRYHLHQMGQKLDDELGEEQIMYVEGSPRQWEQLPRPEL
PLNVGIDGAYIHAHCSKGSKQQRHFELIVGKSIPDEGPSKSFGFVQTYDTKPKRRLFELLKSQGMQMNQQVTFFSDGGDTVRDLQRFLNPQAEYLLDWFH
VTMRITQLSQYLRGLKHYDLDNGKSMVERLKSVKWYLWHGNVFRALQELEWLEEDADFLESNYPKLKKMRRAIAEFHTYIQNNGHFIPNYGERYRCGERI
STGFVESTVNQVVAKRMEKKQQMRWTPKGAHLLLQVRTKVLNQDWKEVVDRWYSPPLKETGVPNAA

Blast result :

Comments

ISSysp7 is 65% aa similar to ISAzs26.
ORFA and ORFB are passenger gene respectively annotated as hypothetical protein and resolvase/recombinase.

References

1] ISfinder annotation (2013)
2] Li,T., Zhao,J., Zhao,C., Liu,Z., Zhao,F., Marquardt,J., Nomura,C.T., Persson,S., Detter,J.Chris., Richardson,P.M., Lanz,C., Schuster,S.C., Wang,J., Li,S., Huang,X., Cai,T., Yu,Z., Luo,J., Zhao,J. and Bryant,D.A. (2008) Direct submission GenBank.