ISHhy1
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_015510 | ND | Haliscomenobacter hydrossis | Haliscomenobacter hydrossis DSM 1100 |
DNA section
IS Length : 1356 bp
Ends
IR Length : 23/25
IRL : GGGTGCGGCTCTAGTTTGATGTAGAGAAAGAATAAAAATAGTATTTTGTG
IRR : GGGTGCGGCTCTAATTTGATGTACAATTAGGCTCTAATTTGATCGTAAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCAACTAAA | GTACCAAC | TCCTCCCCAA | 8 |
| GAATGTGTAA | AAGACAAG | TTGAATTAGC | 8 |
| ATCAATTGTA | GAATGCGA | GGGAGATTAT | 8 |
| GTTGAACCAT | TCCCTATA | GCCGCCAAGA | 8 |
DNA sequence
GGGTGCGGCTCTAGTTTGATGTAGAGAAAGAATAAAAATAGTATTTTGTGATCCAAGAACAAAAGGCTGATGGAAAAGTCATACAAAATAGAATCAGACG
GGAGTATAAGCATCACCTTCAATTTTAAACCAGAAGGGAGCATGCTGGAGCAAGAAGAGCAGATAGCGGCGGCGTTATCAGAGGCGGGTCGTCTAGCGAG
CGAACTATCGCTGAAAAGCTTCGATACGGGAGGAGAAAGCATTATCGTAAACAACGAAAAGCTGAGTAGTCGGGGTGAGGAAAAAAAACTACGAAACCGC
CTGGGGTCGAGTAAGCATTAGTCGCCATGTTTACCAGCGGAGTCGGGGAGGAAAAATATACGTTCCATTAGAAGTAGGGGCAAGGGTAGTTGGCGGAAAT
TGCACGCCGTATCTGAGTAAAATGGTGAGTTGGAAATACGCTCAATTAGCCGCATCGCGGGTCTGCGAGGACATGGCAATGCACCATCATCGAAGCATTA
GTCGGAAATTGGTACAAAAAATAGGGAGTGAGGTAGGTCTGATCGCCTGGGACAAAGAGATGGAGTGGTCATATGATTTACCTGAGCTAAAAGAGGTAGT
AAAGTGTGTGGCGATCAGCCGAGATGGGACGACGACACCGATTATTGGTCAGGGGTATCGAGAAACGATGTGCGGCACGATTAGTTTGTACAACAAAACT
GGAGATCGCCTTCATACCATTTATAAAGCGTGTGCCCCCGAAAAGGGTAAAGAAGGCTTTGATCGGGTCATGAACAAAGAGGTAGAGGAAATTAAAGCCT
TATTCCCTTCAGCCAAGTATGTTGGCTTGGCCGATGGAGCAGCCAATAACTGGACTTACCTCAATGGATGCACAACTGAGCAGGTTCTGGATTTTTACCA
TGCCACTGAGCACCTCAGCGAGGTGAGTGTGGCGATGGAACCAAACGAGGAACGACGTAAGAAGTGGCTGGATGAAGCCTGTCATGACTTGAAACACCGA
CCTAAAGCTGCGGTTTTTTTATTCAGAGAGCTCCAAGCCAAACAAAAAGAATATGGTGAGCATCCGCCGCAGGTATTATTGGACAACCTCACCTACTTGA
GTAACAACCTTAAACGAATGGACTACTGCCGATTCCTCAAAAACGGCTTCCCGATTGGATCAGGAGTTACTGAGGCAGCCTGCAAAGTCCTGGCCAAACA
ACGCTTGAGCGGTTCAGGTATGCGATGGCATCTCCATAAAGTACAAGATATGCTGTTGATTAGAGAACTCGTCTGTACAAGGGGGCGCTGGGAACAGTTC
TGGGCCTTTTACGATCAAATTAGAGCCTAATTGTACATCAAATTAGAGCCGCACCC
GGAGTATAAGCATCACCTTCAATTTTAAACCAGAAGGGAGCATGCTGGAGCAAGAAGAGCAGATAGCGGCGGCGTTATCAGAGGCGGGTCGTCTAGCGAG
CGAACTATCGCTGAAAAGCTTCGATACGGGAGGAGAAAGCATTATCGTAAACAACGAAAAGCTGAGTAGTCGGGGTGAGGAAAAAAAACTACGAAACCGC
CTGGGGTCGAGTAAGCATTAGTCGCCATGTTTACCAGCGGAGTCGGGGAGGAAAAATATACGTTCCATTAGAAGTAGGGGCAAGGGTAGTTGGCGGAAAT
TGCACGCCGTATCTGAGTAAAATGGTGAGTTGGAAATACGCTCAATTAGCCGCATCGCGGGTCTGCGAGGACATGGCAATGCACCATCATCGAAGCATTA
GTCGGAAATTGGTACAAAAAATAGGGAGTGAGGTAGGTCTGATCGCCTGGGACAAAGAGATGGAGTGGTCATATGATTTACCTGAGCTAAAAGAGGTAGT
AAAGTGTGTGGCGATCAGCCGAGATGGGACGACGACACCGATTATTGGTCAGGGGTATCGAGAAACGATGTGCGGCACGATTAGTTTGTACAACAAAACT
GGAGATCGCCTTCATACCATTTATAAAGCGTGTGCCCCCGAAAAGGGTAAAGAAGGCTTTGATCGGGTCATGAACAAAGAGGTAGAGGAAATTAAAGCCT
TATTCCCTTCAGCCAAGTATGTTGGCTTGGCCGATGGAGCAGCCAATAACTGGACTTACCTCAATGGATGCACAACTGAGCAGGTTCTGGATTTTTACCA
TGCCACTGAGCACCTCAGCGAGGTGAGTGTGGCGATGGAACCAAACGAGGAACGACGTAAGAAGTGGCTGGATGAAGCCTGTCATGACTTGAAACACCGA
CCTAAAGCTGCGGTTTTTTTATTCAGAGAGCTCCAAGCCAAACAAAAAGAATATGGTGAGCATCCGCCGCAGGTATTATTGGACAACCTCACCTACTTGA
GTAACAACCTTAAACGAATGGACTACTGCCGATTCCTCAAAAACGGCTTCCCGATTGGATCAGGAGTTACTGAGGCAGCCTGCAAAGTCCTGGCCAAACA
ACGCTTGAGCGGTTCAGGTATGCGATGGCATCTCCATAAAGTACAAGATATGCTGTTGATTAGAGAACTCGTCTGTACAAGGGGGCGCTGGGAACAGTTC
TGGGCCTTTTACGATCAAATTAGAGCCTAATTGTACATCAAATTAGAGCCGCACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 252 bp | 83 aa | 70 | 321 | + | No |
Description : First part of the transposase
ORF sequence :
MEKSYKIESDGSISITFNFKPEGSMLEQEEQIAAALSEAGRLASELSLKSFDTGGESIIVNNEKLSSRGEEKKLRNRLGSSKH
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1065 bp | 354 aa | 266 | 1330 | + | No |
Description : Second part of the transposase
ORF sequence :
VVGVRKKNYETAWGRVSISRHVYQRSRGGKIYVPLEVGARVVGGNCTPYLSKMVSWKYAQLAASRVCEDMAMHHHRSISRKLVQKIGSEVGLIAWDKEME
WSYDLPELKEVVKCVAISRDGTTTPIIGQGYRETMCGTISLYNKTGDRLHTIYKACAPEKGKEGFDRVMNKEVEEIKALFPSAKYVGLADGAANNWTYLN
GCTTEQVLDFYHATEHLSEVSVAMEPNEERRKKWLDEACHDLKHRPKAAVFLFRELQAKQKEYGEHPPQVLLDNLTYLSNNLKRMDYCRFLKNGFPIGSG
VTEAACKVLAKQRLSGSGMRWHLHKVQDMLLIRELVCTRGRWEQFWAFYDQIRA
WSYDLPELKEVVKCVAISRDGTTTPIIGQGYRETMCGTISLYNKTGDRLHTIYKACAPEKGKEGFDRVMNKEVEEIKALFPSAKYVGLADGAANNWTYLN
GCTTEQVLDFYHATEHLSEVSVAMEPNEERRKKWLDEACHDLKHRPKAAVFLFRELQAKQKEYGEHPPQVLLDNLTYLSNNLKRMDYCRFLKNGFPIGSG
VTEAACKVLAKQRLSGSGMRWHLHKVQDMLLIRELVCTRGRWEQFWAFYDQIRA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1261 bp | 420 aa | 70 | 1330 | + | Yes |
Chemistry : DDE
ORF sequence :
MEKSYKIESDGSISITFNFKPEGSMLEQEEQIAAALSEAGRLASELSLKSFDTGGESIIVNNEKLSSRGEEKKNYETAWGRVSISRHVYQRSRGGKIYVP
LEVGARVVGGNCTPYLSKMVSWKYAQLAASRVCEDMAMHHHRSISRKLVQKIGSEVGLIAWDKEMEWSYDLPELKEVVKCVAISRDGTTTPIIGQGYRET
MCGTISLYNKTGDRLHTIYKACAPEKGKEGFDRVMNKEVEEIKALFPSAKYVGLADGAANNWTYLNGCTTEQVLDFYHATEHLSEVSVAMEPNEERRKKW
LDEACHDLKHRPKAAVFLFRELQAKQKEYGEHPPQVLLDNLTYLSNNLKRMDYCRFLKNGFPIGSGVTEAACKVLAKQRLSGSGMRWHLHKVQDMLLIRE
LVCTRGRWEQFWAFYDQIRA
LEVGARVVGGNCTPYLSKMVSWKYAQLAASRVCEDMAMHHHRSISRKLVQKIGSEVGLIAWDKEMEWSYDLPELKEVVKCVAISRDGTTTPIIGQGYRET
MCGTISLYNKTGDRLHTIYKACAPEKGKEGFDRVMNKEVEEIKALFPSAKYVGLADGAANNWTYLNGCTTEQVLDFYHATEHLSEVSVAMEPNEERRKKW
LDEACHDLKHRPKAAVFLFRELQAKQKEYGEHPPQVLLDNLTYLSNNLKRMDYCRFLKNGFPIGSGVTEAACKVLAKQRLSGSGMRWHLHKVQDMLLIRE
LVCTRGRWEQFWAFYDQIRA
Blast result :
Comments
ISHhy1 is 41% aa similar to ISDere1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References