ISDesp4
- Family ISKra4
- Group ISKra4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FR695879.1 | ND | Desulfobacterium sp. | Desulfobacterium sp. (Uncultured) |
DNA section
IS Length : 1849 bp
Ends
IR Length : 21/25
IRL : GGGTCTGCGACATAAATATGGGTAAAAACTTGACAAGGCGAGATATTTCG
IRR : GGGTGTGCGACAAATTTATGGGTAACGATTTCAGATTGCGGCCCGATGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCGAAATTT | AAATCATAAA | 0 | |
| CAAATACCAG | ATTCGATAAC | 0 | |
| GATTCGATAA | AAATCATAAA | 0 | |
| TTTTTGTGAA | AAATACCAGA | 0 | |
| TACAGAAACA | GCCTGAAGTT | 0 |
DNA sequence
GGGTCTGCGACATAAATATGGGTAAAAACTTGACAAGGCGAGATATTTCGCCTATAGTCTGATTGCTTGTTAAGCATTCAGACCATAGGAGGAAGCCGAT
GGAAGAAACTATTGAAAGCTTGGAGATAGAGCGGGAACATTTATATAGGCAATTGCAAGAAACCGGAGATTTCCGGAGGGGAATCATTTCAGTTGTATAC
CGTAAATGTGGGAAAAAAAACTGCGCATGCGCTAAAGAGGGGCATCCAGGGCATGGGCCGCAACATTTATGGAACACAACAATCAAAGGGAAAAGCTACG
CGAAGAGCGTAAAACTTGGTCCGGAGTTGCAAAAGTACCTGGAGGAGATTGCGAACCATCAACGCTATGTAAAGCTGTGTGAAGAGATTGTTTTGGTAAA
CGAGCGTATCTGCGACCTTCGACCAGTGCCCGAAGTGAAAGATGATCAGGAGTTGGCCGAACTTAAAAAAAAATTGCAGAGTCTGTTCATGAAGAAGTAC
AAAATGAGATAGGTCGCATTGTTGGCCGGTATGTTTCAGAAAAAACGACCAACGGCAATTTTGACTTTGAAGCGTTTGAAATGAGCATCCGTTCGTCAAT
GCACCGCATTGGCGGCAGCATACTTGGAATACTCGTAAACGCAGATGGCGGAGGTTACCGGGGTAAAACCATTACCTGCGGGCAAGGGCATCAGTATGAG
TTTATCGATTACCGGAACAAGGAACTGCTCACTGTGTTGGGCCCGGTGGCCGTTAAGCGGGCATACTATTATGATCGAAAATGTCATGTTGGTTTTTGCC
CGAAAGACCGTGATCTGGACATAGGGACATCTTACAGTTGTGGGGTACGCCGGATGATGAGCAAGGTTGGCGCCTACCGGCCATTCGGCCTGGGTCATCA
AGACTTGTATGAGCTGGCGGATATCCGTGTCAACACAAAAGCAGTAGAGCGTGTATCACAGACAGTAGGTGCTCAGGTGGAAGCGTTTCATGCAGCCGAG
GCCAGGACGTCGTTGTCGGATAAAATTGTACCGATCAAACCTGTTCCACGGATGTATGTGTGCATGGATGGCACCGGAGTTCCGGTAGTTAAGAAAGAGA
CTGCCGGCAGGCAAGGCAAATGTGAAGACGGTCAGGCAAAGACCAGGGAAGTCAAGCTGGGTTGCATTTTTACCCAGACAGAAGTTGACCGGAAAGGCCG
TCCGGTGCGAGACGATGGGACCACCAGCTACACCGGTGCAATCGAATCGGCTGAGGTGTTTGGACAGCGGATATATCAAGAGGCACTTCGGCGCGGCATG
CGCCGGGCAAAGGAGGTTTGTGTGCTTGGGGACGGGGCCATCTGGATATGGAATATAGCGGATGAACAATTTTATGGTGCTATCCAGATTGTCGATCTGT
TCCATGCGCGTGAACATTACTGGAACGTCGGTAAGGCATATTTCAGTCAGAATAAGGACAAACTGCATCTGTGGACGGAAGAGCGTCGGAAAGAATTGGA
TGATGGAAGGGTCGAAGATGTAATGAATGCCATCAAGGAATGCTCATCTCAATCGGGATGCGACAAATCAATATATGAACGAGAGATCGGTTATTTTGAA
AAAAACAAGGAAAGGATGCGGTACGCCCATTTTAGGAAGCGGGGTCTTTTTATAGGTTCCGGCGTTCTGGAGGCAGGCTGTCGAACCGTGGTTGGACAGC
GGCTGAAACAGTCGGGTATGCACTGGACGGTTAGTGGCGCAAATAGTATAATAGCTCTGCGCTGTAGTATTCTAAGCAACCGTTGGGAAGATTTTTGGGA
ACATCGGGCCGCAATCTGAAATCGTTACCCATAAATTTGTCGCACACCC
GGAAGAAACTATTGAAAGCTTGGAGATAGAGCGGGAACATTTATATAGGCAATTGCAAGAAACCGGAGATTTCCGGAGGGGAATCATTTCAGTTGTATAC
CGTAAATGTGGGAAAAAAAACTGCGCATGCGCTAAAGAGGGGCATCCAGGGCATGGGCCGCAACATTTATGGAACACAACAATCAAAGGGAAAAGCTACG
CGAAGAGCGTAAAACTTGGTCCGGAGTTGCAAAAGTACCTGGAGGAGATTGCGAACCATCAACGCTATGTAAAGCTGTGTGAAGAGATTGTTTTGGTAAA
CGAGCGTATCTGCGACCTTCGACCAGTGCCCGAAGTGAAAGATGATCAGGAGTTGGCCGAACTTAAAAAAAAATTGCAGAGTCTGTTCATGAAGAAGTAC
AAAATGAGATAGGTCGCATTGTTGGCCGGTATGTTTCAGAAAAAACGACCAACGGCAATTTTGACTTTGAAGCGTTTGAAATGAGCATCCGTTCGTCAAT
GCACCGCATTGGCGGCAGCATACTTGGAATACTCGTAAACGCAGATGGCGGAGGTTACCGGGGTAAAACCATTACCTGCGGGCAAGGGCATCAGTATGAG
TTTATCGATTACCGGAACAAGGAACTGCTCACTGTGTTGGGCCCGGTGGCCGTTAAGCGGGCATACTATTATGATCGAAAATGTCATGTTGGTTTTTGCC
CGAAAGACCGTGATCTGGACATAGGGACATCTTACAGTTGTGGGGTACGCCGGATGATGAGCAAGGTTGGCGCCTACCGGCCATTCGGCCTGGGTCATCA
AGACTTGTATGAGCTGGCGGATATCCGTGTCAACACAAAAGCAGTAGAGCGTGTATCACAGACAGTAGGTGCTCAGGTGGAAGCGTTTCATGCAGCCGAG
GCCAGGACGTCGTTGTCGGATAAAATTGTACCGATCAAACCTGTTCCACGGATGTATGTGTGCATGGATGGCACCGGAGTTCCGGTAGTTAAGAAAGAGA
CTGCCGGCAGGCAAGGCAAATGTGAAGACGGTCAGGCAAAGACCAGGGAAGTCAAGCTGGGTTGCATTTTTACCCAGACAGAAGTTGACCGGAAAGGCCG
TCCGGTGCGAGACGATGGGACCACCAGCTACACCGGTGCAATCGAATCGGCTGAGGTGTTTGGACAGCGGATATATCAAGAGGCACTTCGGCGCGGCATG
CGCCGGGCAAAGGAGGTTTGTGTGCTTGGGGACGGGGCCATCTGGATATGGAATATAGCGGATGAACAATTTTATGGTGCTATCCAGATTGTCGATCTGT
TCCATGCGCGTGAACATTACTGGAACGTCGGTAAGGCATATTTCAGTCAGAATAAGGACAAACTGCATCTGTGGACGGAAGAGCGTCGGAAAGAATTGGA
TGATGGAAGGGTCGAAGATGTAATGAATGCCATCAAGGAATGCTCATCTCAATCGGGATGCGACAAATCAATATATGAACGAGAGATCGGTTATTTTGAA
AAAAACAAGGAAAGGATGCGGTACGCCCATTTTAGGAAGCGGGGTCTTTTTATAGGTTCCGGCGTTCTGGAGGCAGGCTGTCGAACCGTGGTTGGACAGC
GGCTGAAACAGTCGGGTATGCACTGGACGGTTAGTGGCGCAAATAGTATAATAGCTCTGCGCTGTAGTATTCTAAGCAACCGTTGGGAAGATTTTTGGGA
ACATCGGGCCGCAATCTGAAATCGTTACCCATAAATTTGTCGCACACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 414 bp | 137 aa | 99 | 512 | + | No |
Description : First part of the transposase
ORF sequence :
MEETIESLEIEREHLYRQLQETGDFRRGIISVVYRKCGKKNCACAKEGHPGHGPQHLWNTTIKGKSYAKSVKLGPELQKYLEEIANHQRYVKLCEEIVLV
NERICDLRPVPEVKDDQELAELKKKLQSLFMKKYKMR
NERICDLRPVPEVKDDQELAELKKKLQSLFMKKYKMR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1353 bp | 449 aa | 467 | 1819 | + | No |
Description : Second part of the transposase
ORF sequence :
KKIAESVHEEVQNEIGRIVGRYVSEKTTNGNFDFEAFEMSIRSSMHRIGGSILGILVNADGGGYRGKTITCGQGHQYEFIDYRNKELLTLGPVAVKRAYY
YDRKCHVGFCPKDRDLDIGTSYSCGVRRMMSKVGAYRPFGLGHQDLYELADIRVNTKAVERVSQTVGAQVEAFHAAEARTSLSDKIVPIKPVPRMYVCMD
GTGVPVVKKETAGRQGKCEDGQAKTREVKLGCIFTQTEVDRKGRPVRDDGTTSYTGAIESAEVFGQRIYQEALRRGMRRAKEVCVLGDGAIWIWNIADEQ
FYGAIQIVDLFHAREHYWNVGKAYFSQNKDKLHLWTEERRKELDDGRVEDVMNAIKECSSQSGCDKSIYEREIGYFEKNKERMRYAHFRKRGLFIGSGVL
EAGCRTVVGQRLKQSGMHWTVSGANSIIALRCSILSNRWEDFWEHRAAI
YDRKCHVGFCPKDRDLDIGTSYSCGVRRMMSKVGAYRPFGLGHQDLYELADIRVNTKAVERVSQTVGAQVEAFHAAEARTSLSDKIVPIKPVPRMYVCMD
GTGVPVVKKETAGRQGKCEDGQAKTREVKLGCIFTQTEVDRKGRPVRDDGTTSYTGAIESAEVFGQRIYQEALRRGMRRAKEVCVLGDGAIWIWNIADEQ
FYGAIQIVDLFHAREHYWNVGKAYFSQNKDKLHLWTEERRKELDDGRVEDVMNAIKECSSQSGCDKSIYEREIGYFEKNKERMRYAHFRKRGLFIGSGVL
EAGCRTVVGQRLKQSGMHWTVSGANSIIALRCSILSNRWEDFWEHRAAI
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1721 bp | 572 aa | 99 | 1819 | + | Yes |
Chemistry : DDE
ORF sequence :
MEETIESLEIEREHLYRQLQETGDFRRGIISVVYRKCGKKNCACAKEGHPGHGPQHLWNTTIKGKSYAKSVKLGPELQKYLEEIANHQRYVKLCEEIVLV
NERICDLRPVPEVKDDQELAELKKKIAESVHEEVQNEIGRIVGRYVSEKTTNGNFDFEAFEMSIRSSMHRIGGSILGILVNADGGGYRGKTITCGQGHQY
EFIDYRNKELLTLGPVAVKRAYYYDRKCHVGFCPKDRDLDIGTSYSCGVRRMMSKVGAYRPFGLGHQDLYELADIRVNTKAVERVSQTVGAQVEAFHAAE
ARTSLSDKIVPIKPVPRMYVCMDGTGVPVVKKETAGRQGKCEDGQAKTREVKLGCIFTQTEVDRKGRPVRDDGTTSYTGAIESAEVFGQRIYQEALRRGM
RRAKEVCVLGDGAIWIWNIADEQFYGAIQIVDLFHAREHYWNVGKAYFSQNKDKLHLWTEERRKELDDGRVEDVMNAIKECSSQSGCDKSIYEREIGYFE
KNKERMRYAHFRKRGLFIGSGVLEAGCRTVVGQRLKQSGMHWTVSGANSIIALRCSILSNRWEDFWEHRAAI
NERICDLRPVPEVKDDQELAELKKKIAESVHEEVQNEIGRIVGRYVSEKTTNGNFDFEAFEMSIRSSMHRIGGSILGILVNADGGGYRGKTITCGQGHQY
EFIDYRNKELLTLGPVAVKRAYYYDRKCHVGFCPKDRDLDIGTSYSCGVRRMMSKVGAYRPFGLGHQDLYELADIRVNTKAVERVSQTVGAQVEAFHAAE
ARTSLSDKIVPIKPVPRMYVCMDGTGVPVVKKETAGRQGKCEDGQAKTREVKLGCIFTQTEVDRKGRPVRDDGTTSYTGAIESAEVFGQRIYQEALRRGM
RRAKEVCVLGDGAIWIWNIADEQFYGAIQIVDLFHAREHYWNVGKAYFSQNKDKLHLWTEERRKELDDGRVEDVMNAIKECSSQSGCDKSIYEREIGYFE
KNKERMRYAHFRKRGLFIGSGVLEAGCRTVVGQRLKQSGMHWTVSGANSIIALRCSILSNRWEDFWEHRAAI
Blast result :
Comments
ISDesp4 is 42% aa similar to ISDpr10.
The third ORF is the putative ORFAB transposase reconstructed in silico buy possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico buy possible -1 frameshift.
References