ISCysp24
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011884 | ND | Cyanothece sp. | Cyanothece sp. PCC 7425 |
DNA section
IS Length : 1170 bp
Ends
IR Length : 25/29
IRL : GGGAGCGTGTCACCTTTTGGGTAGAAAAGACAAGATGGAGAAGAGCCCTT
IRR : GGGAGCGTGTCACTTTTTTGAGAGAGAAGTGGGGTCCAGTTGCCCATTGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAGTTTATCA | ATCAACCAA | TATATCAAAA | 9 |
| TTACTTGTCC | TTCCCACTC | TCCCTCAAGG | 9 |
| CAGTATAGCG | GTAGGGTT | AGAGACGCAA | 8 |
DNA sequence
GGGAGCGTGTCACCTTTTGGGTAGAAAAGACAAGATGGAGAAGAGCCCTTTATCCCCAACTCCTATGACTCCCGAAGCGCAACAGCAATTGAATCAACAT
CTCCAGCAAATCGCCCAGATTTTATATCAGGATGCCCAGGCTCAGGGATTGCCAATGGGGCGGTTAGAAGACATCGAGGTCACCGTCCGAGAGCAGTTGC
AAGCCCATGTGTCGCCACAAATCGGAATTTTTTTATCAACCAGGCAAGTCCCTCCCAGTCAGAGGGATACCAACGAAGGCTGAAAAGTATTCTGGGGGAA
CTGCACCTGAGCCGCGAGCAAGCCTTGCGTCTATCAGTCCAGCCGGGCAGCCAGATCAGCCCTTATCTGGAGAAGTGTTGTCTGAGAGTCAGTGCCCATC
ACTCTTATCGCCAAGCCAGTGAGGAGATTGCGATCCTGACGGGGATCACCGTCAGTGCGAAGACGCAACAACGCCTGGTTCACCGCCAAGACTTTGCCCC
GCCAGGCGTGGACCGTTCTCCTGGTGTAAGTCAACTGAGTTTGGATGGGGGTAACATTCGTCTGCTCACCCCCAAGGGAGAACCCAGTCAGTGGCGGCAG
TACAAAACCTTGCGCCTCAACGGCGATGGGGTAGGAGTGGCGTTCTACCAGTCCAATGAGCAATTGTGTGAGTGGGCAGCAACCTTACCTGTTGCAGACG
TTCTCTATTGCCTGGGGGATGGGCATCCGGGGATTTGGACCCTGTATGCTCAACTGTCACTCGGTCGCGAGCACGAGGAGATTTTGGATTGGTTTCATCT
CAAGGAAAACCTCTACAAGGTGGGCGGTTCACTCAAACGCTTGCAGGAGGCTGAATCTCTCCTGTGGCAGGGGCAAGTGGACGAAACCCTGAAGCTCTTC
GAGCCGTTAAAAAAATCCCAGGCCCAGAGGTTCTGCGACTATTTACGCACCCATCGCAACCGCATTCCCCACTATGCCTATTACCAAGCCGAAGGCATCC
CGATTGGGTCCGGCAGTGTGGAGTCTTGGGTCAAACAGATTGACCGTCGGACTCAGATTTCCGGGGCTCAATGGCGAGAGGATCATGTCCCACAAGTTTT
GGCGCATCGTTGTGCTTATCTCAATGGGCAACTGGACCCCACTTCTCTCTCAAAAAAGTGACACGCTCCC
CTCCAGCAAATCGCCCAGATTTTATATCAGGATGCCCAGGCTCAGGGATTGCCAATGGGGCGGTTAGAAGACATCGAGGTCACCGTCCGAGAGCAGTTGC
AAGCCCATGTGTCGCCACAAATCGGAATTTTTTTATCAACCAGGCAAGTCCCTCCCAGTCAGAGGGATACCAACGAAGGCTGAAAAGTATTCTGGGGGAA
CTGCACCTGAGCCGCGAGCAAGCCTTGCGTCTATCAGTCCAGCCGGGCAGCCAGATCAGCCCTTATCTGGAGAAGTGTTGTCTGAGAGTCAGTGCCCATC
ACTCTTATCGCCAAGCCAGTGAGGAGATTGCGATCCTGACGGGGATCACCGTCAGTGCGAAGACGCAACAACGCCTGGTTCACCGCCAAGACTTTGCCCC
GCCAGGCGTGGACCGTTCTCCTGGTGTAAGTCAACTGAGTTTGGATGGGGGTAACATTCGTCTGCTCACCCCCAAGGGAGAACCCAGTCAGTGGCGGCAG
TACAAAACCTTGCGCCTCAACGGCGATGGGGTAGGAGTGGCGTTCTACCAGTCCAATGAGCAATTGTGTGAGTGGGCAGCAACCTTACCTGTTGCAGACG
TTCTCTATTGCCTGGGGGATGGGCATCCGGGGATTTGGACCCTGTATGCTCAACTGTCACTCGGTCGCGAGCACGAGGAGATTTTGGATTGGTTTCATCT
CAAGGAAAACCTCTACAAGGTGGGCGGTTCACTCAAACGCTTGCAGGAGGCTGAATCTCTCCTGTGGCAGGGGCAAGTGGACGAAACCCTGAAGCTCTTC
GAGCCGTTAAAAAAATCCCAGGCCCAGAGGTTCTGCGACTATTTACGCACCCATCGCAACCGCATTCCCCACTATGCCTATTACCAAGCCGAAGGCATCC
CGATTGGGTCCGGCAGTGTGGAGTCTTGGGTCAAACAGATTGACCGTCGGACTCAGATTTCCGGGGCTCAATGGCGAGAGGATCATGTCCCACAAGTTTT
GGCGCATCGTTGTGCTTATCTCAATGGGCAACTGGACCCCACTTCTCTCTCAAAAAAGTGACACGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 219 bp | 72 aa | 65 | 283 | + | No |
Description : First part of the transposase
ORF sequence :
MTPEAQQQLNQHLQQIAQILYQDAQAQGLPMGRLEDIEVTVREQLQAHVSPQIGIFLSTRQVPPSQRDTNEG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 211 | 1161 | + | No |
Description : Second part of the transposase
ORF sequence :
VATNRNFFINQASPSQSEGYQRRLKSILGELHLSREQALRLSVQPGSQISPYLEKCCLRVSAHHSYRQASEEIAILTGITVSAKTQQRLVHRQDFAPPGV
DRSPGVSQLSLDGGNIRLLTPKGEPSQWRQYKTLRLNGDGVGVAFYQSNEQLCEWAATLPVADVLYCLGDGHPGIWTLYAQLSLGREHEEILDWFHLKEN
LYKVGGSLKRLQEAESLLWQGQVDETLKLFEPLKKSQAQRFCDYLRTHRNRIPHYAYYQAEGIPIGSGSVESWVKQIDRRTQISGAQWREDHVPQVLAHR
CAYLNGQLDPTSLSKK
DRSPGVSQLSLDGGNIRLLTPKGEPSQWRQYKTLRLNGDGVGVAFYQSNEQLCEWAATLPVADVLYCLGDGHPGIWTLYAQLSLGREHEEILDWFHLKEN
LYKVGGSLKRLQEAESLLWQGQVDETLKLFEPLKKSQAQRFCDYLRTHRNRIPHYAYYQAEGIPIGSGSVESWVKQIDRRTQISGAQWREDHVPQVLAHR
CAYLNGQLDPTSLSKK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1097 bp | 365 aa | 65 | 1161 | + | Yes |
Chemistry : DDE
ORF sequence :
MTPEAQQQLNQHLQQIAQILYQDAQAQGLPMGRLEDIEVTVREQLQAHVSPQIGIFFINQASPSQSEGYQRRLKSILGELHLSREQALRLSVQPGSQISP
YLEKCCLRVSAHHSYRQASEEIAILTGITVSAKTQQRLVHRQDFAPPGVDRSPGVSQLSLDGGNIRLLTPKGEPSQWRQYKTLRLNGDGVGVAFYQSNEQ
LCEWAATLPVADVLYCLGDGHPGIWTLYAQLSLGREHEEILDWFHLKENLYKVGGSLKRLQEAESLLWQGQVDETLKLFEPLKKSQAQRFCDYLRTHRNR
IPHYAYYQAEGIPIGSGSVESWVKQIDRRTQISGAQWREDHVPQVLAHRCAYLNGQLDPTSLSKK
YLEKCCLRVSAHHSYRQASEEIAILTGITVSAKTQQRLVHRQDFAPPGVDRSPGVSQLSLDGGNIRLLTPKGEPSQWRQYKTLRLNGDGVGVAFYQSNEQ
LCEWAATLPVADVLYCLGDGHPGIWTLYAQLSLGREHEEILDWFHLKENLYKVGGSLKRLQEAESLLWQGQVDETLKLFEPLKKSQAQRFCDYLRTHRNR
IPHYAYYQAEGIPIGSGSVESWVKQIDRRTQISGAQWREDHVPQVLAHRCAYLNGQLDPTSLSKK
Blast result :
Comments
ISCysp24 is 68% aa similar to ISMae42.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References