ISMae43

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010296 ND Microcystis aeruginosa
Microcystis aeruginosa NIES-843
DNA section
IS Length : 1262 bp

Ends


IR Length : 32/37

IRL : GGGAGCATCTCACTTATGCAATGAGTATTAATACTTATAGCCGTCAACAA
IRR : GGGAGCATCTCACCTTTGCAATAAGTAGAAATGCTTAATGATCTCAATAA

Insertion site


Left flankDirect repeatRight flankDR Length
CCCGTTTCCCGTTTCCCGCCAGGAATAA8
AGCTATGTACTCTTTAGCGGAATGCCAC8
CATTTAAAACTTAAAACA/TTTAAAACAGT8
GAATAGCTTGTAAACTGAAT0
TAATTTAACCATGCGTTATG0

DNA sequence

GGGAGCATCTCACTTATGCAATGAGTATTAATACTTATAGCCGTCAACAACTAGAAAATCTTCAACTTGATCACAAAGAGAAATGAGATTATAATAGTTA
TAACTTACAATTCAGAATAGCTCTCGATGGGAGGAATAATTCAATGTTATCAGAAAATGAAAAAAGAATTAAAGAGTTATGTCAAGAGTTAGGGCAATGT
CTCTATGAGCAATCCCCAATTAATAAATTTAATAACTTGGGAGAGATAGAGGAGACAGTCAGAGATTTAATGATTCAGTATGTCAACCCAGAAATCGGTA
TTTTTTTGTCAAAACAAGCACAGGAGAAACAGCTGGTCGGACAAGAAAAGTGAAAAGTATTTTGGGGGAATTACCAATTACAGAAAAACAAGCGAAAAAA
TTAGAAATAAAGTCTCGGACTCAGATGAGTCCAATGTTAGAGAAGAACTGTTTGCTATTAAGTGGCGATGAATCCTACGAGAAATCGGCGCAGAAAATCA
AATCATTGACAGGGATTGCTGTTTCTCACAGTACCCAACAACGCCTCGTACATCGCTATGCTTTTGAAGAATTACCGTCTAACCCAGAAGTGGAAGTGGA
AGAAATGAGCCTAGATGGCGGTAAGGTACGACTAAGAACTGCCAAGGGAAAAGCCTTAATTTGGCGTGATTATAAAGCAGTGAGTTTTCATCAACTGGGG
GTAGCGGCTTTTTTTCAAGATAACTCAGCTTTATTAGATTTGGTTAATTCTCAAGTTTTGGCCAAGCCTTTAATTTGTTTAGGAGATGGACATGATGGTA
TCTGGAATTTATTTCGTGAGATAGGAGAGAAACAGGAAAGAATTGAAATATTGGATTGGTATCATTTAATCGAAAACCTCTATAAAGTTGGCGGGTCATT
CCAGCGAATTGAGGAGGTAAAATGTTTTCTCTGGAAGGGGGAAGTGGAGGCGGCTATCTCTTGTTGTGAAGGATGGTCAGAGCCGCAAGTTGAGAATTTT
ATTACTTATTTAAACAAGCATAAACATCGAATTGTCAATTATGGTTATTTGCAGGCAGAGGGGATTTCCATTGGCTCTGGCTCCGTCGAATCAAAAATTA
AACAAATTGCTCATCGTCTTAAAATTACTGGTGCAAGTTGGGAATCTGGTAATGTACCGCAAGTCCTTCGTCATCGCTGTGCTTATCTAAATGGTTGCCT
TTTTTAACTTTTTTATTGAGATCATTAAGCATTTCTACTTATTGCAAAGGTGAGATGCTCCC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
310 bp69 aa144453+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MLSENEKRIKELCQELGQCLYEQSPINKFNNLGEIEETVRDLMIQYVNPEIGIFLSKQAQEKQLVGQEK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
972 bp323 aa2361207+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LGRDRGDSQRFNDSVCQPRNRYFFVKTSTGETAGRTRKVKSILGELPITEKQAKKLEIKSRTQMSPMLEKNCLLLSGDESYEKSAQKIKSLTGIAVSHST
QQRLVHRYAFEELPSNPEVEVEEMSLDGGKVRLRTAKGKALIWRDYKAVSFHQLGVAAFFQDNSALLDLVNSQVLAKPLICLGDGHDGIWNLFREIGEKQ
ERIEILDWYHLIENLYKVGGSFQRIEEVKCFLWKGEVEAAISCCEGWSEPQVENFITYLNKHKHRIVNYGYLQAEGISIGSGSVESKIKQIAHRLKITGA
SWESGNVPQVLRHRCAYLNGCLF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1064 bp354 aa1441207+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLSENEKRIKELCQELGQCLYEQSPINKFNNLGEIEETVRDLMIQYVNPEIGIFFVKTSTGETAGRTRKVKSILGELPITEKQAKKLEIKSRTQMSPMLE
KNCLLLSGDESYEKSAQKIKSLTGIAVSHSTQQRLVHRYAFEELPSNPEVEVEEMSLDGGKVRLRTAKGKALIWRDYKAVSFHQLGVAAFFQDNSALLDL
VNSQVLAKPLICLGDGHDGIWNLFREIGEKQERIEILDWYHLIENLYKVGGSFQRIEEVKCFLWKGEVEAAISCCEGWSEPQVENFITYLNKHKHRIVNY
GYLQAEGISIGSGSVESKIKQIAHRLKITGASWESGNVPQVLRHRCAYLNGCLF

 

Blast result :
Comments
ISMae43 is 66% aa similar to ISMae42.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References