ISSiac4

  • Family ISKra4
  • Group ISKra4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_019892 ND Singulisphaera acidiphila
Singulisphaera acidiphila DSM 18658
DNA section
IS Length : 1519 bp

Ends


IR Length : 17/23

IRL : CGGTGAGCGTCTTGACAGTTGGTGGAATCTTTGTACACAAGAGGGAAACC
IRR : CGGTGAGCATCACATTAGTTGGTAGGACGGACGTTCAGTTGCTGGAATAG

Insertion site


Left flankDirect repeatRight flankDR Length
TCACAACCTGTGCTGGTCTTTGGTTCGATTG9
CTGTGAATGCGCCATAATTCAGGTGAGTCTC9
GAGTAAACCAATGTTGGCGGCGGGAATGATG9
CCGCCAGACGGCCCTTGTTCGACCGTTCGAT9
CGCTCATTCAAGGCCGCGAGGGACGGGTTGT9
TCCTCGCGGCCCCGGGAATACAGCCTTCCGG9
CAGGATGGACAGTCCTACGACTTCAGCGGAT9

DNA sequence

CGGTGAGCGTCTTGACAGTTGGTGGAATCTTTGTACACAAGAGGGAAACCGGCGGTACCTGAGGAAAACCCGACCTCGGAAACGGAGTCCATCCATGCCG
ACCGCACCCGTGCCTCCCGAGAAACAGGCTGAAATCGCGGGGCTCGAGCAAGCCATCCGCGAGGCCGGCGATGCCGAAATCAGCGAACTCGCGGCCAATC
TTGCCATCACCGACGACGCCCACCTCTTCGGCGCCAACGAATTCAAGATCCGGGCCCTGGCTCACAAGATCGCCGCCAAGGCGATCGAGCAGCACCTGAC
GCGAAAAAAAACGGATACGACGGAGCCAGCGTAACCTGTCCCCACTGTAGCCGGACGGCGGAGTTTCATTCGCACCGCCTGCACACCTCCTTCACCCTTG
TGGGAGCCGTCCGCTACCGCCGGGCTTACTACCTCTGTCGATGCTGTGGCAAGGGGCTGTTCCCCTTCGATCGCGAAGCGGGGCTGACGACCCGCGACTT
GACGCCGGCTTTGGAACGCGTCGCGAGCTTGGCCGGGGCGGTTGCCGACAGCTTCGAGAAGGGGGCGGAACTGCTCGAAGAGATGGGGGGTGTCCGGGTC
TCGGAATCGACTGTCGAACGGACCACCGAAGAGGCCGGTCAACGTCTGGCTGAGGCGGTCCAGGCCGGCTCGCACTTGGGTCCCCCGGCCGACTGGCCCT
GGCACAAGGATTACGAAGGCCAACGTTGTGCGTACGTCGAACTCGACGCCACTGGGGTGCGGCGTCAAGGGGAGGGAGGGGGACCCGCCGAAGGCCGCAT
GGCCTACGTGGGGATGGTCTGTAACCCCACCCCGGAGTGGCCTTGGCCCGACGAGAAACCGCAGCCGATGCAGGCGCGTTACCTCGCGGGCCTGTATCCC
TTGGAGGAGTTCGGTCCCTTGCTCCGCACGCCCGCCGGAGACGTCGGTATGGACCGGGCCGATCGCTGGATCGGCCTCTCCGACGGCGGTGCGGGCCTGG
AGGATCGACTCCGTGAAAACTTTCCGCGTGTGGAAGTGATCATTCTTGACTTTTTTCACCCTGCGGAGAAGCTCACCGGCTTGGCGCGTCTGCTGCACCC
TCAGGACGAGGACCAAGCCGAAGACCAAGCCCGGCGATGGCGACAGTTGCTCAAAGAGGAAGGGGGCGCGGTGTTGGGATCTGTCCTGCGGGAATGGGAC
TGGCCGCGCCGGCCTGGCCTGGGCGAAGCCGCGACCGAGTTAATCGGTTACCTTGAACGCCAGGCGCACCGGATGGACTATCCCGAATACCTGGCGCGTG
GCTGGTGTATCGGGAGCGGGGCGATCGAGAGCGCGTGCAAGACGGTGGTGGGGCAGCGTCTGAAACTGGCCGGAATGCGTTGGGGCGAAGACGGCGCTCA
CGCCCTCTGCCACCTCCGCGCCCTCTACCGCAGCGAAAAAGGCCAGTGGGACGCCTTCTGGAATCGCGACTATTCCAGCAACTGAACGTCCGTCCTACCA
ACTAATGTGATGCTCACCG
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
240 bp79 aa95334+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPTAPVPPEKQAEIAGLEQAIREAVDAEISELAANLAITDDAHLFGANEFKIRALAHKIAAKAIEQHLTRKKTDTTEPA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1353 bp450 aa1331485+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

NRGARASHPRGRRCRNQRTRGQSCHHRRRPPLRRQRIQDPGPGSQDRRQGDRAAPDAKKNGYDGASVTCPHCSRTAEFHSHRLHTSFTLVGAVRYRRAYY
LCRCCGKGLFPFDREAGLTTRDLTPALERVASLAGAVADSFEKGAELLEEMGGVRVSESTVERTTEEAGQRLAEAVQAGSHLGPPADWPWHKDYEGQRCA
YVELDATGVRRQGEGGGPAEGRMAYVGMVCNPTPEWPWPDEKPQPMQARYLAGLYPLEEFGPLLRTPAGDVGMDRADRWIGLSDGGAGLEDRLRENFPRV
EVIILDFFHPAEKLTGLARLLHPQDEDQAEDQARRWRQLLKEEGGAVLGSVLREWDWPRRPGLGEAATELIGYLERQAHRMDYPEYLARGWCIGSGAIES
ACKTVVGQRLKLAGMRWGEDGAHALCHLRALYRSEKGQWDAFWNRDYSSN

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1391 bp463 aa951485+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTAPVPPEKQAEIAGLEQAIREAVDAEISELAANLAITDDAHLFGANEFKIRALAHKIAAKAIEQHLTRKKNGYDGASVTCPHCSRTAEFHSHRLHTSF
TLVGAVRYRRAYYLCRCCGKGLFPFDREAGLTTRDLTPALERVASLAGAVADSFEKGAELLEEMGGVRVSESTVERTTEEAGQRLAEAVQAGSHLGPPAD
WPWHKDYEGQRCAYVELDATGVRRQGEGGGPAEGRMAYVGMVCNPTPEWPWPDEKPQPMQARYLAGLYPLEEFGPLLRTPAGDVGMDRADRWIGLSDGGA
GLEDRLRENFPRVEVIILDFFHPAEKLTGLARLLHPQDEDQAEDQARRWRQLLKEEGGAVLGSVLREWDWPRRPGLGEAATELIGYLERQAHRMDYPEYL
ARGWCIGSGAIESACKTVVGQRLKLAGMRWGEDGAHALCHLRALYRSEKGQWDAFWNRDYSSN

 

Blast result :
Comments
ISSiac4 is 43% aa similar to ISLfe1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References