ISNha10
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007964 | ND | Nitrobacter hamburgensis | Nitrobacter hamburgensis X14 Uncultured bacterium Bio7 BpiB04 Nitrobacter hamburgensis X14 plasmid 1 |
DNA section
IS Length : 1428 bp
Ends
IR Length : 20/24
IRL : GGGGATGTCCCGGGTTCTGTTGAATTTCTGAAGAGGTCGGCGTTGCCCAC
IRR : GGGGATGTCCCGATCGCTGTTGAACTACGTCGGGCCGACGTTGGCGGAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGACCCACG | CACTTCAT | CGCAAGTCGC | 8 |
| TAGGCCGTTC | CATGAATG | GACGCCAGCG | 8 |
| GGGCACTCGT | ATGTCATT | GTTTGTGCGC | 8 |
| GGCTATCGGC | TTTGAGTG | CCCTAAGATA | 8 |
DNA sequence
GGGGATGTCCCGGGTTCTGTTGAATTTCTGAAGAGGTCGGCGTTGCCCACCTTGAGCCGGTAGGCTCGGGGTGCTGATTCCACAAGGAGAGCAACGCCAT
GACGAGAGATATCACACCGGCTGGTTTGCCGGCGACCGGAGCTGTGGACGAAGCGTTTGCGGAAGTGCGGGCGAGCTTCGATCGGTTCTGCCTTACGGCA
GGGATCGAAGTGCTCGGCACGATGATGGAGGCGGATGTCACGGCGGCCTGCGGGCCGCGCCACGGTCGCGATGCGGCGCGGCGGGCGCACCGTTGGGGCC
GAACGCGGGGACGGATCGGCTTCCACGGCGGCAAGATCGAGGTCGAGCGCCCGCGGGCCCGGGGCGTGGACGGCCGCGAGATCACGATCCCGAGCTGGGA
AACGGCGGCGCAGGAGGACTGGCTCGGTCGCTGGGCGATGAACCTGATGCTGATCAATGTGTCGACGCGCCGGTTCGGTCGCGCTGTCCGGCTGCCCGAG
GGTGACGTGCCGGCACCGCCCGGATCGGGGGTTTCGAAGTCGGCGGCCTCGCGGAGGTTCGTAGCGCTGTCGGCGGCGCGGCTGGCCGACTTCATGGCTG
CCGATCTATCCGCGCTCGACCTTCTGGTGGTGCAAATCGACGGGTTGCATCTCGGCGGCGATCTCGTGCTGGTCGCCGCGATCGGGGTTGACGGCGAAGG
CAACAAGCATCCGCTGGCGCTGGTGGAAGGGGCGAGCGAGAACGCCGCAACGGTTCAGGCCCTGCTGGACAACATGGTCGCGCGCGGGCTCGACCCGACG
ATGCCAAGACTGTTTATCGTCGACGGCGCGAAGGCATTGTCGAAGGCGATCCGCCGCACCTTCGGTTCGGCCGCTGCGATCCAGCGCTGCCAGATCCACA
AGGCGCGCAACATCATGGAACGCCTGCCGAAAGAGCATCATGCGGCCACCCGTCGGGTGCTGCGCCAGGCCTGGGAGCTCGATGACGCCGACAAGGCTGA
AAAATTGATCCGCAATCTCGCGCGTCGACTCGACCAGCAATGGCCTAGCGTAGCGGCCAGCATCCTCGAAGGCCTCGACGAAATCCTGACCATTGTCCGG
TTGAAGCTGCCGAAGGAGCTTCGTCGATCGCTCGCCTGTACCAACATCGCCGAGAACATGATGGGCACCATTCGCCGCGTCACCCGCAACGTCAAACGCT
GGCGGGATGCCGGTATGGCCTTGCGATGGGTCGCGGCCGGCATGATCGAGGCCAACAAGGGCTTCCGACGATTGAAGGCGCACAAACAACTATCGGTACT
ACGAGCGGCCCTTCAAGCTCACCACGATCGCATGACGATCAAACCAGTTGCCCACGTCACGAGGGCTGCGTAACATTCATTCCGCCAACGTCGGCCCGAC
GTAGTTCAACAGCGATCGGGACATCCCC
GACGAGAGATATCACACCGGCTGGTTTGCCGGCGACCGGAGCTGTGGACGAAGCGTTTGCGGAAGTGCGGGCGAGCTTCGATCGGTTCTGCCTTACGGCA
GGGATCGAAGTGCTCGGCACGATGATGGAGGCGGATGTCACGGCGGCCTGCGGGCCGCGCCACGGTCGCGATGCGGCGCGGCGGGCGCACCGTTGGGGCC
GAACGCGGGGACGGATCGGCTTCCACGGCGGCAAGATCGAGGTCGAGCGCCCGCGGGCCCGGGGCGTGGACGGCCGCGAGATCACGATCCCGAGCTGGGA
AACGGCGGCGCAGGAGGACTGGCTCGGTCGCTGGGCGATGAACCTGATGCTGATCAATGTGTCGACGCGCCGGTTCGGTCGCGCTGTCCGGCTGCCCGAG
GGTGACGTGCCGGCACCGCCCGGATCGGGGGTTTCGAAGTCGGCGGCCTCGCGGAGGTTCGTAGCGCTGTCGGCGGCGCGGCTGGCCGACTTCATGGCTG
CCGATCTATCCGCGCTCGACCTTCTGGTGGTGCAAATCGACGGGTTGCATCTCGGCGGCGATCTCGTGCTGGTCGCCGCGATCGGGGTTGACGGCGAAGG
CAACAAGCATCCGCTGGCGCTGGTGGAAGGGGCGAGCGAGAACGCCGCAACGGTTCAGGCCCTGCTGGACAACATGGTCGCGCGCGGGCTCGACCCGACG
ATGCCAAGACTGTTTATCGTCGACGGCGCGAAGGCATTGTCGAAGGCGATCCGCCGCACCTTCGGTTCGGCCGCTGCGATCCAGCGCTGCCAGATCCACA
AGGCGCGCAACATCATGGAACGCCTGCCGAAAGAGCATCATGCGGCCACCCGTCGGGTGCTGCGCCAGGCCTGGGAGCTCGATGACGCCGACAAGGCTGA
AAAATTGATCCGCAATCTCGCGCGTCGACTCGACCAGCAATGGCCTAGCGTAGCGGCCAGCATCCTCGAAGGCCTCGACGAAATCCTGACCATTGTCCGG
TTGAAGCTGCCGAAGGAGCTTCGTCGATCGCTCGCCTGTACCAACATCGCCGAGAACATGATGGGCACCATTCGCCGCGTCACCCGCAACGTCAAACGCT
GGCGGGATGCCGGTATGGCCTTGCGATGGGTCGCGGCCGGCATGATCGAGGCCAACAAGGGCTTCCGACGATTGAAGGCGCACAAACAACTATCGGTACT
ACGAGCGGCCCTTCAAGCTCACCACGATCGCATGACGATCAAACCAGTTGCCCACGTCACGAGGGCTGCGTAACATTCATTCCGCCAACGTCGGCCCGAC
GTAGTTCAACAGCGATCGGGACATCCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1275 bp | 424 aa | 99 | 1373 | + | No |
Chemistry : DDE
ORF sequence :
MTRDITPAGLPATGAVDEAFAEVRASFDRFCLTAGIEVLGTMMEADVTAACGPRHGRDAARRAHRWGRTRGRIGFHGGKIEVERPRARGVDGREITIPSW
ETAAQEDWLGRWAMNLMLINVSTRRFGRAVRLPEGDVPAPPGSGVSKSAASRRFVALSAARLADFMAADLSALDLLVVQIDGLHLGGDLVLVAAIGVDGE
GNKHPLALVEGASENAATVQALLDNMVARGLDPTMPRLFIVDGAKALSKAIRRTFGSAAAIQRCQIHKARNIMERLPKEHHAATRRVLRQAWELDDADKA
EKLIRNLARRLDQQWPSVAASILEGLDEILTIVRLKLPKELRRSLACTNIAENMMGTIRRVTRNVKRWRDAGMALRWVAAGMIEANKGFRRLKAHKQLSV
LRAALQAHHDRMTIKPVAHVTRAA
ETAAQEDWLGRWAMNLMLINVSTRRFGRAVRLPEGDVPAPPGSGVSKSAASRRFVALSAARLADFMAADLSALDLLVVQIDGLHLGGDLVLVAAIGVDGE
GNKHPLALVEGASENAATVQALLDNMVARGLDPTMPRLFIVDGAKALSKAIRRTFGSAAAIQRCQIHKARNIMERLPKEHHAATRRVLRQAWELDDADKA
EKLIRNLARRLDQQWPSVAASILEGLDEILTIVRLKLPKELRRSLACTNIAENMMGTIRRVTRNVKRWRDAGMALRWVAAGMIEANKGFRRLKAHKQLSV
LRAALQAHHDRMTIKPVAHVTRAA
Blast result :
Comments
ISNha10 is 80% aa similar to ISApr10.
References
1] ISfinder annotation (2013)
2] Starkenburg,S.R., Larimer,F.W., Stein,L.Y., Klotz,M.G., Chain,P.S., Sayavedra-Soto,L.A., Poret-Peterson,A.T., Gentry,M.E., Arp,D.J., Ward,B. and Bottomley,P.J. (2008) Appl. Environ. Microbiol. 74 (9), 2852-2863.
2] Starkenburg,S.R., Larimer,F.W., Stein,L.Y., Klotz,M.G., Chain,P.S., Sayavedra-Soto,L.A., Poret-Peterson,A.T., Gentry,M.E., Arp,D.J., Ward,B. and Bottomley,P.J. (2008) Appl. Environ. Microbiol. 74 (9), 2852-2863.