ISMtu1
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AGJJ01000022 | ND | Mycobacterium tusciae | Mycobacterium rhodesiae NBB3 Mycobacterium tusciae JS617 |
DNA section
IS Length : 1474 bp
Ends
IR Length : 20/28
IRL : GAGGTTGTCCCGGTCTTCGTGGAATTTGAGGTGGCGGTCCCTCACCTGAT
IRR : GAGCATGTCCCGAGTCGCGTGGAATTTGGGTGACGATCCACGATGAACTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCGGTCGGG | AAGGCCAA | ATTCACCACG | 8 |
| CGTGCGAGAA | CGTACCCG | GATCGAACAG | 8 |
| CAAGGTTGGC | GTGGGTGGCG | 0 | |
| TGTTCGGGCA | AGGTTGGC | TGCAACCGAC | 8 |
| CATCTTCGAC | GTGGGTGG | CGTGGACGTG | 8 |
DNA sequence
GAGGTTGTCCCGGTCTTCGTGGAATTTGAGGTGGCGGTCCCTCACCTGATGCCCGCCAGATGGTAGGTCTTCGGGTGAACGACCAAGTACACCTCGAAGA
AGGAGTAAGGGACCGCCATGAAGACCGTACCTACTGTTCGCCTCGCTGACACGACCGACGCCGCCGGGTTGCCTGAGCTGCCGGAGGAGATCCGGCTGGC
GATGACCAGCATCGCCGGGGCGGCCCGGGAGGGGTTGTTGGCGATGAGCGTGGCCGCCGGGATGGCGGTGATGCAGACGATGTTTGAGGCCGAGATCGCC
GCGGCGTGCGGGCCGAAGGGCAAGCACGACGCTGACCGGATTTCGGTGCGCCACGGCAGTGGGAAGGGGTCGGTGACCCTTGGTGGTCGGCGGGTGCCGG
TGGCCCGGCCGCGGGCACGCACTCTGGATGGGAGCGAGGTGGCGCTGAGCACCTATGCGCACTTCGCCGCCGATGACCTGCTGACCCAGGTGGTGATGGA
GCGGATGCTGGCCGGGGTGGCCACCCGCCGGCACGCCCGCACTGCGGAGCCGGTGGGCGCCCAGGTCGGCGAGGAGGCGAAATCGACTAGCCGCTCGGCG
ATTTCGCGCCGATTCGTGCGCCAGACCGAGACCGCGCTGGGCGAGCTGATGAGCCGCGACTTGAGCGAGCTGGACATCAAGGTGCTCATGTTGGATGGGG
AGCACATGGCCCAGCGGTGCGTGGTGGTCGCGCTGGCGATCACCGCCGACGGAACGAAGGTCCCGGTCGGGCTGTGGGACGGCTCCACGGAGAACAAGAC
CGTGGTCCGCTCGCTGCTGGCCGACCTGGTCGAGCGCGGCCTGGCCATCGACGATGGGCTGCTGGTCGTCTGCGACGGCGCCAAGGCGCTGTCCGCGGCG
GTGCGTGAGGTGTTCGGCGCCAAGGCCCTCATCCAAAGATGTACCTTGCACAAGCGGAGAAATGTCGCTGATCATCTGCCCGACAAGGACAAGGCCTGGG
TGGACACCAAGTTGGTCAAGGCCTTCGCCCACCCTGACCCAGACACCGGGTTGCGCAACGCGAAAAGCCTTGCCGGGCAACTTGGTAAGAACTATCCCAG
TGCGGCCGCCAGCCTGCGCGAGGGGCTGGAGGAAATGTTCACTGTCGCCCGCCTCGGCATCGACGGCCGCCTCGCCAAGACGTTGACCACGTCCAATCCG
GTCGAGTCGATGATCTCCATCGCCCGGACCACCAATCGCAACGTCACCCGCTGGCGCGACGGGCAGATGGTGCTGCGCTGGACCGCGGCCGGCATGCTCA
ACGCCGAACGATCCTTCCGTCGCATCAAGGGCCACAAGCAGATGCCCCAGCTCGTCGACGCCCTGCGCCGACACGCTCACCCCGACACCGGCGCCGACAC
CAAATCTGTCGGTGCCGCCGCCTAGAGTTCATCGTGGATCGTCACCCAAATTCCACGCGACTCGGGACATGCTC
AGGAGTAAGGGACCGCCATGAAGACCGTACCTACTGTTCGCCTCGCTGACACGACCGACGCCGCCGGGTTGCCTGAGCTGCCGGAGGAGATCCGGCTGGC
GATGACCAGCATCGCCGGGGCGGCCCGGGAGGGGTTGTTGGCGATGAGCGTGGCCGCCGGGATGGCGGTGATGCAGACGATGTTTGAGGCCGAGATCGCC
GCGGCGTGCGGGCCGAAGGGCAAGCACGACGCTGACCGGATTTCGGTGCGCCACGGCAGTGGGAAGGGGTCGGTGACCCTTGGTGGTCGGCGGGTGCCGG
TGGCCCGGCCGCGGGCACGCACTCTGGATGGGAGCGAGGTGGCGCTGAGCACCTATGCGCACTTCGCCGCCGATGACCTGCTGACCCAGGTGGTGATGGA
GCGGATGCTGGCCGGGGTGGCCACCCGCCGGCACGCCCGCACTGCGGAGCCGGTGGGCGCCCAGGTCGGCGAGGAGGCGAAATCGACTAGCCGCTCGGCG
ATTTCGCGCCGATTCGTGCGCCAGACCGAGACCGCGCTGGGCGAGCTGATGAGCCGCGACTTGAGCGAGCTGGACATCAAGGTGCTCATGTTGGATGGGG
AGCACATGGCCCAGCGGTGCGTGGTGGTCGCGCTGGCGATCACCGCCGACGGAACGAAGGTCCCGGTCGGGCTGTGGGACGGCTCCACGGAGAACAAGAC
CGTGGTCCGCTCGCTGCTGGCCGACCTGGTCGAGCGCGGCCTGGCCATCGACGATGGGCTGCTGGTCGTCTGCGACGGCGCCAAGGCGCTGTCCGCGGCG
GTGCGTGAGGTGTTCGGCGCCAAGGCCCTCATCCAAAGATGTACCTTGCACAAGCGGAGAAATGTCGCTGATCATCTGCCCGACAAGGACAAGGCCTGGG
TGGACACCAAGTTGGTCAAGGCCTTCGCCCACCCTGACCCAGACACCGGGTTGCGCAACGCGAAAAGCCTTGCCGGGCAACTTGGTAAGAACTATCCCAG
TGCGGCCGCCAGCCTGCGCGAGGGGCTGGAGGAAATGTTCACTGTCGCCCGCCTCGGCATCGACGGCCGCCTCGCCAAGACGTTGACCACGTCCAATCCG
GTCGAGTCGATGATCTCCATCGCCCGGACCACCAATCGCAACGTCACCCGCTGGCGCGACGGGCAGATGGTGCTGCGCTGGACCGCGGCCGGCATGCTCA
ACGCCGAACGATCCTTCCGTCGCATCAAGGGCCACAAGCAGATGCCCCAGCTCGTCGACGCCCTGCGCCGACACGCTCACCCCGACACCGGCGCCGACAC
CAAATCTGTCGGTGCCGCCGCCTAGAGTTCATCGTGGATCGTCACCCAAATTCCACGCGACTCGGGACATGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1308 bp | 435 aa | 118 | 1425 | + | No |
Chemistry : DDE
ORF sequence :
MKTVPTVRLADTTDAAGLPELPEEIRLAMTSIAGAAREGLLAMSVAAGMAVMQTMFEAEIAAACGPKGKHDADRISVRHGSGKGSVTLGGRRVPVARPRA
RTLDGSEVALSTYAHFAADDLLTQVVMERMLAGVATRRHARTAEPVGAQVGEEAKSTSRSAISRRFVRQTETALGELMSRDLSELDIKVLMLDGEHMAQR
CVVVALAITADGTKVPVGLWDGSTENKTVVRSLLADLVERGLAIDDGLLVVCDGAKALSAAVREVFGAKALIQRCTLHKRRNVADHLPDKDKAWVDTKLV
KAFAHPDPDTGLRNAKSLAGQLGKNYPSAAASLREGLEEMFTVARLGIDGRLAKTLTTSNPVESMISIARTTNRNVTRWRDGQMVLRWTAAGMLNAERSF
RRIKGHKQMPQLVDALRRHAHPDTGADTKSVGAAA
RTLDGSEVALSTYAHFAADDLLTQVVMERMLAGVATRRHARTAEPVGAQVGEEAKSTSRSAISRRFVRQTETALGELMSRDLSELDIKVLMLDGEHMAQR
CVVVALAITADGTKVPVGLWDGSTENKTVVRSLLADLVERGLAIDDGLLVVCDGAKALSAAVREVFGAKALIQRCTLHKRRNVADHLPDKDKAWVDTKLV
KAFAHPDPDTGLRNAKSLAGQLGKNYPSAAASLREGLEEMFTVARLGIDGRLAKTLTTSNPVESMISIARTTNRNVTRWRDGQMVLRWTAAGMLNAERSF
RRIKGHKQMPQLVDALRRHAHPDTGADTKSVGAAA
Blast result :
Comments
ISMtu1 is 67% aa similar to IS1295.
References
1] ISfinder annotation (2013)
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Land,M.L., Hauser,L., Mattes,T., Holmes,A., Rutledge,P., Paulsen,I., Coleman,N. and Woyke,T.J. (2011) Direct submission GenBank.
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Land,M.L., Hauser,L., Mattes,T., Holmes,A., Rutledge,P., Paulsen,I., Coleman,N. and Woyke,T.J. (2011) Direct submission GenBank.