ISBame1
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004604 | ND | Bacillus megaterium | Bacillus megaterium QM B1551 plasmid pBM600 Bacillus megaterium QM B1551 plasmid pBM400 |
DNA section
IS Length : 1341 bp
Ends
IR Length : 20/26
IRL : GAGAGCGTAAAATATCTTGTGTAAATGAATAAATACAAAAAGGAAGACCT
IRR : GGGACCGTCAATAATTTTGTGTAAATACGAATTTCCTTTTCTTATAGACG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAGAGATAA | TTTTATTG/A | TTGCTTACTT | 10 |
| CAAATTGTTG | TGAAAATC | TTTTATATTA | 8 |
| TTATAGTAAA | TTTACGGA | GGTGAGAATA | 8 |
DNA sequence
GAGAGCGTAAAATATCTTGTGTAAATGAATAAATACAAAAAGGAAGACCTTTTCTTGTAGAATTAAGTCACCACAACCAATTCAAAGAAAGAAGGTCCTC
CTATGAATCAGTTTACAACAGATCTTGTTCAAGCTCTAGTTCAAAAAGAAAATGTGACGGAAATTTTCCGTTCTCACTTAGAAAATGCGGTTAATACACT
CTTAGCGAGCGAATTGACAGCTTTTTTGGACTACGAAAAATATGATCGTATTGGATTTAATACAGGGAATTCACGAAATGGTTCGTACGAACGGACGCTG
CACACAGAGTTTGGAGAACTTCATCTCGTTATTCCTCGTGATCGGAATGGCGACTTTAAGCAACAGACGGTGGCTCCTTATAAAAGATCCAATGATACGC
TTGAAGCGTTTGTCATTCATATGTTTCAAAAGGGTGTAACGACAGCTGAAATTGCCCATTTAATGGAGCGCATGTATGGTCATCACTACACGCCTCAAAC
CATTTCAAACATGACAAAGGTGATGTCTGAACAGGTAGAAGCCTTCCGTGATCGAACCTTGTGCGCACGCTATGCGTGCGTTTATTTAGACGCTACATAT
ATTGCTTTAAAACGGGATACAGTGTCTAAAGAAGCTGTCTATATTGCCGTTGGCATTCGCGAAGACGGTTCAAAAGAAGTGCTTGGCTATACAATCGCTC
CTAACGAATCTGCCTTTGTCTGGAAAGAACTTTTAGAAGACATCAAATCTCGTGGCGTAGAGGAGATTCTGTTGTTTATTTCCGACGGGTTAAAAGGAAT
CTCAGACAGTGTTTTTTCTATCTATCCAGCTTCTTCTTATCAAACATGCTGCGTCCATCTTTCACGTACGATTGCTCATAAAGTTCGTGTATCCGATCGA
GCTGAAATCTGTGAAGACTTTAAATCTGTGTACCGTGCAGAAAGCCTTGAAATGGGCCAACAGGCCCTGCATTCGTTTATTGAAAAGTGGAAGTCAAAGT
ACCCAAAAGTTACAAAGCCATTGCTCAATAACTCTTATTTGTTCACGTTTTATAGCTTTCCTAAGTCTATTTGGCGCAGTATTTATTCGACCAATCTCAT
TGAATCTTTTAATAAACAAATCAAGAAATATAGTAAGCGTAAGGAACAATTCCCAAACGAAGAGTCACTTGAACGCTTCCTCGTTTCCCAATTTGACCAG
TATAATCAGCGCTTTGCCACAAGATGTCACATTGGTTTTGATCAAGCTCGCGCAGAGCTACAAATGATGTTTAAAACAAACTAGTGAATAACGTCTATAA
GAAAAGGAAATTCGTATTTACACAAAATTATTGACGGTCCC
CTATGAATCAGTTTACAACAGATCTTGTTCAAGCTCTAGTTCAAAAAGAAAATGTGACGGAAATTTTCCGTTCTCACTTAGAAAATGCGGTTAATACACT
CTTAGCGAGCGAATTGACAGCTTTTTTGGACTACGAAAAATATGATCGTATTGGATTTAATACAGGGAATTCACGAAATGGTTCGTACGAACGGACGCTG
CACACAGAGTTTGGAGAACTTCATCTCGTTATTCCTCGTGATCGGAATGGCGACTTTAAGCAACAGACGGTGGCTCCTTATAAAAGATCCAATGATACGC
TTGAAGCGTTTGTCATTCATATGTTTCAAAAGGGTGTAACGACAGCTGAAATTGCCCATTTAATGGAGCGCATGTATGGTCATCACTACACGCCTCAAAC
CATTTCAAACATGACAAAGGTGATGTCTGAACAGGTAGAAGCCTTCCGTGATCGAACCTTGTGCGCACGCTATGCGTGCGTTTATTTAGACGCTACATAT
ATTGCTTTAAAACGGGATACAGTGTCTAAAGAAGCTGTCTATATTGCCGTTGGCATTCGCGAAGACGGTTCAAAAGAAGTGCTTGGCTATACAATCGCTC
CTAACGAATCTGCCTTTGTCTGGAAAGAACTTTTAGAAGACATCAAATCTCGTGGCGTAGAGGAGATTCTGTTGTTTATTTCCGACGGGTTAAAAGGAAT
CTCAGACAGTGTTTTTTCTATCTATCCAGCTTCTTCTTATCAAACATGCTGCGTCCATCTTTCACGTACGATTGCTCATAAAGTTCGTGTATCCGATCGA
GCTGAAATCTGTGAAGACTTTAAATCTGTGTACCGTGCAGAAAGCCTTGAAATGGGCCAACAGGCCCTGCATTCGTTTATTGAAAAGTGGAAGTCAAAGT
ACCCAAAAGTTACAAAGCCATTGCTCAATAACTCTTATTTGTTCACGTTTTATAGCTTTCCTAAGTCTATTTGGCGCAGTATTTATTCGACCAATCTCAT
TGAATCTTTTAATAAACAAATCAAGAAATATAGTAAGCGTAAGGAACAATTCCCAAACGAAGAGTCACTTGAACGCTTCCTCGTTTCCCAATTTGACCAG
TATAATCAGCGCTTTGCCACAAGATGTCACATTGGTTTTGATCAAGCTCGCGCAGAGCTACAAATGATGTTTAAAACAAACTAGTGAATAACGTCTATAA
GAAAAGGAAATTCGTATTTACACAAAATTATTGACGGTCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1182 bp | 393 aa | 103 | 1284 | + | No |
Chemistry : DDE
ORF sequence :
MNQFTTDLVQALVQKENVTEIFRSHLENAVNTLLASELTAFLDYEKYDRIGFNTGNSRNGSYERTLHTEFGELHLVIPRDRNGDFKQQTVAPYKRSNDTL
EAFVIHMFQKGVTTAEIAHLMERMYGHHYTPQTISNMTKVMSEQVEAFRDRTLCARYACVYLDATYIALKRDTVSKEAVYIAVGIREDGSKEVLGYTIAP
NESAFVWKELLEDIKSRGVEEILLFISDGLKGISDSVFSIYPASSYQTCCVHLSRTIAHKVRVSDRAEICEDFKSVYRAESLEMGQQALHSFIEKWKSKY
PKVTKPLLNNSYLFTFYSFPKSIWRSIYSTNLIESFNKQIKKYSKRKEQFPNEESLERFLVSQFDQYNQRFATRCHIGFDQARAELQMMFKTN
EAFVIHMFQKGVTTAEIAHLMERMYGHHYTPQTISNMTKVMSEQVEAFRDRTLCARYACVYLDATYIALKRDTVSKEAVYIAVGIREDGSKEVLGYTIAP
NESAFVWKELLEDIKSRGVEEILLFISDGLKGISDSVFSIYPASSYQTCCVHLSRTIAHKVRVSDRAEICEDFKSVYRAESLEMGQQALHSFIEKWKSKY
PKVTKPLLNNSYLFTFYSFPKSIWRSIYSTNLIESFNKQIKKYSKRKEQFPNEESLERFLVSQFDQYNQRFATRCHIGFDQARAELQMMFKTN
Blast result :
Comments
ISBame1 is 80% aa similar to ISEf1.
References
1] ISfinder annotation (2013)
2] Eppinger,M., Bunk,B., Johns,M.A., Edirisinghe,J.N., Kutumbaka,K.K., Koenig,S.S., Creasy,H.H., Rosovitz,M.J., Riley,D.R., Daugherty,S., Martin,M., Elbourne,L.D., Paulsen,I., Biedendieck,R., Braun,C., Grayburn,S., Dhingra,S., Lukyanchuk,V., Ball,B., Ul-Qamar,R., Seibel,J., Bremer,E., Jahn,D., Ravel,J. and Vary,P.S. (2011) Appl. Environ. Microbiol. 69 (11), 6888-6898.
3] Kunnimalaiyaan,M., Stevenson,D.M., Zhou,Y. and Vary,P.S. (2001) Mol. Microbiol. 39 (4), 1010-1021.
2] Eppinger,M., Bunk,B., Johns,M.A., Edirisinghe,J.N., Kutumbaka,K.K., Koenig,S.S., Creasy,H.H., Rosovitz,M.J., Riley,D.R., Daugherty,S., Martin,M., Elbourne,L.D., Paulsen,I., Biedendieck,R., Braun,C., Grayburn,S., Dhingra,S., Lukyanchuk,V., Ball,B., Ul-Qamar,R., Seibel,J., Bremer,E., Jahn,D., Ravel,J. and Vary,P.S. (2011) Appl. Environ. Microbiol. 69 (11), 6888-6898.
3] Kunnimalaiyaan,M., Stevenson,D.M., Zhou,Y. and Vary,P.S. (2001) Mol. Microbiol. 39 (4), 1010-1021.