ISfinder Mirror Site

ISBibr1

Family IS256
Group IS1249

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_GG729830	ND	Bifidobacterium breve	Bifidobacterium breve DSM 20213

DNA section

IS Length : 1622 bp

Ends

IR Length : 26/27

IRL : GGGCCTATAGGAACTTTTGTGGTGCTTATAACCTGGCTTTGGCCTTGTGG
IRR : GGCCTATAGGAACTTTTGTGGTGCTTTAGGTTTGTCCTATCTCCGTTCTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCGCCGCTAA	AGAACTC	GGCGTAACCA	7

DNA sequence

GGGCCTATAGGAACTTTTGTGGTGCTTATAACCTGGCTTTGGCCTTGTGGGATTAGGCGTGTCGGGGTGTCGTTTTTCCCGGCGGGAAAATAGGGGCGTT
CCGGTGGTGGGCTGGACGCATGAGAACGAAGTCCGCCAAGGCGAAGAAATGCCCGGTGTGCGGCCGGCCGATGAAGAAGAACGGTCGGACCGCGAAGGGG
GTGCAGCGCTGGAAGTGCGCGGCCTGCTCGTTGAGTTCGACGATGCCGCAGGAGAATGCTGGACGGGCCCGGCAGCTCGACGCCTTCCTCGGCTGGCTGC
TCGGCCGTGCCTCCCAGTCCGCGTGCGACGCGAACGGCGATTCGCGGGCCCTGCGCAAGCGCACCGCGTGGTGCTGGAACGTCCGGCCCGTGATCCCACC
GCCGATGGTGAAACATCATACGGTGATGGCGGACGGCACGTACATGAACCACGGCTGGTGCCTGGTCATAGCCATCGACGGCGAGAGCGGCGAGGTGCTG
GGCTTCCAGTGGTGCTCGAACGGGTCGAAGGCCGCGTACATGGCGTTGTTCTCGCGCATCCCCGCGCCCGACGTGCTGATCACGGACGGGCTGCGCGGCG
CGGAAAGCGCCTGCAACGAGGCCTGGCCGGCCGCCCGCATCCAGCGGTGCCTGGTGCACGTGCAGCGCAACACGAGGACGGACCTGACCTCGAAGCCCAG
GCTGGAGGCCGGCAAGGAGCTCAAGAAGCTCTCCGACGGGCTCACCAAGATCCGTGACGCCGAGGCGGCGGCCAGGTGGGGCGAGGCGCTCAACGCATGG
CACATCCGTTGGAGAGAGTTCATAGCCGAACGCACCTTCGCGAAGGACGACCCCGCCAACCCGAAGGCGTCCAAACAGGAGTGGTGGTGGACCCACCAGG
AGCTGCGCCGCTGCTACCGGCGGCTGGAGAGACTGTTCCGCGAGGGCAAGCTGTTCGCGTTCCTCGAACCCAAACTCACGGCCGCCGGCCCGGTGGCGCG
CACCACGAACCGGTTGGAGGGCGGCGTCAACTCGCCGCTCAAGCACGTGCTCCTCGACCACCACGGGCTGCCCGAGGAACATATGAGGCGAGCCTGCGAA
TGGGTGTGCTACATGAAAAGCGGGAATCCCGCCCCCGGCTCATTGATCAGGGCCGGGCACTGGAGACCGGCAAGGCCGTCACCGGTCGAGGAGGAGACCG
GTGCCGGCACCGGCGCACCGAGATATGGCACCGGAATCAGCTGGGACGAGTTCCATGCCCACGTCTCGTGGCGGTCCGACGACTGACCGCCGGTCTCCAT
GCATTTGGGCCGGTACCACGTGCGCTCCGGACGCATAGACGCCGGCATGGCCCAAATCATTGAAACCATATTGCAATTCATCCAGATCTTGTCCGACCTC
GTAGAGGCGCTCGGCAGCGGTTCTGCCGACGGACACTGGCTGCCGCTCGTCTCCTCCGTTGCGGGATTGCTGCTGTTCGTTCTCCGACTCCTTGAGATCG
GCAAACGCCCCGCTCGTCACAGTGCCCGTCACAGGAACAAACGCCACACGAGAACAGGACGCGGCCGCCACTCAGAACGGAGATAGGACAAACCTAAAGC
ACCACAAAAGTTCCTATAGGCC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1167 bp	388 aa	120	1286	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRTKSAKAKKCPVCGRPMKKNGRTAKGVQRWKCAACSLSSTMPQENAGRARQLDAFLGWLLGRASQSACDANGDSRALRKRTAWCWNVRPVIPPPMVKHH
TVMADGTYMNHGWCLVIAIDGESGEVLGFQWCSNGSKAAYMALFSRIPAPDVLITDGLRGAESACNEAWPAARIQRCLVHVQRNTRTDLTSKPRLEAGKE
LKKLSDGLTKIRDAEAAARWGEALNAWHIRWREFIAERTFAKDDPANPKASKQEWWWTHQELRRCYRRLERLFREGKLFAFLEPKLTAAGPVARTTNRLE
GGVNSPLKHVLLDHHGLPEEHMRRACEWVCYMKSGNPAPGSLIRAGHWRPARPSPVEEETGAGTGAPRYGTGISWDEFHAHVSWRSDD

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
288 bp	95 aa	1299	1586	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MHLGRYHVRSGRIDAGMAQIIETILQFIQILSDLVEALGSGSADGHWLPLVSSVAGLLLFVLRLLEIGKRPARHSARHRNKRHTRTGRGRHSERR

Blast result :

Comments

ISBibr1 is 55% aa (ORFA : the transposase) similar to ISAar5.
The second ORF is passenger gene annotated as hypothetical protein.

References

1] ISfinder annotation (2013)
2] Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B.,
Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. (2009) Direct submission GenBank.