ISfinder Mirror Site

ISCoam1

Family IS256
Group IS1249

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_GG771298	ND	Corynebacterium ammoniagenes	Corynebacterium ammoniagenes DSM 20306

DNA section

IS Length : 1379 bp

Ends

IR Length : 21/24

IRL : GGGCTAAGGGAACAGAAGTGTGTCCAGGATCCTGATATATCCCCAGGTTG
IRR : GGGCTAAGGGAACAAAAACGTGTCTATCGGCGTGTCGGGTTAGGTGCCGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

GGGCTAAGGGAACAGAAGTGTGTCCAGGATCCTGATATATCCCCAGGTTGTCTCTAATAAAGCGTAGGTATGTAATCTTTTCAGTGTTATAAACAGCTCA
ATCTCGGTCAATACCGGGATCAGTACCAAACCTGTGTTACCTGCCGTTATCTGCTGGTGGAATGACGAAAAACCATCCACTATGCCCGGTCTGCCATGAG
CTGATGAAACGCAATGGATTTACCTCCAAAGGCACACAACGCTGGCGCTGTAAATCCACAACCTGCGGCGCTAGCACCACGAAACAACGCCGCGATAAGC
GACTCGCTGCGGACTTTAAACGCTTCATCACCCATGTCACAGGCCAACAATCCATGGACACCATCGCCTGTGACATGGGAATATCACGCCGCTACCTCAG
CACCCGGTTTCGCACCTTCTGGTACATCCCCACCCCAGACCCAGTCGATCCCCACCGCATCTTCGACCAACTATTTATCGACGGAACCCACCTCTCTGCC
GGTTGTCTACTAATTGCCTCCACGAAAAACCACATCGTCAACTGGGTTTGGGCGAAAGAAGAAAACACCCATGACTACCTCAGGCTGCTCCAACCGATCC
CCGCACCCCTGATGGTTTGCTTAGACGGTGGACGCGGTGCCTACAGCGCCATCAAAAAAGACTGGCCCAGCACACGCATCCAACGCTGCCTCGTCCACGC
CCAACGCGTCGTACGCCGCTATGTCACCTCACAACCACGCACCAACGCCGGACAAGACATCTACCGCCTGGCACGCACACTCAGCCGAATTACCGACCTG
GACCAAGCAGCTCAATGGTCGGCACAACTGCACAACTTCTATGTGACATACAAGGACTACCTCAACGAAAAGACCTTGTCGATCATCACCGGGAAAAGAG
AATTTACCCACCTTCGAGTGCGTAAAGCCTATTACAGTCTCGAAAACCTCGACCGTAAACAATGGCTATTTACCTACCTCAACCCACCACAGAATCCGGC
TGACCCCGATATTAAGTGGGCCTGCACCACCAACAGCCTGGAAGGCGGATTTAACTCCCCACTCAAACAACACGCCAGACTCCACCGAGGAAGAAGCGGT
GAACGACAACGCAAAACCATCGACTGGTGGCTATACCTGCGCACACATCGCCCGAAAGACCCACAAGAAATAGCCAAAGAATGCAATTGGGGCCGTGACG
CACTGTCCAAGGTAAACGCCACGACCCACAACGAGAACCAAGCCAACCAAGAAACAGGAAGACCAGCCCTCTACGACAACGCTATCTCAACCGAATACAG
CCACAACCTCGGCATCCAAAAAGGCTGGATCGGCACCTAACCCGACACGCCGATAGACACGTTTTTGTTCCCTTAGCCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1179 bp	392 aa	162	1340	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKNHPLCPVCHELMKRNGFTSKGTQRWRCKSTTCGASTTKQRRDKRLAADFKRFITHVTGQQSMDTIACDMGISRRYLSTRFRTFWYIPTPDPVDPHRI
FDQLFIDGTHLSAGCLLIASTKNHIVNWVWAKEENTHDYLRLLQPIPAPLMVCLDGGRGAYSAIKKDWPSTRIQRCLVHAQRVVRRYVTSQPRTNAGQDI
YRLARTLSRITDLDQAAQWSAQLHNFYVTYKDYLNEKTLSIITGKREFTHLRVRKAYYSLENLDRKQWLFTYLNPPQNPADPDIKWACTTNSLEGGFNSP
LKQHARLHRGRSGERQRKTIDWWLYLRTHRPKDPQEIAKECNWGRDALSKVNATTHNENQANQETGRPALYDNAISTEYSHNLGIQKGWIGT

Blast result :

Comments

ISCoam1 is 70% aa similar to ISCge1.

References

1] ISfinder annotation (2013)
2] Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C.,
Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Zhang,X., Suruliraj,S., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. (2010) Direct submission GenBank.