ISfinder Mirror Site

ISTni2

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_019902	ND	Thioalkalivibrio nitratireducens	Thioalkalivibrio nitratireducens DSM 14787

DNA section

IS Length : 3451 bp

Ends

IR Length : 13/24

IRL : TGAGCACGGTCATGGCTGCTGATGGGATTTGCGGCTGGAATGATCAGGCA
IRR : TGAGTGTGATCACCTGATTTGATGTTGCGCTGATCACGTTCGTTGAGGGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GATGCGCAGC	GCCAGG	GACGCTTGCA	6

DNA sequence

TGAGCACGGTCATGGCTGCTGATGGGATTTGCGGCTGGAATGATCAGGCAGCGTGAGGTTTGAGGGGGGATCCCCCTCGGGGAGCTTGAACAAACGCGCC
CGTCGGGGGAGTCTCTGGTTACCACGCCAAGAGACGCTCACAGTGTGCCACGTTCCGTTGTCCGATCCCCAGTTTTTCCGCTTTCTGACCTACATCGATG
AGACCGTGGCGGCTGAGGTCCAGGCGGCGGGGTGTCCGTACTGTGGCGGTGCCTTGCATCAGGCGAACTATCCGCGCAAGCCGCGTGGCTGTCCGGGTGC
GTTCCGCGTGGAGTATTCGACCCGGCTGAGTTTTTGCTGTCATCAGTGTCGGCGCCGGACCACCTCGAGGTCGGTGCGGTTCCTGGGGCGGCGGGTGTGG
TTGGGCTGGGTGGTGGTGTTGGGGTCGACCCGGGTGGGCGAGCGGACCCCGGCGGCATCCGCACTGTGTGAAGCGTTGTCGATTCCCTGGCGGACTCTGG
CGCGGTGGCGCCGGTGGTGGCGCGAAGGCTTTGCGCAGAGTGCGGTGTGGCAGGTGCTGGGTGCGCGGTTCCTGCCGCCGGTGGCGGTGGTACGGTTGCC
GGCCGGTCTTCTGGAGCGCATCCCGGGCCAGGGGCAGGCACCGTTGCTTGCGTTACTGACGTGGCTCTGGCCGCTCACGGTCACGGCTCCCGAGGGGCGC
TGAGTTTCCGCAGAGGATGTCAATAGGCAGAGCCGAGCGTAACAAATAGCCTCCTCAGGAGGGTGGTACCGCCGGTACCGCCGACACTGGGAGAAGGCTT
TTGACCACAGGCAACGATCCACGCCACCGCGATCGCTGGGCGCGGCTGCGCTTCTCGATCATTGGTCCTTTGTTCGCGGCGCCACCCCAGCCGGGTGCGT
TGCAAGCGGCGCTGGCAGCACTGGCCGAACGCGACTGGCGGCATCCGGTCACCGGAGGCCCTGTGCGCTTCGGCGTGTCGACGCTAGAGCGCTGGTACTA
CGCGGCCCGCGCGGCCACCGATCCGGTGGCCGCCTTGCGCAACCGGCGCCGGCCCAAGGGCCACTTCATGGCCCTGACGCCGGTAGTGATCGAGACCCTG
GTGCAGCAGTACCGGGAGCATCCCGGCTGGACCGCGCAGTTGCACTTCGACAACCTGTGTGCGGCCTTCGCCGGCTGCACGGAGGGGATGCCCTCGTACG
CCACGGTGCGGCGGTACCTGAAGGCGCACGGGATGCACCGTCAGGCGCGTCCGCGCCGGGATACTGAAGGGGCCGAGCGGGCCCGTGACCGGTTCGAACG
GCTCGAGGTACGCAGCTACGAGCTGGAGCATGTCGCGGCCCTGTGGCATCTGGACTTCCATCACGGCTCGCGCAAGGTGCTGACCCGGGACGGAACCTGG
GCCAAGCCGTATCTGCTCGGCATCCTGGACGATCGCTCGCGCCTGATCTGCCATCTCCAGTGGTACCTGGACGAGAGCGCCCAGGGTCTGGTGCACGGCC
TGTCGCAGGCGTTCATGAAGCGCGGCCTGCCGCGGGCCTTGATGACCGACAATGGCGCTGCGATGCTGGCCGAGGAAACCGTCGCCGGACTGCAACGCCT
GGGCGTGCTGCACCAGACCACGCTACCGTATTCTCCGCATCAAAACGGTAAGCAGGAGTCCCTCTGGGGACGTATCGAAGGCCGTCTGCTGCCGATGCTG
GAAGGGCACACGCCACTCACCCTGGAGGTGCTGAACCAGGCCACGCAGGCCTGGGCCGAGCAGGAGTACCACCGCACTCACCACAGTGAACTCGGCACCA
CCCCGCTGCAACGCTATCTGGCCGGACCCAACGTACGCCGGGACTGCCCGCCGGTCGCGGCCCTGGCCGACGCCTTCCGCATCGAGGTCACCCGCCGCCA
GCGGCGCTCCGACGGCACCGTCTCGCTGGACGGGCAGCGCTTCGAAATCCCCGCCCGCTACCAGCACCTCGACCGGGTGCACCTGCGTTACGCGCGCTGG
GATCGCAGTCGCGTCGATCTGGTCGATCCGCACACCGGCACCCTCCTGTGCCCGGTGCTGCCGCTCGACAAGTCCGCCCACGCCCACGGCCAACGCCGCA
CCCGACCCCCTGCCACGCCCCCGCTGGATCCGCTGCCGCCCTCGGGCATGGCGCCATTGTTACGCCAGCTGCTCGCCGACTACGCCGCCACCGGTCTGCC
GCCAGCCTATCTGCCGATCCCCGAGGAGGACGACGCATGAACCCGAAATTACTCGCCCTCTACGGGCTGAAGTGGAACCCGTTCGCCACCGAAATCCCGA
TCGAGGCACTCTATGTGCCAGCACGGGTCGAGGACTTCTACTGGCGCATCGAGCAGGGACTGATCCGGGAAGGTGGCTTCGCCATGGTCCACGGCGACCC
CGGCACCGGCAAAAGCGCCGTCCTGCGCCTGATCGCGGAACGCCTGGCACGGCTGCCCGACCTCACCGTCGCCGCGATCCATCACCCGCAGAGCCATCTC
GCCGACTTCTACCGCGAGATGGGTGATCTCTTCGGCGTGCCCCTGCGCCCGCACAACCGCTGGGGTGGCTTCAAGGCTCTGCGCGAGAACTGGTTCGCCC
ACCTGGACACCACGCGCCGGCGCGCCGTGCTCCTGATCGACGAAGCCCAGGAAATGAGCCCCGCGGTCCTGTCCGAACTGCGCCTGATGGCCAGCGCCCG
GTTCGACTCCCAGACCCTCCTGTGCGTGGTCCTCGCCGGCGACGCCCGCCTGCCCGAAAAGCTGCGCCGCGAGGAACTCATCCCGCTCGGCTCGCGCATC
CGCACCCGCCTGGTCACCGAAGCCGCCAGCCGCGACGAACTCCAAGCCTGCCTGCAACACCTGCTCGACACCGCCGGCAACGCCAGCCTCATGACCCCCG
CGCTCCAGCACACCCTCTGTGACCACGCCGTCGGCAACTACCGGATGCTCACCACCCTGGCCGCCGAACTGCTCGCCGTCGCCGCCCAACGCGACCTGCC
GCAGCTCGACGAAAAACTCTACCTCGACGTCTTCGCCCCCAACGAAAAACCCATCCCGCGCCGCGCCGCCGCACGCCGCTGAAACCCTCTGGAGACCCTC
ATGCGCTCATCCTCCTCTCCCCAATCGCTGCCCTGGCCCGAACTCAGCGACGCCTCCGTCGTCGCGTTGCAGGCCCTGTTCAGCGACTTCCTGGTCTTGT
TCGAATCCCACTACCTCGGGCAGATCCATCGCTACCACGACGAACGCTCGCAGGAAAACCTGACCCAACTGAGTTTCCTGTCCGACGATCCCATCCCACA
CGAAGACCCGGACAGCCCGCCGTTCTGAAGCTCTCCCAAGCCCTGCGCTCGAGACGCCGCCGAACCCCCTCCCGGGGTTCGGCGGCTTAGTGCTGAGCAG
ACCCCTCAACGAACGTGATCAGCGCAACATCAAATCAGGTGATCACACTCA

Protein section

ORF number : 4

ORF 1

Length		Begin	End	Strand	Fusion ORF
159 bp	52 aa	545	703	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MWQVLGARFLPPVAVVRLPAGLLERIPGQGQAPLLALLTWLWPLTVTAPEGR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1440 bp	479 aa	801	2240	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTTGNDPRHRDRWARLRFSIIGPLFAAPPQPGALQAALAALAERDWRHPVTGGPVRFGVSTLERWYYAARAATDPVAALRNRRRPKGHFMALTPVVIETL
VQQYREHPGWTAQLHFDNLCAAFAGCTEGMPSYATVRRYLKAHGMHRQARPRRDTEGAERARDRFERLEVRSYELEHVAALWHLDFHHGSRKVLTRDGTW
AKPYLLGILDDRSRLICHLQWYLDESAQGLVHGLSQAFMKRGLPRALMTDNGAAMLAEETVAGLQRLGVLHQTTLPYSPHQNGKQESLWGRIEGRLLPML
EGHTPLTLEVLNQATQAWAEQEYHRTHHSELGTTPLQRYLAGPNVRRDCPPVAALADAFRIEVTRRQRRSDGTVSLDGQRFEIPARYQHLDRVHLRYARW
DRSRVDLVDPHTGTLLCPVLPLDKSAHAHGQRRTRPPATPPLDPLPPSGMAPLLRQLLADYAATGLPPAYLPIPEEDDA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
846 bp	281 aa	2237	3082	+	No

ORF function : Passenger Gene
Annotation : General secretion pathway protein ADescription :

ORF sequence :

MNPKLLALYGLKWNPFATEIPIEALYVPARVEDFYWRIEQGLIREGGFAMVHGDPGTGKSAVLRLIAERLARLPDLTVAAIHHPQSHLADFYREMGDLFG
VPLRPHNRWGGFKALRENWFAHLDTTRRRAVLLIDEAQEMSPAVLSELRLMASARFDSQTLLCVVLAGDARLPEKLRREELIPLGSRIRTRLVTEAASRD
ELQACLQHLLDTAGNASLMTPALQHTLCDHAVGNYRMLTTLAAELLAVAAQRDLPQLDEKLYLDVFAPNEKPIPRRAAARR

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
228 bp	75 aa	3101	3328	+	No

ORF function : Passenger Gene
Annotation : Rhomboid family proteinDescription :

ORF sequence :

MRSSSSPQSLPWPELSDASVVALQALFSDFLVLFESHYLGQIHRYHDERSQENLTQLSFLSDDPIPHEDPDSPPF

Blast result :

Comments

ISTni2 is 44% aa (ORFB : the transposase) similar to ISChy6.
ORFA, ORFC and ORFD are passenger genes respectively annotated as hypothetical protein, General secretion pathway protein A and Rhomboid family protein.

References

1] ISfinder annotation (2013)
2] Tikhonova,T.V., Pavlov,A.R., Beletsky,A.V., Mardanov,A.V., Sorokin,D.Y., Ravin,N.V. and Popov,V.O. (2012) Direct submission GenBank.