ISNagr2

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003377 ND Natronobacterium gregoryi
Natronobacterium gregoryi SP2
DNA section
IS Length : 1067 bp

Ends


IR Length : 8/13

IRL : CTGATGGCTAAAACTTCATGTATCTCTAGATGCGGGGGAATAGTATGGCG
IRR : CTCAAGGACATAAGTACGTAAAGACATTCGGCGGTTCGATGTCGTCAAGC

Insertion site


Left flankDirect repeatRight flankDR Length
GATCCGTTCATATGAAGAGGCG2
TGCCTATTGATATCAATCGTCT2

DNA sequence

CTGATGGCTAAAACTTCATGTATCTCTAGATGCGGGGGAATAGTATGGCGACGTTTGAGAACGTCTCTGTCGAAGACCTCCGCCAGATCTTGGCGGAGGT
CGATGATGCCGATGCAACAAAGCGGCTCATGGCCGCGATCACCTACAAAGAGATAGAAGATCTAACGCAAGCAGAAGCCGCCGCACTCTATGGATTTTCC
AGCAGTTGGGCCTCGAAATGGTTCAATCGACTCGAACGGCTCGCCGACGAGCCGTTCGAGGAGGTCGTCTACGACAAACCACGAGAGGGCAGACCATCCG
AACTCTCTGACGACGAGCACGAGCAGTTTGTTGAGGTACTTCACGAGTCTCCCGAAGACGTTGGATACGACGCGCCCGCGTGGTCTGTTTCACTAGCGCG
TCACTATCTCTCCGAAGAATTCGGTGTTGAGTACTGCGAACGTCACGTCCGACGACTGATGTCCGAGGCCGGGCTGTCCTGGAAGACAGCCCGGCCGGAG
TTCCACAAGTCCGACGAACGAGCCCAAGAGGCCTGGGAAGAAGGGTTCAAAAAAAGCGCGACAACTTGGACGATGAATACACGATCCTGACCATCGATCA
GACGCGACAGGTTCTCTCGACGCTGATCTACGCGTGGTTTCCAGAAGGGGAGCGCCCGTCACTCCCGGTGACGGGCGCGTGGGACAGCATCAAACTCCTC
GGGGCAGTCAGCAATAGCGGCGAGACGTTCTTTCTCCCGTGTGAGGAGAATTTCAACAGCGACACCACAATCCGGTTGCTCGACGCTCTCCAAACCGAGT
TCGGCGAGAAAATCTGCGTTGTCTTAGATAATGCCTCCTATTTTACGGCGAACAAGGTGCAGGCATTCGCCGAAGGAACTCCGATCGAACTGTGTTACCT
TCCACGGGGTTCACCAGAGCTGAACCCCGTGGAAGAGTGCTGGCGACGACTCAATCAAGCACTCGGCAACCGCTTGTTCAACACACTGGATGAACTTCAA
GAGGCTGCACTGACCGCGCTTGACGACATCGAACCGCCGAATGTCTTTACGTACTTATGTCCTTGAG
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
546 bp181 aa45590+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MATFENVSVEDLRQILAEVDDADATKRLMAAITYKEIEDLTQAEAAALYGFSSSWASKWFNRLERLADEPFEEVVYDKPREGRPSELSDDEHEQFVEVLH
ESPEDVGYDAPAWSVSLARHYLSEEFGVEYCERHVRRLMSEAGLSWKTARPEFHKSDERAQEAWEEGFKKSATTWTMNTRS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
636 bp210 aa4311066+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VLRTSRPTTDVRGRAVLEDSPAGVPQVRRTSPRGLGRRVQKKRDNLDDEYTILTIDQTRQVLSTLIYAWFPEGERPSLPVTGAWDSIKLLGAVSNSGETF
FLPCEENFNSDTTIRLLDALQTEFGEKICVVLDNASYFTANKVQAFAEGTPIELCYLPRGSPELNPVEECWRRLNQALGNRLFNTLDELQEAALTALDDI
EPPNVFTYLC

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1022 bp340 aa451066+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MATFENVSVEDLRQILAEVDDADATKRLMAAITYKEIEDLTQAEAAALYGFSSSWASKWFNRLERLADEPFEEVVYDKPREGRPSELSDDEHEQFVEVLH
ESPEDVGYDAPAWSVSLARHYLSEEFGVEYCERHVRRLMSEAGLSWKTARPEFHKSDERAQEAWEEGFKKKRDNLDDEYTILTIDQTRQVLSTLIYAWFP
EGERPSLPVTGAWDSIKLLGAVSNSGETFFLPCEENFNSDTTIRLLDALQTEFGEKICVVLDNASYFTANKVQAFAEGTPIELCYLPRGSPELNPVEECW
RRLNQALGNRLFNTLDELQEAALTALDDIEPPNVFTYLCP

 

Blast result :
Comments
ISNagr2 is 92% aa similar to ISNamo15.
The third ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
References
1] Pfeiffer, F. (2013) Direct submission
2] Peters,L., Mikhailova,N., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank