ISNagr2
- Family IS630
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP003377 | ND | Natronobacterium gregoryi | Natronobacterium gregoryi SP2 |
DNA section
IS Length : 1067 bp
Ends
IR Length : 8/13
IRL : CTGATGGCTAAAACTTCATGTATCTCTAGATGCGGGGGAATAGTATGGCG
IRR : CTCAAGGACATAAGTACGTAAAGACATTCGGCGGTTCGATGTCGTCAAGC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GATCCGTTCA | TA | TGAAGAGGCG | 2 |
TGCCTATTGA | TA | TCAATCGTCT | 2 |
DNA sequence
CTGATGGCTAAAACTTCATGTATCTCTAGATGCGGGGGAATAGTATGGCGACGTTTGAGAACGTCTCTGTCGAAGACCTCCGCCAGATCTTGGCGGAGGT
CGATGATGCCGATGCAACAAAGCGGCTCATGGCCGCGATCACCTACAAAGAGATAGAAGATCTAACGCAAGCAGAAGCCGCCGCACTCTATGGATTTTCC
AGCAGTTGGGCCTCGAAATGGTTCAATCGACTCGAACGGCTCGCCGACGAGCCGTTCGAGGAGGTCGTCTACGACAAACCACGAGAGGGCAGACCATCCG
AACTCTCTGACGACGAGCACGAGCAGTTTGTTGAGGTACTTCACGAGTCTCCCGAAGACGTTGGATACGACGCGCCCGCGTGGTCTGTTTCACTAGCGCG
TCACTATCTCTCCGAAGAATTCGGTGTTGAGTACTGCGAACGTCACGTCCGACGACTGATGTCCGAGGCCGGGCTGTCCTGGAAGACAGCCCGGCCGGAG
TTCCACAAGTCCGACGAACGAGCCCAAGAGGCCTGGGAAGAAGGGTTCAAAAAAAGCGCGACAACTTGGACGATGAATACACGATCCTGACCATCGATCA
GACGCGACAGGTTCTCTCGACGCTGATCTACGCGTGGTTTCCAGAAGGGGAGCGCCCGTCACTCCCGGTGACGGGCGCGTGGGACAGCATCAAACTCCTC
GGGGCAGTCAGCAATAGCGGCGAGACGTTCTTTCTCCCGTGTGAGGAGAATTTCAACAGCGACACCACAATCCGGTTGCTCGACGCTCTCCAAACCGAGT
TCGGCGAGAAAATCTGCGTTGTCTTAGATAATGCCTCCTATTTTACGGCGAACAAGGTGCAGGCATTCGCCGAAGGAACTCCGATCGAACTGTGTTACCT
TCCACGGGGTTCACCAGAGCTGAACCCCGTGGAAGAGTGCTGGCGACGACTCAATCAAGCACTCGGCAACCGCTTGTTCAACACACTGGATGAACTTCAA
GAGGCTGCACTGACCGCGCTTGACGACATCGAACCGCCGAATGTCTTTACGTACTTATGTCCTTGAG
CGATGATGCCGATGCAACAAAGCGGCTCATGGCCGCGATCACCTACAAAGAGATAGAAGATCTAACGCAAGCAGAAGCCGCCGCACTCTATGGATTTTCC
AGCAGTTGGGCCTCGAAATGGTTCAATCGACTCGAACGGCTCGCCGACGAGCCGTTCGAGGAGGTCGTCTACGACAAACCACGAGAGGGCAGACCATCCG
AACTCTCTGACGACGAGCACGAGCAGTTTGTTGAGGTACTTCACGAGTCTCCCGAAGACGTTGGATACGACGCGCCCGCGTGGTCTGTTTCACTAGCGCG
TCACTATCTCTCCGAAGAATTCGGTGTTGAGTACTGCGAACGTCACGTCCGACGACTGATGTCCGAGGCCGGGCTGTCCTGGAAGACAGCCCGGCCGGAG
TTCCACAAGTCCGACGAACGAGCCCAAGAGGCCTGGGAAGAAGGGTTCAAAAAAAGCGCGACAACTTGGACGATGAATACACGATCCTGACCATCGATCA
GACGCGACAGGTTCTCTCGACGCTGATCTACGCGTGGTTTCCAGAAGGGGAGCGCCCGTCACTCCCGGTGACGGGCGCGTGGGACAGCATCAAACTCCTC
GGGGCAGTCAGCAATAGCGGCGAGACGTTCTTTCTCCCGTGTGAGGAGAATTTCAACAGCGACACCACAATCCGGTTGCTCGACGCTCTCCAAACCGAGT
TCGGCGAGAAAATCTGCGTTGTCTTAGATAATGCCTCCTATTTTACGGCGAACAAGGTGCAGGCATTCGCCGAAGGAACTCCGATCGAACTGTGTTACCT
TCCACGGGGTTCACCAGAGCTGAACCCCGTGGAAGAGTGCTGGCGACGACTCAATCAAGCACTCGGCAACCGCTTGTTCAACACACTGGATGAACTTCAA
GAGGCTGCACTGACCGCGCTTGACGACATCGAACCGCCGAATGTCTTTACGTACTTATGTCCTTGAG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
546 bp | 181 aa | 45 | 590 | + | No |
Description : First part of the transposase
ORF sequence :
MATFENVSVEDLRQILAEVDDADATKRLMAAITYKEIEDLTQAEAAALYGFSSSWASKWFNRLERLADEPFEEVVYDKPREGRPSELSDDEHEQFVEVLH
ESPEDVGYDAPAWSVSLARHYLSEEFGVEYCERHVRRLMSEAGLSWKTARPEFHKSDERAQEAWEEGFKKSATTWTMNTRS
ESPEDVGYDAPAWSVSLARHYLSEEFGVEYCERHVRRLMSEAGLSWKTARPEFHKSDERAQEAWEEGFKKSATTWTMNTRS
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
636 bp | 210 aa | 431 | 1066 | + | No |
Description : Second part of the transposase
ORF sequence :
VLRTSRPTTDVRGRAVLEDSPAGVPQVRRTSPRGLGRRVQKKRDNLDDEYTILTIDQTRQVLSTLIYAWFPEGERPSLPVTGAWDSIKLLGAVSNSGETF
FLPCEENFNSDTTIRLLDALQTEFGEKICVVLDNASYFTANKVQAFAEGTPIELCYLPRGSPELNPVEECWRRLNQALGNRLFNTLDELQEAALTALDDI
EPPNVFTYLC
FLPCEENFNSDTTIRLLDALQTEFGEKICVVLDNASYFTANKVQAFAEGTPIELCYLPRGSPELNPVEECWRRLNQALGNRLFNTLDELQEAALTALDDI
EPPNVFTYLC
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1022 bp | 340 aa | 45 | 1066 | + | Yes |
Chemistry : DDE
ORF sequence :
MATFENVSVEDLRQILAEVDDADATKRLMAAITYKEIEDLTQAEAAALYGFSSSWASKWFNRLERLADEPFEEVVYDKPREGRPSELSDDEHEQFVEVLH
ESPEDVGYDAPAWSVSLARHYLSEEFGVEYCERHVRRLMSEAGLSWKTARPEFHKSDERAQEAWEEGFKKKRDNLDDEYTILTIDQTRQVLSTLIYAWFP
EGERPSLPVTGAWDSIKLLGAVSNSGETFFLPCEENFNSDTTIRLLDALQTEFGEKICVVLDNASYFTANKVQAFAEGTPIELCYLPRGSPELNPVEECW
RRLNQALGNRLFNTLDELQEAALTALDDIEPPNVFTYLCP
ESPEDVGYDAPAWSVSLARHYLSEEFGVEYCERHVRRLMSEAGLSWKTARPEFHKSDERAQEAWEEGFKKKRDNLDDEYTILTIDQTRQVLSTLIYAWFP
EGERPSLPVTGAWDSIKLLGAVSNSGETFFLPCEENFNSDTTIRLLDALQTEFGEKICVVLDNASYFTANKVQAFAEGTPIELCYLPRGSPELNPVEECW
RRLNQALGNRLFNTLDELQEAALTALDDIEPPNVFTYLCP
Blast result :
Comments
ISNagr2 is 92% aa similar to ISNamo15.
The third ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
The third ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
References
1] Pfeiffer, F. (2013) Direct submission
2] Peters,L., Mikhailova,N., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank
2] Peters,L., Mikhailova,N., Teshima,H., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Sproer,C., Anderson,I. and Woyke,T. (2012) Direct submission GenBank