ISNamo16
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HF582854 | ND | Natronomonas moolapensis | Natronomonas moolapensis 8.8.11 |
DNA section
IS Length : 1058 bp
Ends
IR Length : 12/15
IRL : TAGTAGTCGGTAGAACTTTTTACTCGTGGCTTGTTGCAGTATGTAGTGAA
IRR : TAGTAGACACTAGAAGTAATTGCTCACAGTAGGAACAGAGAGTTTGTCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GAAGAAGAGC | TA | GTGTGATAAA | 2 |
DNA sequence
TAGTAGTCGGTAGAACTTTTTACTCGTGGCTTGTTGCAGTATGTAGTGAATCATCTCGACGAGATCTCCGTTGAGGAATTACAAGACGCCCTCGACAACG
TGGACGAAAAGAAGCCGACACAACGGCTCTTAGCCGCAATCGCGTACAAAAACGGTGTGTCGCAAACTGAGTTAGCCGAGTGGTACGACGTTCAGCGGCG
GACGATCTACAGCTGGCTCAAGCGACTCGACACCGACGAGTCGCTTGGGCAGGCCGTGTCTGATGCTAAACGAACCGGGAGAAAACGAAAGTTGTCAGAA
TCACAGCTAAAAGAATTTGAAGAAACTGTACACGAACCACCCGACGAGGTTGGTATTGACGCGCCGGCGTGGACGCCGGCGCTCGCGCAAAACTTTCTCG
AAGAGACCTACGGCGTCGAGTATTCAGTCTCGTCTTGTCGGCGGTTGCTGAAAGAGGCTGGGTTGAGTCATCAAAAACCCCGCCGAACAGCCGCTGAAAC
CGATGAAGACGAGCAAGACCGGTTCCACGACGAGCTCAAAAAAAAGCGACGGGAGCTGGACGCCACGGTAGTTTGTATCGATCAAACAAAAAAAAATCCG
TGCAAGTCGAGCCGCGTGCCGCGTGGTTTCCGCGCGGCACGCGGCCCTCGGTCGAACTGTCTGGTCAACGTGATTGGACGTGTCTGTTGGGTGCGATCAC
CGAGGACGGTGATCGCTTCTTCTCTCGGTTTACCGAGTACGTTACGGCTGAACACGCGAAACATTTCATTTTAGCATTATGCAAAGAATTTGAAGATGAT
TTGATTGTTGTGCTGGACGGAGCGCCGTATTTTCAGGCGTCAGCCGTCACGGACCTGGCGGCCCGTGACGACCTCACCTTCGTGACGTTACCGTCGTATT
CACCGGAATTAAATCCGGTCGAAGAATGCTGGAGACAGGTACAAAAGGCACTTAGCAACCGATTCTTTGACTCTCTCGACGAGCTAACAACGGCGATTGA
CACCGCTCTCGACAAACTCTCTGTTCCTACTGTGAGCAATTACTTCTAGTGTCTACTA
TGGACGAAAAGAAGCCGACACAACGGCTCTTAGCCGCAATCGCGTACAAAAACGGTGTGTCGCAAACTGAGTTAGCCGAGTGGTACGACGTTCAGCGGCG
GACGATCTACAGCTGGCTCAAGCGACTCGACACCGACGAGTCGCTTGGGCAGGCCGTGTCTGATGCTAAACGAACCGGGAGAAAACGAAAGTTGTCAGAA
TCACAGCTAAAAGAATTTGAAGAAACTGTACACGAACCACCCGACGAGGTTGGTATTGACGCGCCGGCGTGGACGCCGGCGCTCGCGCAAAACTTTCTCG
AAGAGACCTACGGCGTCGAGTATTCAGTCTCGTCTTGTCGGCGGTTGCTGAAAGAGGCTGGGTTGAGTCATCAAAAACCCCGCCGAACAGCCGCTGAAAC
CGATGAAGACGAGCAAGACCGGTTCCACGACGAGCTCAAAAAAAAGCGACGGGAGCTGGACGCCACGGTAGTTTGTATCGATCAAACAAAAAAAAATCCG
TGCAAGTCGAGCCGCGTGCCGCGTGGTTTCCGCGCGGCACGCGGCCCTCGGTCGAACTGTCTGGTCAACGTGATTGGACGTGTCTGTTGGGTGCGATCAC
CGAGGACGGTGATCGCTTCTTCTCTCGGTTTACCGAGTACGTTACGGCTGAACACGCGAAACATTTCATTTTAGCATTATGCAAAGAATTTGAAGATGAT
TTGATTGTTGTGCTGGACGGAGCGCCGTATTTTCAGGCGTCAGCCGTCACGGACCTGGCGGCCCGTGACGACCTCACCTTCGTGACGTTACCGTCGTATT
CACCGGAATTAAATCCGGTCGAAGAATGCTGGAGACAGGTACAAAAGGCACTTAGCAACCGATTCTTTGACTCTCTCGACGAGCTAACAACGGCGATTGA
CACCGCTCTCGACAAACTCTCTGTTCCTACTGTGAGCAATTACTTCTAGTGTCTACTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 525 bp | 174 aa | 46 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
MDHLDEISVEELHDALNNVEGRKPTQRLIAAIEYKNGVTQTELAEWHNTGRRTIYSWLKRLDTDESLEQAVTDAHRSGRKRKLSEKEQQEFEDTVHESPE
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKSGGKWTPR
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKSGGKWTPR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 537 bp | 178 aa | 510 | 1046 | + | No |
Description : Second part of the transposase
ORF sequence :
GPRNVPRRTQKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRPSVELSGQRDWTCLLGAITENGDRFFTRFEEYVTAAHAKHFILALCQEFEDNLIVV
LDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQAALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
LDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQAALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1001 bp | 333 aa | 46 | 1046 | + | Yes |
Chemistry : DDE
ORF sequence :
MDHLDEISVEELHDALNNVEGRKPTQRLIAAIEYKNGVTQTELAEWHNTGRRTIYSWLKRLDTDESLEQAVTDAHRSGRKRKLSEKEQQEFEDTVHESPE
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITENGDRFFTRFEEYVTAAHAKHFILALCQEFEDNLIVVLDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQA
ALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
EVGVDAPAWTPALVQQYLDETYDVEYSIPSCRRLLKEAGLSYQKPRPTAAESDAEDQETFREELKKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITENGDRFFTRFEEYVTAAHAKHFILALCQEFEDNLIVVLDGAPYFQASAVTDLAARDDLTFVRLPAYSPELNPVEECWRQLQA
ALSNRFFDSLPELTTAIDTALDQLSLPKVSNHF
Blast result :
Comments
ISNamo16 is 91% aa similar to ISNpe3.
The ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
The ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.
References
1] Pfeiffer, F. (2013) Direct submission
2] Dyall-Smith, M.L., Pfeiffer, F., Oberwinkler, T., Klee, K., Rampp, M., Palm, P., Gross, K., Schuster, S.C., Oesterhelt, D. (2013) Genome Announc. 1: e0009513
2] Dyall-Smith, M.L., Pfeiffer, F., Oberwinkler, T., Klee, K., Rampp, M., Palm, P., Gross, K., Schuster, S.C., Oesterhelt, D. (2013) Genome Announc. 1: e0009513