ISfinder Mirror Site

ISNamo15

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
HF582854	ND	Natronomonas moolapensis	Natronomonas moolapensis 8.8.11

DNA section

IS Length : 1066 bp

Ends

IR Length : 7/13

IRL : CTGAGGGCTAAAAATTCATGTAACTCCAGCCACGAGAAACGGTATGGCGA
IRR : CTCACGGACATAAGTACGTAAAGACATCTGGAACGGTAATCCGGTTGAGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAAATGGCTTA	TA	TTAATAAGCGG	2
TACAAGACTCA	TA	TCAACTATGAT	2
AGCCGGATTCA	TA	TCAGTCGGCGG	2

DNA sequence

CTGAGGGCTAAAAATTCATGTAACTCCAGCCACGAGAAACGGTATGGCGAGGCTGGAGAAGGTCTCTGTCGAAGACCTCCGCCAGATCCTGGCGGAGGTC
GATGAAGCCGACGCAGCAAAGCGGATTATGGCTGCGATTACCTACAAGGAGATCGACGATCTGACGCAGACAGACGCTGCCGAACTCTACGGATTTTCCA
GTAGTTGGGCCTCAAAGTGGTTCACCCGACTCGAACGGCTCGCCGACGAGCCGTTCGAGGAGGTTGTCTATGATAAACCACGAGAAGGCAGGCCGTCCGA
GCTTTCTGACAAAGAACACGACCAGTTCGTCGAGGCTCTTCACGACTCACCCGGAGAAGTCAGATACGACGCACCCGCGTGGTCTGTTCCATTGGCGCGT
CACTATCTCTCCGAGGAGTTCGGTGTTGAGTACTGCGAACGTCACGTCCGACGACTGATCTCCGAGGCCGGGCTGTCCTGGAAGACAGCCCGGCCGGAGT
TCTACAAATCCGACGAGAGAGCCCAAGAAGCCTGGCAGGAAGGGTTCAAAAAAAGCGCGACAACTTGGACGATGAATACACGATCCTGACCATCGATCAG
ACGCGTCAGGTACTCTCGACGCTGATCTATGCGTGGTTTCCGGAAGGGGAGCGCCCGTCACTTCCGGTGACGGGCGCGTGGGATAGCATCAAGTTGCTCG
GTGCAGTCAGCGATTCTAGGGAGACGTTCTTTCTGCCGTGTGCAGAGAACTTCAACAGCGACACGACGATTCGCTTGCTAGACGCACTCCAGACAGAGTT
CGGCAAGAAAATCTGCGTCATCTTGGACAACGCGTCGTATTTCACGGCTAACGCAGTCCAGGAGTTCGTCGAAGACACGCCGATCGAACTATGCTACCTT
CCGCGGGGTTCACCGGAGCTGAACCCCGCGGAAGAGTGCTGGCGACAACTCGATCAAGAACTTGGCAACCGTCTGTTCGACACACTCGACGAACTTCGTG
ATGCTGCGCTCTGTGCACTCAACCGGATTACCGTTCCAGATGTCTTTACGTACTTATGTCCGTGAG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
546 bp	181 aa	44	589	+	No

ORF function : Transposase
Description :

ORF sequence :

MARLEKVSVEDLRQILAEVDEADAAKRIMAAITYKEIDDLTQTDAAELYGFSSSWASKWFTRLERLADEPFEEVVYDKPREGRPSELSDKEHDQFVEALH
DSPGEVRYDAPAWSVPLARHYLSEEFGVEYCERHVRRLISEAGLSWKTARPEFYKSDERAQEAWQEGFKKSATTWTMNTRS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
636 bp	211 aa	430	1065	+	No

ORF function : Transposase
Description :

ORF sequence :

VLRTSRPTTDLRGRAVLEDSPAGVLQIRRESPRSLAGRVQKKRDNLDDEYTILTIDQTRQVLSTLIYAWFPEGERPSLPVTGAWDSIKLLGAVSDSRETF
FLPCAENFNSDTTIRLLDALQTEFGKKICVILDNASYFTANAVQEFVEDTPIELCYLPRGSPELNPAEECWRQLDQELGNRLFDTLDELRDAALCALNRI
TVPDVFTYLCP

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1022 bp	340 aa	44	1065	+	Yes

ORF function : Transposase
Description :

ORF sequence :

MARLEKVSVEDLRQILAEVDEADAAKRIMAAITYKEIDDLTQTDAAELYGFSSSWASKWFTRLERLADEPFEEVVYDKPREGRPSELSDKEHDQFVEALH
DSPGEVRYDAPAWSVPLARHYLSEEFGVEYCERHVRRLISEAGLSWKTARPEFYKSDERAQEAWQEGFKKKRDNLDDEYTILTIDQTRQVLSTLIYAWFP
EGERPSLPVTGAWDSIKLLGAVSDSRETFFLPCAENFNSDTTIRLLDALQTEFGKKICVILDNASYFTANAVQEFVEDTPIELCYLPRGSPELNPAEECW
RQLDQELGNRLFDTLDELRDAALCALNRITVPDVFTYLCP

Blast result :

Comments

ISNma15 is 92% aa similar to ISNagr2.
IS_pep_1 is Nmlp_1628, Nmlp_2612, and Nmlp_3157.
The ORF is an ORFAB transposase reconstructed in silico by a -1 frameshift probably reflecting ribosomal slippage.

References

1] Pfeiffer, F. (2013) Direct submission
2] Dyall-Smith, M.L., Pfeiffer, F., Oberwinkler, T., Klee, K., Rampp, M., Palm, P., Gross, K., Schuster, S.C., Oesterhelt, D. (2013) Genome Announc. 1: e0009513