ISNamo14

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
HF582854 ND Natronomonas moolapensis
Natronomonas moolapensis 8.8.11
DNA section
IS Length : 1067 bp

Ends


IR Length : 8/9

IRL : TACTGACGGCTAAACTTATTTGTGTATAGAGCTTGTTTTTACGGTATGGA
IRR : TACTCACGGACAGAGATAATTATAGATGTTTGGTGGGTCTATAGACGCAA

Insertion site


Left flankDirect repeatRight flankDR Length
CAACTGTGCATAATTGGGTTCA2
GGGGTCAAAATATGCTACTACA2
CTTTGGGTAATATACTGGTCGA2
TTCTGGGTCATATGTTATACCA2
CATCCACAGCTAATTTATTCTG2
GGTCATATAATATGAACGAGCA2

DNA sequence

TACTGACGGCTAAACTTATTTGTGTATAGAGCTTGTTTTTACGGTATGGACTACTTGGAGAAGATCACTGTTGACGAACTCCAAGAGATTTTAGCCGAAG
TTGATGAAAAGAAACCAGTTCAGAGAATTCTTGCCGGTATCGCCTACAAGGACGGTGTCGAGCAGCAGACAATTGCGGAGCGGCATGATGTCCATCCAAA
CACGGTTCGTAACTGGCTGATCCGACTCGAACGGCTCGACTCAGAGCCGTTCGAGTCGGTCGTCTACGATGATCCTCGCCCCGGCCAATCACGCGAACTT
GACGAAGCTGACCACGACCAATTCATCGAAGCTCTCCACGATTCTCCCAAAGAAGTTGGACTTGATGCGCGTGCGTGGACGGTTCCGCTAGCCCAGCAGT
ATCTTGAAGATGAGTTCGATATTGAGTACTGCCGCCGTCATATCCGGCGATTGATGTCCGAGGCCGGGCTGTCCTGGAAGACAGCCCGGCCAGAGTACGT
CAAAGCTGATGAACGAGCTCAGGATGCGTTCCGCAAAGGCTTCAAAAAAAGACGGACGATCTGGACGACGACTACACAATCATAGCAATCGACCAGACAC
GCCAAGAGATCACGACGGATCTTGTCCACGCGTGGTTTCCAGAAAAAACGCGCCCGACACTCCCGGTGTCGGGCGCGTGGGAGAGTCTCAAACTGCTGGG
CGGTGTCACCGCCAGCGGTGACACCTTCTTTCTGAAGTGTGAAGACAACTTTACCGCTGACATCACGACGCGGTTACTGGACGCACTCCAGACCGAGTTC
GGCGAGAAAATCTGTGTTGTCCTGGACAATGCCCCGTATTTCGCGGCGAACGATGTCCACGACTACGTTGAAGACACACCGATCGAACTGTGCCACCTTC
CGCGGGGTTCACCGGAGCTGAACCCCGCGGAAGGGTGCTGGCAGAAACTCAATCAGCGGCTTGGTAACCAACTTTTCGAAGAACTTGATGAACTGAGAGA
AGCCGTCTTCGATGCGCTTGCGTCTATAGACCCACCAAACATCTATAATTATCTCTGTCCGTGAGTA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
540 bp179 aa46585+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDYLEKITVDELQEILAEVDEKKPVQRILAGIAYKDGVEQQTIAERHDVHPNTVRNWLIRLERLDSEPFESVVYDDPRPGQSRELDEADHDQFIEALHDS
PKEVGLDARAWTVPLAQQYLEDEFDIEYCRRHIRRLMSEAGLSWKTARPEYVKADERAQDAFRKGFKKRRTIWTTTTQS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
552 bp183 aa5131064+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TSSGCVPQRLQKKTDDLDDDYTIIAIDQTRQEITTDLVHAWFPEKTRPTLPVSGAWESLKLLGGVTASGDTFFLKCEDNFTADITTRLLDALQTEFGEKI
CVVLDNAPYFAANDVHDYVEDTPIELCHLPRGSPELNPAEGCWQKLNQRLGNQLFEELDELREAVFDALASIDPPNIYNYLCP

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1019 bp339 aa461064+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDYLEKITVDELQEILAEVDEKKPVQRILAGIAYKDGVEQQTIAERHDVHPNTVRNWLIRLERLDSEPFESVVYDDPRPGQSRELDEADHDQFIEALHDS
PKEVGLDARAWTVPLAQQYLEDEFDIEYCRRHIRRLMSEAGLSWKTARPEYVKADERAQDAFRKGFKKKTDDLDDDYTIIAIDQTRQEITTDLVHAWFPE
KTRPTLPVSGAWESLKLLGGVTASGDTFFLKCEDNFTADITTRLLDALQTEFGEKICVVLDNAPYFAANDVHDYVEDTPIELCHLPRGSPELNPAEGCWQ
KLNQRLGNQLFEELDELREAVFDALASIDPPNIYNYLCP

 

Blast result :
Comments
ISNamo14 is 60% aa similar to ISHvo16.
The ORF is an ORFAB transposase reconstructed in silico by a +1 frameshift probably reflecting ribosomal slippage.
References
1] Pfeiffer, F. (2013) Direct submission
2] Dyall-Smith, M.L., Pfeiffer, F., Oberwinkler, T., Klee, K., Rampp, M., Palm, P., Gross, K., Schuster, S.C., Oesterhelt, D. (2013) Genome Announc. 1: e0009513