ISNma7

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001934 ND Natrialba magadii
Natrialba magadii ATCC 43099 plasmid pNMAG02
DNA section
IS Length : 1059 bp

Ends


IR Length : 12/14

IRL : TAGTAACCGTTAGATCTTTCCGCCCATAGATTGTTGCTACTTCTAATGGA
IRR : TAGTAGACGTTAGAAATAATTGCTCACTTTTGGGACGGTGATCTGATCAA

Insertion site


Left flankDirect repeatRight flankDR Length
AACAACTGCAACTAGTTACACACTGA2

DNA sequence

TAGTAACCGTTAGATCTTTCCGCCCATAGATTGTTGCTACTTCTAATGGATCATCTCGACGAGATCTCCGTCGAAGAACTTCAAGACGCCCTTGATAGGG
TTGAGGGAGCTAAGCCGACACAACGATTGTTAGCGGCGATTGCGTACAAGAACGGCGTGAATCAGACCGAACTTGCGGAATGGCACGACACTGGTCGACG
AACAATCTACAGTTGGCTCATGCGACTCGATACGGACGAACCGCTTGAGCAAGCCGTTTCTGATGCTCATCGATCCGGGAGAAAACGAAAGCTCTCAGAA
AAAGAGCAACAAGAGTTCGAAGAAACAGTCCACAGGTCTCCTGAAGAAGTTGGTTTTGACGCGCCGGCGTGGACGCCGGCGCTTGTCCAGCAGCATCTCG
CCGAGACCTACGATGTCGAGTACTCAATCCCAAGCTGCCGGCGGTTGCTCAAAGAAGCGGGATTGAGCTATCAAAAACCACGCCGTACAGCCGCCGAATC
TGAGGCTGAGGAACAAGAAGCGTTCCGCGAAGAACTCAAAAAAAGCGGCGGGAAATGGACGCCACAGTAGTCTGTATCGATCAAACCAAGAAATCCGTCC
AAGTTGAGCCGCGTGCCGCGTGGTTTCCTCGCGGCACGCGACCGTCTGTTGAATTGTCCGGACAACGCGACTGGACGTGTCTCTTAGGTGCGATCACCGA
GGACGGTGATCGCTTTTTCGCTCGATTCGAAGAGTATGTAACGGCTGATCACGCAAAACACTTCATTCTTGCATCATGTAATGAGTTCGAAGAGGACTTG
ATTATTGTGTTGGATGGAGCGCCGTATTTTCGGGCGTCGGCCGTCACGGACCTGGCGGCCCGTGACGACCTCGCCTTCGTGACATTGCCGGCGTACTCGC
CAGAGCTGAATCCCGTCGAAGAGTGCTGGAGACAGCTCCAAGCGGCACTCAGCAATCGTTTCTTTGGCTCACTCAACGAGCTTACAACAGCGATCGATAC
AGCTATTGATCAGATCACCGTCCCAAAAGTGAGCAATTATTTCTAACGTCTACTA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
525 bp174 aa46570+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDHLDEISVEELQDALDRVEGAKPTQRLLAAIAYKNGVNQTELAEWHDTGRRTIYSWLMRLDTDEPLEQAVSDAHRSGRKRKLSEKEQQEFEETVHRSPE
EVGFDAPAWTPALVQQHLAETYDVEYSIPSCRRLLKEAGLSYQKPRRTAAESEAEEQEAFREELKKSGGKWTPQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
537 bp178 aa5101046+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GTRSVPRRTQKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRPSVELSGQRDWTCLLGAITEDGDRFFARFEEYVTADHAKHFILASCNEFEEDLIIV
LDGAPYFRASAVTDLAARDDLAFVTLPAYSPELNPVEECWRQLQAALSNRFFGSLNELTTAIDTAIDQITVPKVSNYF

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1001 bp333 aa461046+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDHLDEISVEELQDALDRVEGAKPTQRLLAAIAYKNGVNQTELAEWHDTGRRTIYSWLMRLDTDEPLEQAVSDAHRSGRKRKLSEKEQQEFEETVHRSPE
EVGFDAPAWTPALVQQHLAETYDVEYSIPSCRRLLKEAGLSYQKPRRTAAESEAEEQEAFREELKKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITEDGDRFFARFEEYVTADHAKHFILASCNEFEEDLIIVLDGAPYFRASAVTDLAARDDLAFVTLPAYSPELNPVEECWRQLQA
ALSNRFFGSLNELTTAIDTAIDQITVPKVSNYF

 

Blast result :
Comments
ISNma7 is 92% aa similar to ISNma6.
The ORF is an ORFAB transposase reconstructed in silico by a +1 frameshift probably reflecting ribosomal slippage.
References
1] Pfeiffer, F. (2013) Direct submission
2] Siddaramappa, S., Challacombe, J.F., Decastro, R.E., Pfeiffer, F., Sastre, D.E., Gimenez, M.I., Paggi, R.A., Detter, J.C., Davenport, K.W., Goodwin, L.A., Kyrpides, N., Tapia, R., Pitluck, S., Lucas, S., Woyke, T., Maupin-Furlow, J.A. (2012) BMC Genomics 13: 165