ISGlo6

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001090 ND Geobacter lovleyi
Geobacter lovleyi SZ plasmid pGLOV01
DNA section
IS Length : 1269 bp

Ends


IR Length : 19/27

IRL : TACTCACGCTTTTTCGGACAGGTAGTTAAGTTTGGTTTGCCGTAGATTAC
IRR : TACTCACGGTTGTCAAGACAGCAAATTGAAGATTTTAAGTTTGGTCGTGC

Insertion site


Left flankDirect repeatRight flankDR Length
CGCTCTATAAGCTCAGTGTG0

DNA sequence

TACTCACGCTTTTTCGGACAGGTAGTTAAGTTTGGTTTGCCGTAGATTACGGGGGTAATCTATGATGGCGACCATGCGAACAAAGCGGAGTCCTGAGTTC
AAGGCCAAGATAGCCTTGGCGGCTTTACGTGGCGACAAGACGATAAACGAGCTTGCCACCCTCTACAGTGTACACCCGAATCAGATCACGGCCTGGAAGC
GTCAGGCCCAGGAACATCTGGTTGCGGTCTTTGACAGTAAGAAAGCCAAATCCTCCAAGGCTGATGAAAAGCTGCAGGAGGAACTCTATCGCCAGATAGG
CCAGCTCAAGGTTGAACTTGACTGGCTCAAAAAAAAATCTGGCTTGTAGCACCGCCCGCAAGCGTGAGCTGATCGACTGGCGTCATCCAACGATCAGTAT
TGCCCGGCAGTGTGAGCTGCTTGGAGTAAGCCGCTCTTGCCTATACTATCATCCGGTTCCGGCTTCCAACGAGAACCGGCTTTTGATGCGGCTACTTGAT
GAGGAATACACCCGGCATCCGTTTCTGGGTGTCATCAAGCTCACCAACTGGTTAAGGAGCCAGGGATATTGGCATATAGGCACCCGCAGGGTACGGCGAC
TGTTACGGCTCATGGGCCTGATGGCCATCTATCCCGGCCCGAATCTAAGCAAACCGGCGCCGGGCCACAAGATCTATCCCTATCTGCTGCGCAACGTTGA
GGTAGAGCGGGTTAACCAAGTCTGGAGTGCCGATATAACGTACATTCGGCTCAAGACCGGCTTTGTCTATCTGGTAGCCGTTGTCGACTGGTGCAGCCGG
TACATCCTGGCCTTTGAAATCAGCATCACTCTGGAGGCCGACTTCTGCATCGAAGCGCTCCAGCAGGCGTTAACCAGAGGTACGCCGGAGATATTCAACA
GTGATCAGGGCTCTCAATTCACAAGCCCTCGTCACACGGAAATTCTGCACTTGGCTGGTGTCAGAATCAGCATGGATGGCAAAGGCAGAGCCCTCGACAA
CATTTTTGTCGAACGATTCTGGCGCTCGGTCAAATACGAGGAGGTCTACCTCCATGATTACGAATCAGTGCAAGAGGCCAGAATCGGGTTAAAGCGCTAC
ATCGAGTACTACAACAACGAACGGCAGCATCAATCACTGGGCTACCAGACACCGGCTGAAGTCTACTTCAAAGGTCTGCTCAATGAAGTTGACCAACTGA
AAAACAGGGACAACAACAGGCACGACCAAACTTAAAATCTTCAATTTGCTGTCTTGACAACCGTGAGTA
Recoding section
  • Recoding by frameshift
  • Frame +1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
288 bp95 aa62349+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MMATMRTKRSPEFKAKIALAALRGDKTINELATLYSVHPNQITAWKRQAQEHLVAVFDSKKAKSSKADEKLQEELYRQIGQLKVELDWLKKKSGL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
936 bp311 aa3001235+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MRLLDEEYTRHPFLGVIKLTNWLRSQGYWHIGTRRVRRLLRLMGLMAIYPGPNLSKPAPGHKIYPYLLRNVEVERVNQVWSADITYIRLKTGFVYLVAVV
DWCSRYILAFEISITLEADFCIEALQQALTRGTPEIFNSDQGSQFTSPRHTEILHLAGVRISMDGKGRALDNIFVERFWRSVKYEEVYLHDYESVQEARI
GLKRYIEYYNNERQHQSLGYQTPAEVYFKGLLNEVDQLKNRDNNRHDQT

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1174 bp390 aa621235+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MMATMRTKRSPEFKAKIALAALRGDKTINELATLYSVHPNQITAWKRQAQEHLVAVFDSKKAKSSKADEKLQEELYRQIGQLKVELDWLKKNLACSTARK
RELIDWRHPTISIARQCELLGVSRSCLYYHPVPASNENRLLMRLLDEEYTRHPFLGVIKLTNWLRSQGYWHIGTRRVRRLLRLMGLMAIYPGPNLSKPAP
GHKIYPYLLRNVEVERVNQVWSADITYIRLKTGFVYLVAVVDWCSRYILAFEISITLEADFCIEALQQALTRGTPEIFNSDQGSQFTSPRHTEILHLAGV
RISMDGKGRALDNIFVERFWRSVKYEEVYLHDYESVQEARIGLKRYIEYYNNERQHQSLGYQTPAEVYFKGLLNEVDQLKNRDNNRHDQT

 

Blast result :
Comments
ISGlo6 is 71% aa similar to ISPpy1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible +1 frameshift.
This IS has atypic IRs: it begins with 'TA' instead of 'TG'.
References
1] Mayya A. Petrova (2013) Direct submission to ISfinder
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Meincke,L., Brettin,T., Detter,J.C., Han,C., Tapia,R., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Sung,Y., Fletcher,K.E., Ritalahti,K.M., Loeffler,F.E. and Richardson,P. (2008) Direct submission GenBank