ISCysp23
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000807 | ND | Cyanothece sp. | Cyanothece sp. ATCC 51142 |
DNA section
IS Length : 1283 bp
Ends
IR Length : 15/22
IRL : TGTACCCCAGAAACTGTACAAAGGGTTAAGATAGAAATGCTGCTCCTAAA
IRR : TGTACCCAATTGTCTAGACAAATAACTAATAAAACTAAGATAGAAAGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATGGCTATAA | CTTCAGGTGA | 0 |
DNA sequence
TGTACCCCAGAAACTGTACAAAGGGTTAAGATAGAAATGCTGCTCCTAAACTATCAACAACAAGAGAAAAAAATGCAGCGTAAAAGATATAGTGCAGAGT
TTAAAGCTAAAATTGCTCTAGAAGCCATAAAAGGGCTTAAAACTGTCAATGAAATTGCAGGAGAGTTTGGGGTGCATCCTACCCAAATTCAACAATGGAA
AAAGCAACTTCTTGAACGATTACCAGACATCTTTAGTCAAAAACAAGAGAAACGAAAAAAACAAGATGAAGAGCTAACAGCCCAATTATATCAACAAATC
GGTCAACTCAAAGTAGAGCTTGATTGGGTGAAAAAAAAAGCTGGACTGCTCGGTAAGTGATAAACGGACACTAATCGAACCCGAACATTCACAGATTAAT
CTGTCTCGCCAGTGTGAATTACTGGGATTAGCACGTTCTAGCTACTATTACGAGTCAACAGGAGAAAGTGAACTTAATCTAGAACTAATGAAACTAATTG
ACCAACAATATACTCAAACGCCTTTTTACGGCATTAGACGCATGACAGCTTGCCTTCAAAAGCAAGGTTATCAAGTTAATCATAAACGAGTAGCTCGTTT
GATGAGAAAAATGGGGTTAGAAACCATTTATCCGCGTCCAAAGCTAAGCCAGTCCGATGATAATCATCGTCGCTATCCTTATCTACTTAACGATCTGACC
ATTGATCAACCGAACCAAGTTTGGTGTAGCGACATTACCTATATCCGAATGCCCAAAGGATTCGTCTACTTGGTGGCCATCATGGATTGGTATTCTCGCT
TTGTGCTGGCATGGCAGTTATCCAATACTCTCGAAGTCAGTTTTTGTCTACAAGCGTTGGCGATCGCAGCTAAATGGGGTAAGCCTGAGATTTTTAATTC
AGATCAAGGCTCTCAATATACCAGTCGTGAATTTGTGGACTCCTTAGAAAATCTGGGAATTACCATTAGTCAAGATGGAAGAGGCCGAGTCTTTGATAAC
ATCTTTATTGAACGTTTATGGCGTTCTGTAAAGTACGAAGAAGTTTATCTCAAAGATTATTCTACTGTAGCGATCGCCTTAGAAAACCTGAGCAACTACT
TTGAGTTTTATAACTATCGTAGGTTGCATCAATCTTTGGGTTATCAATGTCCAGCTACAATACATTTTCAAGATTAAGGAGGTGATTTAATTAAATTAAC
AAAAGTCTAAAATCTAGTCAGGTCAAAAGCTAACAGCTTTCTATCTTAGTTTTATTAGTTATTTGTCTAGACAATTGGGTACA
TTAAAGCTAAAATTGCTCTAGAAGCCATAAAAGGGCTTAAAACTGTCAATGAAATTGCAGGAGAGTTTGGGGTGCATCCTACCCAAATTCAACAATGGAA
AAAGCAACTTCTTGAACGATTACCAGACATCTTTAGTCAAAAACAAGAGAAACGAAAAAAACAAGATGAAGAGCTAACAGCCCAATTATATCAACAAATC
GGTCAACTCAAAGTAGAGCTTGATTGGGTGAAAAAAAAAGCTGGACTGCTCGGTAAGTGATAAACGGACACTAATCGAACCCGAACATTCACAGATTAAT
CTGTCTCGCCAGTGTGAATTACTGGGATTAGCACGTTCTAGCTACTATTACGAGTCAACAGGAGAAAGTGAACTTAATCTAGAACTAATGAAACTAATTG
ACCAACAATATACTCAAACGCCTTTTTACGGCATTAGACGCATGACAGCTTGCCTTCAAAAGCAAGGTTATCAAGTTAATCATAAACGAGTAGCTCGTTT
GATGAGAAAAATGGGGTTAGAAACCATTTATCCGCGTCCAAAGCTAAGCCAGTCCGATGATAATCATCGTCGCTATCCTTATCTACTTAACGATCTGACC
ATTGATCAACCGAACCAAGTTTGGTGTAGCGACATTACCTATATCCGAATGCCCAAAGGATTCGTCTACTTGGTGGCCATCATGGATTGGTATTCTCGCT
TTGTGCTGGCATGGCAGTTATCCAATACTCTCGAAGTCAGTTTTTGTCTACAAGCGTTGGCGATCGCAGCTAAATGGGGTAAGCCTGAGATTTTTAATTC
AGATCAAGGCTCTCAATATACCAGTCGTGAATTTGTGGACTCCTTAGAAAATCTGGGAATTACCATTAGTCAAGATGGAAGAGGCCGAGTCTTTGATAAC
ATCTTTATTGAACGTTTATGGCGTTCTGTAAAGTACGAAGAAGTTTATCTCAAAGATTATTCTACTGTAGCGATCGCCTTAGAAAACCTGAGCAACTACT
TTGAGTTTTATAACTATCGTAGGTTGCATCAATCTTTGGGTTATCAATGTCCAGCTACAATACATTTTCAAGATTAAGGAGGTGATTTAATTAAATTAAC
AAAAGTCTAAAATCTAGTCAGGTCAAAAGCTAACAGCTTTCTATCTTAGTTTTATTAGTTATTTGTCTAGACAATTGGGTACA
Recoding section
- Recoding by frameshift
- Frame +1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 324 bp | 107 aa | 37 | 360 | + | No |
Description : First part of the transposase
ORF sequence :
MLLLNYQQQEKKMQRKRYSAEFKAKIALEAIKGLKTVNEIAGEFGVHPTQIQQWKKQLLERLPDIFSQKQEKRKKQDEELTAQLYQQIGQLKVELDWVKK
KAGLLGK
KAGLLGK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 846 bp | 281 aa | 332 | 1177 | + | No |
Description : Second part of the transposase
ORF sequence :
KKKLDCSVSDKRTLIEPEHSQINLSRQCELLGLARSSYYYESTGESELNLELMKLIDQQYTQTPFYGIRRMTACLQKQGYQVNHKRVARLMRKMGLETIY
PRPKLSQSDDNHRRYPYLLNDLTIDQPNQVWCSDITYIRMPKGFVYLVAIMDWYSRFVLAWQLSNTLEVSFCLQALAIAAKWGKPEIFNSDQGSQYTSRE
FVDSLENLGITISQDGRGRVFDNIFIERLWRSVKYEEVYLKDYSTVAIALENLSNYFEFYNYRRLHQSLGYQCPATIHFQD
PRPKLSQSDDNHRRYPYLLNDLTIDQPNQVWCSDITYIRMPKGFVYLVAIMDWYSRFVLAWQLSNTLEVSFCLQALAIAAKWGKPEIFNSDQGSQYTSRE
FVDSLENLGITISQDGRGRVFDNIFIERLWRSVKYEEVYLKDYSTVAIALENLSNYFEFYNYRRLHQSLGYQCPATIHFQD
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1141 bp | 379 aa | 37 | 1177 | + | Yes |
Chemistry : DDE
ORF sequence :
MLLLNYQQQEKKMQRKRYSAEFKAKIALEAIKGLKTVNEIAGEFGVHPTQIQQWKKQLLERLPDIFSQKQEKRKKQDEELTAQLYQQIGQLKVELDWVKK
KLDCSVSDKRTLIEPEHSQINLSRQCELLGLARSSYYYESTGESELNLELMKLIDQQYTQTPFYGIRRMTACLQKQGYQVNHKRVARLMRKMGLETIYPR
PKLSQSDDNHRRYPYLLNDLTIDQPNQVWCSDITYIRMPKGFVYLVAIMDWYSRFVLAWQLSNTLEVSFCLQALAIAAKWGKPEIFNSDQGSQYTSREFV
DSLENLGITISQDGRGRVFDNIFIERLWRSVKYEEVYLKDYSTVAIALENLSNYFEFYNYRRLHQSLGYQCPATIHFQD
KLDCSVSDKRTLIEPEHSQINLSRQCELLGLARSSYYYESTGESELNLELMKLIDQQYTQTPFYGIRRMTACLQKQGYQVNHKRVARLMRKMGLETIYPR
PKLSQSDDNHRRYPYLLNDLTIDQPNQVWCSDITYIRMPKGFVYLVAIMDWYSRFVLAWQLSNTLEVSFCLQALAIAAKWGKPEIFNSDQGSQYTSREFV
DSLENLGITISQDGRGRVFDNIFIERLWRSVKYEEVYLKDYSTVAIALENLSNYFEFYNYRRLHQSLGYQCPATIHFQD
Blast result :
Comments
ISCysp23 is 72% aa similar to ISAzs33.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
References
1] Mayya A. Petrova (2013) Direct submission to ISfinder
2] Welsh,E.A., Liberton,M., Stockel,J., Loh,T., Elvitigala,T., Wang,C., Wollam,A., Fulton,R.S., Clifton,S.W., Jacobs,J.M., Aurora,R., Ghosh,B.K., Sherman,L.A., Smith,R.D., Wilson,R.K. and Pakrasi,H.B. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (39), 15094-15099
2] Welsh,E.A., Liberton,M., Stockel,J., Loh,T., Elvitigala,T., Wang,C., Wollam,A., Fulton,R.S., Clifton,S.W., Jacobs,J.M., Aurora,R., Ghosh,B.K., Sherman,L.A., Smith,R.D., Wilson,R.K. and Pakrasi,H.B. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (39), 15094-15099