IS51
- Family IS3
- Group IS51
Isoform Synonym(s) ISPsy21
Accession number | Transposition | Origin | Host |
---|---|---|---|
M14365 | Y | Pseudomonas syringae | Pseudomonas syringae pv. savastanoi TK1006R Pseudomonas syringae pv. savastanoi TK2009-5 plasmid pIAA1 Pseudomonas syringae pv. savastanoi TK1050 |
DNA section
IS Length : 1311 bp
Ends
IR Length : 26
IRL : TGAACCGCCCCGGGTTTCTCGGAGACTCCAACCTTTGAGAGGATGGAGCG
IRR : TGAACCGCCCCGGGTTTCTCGGAGACCTTTTGTTTGAGTCATGCCACCTG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TGAGCCATCC | CTG | CCATGCGNNN | 3 |
CCATTATGCA | AAC | CCTTAGCAGC | 3 |
DNA sequence
TGAACCGCCCCGGGTTTCTCGGAGACTCCAACCTTTGAGAGGATGGAGCGATGAAAAAACTACCGAAGTATTCCCCAGAGGTCCGTGAGCGGGCCATCAG
GATGGTTTTCGAACACCTTCCTGAGTACGAGTCGCAGTGGGCCACCATCAGTGCCATTGCCCCCAAGATCGGCTGCACGCCCGAGACATTACGGCTGTGG
GTTCGCCAATCTGAGCGCAACAGCGGCCAGCGTGATGGGCTGAGCACAGTCGAACGTGAGCGAGTCAAGCTCTTGGAGCGTGAGAACCGTCAGTTGCGTC
AAGCTAACGAGATCTTGCGTAAGGCCTCAGCCTATTTTGCCCAGGCGGAGCTCGACCGCCCATTCAAACGATGAAGACGTTTATTGATGAAAACCGCCAG
TCTTACGGGGTCGAGCCGATTTGCAAGGTGTTGCCAATCGCCCCGTCGACGTACTACCGGTACGCTGCTCAGCAGGCTGACCCCAGCCAGCAGTCAGCGC
GTGCGCAGCGTGACCAGGAACTGGGTGAGCACATCCAGCGAGTCTGGGATGAGCACTTCCAGGTATATGGCGTGCGCAAGGTCTGGCGACAATTGCTGCG
TGACGGACTGCAAGTCGCCCGCTGTACGGTGCAACGTCTGATGGGAGAGCTGGGCCTGCAAGCGTGGTACGCGGCAAGCCCGTCAAGACGACGGTCAGCG
ACCAAGCCAGGCCGTGCCCGCAGGATCATGTCAACCGTCAGTTTGTGGCTGAGTGCCCGAACGCCCTATGGGTATCGGACTTTACCTACGTCAGTACCTG
GCAGGGTTTTGTTTACGTGGCCTTCATCATTGATGTCTTTGCCCGATTCATCGTCGGTTGGAAGGTGTCCAGTTCAGCGCGTACCGATTTTGTACTCGAT
GCACCTGGAGCAAGCCCTTTACGCCCGTCGCCCAGTCAAGCAGGGTGGACTCATTCACCACAGTGACCGTGGCGTGCAGTACGTGTCCATTCGCTATACC
GAGCGTCTTGTCGAAGCCGGTATTGAGCCATCGGTCGGGAGTGTCGGTGACTCTTACGACAATGCTTTGGCTGAGACCATCAATGGTCTTTACAAGGCTG
AAGTGATTCATCGACGCTCTTGGCCCACACGGGGGGCGGTGGAGCTGGAAACCCTGAAATGGGTGGACTGGTTCAATCACCGCCGCCTGCTGGAGCCAAT
CGGTCATATTCCACCGGTCGAAGCAGAAGCAATCTATTATCAGCAACTCACCGAGTCTGCCCAGGTGGCATGACTCAAACAAAAGGTCTCCGAGAAACCC
GGGGCGGTTCA
GATGGTTTTCGAACACCTTCCTGAGTACGAGTCGCAGTGGGCCACCATCAGTGCCATTGCCCCCAAGATCGGCTGCACGCCCGAGACATTACGGCTGTGG
GTTCGCCAATCTGAGCGCAACAGCGGCCAGCGTGATGGGCTGAGCACAGTCGAACGTGAGCGAGTCAAGCTCTTGGAGCGTGAGAACCGTCAGTTGCGTC
AAGCTAACGAGATCTTGCGTAAGGCCTCAGCCTATTTTGCCCAGGCGGAGCTCGACCGCCCATTCAAACGATGAAGACGTTTATTGATGAAAACCGCCAG
TCTTACGGGGTCGAGCCGATTTGCAAGGTGTTGCCAATCGCCCCGTCGACGTACTACCGGTACGCTGCTCAGCAGGCTGACCCCAGCCAGCAGTCAGCGC
GTGCGCAGCGTGACCAGGAACTGGGTGAGCACATCCAGCGAGTCTGGGATGAGCACTTCCAGGTATATGGCGTGCGCAAGGTCTGGCGACAATTGCTGCG
TGACGGACTGCAAGTCGCCCGCTGTACGGTGCAACGTCTGATGGGAGAGCTGGGCCTGCAAGCGTGGTACGCGGCAAGCCCGTCAAGACGACGGTCAGCG
ACCAAGCCAGGCCGTGCCCGCAGGATCATGTCAACCGTCAGTTTGTGGCTGAGTGCCCGAACGCCCTATGGGTATCGGACTTTACCTACGTCAGTACCTG
GCAGGGTTTTGTTTACGTGGCCTTCATCATTGATGTCTTTGCCCGATTCATCGTCGGTTGGAAGGTGTCCAGTTCAGCGCGTACCGATTTTGTACTCGAT
GCACCTGGAGCAAGCCCTTTACGCCCGTCGCCCAGTCAAGCAGGGTGGACTCATTCACCACAGTGACCGTGGCGTGCAGTACGTGTCCATTCGCTATACC
GAGCGTCTTGTCGAAGCCGGTATTGAGCCATCGGTCGGGAGTGTCGGTGACTCTTACGACAATGCTTTGGCTGAGACCATCAATGGTCTTTACAAGGCTG
AAGTGATTCATCGACGCTCTTGGCCCACACGGGGGGCGGTGGAGCTGGAAACCCTGAAATGGGTGGACTGGTTCAATCACCGCCGCCTGCTGGAGCCAAT
CGGTCATATTCCACCGGTCGAAGCAGAAGCAATCTATTATCAGCAACTCACCGAGTCTGCCCAGGTGGCATGACTCAAACAAAAGGTCTCCGAGAAACCC
GGGGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
324 bp | 107 aa | 51 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MKKLPKYSPEVRERAIRMVFEHLPEYESQWATISAIAPKIGCTPETLRLWVRQSERNSGQRDGLSTVERERVKLLERENRQLRQANEILRKASAYFAQAE
LDRPFKR
LDRPFKR
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
951 bp | 316 aa | 323 | 1273 | + | No |
Description : Second part of the transposase
ORF sequence :
GLSLFCPGGARPPIQTMKTFIDENRQSYGVEPICKVLPIAPSTYYRYAAQQADPSQQSARAQRDQELGEHIQRVWDEHFQVYGVRKVWRQLLRDGLQVAR
CTVQRLMGELGLQAVVRGKPVKTTVSDQARPCPQDHVNRQFVAECPNALWVSDFTYVSTWQGFVYVAFIIDVFARFIVGWKVSSSARTDFVLDALEQALY
ARRPVKQGGLIHHSDRGVQYVSIRYTERLVEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRSWPTRGAVELETLKWVDWFNHRRLLEPIGHIPPVE
AEAIYYQQLTESAQVA
CTVQRLMGELGLQAVVRGKPVKTTVSDQARPCPQDHVNRQFVAECPNALWVSDFTYVSTWQGFVYVAFIIDVFARFIVGWKVSSSARTDFVLDALEQALY
ARRPVKQGGLIHHSDRGVQYVSIRYTERLVEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRSWPTRGAVELETLKWVDWFNHRRLLEPIGHIPPVE
AEAIYYQQLTESAQVA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1223 bp | 407 aa | 51 | 1273 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKLPKYSPEVRERAIRMVFEHLPEYESQWATISAIAPKIGCTPETLRLWVRQSERNSGQRDGLSTVERERVKLLERENRQLRQANEILRKASAYFCPGG
ARPPIQTMKTFIDENRQSYGVEPICKVLPIAPSTYYRYAAQQADPSQQSARAQRDQELGEHIQRVWDEHFQVYGVRKVWRQLLRDGLQVARCTVQRLMGE
LGLQAVVRGKPVKTTVSDQARPCPQDHVNRQFVAECPNALWVSDFTYVSTWQGFVYVAFIIDVFARFIVGWKVSSSARTDFVLDALEQALYARRPVKQGG
LIHHSDRGVQYVSIRYTERLVEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRSWPTRGAVELETLKWVDWFNHRRLLEPIGHIPPVEAEAIYYQQL
TESAQVA
ARPPIQTMKTFIDENRQSYGVEPICKVLPIAPSTYYRYAAQQADPSQQSARAQRDQELGEHIQRVWDEHFQVYGVRKVWRQLLRDGLQVARCTVQRLMGE
LGLQAVVRGKPVKTTVSDQARPCPQDHVNRQFVAECPNALWVSDFTYVSTWQGFVYVAFIIDVFARFIVGWKVSSSARTDFVLDALEQALYARRPVKQGG
LIHHSDRGVQYVSIRYTERLVEAGIEPSVGSVGDSYDNALAETINGLYKAEVIHRRSWPTRGAVELETLKWVDWFNHRRLLEPIGHIPPVEAEAIYYQQL
TESAQVA
Blast result :
Comments
Pseudomonas syringae pv. savastanoi causes olive and oleander knot disease, due to the secretion of plant hormones (trans-zeatin riboside and indole-3-acetic acid) by the bacterium. IS51 is associated with the loss of indolacetic acid production (virulence factor), by insertion in the iaaM gene (tryptophan 2-monooxygenase) and a polar effect on the iaaH gene (indoleacetamide hydrolase). By comparison to IS868, specific DNA sequence changes have been proposed: 657-658: addition of a C, and 418: deletion of a G; all coordinates have been changed accordingly. IS51 (coordinates 1 to 528) is 67.7 % identical to the TC-region of Agrobacterium tumefaciens pTi15955 (coordinates 15616 to 15087) (De Meirsman et al., 1987).
References
1] Comai, L., and Kosuge, T. (1983) J. Bacteriol. 154, 1162-1167.
2] Yamada, T., Lee, P.-D., and Kosuge, T. (1986) Proc. Natl. Acad. Sci., USA 83, 8263- 8267.
3] De Meirsman, C., Desair, J., Vanderleyden, J., Van Gool, A.P., and Jen, G.C. (1987) Nucl. Acids Res. 15, 10591.
4] Paulus, F., Canaday, J., and Otten, L. (1991) Mol. Plant Microbe Interact. 4, 190-197.
5] Soby, S., Kirkpatrick, B., and Kosuge, T. (1994) Plasmid 31, 21-30.
2] Yamada, T., Lee, P.-D., and Kosuge, T. (1986) Proc. Natl. Acad. Sci., USA 83, 8263- 8267.
3] De Meirsman, C., Desair, J., Vanderleyden, J., Van Gool, A.P., and Jen, G.C. (1987) Nucl. Acids Res. 15, 10591.
4] Paulus, F., Canaday, J., and Otten, L. (1991) Mol. Plant Microbe Interact. 4, 190-197.
5] Soby, S., Kirkpatrick, B., and Kosuge, T. (1994) Plasmid 31, 21-30.