ISfinder Mirror Site

ISMta1

Family ISH6
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AGIY01000010	ND	Methanolinea tarda	Methanolinea tarda NOBI-1

DNA section

IS Length : 1588 bp

Ends

IR Length : 20/25

IRL : GATCGTGTCCAAAAATCGGCTAAGATAATCCCTGATGGGATGATCTCACT
IRR : GGTTGTGTCACAAGATCGGCTAAGAACTCACCAGCAACAAAAGATCTGAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATAACTGTGC	AAACTTTT	TGATTCGTGG	8

DNA sequence

GATCGTGTCCAAAAATCGGCTAAGATAATCCCTGATGGGATGATCTCACTGGTGACAAACCAAAAGGAGATCACCCCAGATGGATATCGTGCACGCAATT
GAAGTAAAGATATGCATTTCTGAAGGCGGCCAGCCGATACCGATCGAGAGTCTTGTAGCGTCGGTGAAGAACCTTCAAATCGAAGCCAGGCTACTTGAAC
GTATACTCGAGGCAATGAACGAATATCTCGTCAATTCCTATTGTGGCGAGAGATATTCAAGACGTACTCGCGAAGAGCGATATATTCGTGCAGGGACATC
GGAGAAGACGATTGTGACATCGGTGGGATCCATAACCCTGAAGCTGAATAAGGTCCGGGATATCCAGGAGAACCGTATCTGGAAACCCCTGACAGACATT
GTACGGTTTGCAGGAAAACAGGTGTATCAACCCGATATCTCCATGATCAGCATAGATTTTGTTCAGAAGCTCAGTTACCGCGATACTACGGAAGAGTTAG
GGATCGTACTTCCCGAAGTGCCCTCGCGGATGACCATACATAACCGTATAAAGCAAATGAGCGGATTGTTAGAGGTGAACGAGATCGAGATCCCCATGGT
GGTGGCAATGGCAGATGGAACCAAGACCCACTCACAGGAACCGGGTAAGAAACAGAATGATATCCATGTCGTTCTCGGTGTGAGCGATGTGAGGAAAGTG
CTGCTCGGTGTCACGGTGAATCAATCCTGGAAATTCCTGTCAGATCTTGTTGACAATGAGAAGACACTCGCCGAGAATGCAGTCATCGTCAGTGATGGAG
AACCTGAACTGAGGGCAGCATTTGCAGATGAAGATATTCAGTTCCAGACTGACCTGATCCATGGGTTCCGGTTATTAGGATATAAGTTATGGGAAGATGG
CAAATTGAGTCTGAAAGAGAGAAAGGGAGTAATTACTGAGCTGAAAAAGTTACTACTCTCGCTGAAAAATGTTGTTATTTTTCATAAATCTGATCATCAA
CGAGTCGGTGAGAAAATTAACCAGGTCGTTGAGAGTATGGAAAATCTGGCTAACCGATTGTTCGAGAAAGGGTGTTCTCGAGCTGCTGCTTTTCTCAATA
CCTATTCGAATACCCTTGTGACATTTGCCCGATTGTCCCTGAATGGTATTCACGTTCCCTGGAACTCAAATATGATCGAACGGTTGATGGGTGAGATATC
AAAACGAGTAAAACACAAATGGATGCGATGGACCACTCAGGGACTCGAAGCTATACTGAGGTTTATTCTCGTGCGCTATACCAGTCCCTGGTTGTATCGA
CGATTTCGTCATGAATACCTGGGACTCCATGGTCTTTCATCAATTGCAATTCAAATCTCGATTGATTCACGTGCTTCCTAGATCTTGGATCCCATTTTGA
GTAAGTTGGGCAGGATAGAGTGGGGACTTCGCCCCACACCCCGATTACGATAGACGCCGCCGCCCCCGACGGGGCACCCCCGCGGCGGTCCTAATGGTTC
TCTTTTCTGATGACCGAAATACTTGTTAGCTGAGATAACTCAGATCTTTTGTTGCTGGTGAGTTCTTAGCCGATCTTGTGACACAACC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1302 bp	433 aa	80	1381	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDIVHAIEVKICISEGGQPIPIESLVASVKNLQIEARLLERILEAMNEYLVNSYCGERYSRRTREERYIRAGTSEKTIVTSVGSITLKLNKVRDIQENRI
WKPLTDIVRFAGKQVYQPDISMISIDFVQKLSYRDTTEELGIVLPEVPSRMTIHNRIKQMSGLLEVNEIEIPMVVAMADGTKTHSQEPGKKQNDIHVVLG
VSDVRKVLLGVTVNQSWKFLSDLVDNEKTLAENAVIVSDGEPELRAAFADEDIQFQTDLIHGFRLLGYKLWEDGKLSLKERKGVITELKKLLLSLKNVVI
FHKSDHQRVGEKINQVVESMENLANRLFEKGCSRAAAFLNTYSNTLVTFARLSLNGIHVPWNSNMIERLMGEISKRVKHKWMRWTTQGLEAILRFILVRY
TSPWLYRRFRHEYLGLHGLSSIAIQISIDSRAS

Blast result :

Comments

ISMta1 is 57% aa similar to ISHma5.

References

1] ISfinder annotation (2013)
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Land,M.L., Hauser,L., Imachi,H., Sekiguchi,Y., Kamagata,Y., Cadillo-Quiroz,H., Zinder,S., Liu,W.T., Tamaki,H. and Woyke,T.J. (2011) Direct submission GenBank.