ISPsy31
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Pseudomonas syringae | Pseudomonas syringae pv. actinidiae |
DNA section
IS Length : 1667 bp
Ends
IR Length : 19/28
IRL : CCCCCGGTGTCAGGATAGTTGTCGCCTCTCCGGTTTTTCCTAAAAAAGCA
IRR : CGGCGTTTTTCAAGGTAGTTGTCGCGTCAACCGTCTAACTATGCTGCTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTGATCTTCT | CCAAG | TAACGATTCG | 5 |
| CGCGAGCGTG | AAGC | CGACATCACG | 4 |
DNA sequence
CCCCCGGTGTCAGGATAGTTGTCGCCTCTCCGGTTTTTCCTAAAAAAGCATGACCGGGGGCGGAAAGAGACTGATCGTGCCGTACTATTCGCCTGAACGC
AGAGCTGCATTACTCAAGATGCTGCTCCCCCCGCTGAACCTGTCGATGGCCGAGGTTTCCCGCCGCGAAGGGGTCAGTGAAATGTCTTTGTCCAACTGGC
GCAAGCAGCTCAGTTATGAAGGAAACGAAGTGTCTGAAAATACTCCCCTGACCGAAAAATGGTCAGCCGAAGCCAGGTTTGCTGTTGTGCTCGAAGCCGC
CGGTTTATCCGAGATCGACCTGAGCGAGTATTGTCGCCGCAAAGGCCTTTATCCCGAGCAGATCAACGCGTGGCGACACTCGTTCATCACGGGCCAGAAG
CCGGAAAAGACCCAGCAAAAAGAAGACCGTGAGCAATCTCGCAAAGACAAGAAACGCATACAGGAACTTGAGCGCGAACTGCGCCGCAAAGACAAGGCGC
TCGCTGAAACCGCGGCGTTGTTGGTGCTGCGAAAAAAGCTCAACGACTACTGGGGGACCGTCGACGAGGACAACTGACGTCCTTGCCTGAGCGCCTGTTA
CTGATTGACTGGCTAAATACCGCTGTTGCAGAGGGAGCCCGAAAAGCAAGGGCCTGCCAGGAAATCGACCTGTCATTGCGTACCTTGCAGCGATGGACGC
AGACAGACGTTTTCAAGGCTGATGCCCGCACAACGGTCACGCGGCCGACTCCGTCCAATGCACTGAGCGACGTTGAGCGCCAAGCTATTATCACACTGTG
CAACAGCCCTGAGTACGCTCACCTGCCGCCCAGCCAGATCGTGCCACGGCTGGCCGATCAAGGGTGTTATCTGGCGTCGGAGGCCACGTTTTATCGCGTC
TTGCGGGCCGCAGGTCAACAACAGCGTCGAGGGCGCAGTCAGCGTCCCAGACGATATGCGGCACCGACGACACATGCAGCCAAAGCGCCGAATCAGGTGT
GGTCGTGGGACATTACGTACCTGCCGTCACCGATAAGGGGCAAGTATTATTACCTCTATTTGATCGAGGATATTTACAGCCGCAAGGCCGTTGGCTGGGA
AGTTTACGAGATGGAAAGCGGCGAGAAAGCCGCCGCCCTGCTGCAACGCAGCGTGATAGGCGAGCAGTGTTTGCACGAGCCGCTGGTGCTGCACTCGGAC
AACGGTGCGCCGATGAAATCGGTGACGTTGTTAAGCAAGATGTATGAACTGGGCATCACCCCGTCGCGTGGGCGACCGCGTGTCAGCAACGACAATCCTT
ACTCGGAGTCGCTGTTCAGGACACTGAAATACTGCCCGCAATGGCCGCAGGACGGGTTTGCCAGCCTTGACGCGGCACGCCTCTGGGTGAGGGATTTCAT
GCGTTGGTACAACAATGATCATCGGCACAGCCGGATACGTTTCGTGACACCGACTGAGCGGCATCGAGGGCTGGACCATCAGATCCTGGCCAGGCGACAT
GAGCTGTACGAGCAAGCCAGAGAGAAAAGGCCAGAGCGGTGGTCGAGGGAGACACGCAACTGGGAACCGATCGGCACCGTGCTGTTAAACCCGGATCGAG
AGCAAAACGTTGAGAAAAAAGCAGCATAGTTAGACGGTTGACGCGACAACTACCTTGAAAAACGCCG
AGAGCTGCATTACTCAAGATGCTGCTCCCCCCGCTGAACCTGTCGATGGCCGAGGTTTCCCGCCGCGAAGGGGTCAGTGAAATGTCTTTGTCCAACTGGC
GCAAGCAGCTCAGTTATGAAGGAAACGAAGTGTCTGAAAATACTCCCCTGACCGAAAAATGGTCAGCCGAAGCCAGGTTTGCTGTTGTGCTCGAAGCCGC
CGGTTTATCCGAGATCGACCTGAGCGAGTATTGTCGCCGCAAAGGCCTTTATCCCGAGCAGATCAACGCGTGGCGACACTCGTTCATCACGGGCCAGAAG
CCGGAAAAGACCCAGCAAAAAGAAGACCGTGAGCAATCTCGCAAAGACAAGAAACGCATACAGGAACTTGAGCGCGAACTGCGCCGCAAAGACAAGGCGC
TCGCTGAAACCGCGGCGTTGTTGGTGCTGCGAAAAAAGCTCAACGACTACTGGGGGACCGTCGACGAGGACAACTGACGTCCTTGCCTGAGCGCCTGTTA
CTGATTGACTGGCTAAATACCGCTGTTGCAGAGGGAGCCCGAAAAGCAAGGGCCTGCCAGGAAATCGACCTGTCATTGCGTACCTTGCAGCGATGGACGC
AGACAGACGTTTTCAAGGCTGATGCCCGCACAACGGTCACGCGGCCGACTCCGTCCAATGCACTGAGCGACGTTGAGCGCCAAGCTATTATCACACTGTG
CAACAGCCCTGAGTACGCTCACCTGCCGCCCAGCCAGATCGTGCCACGGCTGGCCGATCAAGGGTGTTATCTGGCGTCGGAGGCCACGTTTTATCGCGTC
TTGCGGGCCGCAGGTCAACAACAGCGTCGAGGGCGCAGTCAGCGTCCCAGACGATATGCGGCACCGACGACACATGCAGCCAAAGCGCCGAATCAGGTGT
GGTCGTGGGACATTACGTACCTGCCGTCACCGATAAGGGGCAAGTATTATTACCTCTATTTGATCGAGGATATTTACAGCCGCAAGGCCGTTGGCTGGGA
AGTTTACGAGATGGAAAGCGGCGAGAAAGCCGCCGCCCTGCTGCAACGCAGCGTGATAGGCGAGCAGTGTTTGCACGAGCCGCTGGTGCTGCACTCGGAC
AACGGTGCGCCGATGAAATCGGTGACGTTGTTAAGCAAGATGTATGAACTGGGCATCACCCCGTCGCGTGGGCGACCGCGTGTCAGCAACGACAATCCTT
ACTCGGAGTCGCTGTTCAGGACACTGAAATACTGCCCGCAATGGCCGCAGGACGGGTTTGCCAGCCTTGACGCGGCACGCCTCTGGGTGAGGGATTTCAT
GCGTTGGTACAACAATGATCATCGGCACAGCCGGATACGTTTCGTGACACCGACTGAGCGGCATCGAGGGCTGGACCATCAGATCCTGGCCAGGCGACAT
GAGCTGTACGAGCAAGCCAGAGAGAAAAGGCCAGAGCGGTGGTCGAGGGAGACACGCAACTGGGAACCGATCGGCACCGTGCTGTTAAACCCGGATCGAG
AGCAAAACGTTGAGAAAAAAGCAGCATAGTTAGACGGTTGACGCGACAACTACCTTGAAAAACGCCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 528 bp | 175 aa | 50 | 577 | + | No |
Description : First part of the transposase
ORF sequence :
MTGGGKRLIVPYYSPERRAALLKMLLPPLNLSMAEVSRREGVSEMSLSNWRKQLSYEGNEVSENTPLTEKWSAEARFAVVLEAAGLSEIDLSEYCRRKGL
YPEQINAWRHSFITGQKPEKTQQKEDREQSRKDKKRIQELERELRRKDKALAETAALLVLRKKLNDYWGTVDEDN
YPEQINAWRHSFITGQKPEKTQQKEDREQSRKDKKRIQELERELRRKDKALAETAALLVLRKKLNDYWGTVDEDN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1122 bp | 373 aa | 508 | 1629 | + | No |
Description : Second part of the transposase
ORF sequence :
NRGVVGAAKKAQRLLGDRRRGQLTSLPERLLLIDWLNTAVAEGARKARACQEIDLSLRTLQRWTQTDVFKADARTTVTRPTPSNALSDVERQAIITLCNS
PEYAHLPPSQIVPRLADQGCYLASEATFYRVLRAAGQQQRRGRSQRPRRYAAPTTHAAKAPNQVWSWDITYLPSPIRGKYYYLYLIEDIYSRKAVGWEVY
EMESGEKAAALLQRSVIGEQCLHEPLVLHSDNGAPMKSVTLLSKMYELGITPSRGRPRVSNDNPYSESLFRTLKYCPQWPQDGFASLDAARLWVRDFMRW
YNNDHRHSRIRFVTPTERHRGLDHQILARRHELYEQAREKRPERWSRETRNWEPIGTVLLNPDREQNVEKKAA
PEYAHLPPSQIVPRLADQGCYLASEATFYRVLRAAGQQQRRGRSQRPRRYAAPTTHAAKAPNQVWSWDITYLPSPIRGKYYYLYLIEDIYSRKAVGWEVY
EMESGEKAAALLQRSVIGEQCLHEPLVLHSDNGAPMKSVTLLSKMYELGITPSRGRPRVSNDNPYSESLFRTLKYCPQWPQDGFASLDAARLWVRDFMRW
YNNDHRHSRIRFVTPTERHRGLDHQILARRHELYEQAREKRPERWSRETRNWEPIGTVLLNPDREQNVEKKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1580 bp | 526 aa | 50 | 1629 | + | Yes |
Chemistry : DDE
ORF sequence :
MTGGGKRLIVPYYSPERRAALLKMLLPPLNLSMAEVSRREGVSEMSLSNWRKQLSYEGNEVSENTPLTEKWSAEARFAVVLEAAGLSEIDLSEYCRRKGL
YPEQINAWRHSFITGQKPEKTQQKEDREQSRKDKKRIQELERELRRKDKALAETAALLVLRKKAQRLLGDRRRGQLTSLPERLLLIDWLNTAVAEGARKA
RACQEIDLSLRTLQRWTQTDVFKADARTTVTRPTPSNALSDVERQAIITLCNSPEYAHLPPSQIVPRLADQGCYLASEATFYRVLRAAGQQQRRGRSQRP
RRYAAPTTHAAKAPNQVWSWDITYLPSPIRGKYYYLYLIEDIYSRKAVGWEVYEMESGEKAAALLQRSVIGEQCLHEPLVLHSDNGAPMKSVTLLSKMYE
LGITPSRGRPRVSNDNPYSESLFRTLKYCPQWPQDGFASLDAARLWVRDFMRWYNNDHRHSRIRFVTPTERHRGLDHQILARRHELYEQAREKRPERWSR
ETRNWEPIGTVLLNPDREQNVEKKAA
YPEQINAWRHSFITGQKPEKTQQKEDREQSRKDKKRIQELERELRRKDKALAETAALLVLRKKAQRLLGDRRRGQLTSLPERLLLIDWLNTAVAEGARKA
RACQEIDLSLRTLQRWTQTDVFKADARTTVTRPTPSNALSDVERQAIITLCNSPEYAHLPPSQIVPRLADQGCYLASEATFYRVLRAAGQQQRRGRSQRP
RRYAAPTTHAAKAPNQVWSWDITYLPSPIRGKYYYLYLIEDIYSRKAVGWEVYEMESGEKAAALLQRSVIGEQCLHEPLVLHSDNGAPMKSVTLLSKMYE
LGITPSRGRPRVSNDNPYSESLFRTLKYCPQWPQDGFASLDAARLWVRDFMRWYNNDHRHSRIRFVTPTERHRGLDHQILARRHELYEQAREKRPERWSR
ETRNWEPIGTVLLNPDREQNVEKKAA
Blast result :
Comments
ISPsy31 is 92% aa similar to IS1240.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Margi Butler (2012) Direct submission