ISHvo16
- Family IS630
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP001955 | ND | Haloferax volcanii | Haloferax volcanii DS2 plasmid pHV4 |
DNA section
IS Length : 1055 bp
Ends
IR Length : 22/26
IRL : TAGTAATCGTTAGAACTTTTTACTCAGAGACTCTTGTAAGAGATAGTGAA
IRR : TAGTATTCGTTAGAAGTAATTACTCACTTTTGGGACCGAAAGCTGGTCAA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TAATCCACCTAC | TA | GCATGTTTGGTG | 2 |
ACGACTGCGAGT | TA | GGTCCCGACGCG | 2 |
DNA sequence
TAGTAATCGTTAGAACTTTTTACTCAGAGACTCTTGTAAGAGATAGTGAACCATCTCGACGAGATTTCCGTCGAGGAATTACAAGACACTCTCGACAACG
TGGACGGAAAGAAGCCGACACAACGGCTCTTAGCGGCTATCGCGTACAAGAACGGTGTCACGCAGACCGAGCTAGCCGAGTGGTACGGTGTTCAACGCCG
GACGATCTACAGCTGGCTCAAGCGACTCGACACCGACGAGTCGCTTGAGCAAGCCGTTTCTGACGATAAACGAACCGGAAGGAAGCGAAAGCTTTCAGAA
ACACAGCAAAGCGAATTTCAAGAGACCGTCCACGAACCTCCTGAAGAAGTTGGGCTCGACGCGCCGGCGTGGACGCCGGCGCTTGCCCAAGAATTTCTCG
AGGAAACATACGGCGTCGAGTATTCAATCCCGAGTTGTCGGCGGTTGCTGAAAGAAGCGGGATTGAGATATCAGAAACCACGCCGTACAGCCGCCGAAGC
CGACGAAGACGAGCAAGAAGCGTTCTACGAAGAACTCAAAAAAAGCGGCGGAAGATGGACGCCACCGTAGTCTGTATCGACCAAACCAAGAAATCCGTGC
AAGTTGAGCCGCGTGCCGCGTGGTTTCCACGTGGCACGCGGCCCTCGGTCAAACTCTCTGGTCAACGCGACTGGACGTGTCTGCTGGGCGCGATCACCGA
GGACGGTGATCGCTTCTTTTCACGATTCGAGGAGTACGTCACCGCTGAACACGCGAAACATTTCATTTTAGCACTATGTAAAGAATTTGAAGATGACTTG
CTCGTGGTGCTAGATGGTGCGCCGTACTTTCAAGCGTCGGCCGTCACGGACCTGGCGGCCCGTGACGACCTCGCGTTCGTGACGCTTCCATCATACTCGC
CGGAACTAAACCCGGTTGAGGAGTGCTGGAGACAGCTTCAAGCAGCGTTGAGCAACCGGTTTTTCGACTCACTTGACGAGTTAACGACAGCGATAGACGC
CGCTCTTGACCAGCTTTCGGTCCCAAAAGTGAGTAATTACTTCTAACGAATACTA
TGGACGGAAAGAAGCCGACACAACGGCTCTTAGCGGCTATCGCGTACAAGAACGGTGTCACGCAGACCGAGCTAGCCGAGTGGTACGGTGTTCAACGCCG
GACGATCTACAGCTGGCTCAAGCGACTCGACACCGACGAGTCGCTTGAGCAAGCCGTTTCTGACGATAAACGAACCGGAAGGAAGCGAAAGCTTTCAGAA
ACACAGCAAAGCGAATTTCAAGAGACCGTCCACGAACCTCCTGAAGAAGTTGGGCTCGACGCGCCGGCGTGGACGCCGGCGCTTGCCCAAGAATTTCTCG
AGGAAACATACGGCGTCGAGTATTCAATCCCGAGTTGTCGGCGGTTGCTGAAAGAAGCGGGATTGAGATATCAGAAACCACGCCGTACAGCCGCCGAAGC
CGACGAAGACGAGCAAGAAGCGTTCTACGAAGAACTCAAAAAAAGCGGCGGAAGATGGACGCCACCGTAGTCTGTATCGACCAAACCAAGAAATCCGTGC
AAGTTGAGCCGCGTGCCGCGTGGTTTCCACGTGGCACGCGGCCCTCGGTCAAACTCTCTGGTCAACGCGACTGGACGTGTCTGCTGGGCGCGATCACCGA
GGACGGTGATCGCTTCTTTTCACGATTCGAGGAGTACGTCACCGCTGAACACGCGAAACATTTCATTTTAGCACTATGTAAAGAATTTGAAGATGACTTG
CTCGTGGTGCTAGATGGTGCGCCGTACTTTCAAGCGTCGGCCGTCACGGACCTGGCGGCCCGTGACGACCTCGCGTTCGTGACGCTTCCATCATACTCGC
CGGAACTAAACCCGGTTGAGGAGTGCTGGAGACAGCTTCAAGCAGCGTTGAGCAACCGGTTTTTCGACTCACTTGACGAGTTAACGACAGCGATAGACGC
CGCTCTTGACCAGCTTTCGGTCCCAAAAGTGAGTAATTACTTCTAACGAATACTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
525 bp | 174 aa | 46 | 570 | + | No |
Description : First part of the transposase
ORF sequence :
MNHLDEISVEELQDTLDNVDGKKPTQRLLAAIAYKNGVTQTELAEWYGVQRRTIYSWLKRLDTDESLEQAVSDDKRTGRKRKLSETQQSEFQETVHEPPE
EVGLDAPAWTPALAQEFLEETYGVEYSIPSCRRLLKEAGLRYQKPRRTAAEADEDEQEAFYEELKKSGGRWTPP
EVGLDAPAWTPALAQEFLEETYGVEYSIPSCRRLLKEAGLRYQKPRRTAAEADEDEQEAFYEELKKSGGRWTPP
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
702 bp | 233 aa | 345 | 1046 | + | No |
Description : Second part of the transposase
ORF sequence :
RSWARRAGVDAGACPRISRGNIRRRVFNPELSAVAERSGIEISETTPYSRRSRRRRARSVLRRTQKKRRKMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVKLSGQRDWTCLLGAITEDGDRFFSRFEEYVTAEHAKHFILALCKEFEDDLLVVLDGAPYFQASAVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQA
ALSNRFFDSLDELTTAIDAALDQLSVPKVSNYF
SVKLSGQRDWTCLLGAITEDGDRFFSRFEEYVTAEHAKHFILALCKEFEDDLLVVLDGAPYFQASAVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQA
ALSNRFFDSLDELTTAIDAALDQLSVPKVSNYF
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1001 bp | 333 aa | 46 | 1046 | + | Yes |
Chemistry : DDE
ORF sequence :
MNHLDEISVEELQDTLDNVDGKKPTQRLLAAIAYKNGVTQTELAEWYGVQRRTIYSWLKRLDTDESLEQAVSDDKRTGRKRKLSETQQSEFQETVHEPPE
EVGLDAPAWTPALAQEFLEETYGVEYSIPSCRRLLKEAGLRYQKPRRTAAEADEDEQEAFYEELKKKRRKMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVKLSGQRDWTCLLGAITEDGDRFFSRFEEYVTAEHAKHFILALCKEFEDDLLVVLDGAPYFQASAVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQA
ALSNRFFDSLDELTTAIDAALDQLSVPKVSNYF
EVGLDAPAWTPALAQEFLEETYGVEYSIPSCRRLLKEAGLRYQKPRRTAAEADEDEQEAFYEELKKKRRKMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVKLSGQRDWTCLLGAITEDGDRFFSRFEEYVTAEHAKHFILALCKEFEDDLLVVLDGAPYFQASAVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQA
ALSNRFFDSLDELTTAIDAALDQLSVPKVSNYF
Blast result :
Comments
ISHvo16 is 57% aa similar to ISHwa16. IS_pep_3 is HVO_A0100.
The third ORF is an ORFAB transposase reconstructed in silico by a +1 frameshift probably reflecting ribosomal slippage.
The third ORF is an ORFAB transposase reconstructed in silico by a +1 frameshift probably reflecting ribosomal slippage.
References
1] Pfeiffer, F. (2013) Direct submission
2] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605
2] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605