ISHvo12
- Family IS4
- Group ISH8
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001956 | ND | Haloferax volcanii | Haloferax volcanii DS2 plasmid pHV4 Haloferax volcanii DS2 plasmid pHV1 Haloferax volcanii DS2 |
DNA section
IS Length : 1395 bp
Ends
IR Length : 17/19
IRL : CATCCGTGTTCGGTTAAGCGGAGAAACCGACAGACGGCGCTCTAGTGACG
IRR : CATTGGTGTTCGGTTAAGCACTGGATCGCCTCCTGAAGGCCTCAGCTACT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCAACTGTTTG | CTCTTCAGAG | GAAAGATTCTCA | 10 |
| AGCTTGTGCTGC | CTCTCCAGAG | GCATCGTCGTAA | 10 |
| GGGGGGTGTATC | GTCCTAAGAC | GTACGGTGATAT | 10 |
| TGGCAAAGCGAT | TTGCTTGGACAC | 0 | |
| AGGGATCTCGAC | CCAGATCCACAC | 0 |
DNA sequence
CATCCGTGTTCGGTTAAGCGGAGAAACCGACAGACGGCGCTCTAGTGACGAAGCTATCGTTGTAGACCGCGTGGGGAGAGAATGTGCTAAACCAACTCTC
CCCGGATCTCATACGACGACGGTTGACTTCCCTGTTTCCCACCGAGCTAATCGAAGACATCGCGCGCGAGCGCGATGTCGTCCAGCGTGACCGGAAAATC
GACATCACGATGCTCGTCTGGACGCTCATCATGGGCTTCGCCGTCGACGGCGAAGCCCGAACTATCGCCGGCTTTCAACGGGCGTACTCCGCAGCAACCA
ACCAGACCGTTGCCCGCTCAAGCTTCTACGATCGGTTCACGCCGGCCCTCGCAGCACTGTTGAACGACCTCCTCGAGCACGCTCTCGAGGAGGTCGCGGT
TCCTCACACGATCGCGCCACAGTTCGAGCTGTTTCGAGACGTACTGATCGCCGATGCAACCGTGTTCAGGTTGCATCGGCTCCTCGATTCGTTTCCAGCC
ACTCACTCGGGTCAATCCGGCGCGAAACTCCACCTCGTGCAGAACGCGACCAAGCAGACGATCGAGCAGTTCCAGCTCACCGATGAACGCACCCACGAGA
GCAGCCAACTCCGCACCGGGAGTTGGCTACGAGGCCGGCTGTTACTGTTCGATCTGGGCTTCTACAGTTTCCGCCGCTTCGCGTTGATCGAGGAGAACGG
CGGGTTCTTCCTGAGTCGGCTGAAGTCGAACGCGAATCCGTTGATCGTTGGAGAGCGACGGAAATGGCGCGGGCGCGCCATTTCCTTGCCAGAGCGTCGT
CTTCGGGACGTTCTGGAAGATCTCAGTCGGGAGATCATCGACGTAACGGTAGAGATCGAGTTCAAACGGCGGGCATACGCTGGGAAGGAATCAACGGATT
CGATGGAGTTTCGCGTCGTCGGTGTCCGCAACGAGGACACCGACGACTACCACCTGTACGTGACGAATCTGCCCGATGAGTTCACTCCAAGGCAGGTCGG
GGCGCTGTACGGGTTGCGGTGGGAAGTGGAGTTGGTGTTCCGGGAACTGAAGTCGCTGTATGGGCTGGAGAAATTCCAGACGAGTGATCCAGCGATCGTG
GAGCTGTTAGTGGTGGCGGCTCTGCTGACGCTGACGGTCAGCAGAGCCTTGCTCGGGGTGTTTCAGGAGCTGTTCCCAGAGACAGTGTTTCCGCGAGAAC
GCTGGGCGAAGACCTTCCGGTCTCTCGCCCAGCTCATGCTCGAAGATCTGGCCCAGTCGTTCGGCCATCCACCGCCGAACCTGTCGGAACTGCTGTTTCG
TGACGCCCGCCAACCAGAGAAATCGCGGCTCTTACTCAACGAACGAGTAGCTGAGGCCTTCAGGAGGCGATCCAGTGCTTAACCGAACACCAATG
CCCGGATCTCATACGACGACGGTTGACTTCCCTGTTTCCCACCGAGCTAATCGAAGACATCGCGCGCGAGCGCGATGTCGTCCAGCGTGACCGGAAAATC
GACATCACGATGCTCGTCTGGACGCTCATCATGGGCTTCGCCGTCGACGGCGAAGCCCGAACTATCGCCGGCTTTCAACGGGCGTACTCCGCAGCAACCA
ACCAGACCGTTGCCCGCTCAAGCTTCTACGATCGGTTCACGCCGGCCCTCGCAGCACTGTTGAACGACCTCCTCGAGCACGCTCTCGAGGAGGTCGCGGT
TCCTCACACGATCGCGCCACAGTTCGAGCTGTTTCGAGACGTACTGATCGCCGATGCAACCGTGTTCAGGTTGCATCGGCTCCTCGATTCGTTTCCAGCC
ACTCACTCGGGTCAATCCGGCGCGAAACTCCACCTCGTGCAGAACGCGACCAAGCAGACGATCGAGCAGTTCCAGCTCACCGATGAACGCACCCACGAGA
GCAGCCAACTCCGCACCGGGAGTTGGCTACGAGGCCGGCTGTTACTGTTCGATCTGGGCTTCTACAGTTTCCGCCGCTTCGCGTTGATCGAGGAGAACGG
CGGGTTCTTCCTGAGTCGGCTGAAGTCGAACGCGAATCCGTTGATCGTTGGAGAGCGACGGAAATGGCGCGGGCGCGCCATTTCCTTGCCAGAGCGTCGT
CTTCGGGACGTTCTGGAAGATCTCAGTCGGGAGATCATCGACGTAACGGTAGAGATCGAGTTCAAACGGCGGGCATACGCTGGGAAGGAATCAACGGATT
CGATGGAGTTTCGCGTCGTCGGTGTCCGCAACGAGGACACCGACGACTACCACCTGTACGTGACGAATCTGCCCGATGAGTTCACTCCAAGGCAGGTCGG
GGCGCTGTACGGGTTGCGGTGGGAAGTGGAGTTGGTGTTCCGGGAACTGAAGTCGCTGTATGGGCTGGAGAAATTCCAGACGAGTGATCCAGCGATCGTG
GAGCTGTTAGTGGTGGCGGCTCTGCTGACGCTGACGGTCAGCAGAGCCTTGCTCGGGGTGTTTCAGGAGCTGTTCCCAGAGACAGTGTTTCCGCGAGAAC
GCTGGGCGAAGACCTTCCGGTCTCTCGCCCAGCTCATGCTCGAAGATCTGGCCCAGTCGTTCGGCCATCCACCGCCGAACCTGTCGGAACTGCTGTTTCG
TGACGCCCGCCAACCAGAGAAATCGCGGCTCTTACTCAACGAACGAGTAGCTGAGGCCTTCAGGAGGCGATCCAGTGCTTAACCGAACACCAATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1299 bp | 432 aa | 84 | 1382 | + | No |
Chemistry : DDE
ORF sequence :
MLNQLSPDLIRRRLTSLFPTELIEDIARERDVVQRDRKIDITMLVWTLIMGFAVDGEARTIAGFQRAYSAATNQTVARSSFYDRFTPALAALLNDLLEHA
LEEVAVPHTIAPQFELFRDVLIADATVFRLHRLLDSFPATHSGQSGAKLHLVQNATKQTIEQFQLTDERTHESSQLRTGSWLRGRLLLFDLGFYSFRRFA
LIEENGGFFLSRLKSNANPLIVGERRKWRGRAISLPERRLRDVLEDLSREIIDVTVEIEFKRRAYAGKESTDSMEFRVVGVRNEDTDDYHLYVTNLPDEF
TPRQVGALYGLRWEVELVFRELKSLYGLEKFQTSDPAIVELLVVAALLTLTVSRALLGVFQELFPETVFPRERWAKTFRSLAQLMLEDLAQSFGHPPPNL
SELLFRDARQPEKSRLLLNERVAEAFRRRSSA
LEEVAVPHTIAPQFELFRDVLIADATVFRLHRLLDSFPATHSGQSGAKLHLVQNATKQTIEQFQLTDERTHESSQLRTGSWLRGRLLLFDLGFYSFRRFA
LIEENGGFFLSRLKSNANPLIVGERRKWRGRAISLPERRLRDVLEDLSREIIDVTVEIEFKRRAYAGKESTDSMEFRVVGVRNEDTDDYHLYVTNLPDEF
TPRQVGALYGLRWEVELVFRELKSLYGLEKFQTSDPAIVELLVVAALLTLTVSRALLGVFQELFPETVFPRERWAKTFRSLAQLMLEDLAQSFGHPPPNL
SELLFRDARQPEKSRLLLNERVAEAFRRRSSA
Blast result :
Comments
ISHvo12 is 71% aa similar to ISHma1.
IS_pep_1 is HVO_1106, HVO_2830, HVO_A0080, HVO_A0456, and HVO_C0013.
IS_pep_1 is HVO_1106, HVO_2830, HVO_A0080, HVO_A0456, and HVO_C0013.
References
1] Pfeiffer, F. (2013) Direct submission
2] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605
2] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605