ISKpn18

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP003200 ND Klebsiella pneumoniae
Klebsiella pneumoniae subsp. pneumoniae HS11286 plasmid pKPHS3
Klebsiella pneumoniae subsp. pneumoniae HS11286
DNA section
IS Length : 1303 bp

Ends


IR Length : 26/33

IRL : TGTTGTGGTCAAATTTAAATGGACACTTTGATCGGGTGTTTTCCCCCCGA
IRR : TGTTGTGGTCAAGTTTTTTTGGACACGCCGATCAGATGGTAGGGTTATGC

Insertion site


Left flankDirect repeatRight flankDR Length
TGCCACATGGTGGCTTCAAAGGC3
GCTTAACGCTGACAAACAGCCGC3
TCATCGACACCTCTGTAACC0

DNA sequence

TGTTGTGGTCAAATTTAAATGGACACTTTGATCGGGTGTTTTCCCCCCGACAAGGAACATGAAGAGATGACCGACAAGACAACCGACACAACCAAGAATC
GCCGAACCTTTGATCCAGCGTTCAAGTTACAGGTGGCGCGCATGGTGCGCGAGCAAGGCGTCAGCGTCAGCCAAGTCTGCCGCGAAATGAAGCTGACCGA
AAGTGTGGTCCGACGCTGGGTACACCAACTGGATGACGAGTCAGCCGGCAGGCCGGGGATTGGTAAACCGTTAACGGCTGAGCAGCAACGTATTCGAGAA
CTGGAAGCCGAAGTCAGGCGACTAAAATCAGACAATGAATTATTAAAAAAAGCTTCGGCCTTCTTCGCACGGGAAATAAAGTGATCCACGATGTTGTCAC
TGAGTTAGCGAAGCAGGCCCCGATCAAGCAGGTCTGTCAGGCGTTGGACATCAGTCGCTCTGGTTACTACGCAGCGCGTCGCAGAGTCAATACACCCAAA
CCTATTTGCCAGGACAGCGTGCATGCTCAGGCGGTTTTTCTTGCCAGTGGTCGCACCTATGGCAGTCGTCGTTTGAGTGCAGCCCTGCGTGCCCAGGGCC
ATGATGTTGGACGTCATCGCTGTCGTACCTTGATGAAAGCCTGTGGACTCAAGGCCTGTTGGCGTCGCAAGTTCGCTCATACCACCGATAGCAAACACCA
TTTGCCGGTGGCAGAGAACGTGCTCAACCGTCAGTTTGAGCCTGAAGCACCCAATACGGCCTGGGTGTGCGATGTGACGTACATTCGGACCGACAGCGGT
TGGCTATATCTGGCCGCTGTGCTGGATCTGTATTCACGTCGCATCGTGGGTTGGTCCATGGCACCGACCATGCCTGCACAGCTGGTGTGCGATGCGTTGA
GCATGGCTATTTCGGTTCGCCAACCGGCACCGGGTTTATTGGTTCATTCGGACCGGGGCAGTCAATATGCCAGTGCTGAACACCGGCAATTACTTGAAAA
CCATGGTCTGGTACTCAGCATGAGCCGCAAGGGTAATTGCTGGGACAATGCCGTGATGGAGCGGTTCTTTCTGAATTTGAAAATGGAGCGTGTCTGGCAG
ACTCGGTATGCCAACCATACAGAAGCCAAACAAGACATCACCGACTACATCGTTAACTTTTATAACAGTACCCGATTGCATTCAAAACTTGGCTACCGAT
CACCCGCACAGTACGAAACGCTGGCCAGCCCTGCCGCCCAGTTGCCCGCCTGTGCATAACCCTACCATCTGATCGGCGTGTCCAAAAAAACTTGACCACA
ACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
318 bp105 aa67384+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTDKTTDTTKNRRTFDPAFKLQVARMVREQGVSVSQVCREMKLTESVVRRWVHQLDDESAGRPGIGKPLTAEQQRIRELEAEVRRLKSDNELLKKASAFF
AREIK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
921 bp306 aa3391259+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IIKKSFGLLRTGNKVIHDVVTELAKQAPIKQVCQALDISRSGYYAARRRVNTPKPICQDSVHAQAVFLASGRTYGSRRLSAALRAQGHDVGRHRCRTLMK
ACGLKACWRRKFAHTTDSKHHLPVAENVLNRQFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCDALSMAISVRQPAPGL
LVHSDRGSQYASAEHRQLLENHGLVLSMSRKGNCWDNAVMERFFLNLKMERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAA
QLPACA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1193 bp397 aa671259+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTDKTTDTTKNRRTFDPAFKLQVARMVREQGVSVSQVCREMKLTESVVRRWVHQLDDESAGRPGIGKPLTAEQQRIRELEAEVRRLKSDNELLKKSFGLL
RTGNKVIHDVVTELAKQAPIKQVCQALDISRSGYYAARRRVNTPKPICQDSVHAQAVFLASGRTYGSRRLSAALRAQGHDVGRHRCRTLMKACGLKACWR
RKFAHTTDSKHHLPVAENVLNRQFEPEAPNTAWVCDVTYIRTDSGWLYLAAVLDLYSRRIVGWSMAPTMPAQLVCDALSMAISVRQPAPGLLVHSDRGSQ
YASAEHRQLLENHGLVLSMSRKGNCWDNAVMERFFLNLKMERVWQTRYANHTEAKQDITDYIVNFYNSTRLHSKLGYRSPAQYETLASPAAQLPACA

 

Blast result :
Comments
ISKpn18 is 63% aa similar to ISPosp5.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] A. Garcia-Fernandez (2013) Direct submission