ISHal1

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AOLL01000028 ND Haloferax alexandrinus
Haloferax volcanii DS2
Haloferax alexandrinus JCM 10717
DNA section
IS Length : 1846 bp

Ends


Left end : TTTCAAGGCGAGAATCCCGCCGTTTACGGCGGGCGTGAAGCCGACAACTACCTCACGTTCTACCGTCGGTTGCAGGCCGAATACCCCACGACTACGAGCC II struct. : Yes
Right end : GCTCACGGCCTACGAAGAGTCGAAACCCTCTGCCAGCGACTACTGACTGGTATTCCCCATGCGTGGGAAGCCTCGCCGTTTACGGCGAGGAGGATGTCAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GACCTGCTATCTTTCGACGATTACTCAC
CACTCGCTTCCTTTCAGACGGGTCTCAc

DNA sequence

TTTCAAGGCGAGAATCCCGCCGTTTACGGCGGGCGTGAAGCCGACAACTACCTCACGTTCTACCGTCGGTTGCAGGCCGAATACCCCACGACTACGAGCC
TTTATTAGAATTGGGTGTGTAGAAACTAGTACGGCCAAATGAAGTACAACCTTGAAACCGGGTCGCACACGGTCTACGCGCTCCAATATCACTTCGTGAC
CGTCACGAAGTACCGCGCAGACCTCCTCACCGACGAAATCGCAGAGCGCATCGGTGAGATTGCCAGCGACATCTCCGAGGACTTCGGCGTGAACATCCAG
AACGTCAACGGCGGAAGCGACCACGTTCACATCCTCTTCACGGCGAAGCCAACGACCGACCTCACCAAGTTCATCAACTCACTCAAGGGCGTTACGTCCC
GCAAAATCCGTGACGAGAACCCCGAGGTTCGGCAGGCGCCCGACAAAGCGTTCTGGCAACCGGGATACTTCCTCGCCACCACCGGCCAAGTGAGTATCGA
CGTACTCATGAAGTACGTGGAGGAGCAGTAGCGTGTCTCCGACCGTCACGAAGACGTTGCAGGCGACGTTCGCGCCACCCACCGCGCACAAGCAGTCGAA
ACTCAACGACCTGCTGGAAACCTACCGTGACGGTCTGCAAGAGGCGTTCGACGCCGGGGCGAGTACCATGTCGGCGGTGAGTGAAATCGTGACGCCCTAC
GACCTGCCGTATCAGGCCAAAGCCGCGCTCTGCAACTACGTCCCGAAACTCCGCAAGACGTACAGCGCGAAGGAGTTGGACGACAGCCACCCGATACGGC
TCACGAACCAAGCCGCGAAGTTCGACCACTCCGCCGAACGCGACTACGAGTTCACGTGGTGGGTTCCCCGTCCCGGTCGGGGAACGAACTTCTGGATACC
GCTCCGTATCAACCCCGAACAGGAAGGCCTCTGGCACGACCTCGTATCGGAGGACGCGAAGGCGGGTGAGATACGGCTTCAGAAGCATCGGAAGAACTGG
GTACTGCACGTCACCGTCGAGTACCCGGTCGAAGAACCAGCGACGGACGGTGACGCCACGCACATCGGCTTAGACATCGGAGAAACCGCTCTCATCACGG
GCTGTGCCCTCAAGGATGGTTCTCCGACTGATCCGTTCGTGTGTAGCGGAAGCAGAGCGAAGCATCTCCGCAAAGAGATGCACACGACCCTGAAACGACT
CCAAGAGCGTGACGCATCCGAGTGGCGGATTGAAGACCGATTCAGCCACTACCAGAACGCGCTCACCGACATCGTGGAGAAAGTGTCTCGGCAAGCCGTC
GAGTACGCCCAACAGTTCGAGAACCCGGTGTTGGTGATGGAGGACTTAACGTACATCCGTGAACGGCTTGACTACGGGAAGTACATGAACCGTCGCCTTC
ACTCGTGGGCGTTCGCCCGACTCCAAGGGCGCATCGAGGACAAGGCGACGGAAGCAGGCATTCCGGTCGAGTACGTGAATCCGGCGTACACCTCGCAGAC
GTGCCACTCGTGCCACCGTATCGGTCGGCGGGACTCACAAGCCGAGTTCCGGTGTCCGAACGACGACTGCCACGTTTCGACGTTTCAGGCCGACATCAAC
GCTTCCGCTAATATCGCACGACGGGTTGACCCGTGGGGAGAGAGCGTCCCGCTTGACAAGGCGGAACGCGATGACTCGCCACGGGATGGGAGCAGTTGTG
ACACCGCCACGACTCACCGTGAGACGAGCGTACCAGCGCAGATGACGCTCACGGCCTACGAAGAGTCGAAACCCTCTGCCAGCGACTACTGACTGGTATT
CCCCATGCGTGGGAAGCCTCGCCGTTTACGGCGAGGAGGATGTCAC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
393 bp130 aa139531+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKYNLETGSHTVYALQYHFVTVTKYRADLLTDEIAERIGEIASDISEDFGVNIQNVNGGSDHVHILFTAKPTTDLTKFINSLKGVTSRKIRDENPEVRQA
PDKAFWQPGYFLATTGQVSIDVLMKYVEEQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1260 bp419 aa5331792+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MSPTVTKTLQATFAPPTAHKQSKLNDLLETYRDGLQEAFDAGASTMSAVSEIVTPYDLPYQAKAALCNYVPKLRKTYSAKELDDSHPIRLTNQAAKFDHS
AERDYEFTWWVPRPGRGTNFWIPLRINPEQEGLWHDLVSEDAKAGEIRLQKHRKNWVLHVTVEYPVEEPATDGDATHIGLDIGETALITGCALKDGSPTD
PFVCSGSRAKHLRKEMHTTLKRLQERDASEWRIEDRFSHYQNALTDIVEKVSRQAVEYAQQFENPVLVMEDLTYIRERLDYGKYMNRRLHSWAFARLQGR
IEDKATEAGIPVEYVNPAYTSQTCHSCHRIGRRDSQAEFRCPNDDCHVSTFQADINASANIARRVDPWGESVPLDKAERDDSPRDGSSCDTATTHRETSV
PAQMTLTAYEESKPSASDY

 

Blast result :
Comments
ISHal1 is 89% (TnpA : the transposase) and 82% (TnpB) aa similar to ISNph5.
References
1] Hartman, A.L., Norais, C., Badger, J.H., Delmas, S., Haldenby, S., Madupu, R., Robinson, J., Khouri, H., Ren, Q., Lowe, T.M., Maupin-Furlow, J., Pohlschroder, M., Daniels, C., Pfeiffer, F.,
Allers, T., Eisen, J.A. (2010) PLoS ONE 5, E9605-E9605