ISHaha4
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HE717023 | ND | Halobacillus halophilus | Halobacillus halophilus DSM 2266 |
DNA section
IS Length : 1271 bp
Ends
IR Length : 30/38
IRL : TAGAGTGACCTAGAAAAATGAGACGTAATAAAACACCTTCAAAGTTGTAA
IRR : TGGAGTGCCACAGTAAAATAAGACTCAATAAAACACCTAGGCTGCCATTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CACATGGTCT | ATT | CCATGTGGAC | 3 |
| CGGTTCCTCA | TGAA | GGAATCCGGG | 4 |
| TCCCTAATGT | TTAT | AAACATCAGG | 4 |
| CCTCGTGTCA | GCTC | ATGCACACGA | 4 |
| ACCCTCTACT | TAT | AAAAGTAGAG | 3 |
| TCCTTCTGAG | GTA/GTG | AACACAAAGG | 7 |
DNA sequence
TAGAGTGACCTAGAAAAATGAGACGTAATAAAACACCTTCAAAGTTGTAATACTACACTTGGAGGTGTTTTTCTTATGGGTAAAAAAGTTCACTATCCCG
AAGAAGTAAAAAAGAAAGTAGTTCAATTAAAATTAGAAGGGAATCACACGAATCAAGAGTTGATGGACATGTTTGGAATTAAGAATAAGTCCCAGATTAA
AACGTGGATGAAATGGTTTAGAAATGGGGAAAACCACCGGTTTGCTCAGCCTATAGGGAAACAATATTCTCACGGGAAAGGGCCGGAAGACGAGGATGAA
ATGACCCAACTGAAGAAAAAGATTGCCTATTATGATATGAGAGAAGAACTGTTGGAAAAGTACCAGGAAATCGAAAGGAAGTGGTTCCAGAAGTATTCAT
CCAAATCGTAGAAACATTTAAGCACAAGTATCCTGTGAAAGATATTTGTGAATGCTTTGGTATAGCTAAAGCCACCTATTATCGCTGGCGGGGAAAGTCA
TGGGGACCGTCGGTCCTGGAGAAGAAGATCATTGAGCTATGTGAAGATCTAAAATTTAGAGTGGGACACCGTATGGTCCGTGACCTCTTACGTAAGGAAT
ACCAAATCAAACTCAATCGAAATACCGTTCAAAAAATCATGCAGAAATACCATATTCAATGTCGCGTAAAACCAAAGAGACAGTGTTATATCGCAGGGGA
AAGTAAGATTGTGGTACCGAATTATTTAGAGAGAAACTTTACGTCTACGAGACCTAATGAAAAATGGGTAACAGATATTACCTATCTTCCTTACGGAGAG
AAAATGTTATATTTATCAACGATTATGGACTTATACAACAACGAAATCCTCGCGTACACGATCAGTGATAAACAAGATACGTCCTTCGTATTAGATACGT
TGAGGAAAGCTTGCGAAGGACGTGACGTCTATGAAGTGATCCTTCATAGCGATCAAGGTTCGCAGTACACGTCCTATGAATTTCAAGAAGAGGCCAAAAA
AAGAGGCATTATCACAAGCATGTCCCGACGGGGGAACTGCTTTGATAATGCCGTCATCGAATCCTTCCACTCCACGATAAAATCGGAAGAATTCAGTCCT
CTGGACCGAGGAAAAATAGATCATCATACCGTACGCTCAAAAATCGATACGTACATGTATTACTACAATTATTGTCGACCTTTTCAAAAACTAAACTGCC
AAACTCCAATTGAATTCAGAGTAATGGCAGCCTAGGTGTTTTATTGAGTCTTATTTTACTGTGGCACTCCA
AAGAAGTAAAAAAGAAAGTAGTTCAATTAAAATTAGAAGGGAATCACACGAATCAAGAGTTGATGGACATGTTTGGAATTAAGAATAAGTCCCAGATTAA
AACGTGGATGAAATGGTTTAGAAATGGGGAAAACCACCGGTTTGCTCAGCCTATAGGGAAACAATATTCTCACGGGAAAGGGCCGGAAGACGAGGATGAA
ATGACCCAACTGAAGAAAAAGATTGCCTATTATGATATGAGAGAAGAACTGTTGGAAAAGTACCAGGAAATCGAAAGGAAGTGGTTCCAGAAGTATTCAT
CCAAATCGTAGAAACATTTAAGCACAAGTATCCTGTGAAAGATATTTGTGAATGCTTTGGTATAGCTAAAGCCACCTATTATCGCTGGCGGGGAAAGTCA
TGGGGACCGTCGGTCCTGGAGAAGAAGATCATTGAGCTATGTGAAGATCTAAAATTTAGAGTGGGACACCGTATGGTCCGTGACCTCTTACGTAAGGAAT
ACCAAATCAAACTCAATCGAAATACCGTTCAAAAAATCATGCAGAAATACCATATTCAATGTCGCGTAAAACCAAAGAGACAGTGTTATATCGCAGGGGA
AAGTAAGATTGTGGTACCGAATTATTTAGAGAGAAACTTTACGTCTACGAGACCTAATGAAAAATGGGTAACAGATATTACCTATCTTCCTTACGGAGAG
AAAATGTTATATTTATCAACGATTATGGACTTATACAACAACGAAATCCTCGCGTACACGATCAGTGATAAACAAGATACGTCCTTCGTATTAGATACGT
TGAGGAAAGCTTGCGAAGGACGTGACGTCTATGAAGTGATCCTTCATAGCGATCAAGGTTCGCAGTACACGTCCTATGAATTTCAAGAAGAGGCCAAAAA
AAGAGGCATTATCACAAGCATGTCCCGACGGGGGAACTGCTTTGATAATGCCGTCATCGAATCCTTCCACTCCACGATAAAATCGGAAGAATTCAGTCCT
CTGGACCGAGGAAAAATAGATCATCATACCGTACGCTCAAAAATCGATACGTACATGTATTACTACAATTATTGTCGACCTTTTCAAAAACTAAACTGCC
AAACTCCAATTGAATTCAGAGTAATGGCAGCCTAGGTGTTTTATTGAGTCTTATTTTACTGTGGCACTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 336 bp | 111 aa | 76 | 411 | + | No |
Description : First part of the transposase
ORF sequence :
MGKKVHYPEEVKKKVVQLKLEGNHTNQELMDMFGIKNKSQIKTWMKWFRNGENHRFAQPIGKQYSHGKGPEDEDEMTQLKKKIAYYDMREELLEKYQEIE
RKWFQKYSSKS
RKWFQKYSSKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 900 bp | 299 aa | 336 | 1235 | + | No |
Description : Second part of the transposase
ORF sequence :
YERRTVGKVPGNRKEVVPEVFIQIVETFKHKYPVKDICECFGIAKATYYRWRGKSWGPSVLEKKIIELCEDLKFRVGHRMVRDLLRKEYQIKLNRNTVQK
IMQKYHIQCRVKPKRQCYIAGESKIVVPNYLERNFTSTRPNEKWVTDITYLPYGEKMLYLSTIMDLYNNEILAYTISDKQDTSFVLDTLRKACEGRDVYE
VILHSDQGSQYTSYEFQEEAKKRGIITSMSRRGNCFDNAVIESFHSTIKSEEFSPLDRGKIDHHTVRSKIDTYMYYYNYCRPFQKLNCQTPIEFRVMAA
IMQKYHIQCRVKPKRQCYIAGESKIVVPNYLERNFTSTRPNEKWVTDITYLPYGEKMLYLSTIMDLYNNEILAYTISDKQDTSFVLDTLRKACEGRDVYE
VILHSDQGSQYTSYEFQEEAKKRGIITSMSRRGNCFDNAVIESFHSTIKSEEFSPLDRGKIDHHTVRSKIDTYMYYYNYCRPFQKLNCQTPIEFRVMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 76 | 1235 | + | Yes |
Chemistry : DDE
ORF sequence :
MGKKVHYPEEVKKKVVQLKLEGNHTNQELMDMFGIKNKSQIKTWMKWFRNGENHRFAQPIGKQYSHGKGPEDEDEMTQLKKKIAYYDMREELLEKVPGNR
KEVVPEVFIQIVETFKHKYPVKDICECFGIAKATYYRWRGKSWGPSVLEKKIIELCEDLKFRVGHRMVRDLLRKEYQIKLNRNTVQKIMQKYHIQCRVKP
KRQCYIAGESKIVVPNYLERNFTSTRPNEKWVTDITYLPYGEKMLYLSTIMDLYNNEILAYTISDKQDTSFVLDTLRKACEGRDVYEVILHSDQGSQYTS
YEFQEEAKKRGIITSMSRRGNCFDNAVIESFHSTIKSEEFSPLDRGKIDHHTVRSKIDTYMYYYNYCRPFQKLNCQTPIEFRVMAA
KEVVPEVFIQIVETFKHKYPVKDICECFGIAKATYYRWRGKSWGPSVLEKKIIELCEDLKFRVGHRMVRDLLRKEYQIKLNRNTVQKIMQKYHIQCRVKP
KRQCYIAGESKIVVPNYLERNFTSTRPNEKWVTDITYLPYGEKMLYLSTIMDLYNNEILAYTISDKQDTSFVLDTLRKACEGRDVYEVILHSDQGSQYTS
YEFQEEAKKRGIITSMSRRGNCFDNAVIESFHSTIKSEEFSPLDRGKIDHHTVRSKIDTYMYYYNYCRPFQKLNCQTPIEFRVMAA
Blast result :
Comments
ISHaha4 is 66% aa similar to ISErh1.
The third ORF is a ORFAB transposase reconstructed to represent a probable -1 ribosome slippage.
The third ORF is a ORFAB transposase reconstructed to represent a probable -1 ribosome slippage.
References