ISfinder Mirror Site

ISEc49

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_004431	ND	Escherichia coli	Escherichia coli CFT073 Shigella boydii CDC 3083-94

DNA section

IS Length : 2766 bp

Ends

IR Length : 19/27

IRL : GTGTCGTTACGGGGAAGTCATCTTCACAGGGATCTTCGGTCAGTTAGTAT
IRR : GTAAGCTTCCGGGGAACTCATATTGACGCGTTTTTCAGGCGCGCGTCATC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGATCGTTCG	TATGCGCC	GCCGATGACG	8
AATTTGCCGA	AATTTTGA	TAATCAGTTC	8

DNA sequence

GTGTCGTTACGGGGAAGTCATCTTCACAGGGATCTTCGGTCAGTTAGTATTACCGACATCCATTCACCAACTTACCGGAGATAATCGATGTCAAAACCCC
GATGGACCATTGAACAGAAAAGGCAGCACGTTGCTGCCTGGCGCGCCAGTGGTCTTACACGTCAGCAATACTGTGAACTCAACGATATTCCCTTCACATC
ACTTCGCGAATGGCCGCAGGATGTCGCAAAAGCTGAACGCCGGGCAAATGAACCGACCGTTCTTCCCGTTCATATTGCGCCACCACTGCATGCTGATGTA
CCACAGCCCGTCACGAATGAGCCAGTCACGCTCTTCCTTCCCGGTGGAGTACGGATGTGCTGTCAGCCGTCACAACTTACCGATGTCTTCAGGGCACTCA
AATATGCTCAGTCCTGATAATGTCTTCATCGCAATTAAACCTGTTGACATGCGCCGGGGCATCGACTCACTGACGCAGTATATACAGGATGAACTCCGGT
CGACATGGCATGAGGGAGCCGCCTTCGTCTTTGTTAACAAAGCCCGTTCGCGTATCAAAGTTCTCCGGTGGGATAAACACGGGGTGTGGTTGTGTACCCG
CCGTCTGCACAAAGGCAGCTTCCGCTGGCCACGTGCAAATGACGCTGCCTGGCACCTCACTCCCGACGAGTTTAACTGGCTGATTGCCGGTGTTGACTGG
CAGCAGGTTAAGGGACATGACCTGACGAAATGGGTCTGGCAGAATGAACCTGAACTGCGCCCTGAAAACACGCAAAATACACTGCTAACTCAGTGAAAAA
TAATGAATATCCGTATCTGGAGTGGTATACTCCCCTGTATGGATATCTCCGCTCTCAACACCACGAATGACATCGAAAAACTGCGTGCTATGGCACTTGC
CATGGTACAAGAAGTCATGTCGGAGAATGCCGAAAAAGAGCGGGAATTACTGGAGAAAAGCCGGCGCATCCAGCTTCTGGAAGAAATGCTGAAACTGGTT
CGTCAACAGCGCTTCGGAAAAAAATGTGGAACGCTGGCTGGTATGCAACGCTCCCTGTTCGAAGAGGATGTTGATGCCGATATCGCCGCGCTTACCGCAC
ATCTGGATAAACTGCTCCCGCAATCCCCTGAAGAAGACGAAAAAGCGTCCCGTTCACGCCCGATACGCAAACCCTTACCGGTTCATCTTCCACGGGTGGA
AAAAATTATCCAGCCGGACACTGACCATTGCCCTGAATGTGACGAGCCGCTGCACTATATCCGCGATGCGGTGAGTGAAAAGCTGGAGTATATTCCCGCT
CACTTTGTGGTGAACCGTTATGTCCGTCCACAATACAGTTGTCCCTGTTGCCAGAAGGTGTTCAGCGGTGAAATGCCGGCACATATCCTCCCGAAAAGTG
CCGTTGAGCCATCAGTCATCGCACAGGTGATCATCAATAAATACGGTGACCACCTGCCTCTGTATCGCCAGCAACAGGTCTTTGCCCGTTCAGATGTCGG
GCTGCCCGTCAGTTCGATGGCTGACATGGTTGGCGCGGCGGGTGCCGCATTATCTCCCCTGGCGGCGTTACTCCATCGCGAGTTGATAAACCGTCCGGTG
GTGCATGCAGATGAGACTACCCTGAAGATCCTGAACACGAAGAAAGGCGGTAAATCCTGCTCCGGTTATCTGTGGGCATACGTCAGTGGAGAAAGGACGG
GACCGTCAGTTGTGTGCTTCGACTGCCGGACCGGACGTAGCCATGAGTATCCTGAAAACTGGCTTCAGGGCTGGGGCGGGACGCTGGTTGTCGACGGACA
TAAAGCTTACCGGACTCTGGCAAACAAAGTGCCGGAGATCACGCTGGCCGGATGCTGGGCCCATGCCCGCAGGGGCTTCGCCGACCTGTATAAAATCAGT
AAAGATCCACGGGCTGCCATAGCCGTGAAGAAAATCGCGGGGTTGTACCGTCTTGAGAAGAAGATCAGTAGCCGCCCCGTGGAAAAAATCCGCCAGTGGC
GACAGCGTTATGCCCGTCCGATACTGGAAGAACTGTGGTCATGGCTTGAAGAGCAGGAACCGCAATGTTCTCCGGGAAGGGCATTACACAAAGCCATTGC
CTATGCGCTGTCTCATCGCGTGGAACTGAGCCGCTTCCTGGAAGATGGTGCGGTGCCGCTGGATAATAATGTGTGTGAACGGGCCATCAAAAACGTGGTT
CTGGGCAGAAAATCGTGGCTGTTCGCCGGTTCGCAGATGGCGGGAGAACGCGCCGCGCAAATAATGAGCTTGCTGGAAACCGCGAAACGCAACGGTCTGG
AGCCGCATGCCTGGTTGACAGACGTCCTGATGCGTCTGCCGGAGTGGCCGGAGGAGCGACTGGCAGAGTTGCTGCCTCTTGAGGGATTTACCTTCTCCGG
GTGAGTGATACCTGCCGTCAGGTGTTCGTGCACCGGGCCATAACCTGCAGTCGGGAGTTGAACTCCTGACGGCAGGAAATGAGCCAGACCAGCAACCACG
CGGGCTGACAGCGAATAAGAACCTGAATCAGCCCGGAGTCATTCCCGGGTCAGTACATCATCACCTGCTATTAATCCCCGAGTCAGTACTTCGCGGCCTG
ACGCGATCACCCTACTCAGTACTTCAGAGCCCGCCTCCGGTACCTGCCTGGCGATCGCCCCTGTGCAACAAAATAAATCAACCGTTATCAAACCACGATC
GTTCGTATGCGCCGCCGATGACGCGCGCCTGAAAAACGCGTCAATATGAGTTCCCCGGAAGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
330 bp	109 aa	88	417	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQDVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDV
FRALKYAQS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
393 bp	130 aa	404	796	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFVNKARSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQ
QVKGHDLTKWVWQNEPELRPENTQNTLLTQ

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1602 bp	533 aa	803	2404	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAEKERELLEKSRRIQLLEEMLKLVRQQRFGKKCGTLAGMQRSLFEEDVDADIAALTAH
LDKLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKIIQPDTDHCPECDEPLHYIRDAVSEKLEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSA
VEPSVIAQVIINKYGDHLPLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKILNTKKGGKSCSGYLWAYVSGERTG
PSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHKAYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRPVEKIRQWR
QRYARPILEELWSWLEEQEPQCSPGRALHKAIAYALSHRVELSRFLEDGAVPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLE
PHAWLTDVLMRLPEWPEERLAELLPLEGFTFSG

Blast result :

Comments

ISEc49 is 58%(orfA) aa similar to ISVsp4, 72%(orfB) and 58%(orfC) aa similar to ISAzo15.

References

1] ISfinder annotation (2012)
2] Welch,R.A., Burland,V., Plunkett,G. III, Redford,P., Roesch,P., Rasko,D., Buckles,E.L., Liou,S.R., Boutin,A., Hackett,J., Stroud,D., Mayhew,G.F., Rose,D.J., Zhou,S., Schwartz,D.C., Perna,N.T., Mobley,H.L., Donnenberg,M.S. and Blattner,F.R. (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 17020-17024