ISfinder Mirror Site

ISMmu1

Family IS200/IS605
Group IS605

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_GG697141	ND	Mitsuokella multacida	Mitsuokella multacida DSM 20544

DNA section

IS Length : 2023 bp

Ends

Left end : CAATTAAAATGCGGCCAGGAATCTTCTCACTTCAGTCAGGAGAGGAATGGCCGCAACGGTAGTCACTTGGAATACTGGTTCTGAATGTATTTTGCGACTA II struct. : Yes
Right end : ACCTCGCAAGACTGCGAATGCAGTAAGCCTGGCCTATCTAAGCAAATTGAGGTATCGAAAGATTCCCTCAAGCTCGCGACTTCAGTCGCGGGTAGTTGAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
TCCATAGATTCTG	TTCT	AAAGAGGCCC	TGAC
GAATCCTTCTAGG	TTTT	TCTTATCAGG	TGAC

DNA sequence

CAATTAAAATGCGGCCAGGAATCTTCTCACTTCAGTCAGGAGAGGAATGGCCGCAACGGTAGTCACTTGGAATACTGGTTCTGAATGTATTTTGCGACTA
CTTCCTTGCTCATGTTGCCAAGTGTGCTCATATAGTAGCTGCGTGACCAGATATGACCACCCCAGAATTTATCATGCTTGATCTCGGGATGTTTTTTTAG
GAACAGTCTGGCACTGCCGCCTTTGAGTGCCTTGACGATGTTTGTCGGCGTATACTTTGGCTTGAAGCTGATGAGCATGTGCACATGCTCTGGCATGACT
TCCAGCTCTTCGACGGTCACATCGTTCAGCTGCGCGATGCGCAGCAAGATATCCTTCATCTCCTGCACGAGCTCCGGTGTCGTGAAGGCTGGGTTCCGAT
ACTTCGTCACCCAGATCAGATGAAAGTGGAAATTGTAGACGTATCCACGCTCATAGATTGCACCTGGGATGCGCTCTTTTCGTTTCATGTGTATCACACT
CCTTTCTTTTTTATTATAGCGATATATTGCTTGTTAGGCAATCTCATATGCTGTATAATAGAAGTAGGAAGGAGGCGAATAAAATGGAACATAAGAAGAT
GGCGGATTATGCCTATCATTACGGTCTGAAAGTCCGCATCTACCCCAGCCGGCAACAGAAAGAGATGATTCGACGCAATGCGGATGCGGCACGCTGCATC
TACAATGAACATGTCGCTGTGAACAAGGAGATCGCACGATTCGGCAGACGGAGCATCTACATCGACTTCATTGAGAAGCGCATCGAGGCACTGCGCGAGC
GAATCCGCACGGTAACAGCATTGAAAGACCGCTATCCATGGCTCCGGCATCCCGATATCGATGCGCAGTCCATTGCCAATGCACGGCAGAACTACCAGCA
TGCTTGGAAGCAGTTCCGTGCCGTCCATCATGCTGGTGTGCCGAACTTCCACAAGAAAACGTCCGTCGAGTCGTATCAGACGAATCCGCATTACAGCAAG
GGTACGGTGATTTCGCTGTTTACCAGTAACGCCCGCTTCCTCGACAAGCATCATCTGATGCTGCCCAAGCTCAAACGCATCCGCTTCCAAGGTTCGCGTA
AAATACTGGACCGACTTTTTGCCATGAAGGAAGTCCGCATCGGAACGATTACCATCACGAAAGACAGCCTGCACCGCTACTTTGCGTCATTCCAACTCGG
CTCCGATGAACCATTCGTGAAAGCCAGCCCGAAAACAAAGGCAGAGATTGGCATCGACCTGAACATCGAGAACTTCTATGCCGACTCGAATGGCGAGATT
GTGGCCAATCCTCGTTATTACCGCAGGCAGAAACGACGGCTGGCCAACGCACAGCGCAAGCTGAGCCGTCGGATTGTCCGTGCCAAGAAAGAGCATCGCT
CGCTCCGTGAGGCAAAGAACGTCGAAAAACAGCGCTTGCTCGTCGCCAGGCTCACGGCACAAGTCATGCAGCGCCGCAAAGATTTCCTGCACCGCACTTC
CGCGACACTGATCAAGAACCACGATCTGGTCGCAGCAGAAGAACTACGGAGCAAGAATCTGCTGAAGAACCATGCCCTGGCTATGAGCATCCAAGACAAT
GGCTGGCGGATGTTCCTTAGTATGCTGACTTATAAGGCAGCACTGTACGGCAAGAACTTCGTCACCGTCGACCCGCGGAATACGACGCAAACCTGCCACG
CTTGTGGCCATATCATGAGCGGTAAAGAGAAACTGACGCTCAAGGACCGCGAGTGGATCTGCCCGAAATGTGGGATACACCACCTTCGCGATGTGAATGC
GGCCAAGAACATCCTAGCGCGAGCCAAAGCAAAGCTCGTATCCATGCATTGATGCGAACCTACCTTGTCCGTTTGGCAACTTGCGGACGCTAATGGCTTT
GGTAATTACCGGACCTCTGCTGTACCTCGCAAGACTGCGAATGCAGTAAGCCTGGCCTATCTAAGCAAATTGAGGTATCGAAAGATTCCCTCAAGCTCGC
GACTTCAGTCGCGGGTAGTTGAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
426 bp	141 aa	488	63	-	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKRKERIPGAIYERGYVYNFHFHLIWVTKYRNPAFTTPELVQEMKDILLRIAQLNDVTVEELEVMPEHVHMLISFKPKYTPTNIVKALKGGSARLFLKKH
PEIKHDKFWGGHIWSRSYYMSTLGNMSKEVVAKYIQNQYSK

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1305 bp	434 aa	548	1852	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MLYNRSRKEANKMEHKKMADYAYHYGLKVRIYPSRQQKEMIRRNADAARCIYNEHVAVNKEIARFGRRSIYIDFIEKRIEALRERIRTVTALKDRYPWLR
HPDIDAQSIANARQNYQHAWKQFRAVHHAGVPNFHKKTSVESYQTNPHYSKGTVISLFTSNARFLDKHHLMLPKLKRIRFQGSRKILDRLFAMKEVRIGT
ITITKDSLHRYFASFQLGSDEPFVKASPKTKAEIGIDLNIENFYADSNGEIVANPRYYRRQKRRLANAQRKLSRRIVRAKKEHRSLREAKNVEKQRLLVA
RLTAQVMQRRKDFLHRTSATLIKNHDLVAAEELRSKNLLKNHALAMSIQDNGWRMFLSMLTYKAALYGKNFVTVDPRNTTQTCHACGHIMSGKEKLTLKD
REWICPKCGIHHLRDVNAAKNILARAKAKLVSMH

Blast result :

Comments

ISMmu1 is 79% (TnpA : the transposase) aa and 67% (ORFB) aa similar to ISLjo5.

References

1] ISfinder annotation (2012)
2] Weinstock,G., Sodergren,E., Clifton,S., Fulton,L., Fulton,B., Courtney,L., Fronick,C., Harrison,M., Strong,C., Farmer,C., Delahaunty,K., Markovic,C., Hall,O., Minx,P., Tomlinson,C., Mitreva,M., Nelson,J., Hou,S., Wollam,A., Pepin,K.H., Johnson,M., Bhonagiri,V., Nash,W.E., Warren,W., Chinwalla,A., Mardis,E.R. and Wilson,R.K. (2008) Direct submission GenBank.