ISSth1b
- Family IS3
- Group IS150
Isoform Synonym(s) ISSth1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| FR875178 | ND | Streptococcus thermophilus | Streptococcus thermophilus LMD-9 Streptococcus thermophilus JIM 8232 Streptococcus thermophilus LMG 18311 Streptococcus thermophilus CNRZ1066 Streptococcus thermophilus ND03 |
DNA section
IS Length : 1684 bp
Ends
IR Length : 29/39
IRL : TGATATACTCCCCTTATAGTGGACAGTGAAAAAATAAAAATTTTCACTAG
IRR : TGAACAGCTCCCTGTCAAGTGGACAATGAAATAATAAAATATTAGGCGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACTCACATTC | AAC | TTCATGATTG | 3 |
| CCCTCACCCG | ATA | TGAGTGAGGG | 3 |
| GCTAGCCTAT | TTGAAAGGAC | 0 |
DNA sequence
TGATATACTCCCCTTATAGTGGACAGTGAAAAAATAAAAATTTTCACTAGAAACTATAAGGGGAGTCTTATTATGTCCAAAAGAAGTCCAAAATCTGTAT
CTGAGAAACTAGAAATTGTTCTGCTTCACTTGGAAGAAGGTAAATCACTTAGTTGGTTAACTAGAAACCAAGGTATCTCTAAAGACACCCTATCGAACTG
GGTTCGGAAGTACAAAGAAGCTGGTGTTGATGGGCTAGAGGAAAGCCGTCAATGGAAGAAGTATAGTAAGGAACTAAAGGAACAAGCTGTTTCCGACTAT
CTTAATGGCTTGGGAAGTCTCAAAGATCTGACCAAAAAATATGGAATTTCTGACCCTTATGTTCTCAGATCATGGATAAAAAGTTATACTAGTGGTAAAG
AACTGAAAGCTACTAGTAAAGGAATGAGACGCATGAAGCAAGGACGCAAGACAACATTTGAAGAACGAATTGAGATTGTCAATTTTACCCTTGCCCACGA
GAAAGATTACCAAGGGGCTGTTGAGAAGTATGGTATTTCCTACCAGCAGATCTATTCTTGGGTCAGAAAGTTCGAGAAGGACGGTTCTAACGGTCTCCTA
GATCGTCGTGGAAAAGGTCTTACAAGTAAACCAAACCTTACTCCAGAAGAAGAGTTACGCCTCAAAATCAAGCAACAGGAAGAGCGGATTAAGTATCTTG
AAATGGAGAACGGCCTGCTAAAAAAGTTAGAAGAAATCAAACGACGAAACCGACGGTAAGACTTGGTCGGCACTTGGAAACCTTCCAAGCGATTAAAGAA
TATGCGGATGAGTATGAAGAGGTTTCTATCAGCCACCTGTGCCATATTCTAAAGGTATCTCGCTCAGGCTATTACAAGTGGTTACAACATCAAGAAACAA
CTTCTGAACAGGAAAATTTAGGCTTGATGGATATCATCAAGAAACTTCATAGCCAGCATAATGGTATTCTTGGTTATCGTCGTATGACGCTATTTGTCAA
TCGCAAGCTTGAAACAAACTACAACAAGAAGCGGATTCGACGCTTGATGCACATTCTAGGTCTACGTTCCATTATCAGAAGAGCCAAGGGCTATTGTACT
AAAACTAGCTTTGTCAATGTAGAGGACAACATTCTCAATCGTAATTTTACAGCCACTGCTCCAAATCAGAAATGGTGTACAGATGTGACTTTCTTGAAGT
ACGGTTTCAGCTGTAAAGCCTATTTGAGTGCTATTAAGGATCTTTACGACGGCTCAATTGTCGCCTATGTAGTTGGTCAATTTAATGATAACGAATTGGT
ATTGGAAACACTTCGTAAAGCACAAAAAGCTAATCCTAATGCGACACCATTAATTCACAGCGACCGAGGTTCGCAGTATACTTCGAAAGATTATTACCGT
TTAACTACCCAGTATCAGATGACCCGCTCCATGTCTCGTGTTGGTAAGTGTATTGACAATGCACCAATTGAGAGTTTCTTTGGGCACTTTAAGACGGAGT
GCTATGATTTGAAGAAGTATAAGACTTTTGAGGAGCTAGTTTCAGATATTGATGCCTACATCTATTTTTACAATCATCAACGTTTTCAAGAGCGCAATAA
CGGCCTTGCCCCTCTTGAAATGAGGAACAAGGCCGTCGCCTAATATTTTATTATTTCATTGTCCACTTGACAGGGAGCTGTTCA
CTGAGAAACTAGAAATTGTTCTGCTTCACTTGGAAGAAGGTAAATCACTTAGTTGGTTAACTAGAAACCAAGGTATCTCTAAAGACACCCTATCGAACTG
GGTTCGGAAGTACAAAGAAGCTGGTGTTGATGGGCTAGAGGAAAGCCGTCAATGGAAGAAGTATAGTAAGGAACTAAAGGAACAAGCTGTTTCCGACTAT
CTTAATGGCTTGGGAAGTCTCAAAGATCTGACCAAAAAATATGGAATTTCTGACCCTTATGTTCTCAGATCATGGATAAAAAGTTATACTAGTGGTAAAG
AACTGAAAGCTACTAGTAAAGGAATGAGACGCATGAAGCAAGGACGCAAGACAACATTTGAAGAACGAATTGAGATTGTCAATTTTACCCTTGCCCACGA
GAAAGATTACCAAGGGGCTGTTGAGAAGTATGGTATTTCCTACCAGCAGATCTATTCTTGGGTCAGAAAGTTCGAGAAGGACGGTTCTAACGGTCTCCTA
GATCGTCGTGGAAAAGGTCTTACAAGTAAACCAAACCTTACTCCAGAAGAAGAGTTACGCCTCAAAATCAAGCAACAGGAAGAGCGGATTAAGTATCTTG
AAATGGAGAACGGCCTGCTAAAAAAGTTAGAAGAAATCAAACGACGAAACCGACGGTAAGACTTGGTCGGCACTTGGAAACCTTCCAAGCGATTAAAGAA
TATGCGGATGAGTATGAAGAGGTTTCTATCAGCCACCTGTGCCATATTCTAAAGGTATCTCGCTCAGGCTATTACAAGTGGTTACAACATCAAGAAACAA
CTTCTGAACAGGAAAATTTAGGCTTGATGGATATCATCAAGAAACTTCATAGCCAGCATAATGGTATTCTTGGTTATCGTCGTATGACGCTATTTGTCAA
TCGCAAGCTTGAAACAAACTACAACAAGAAGCGGATTCGACGCTTGATGCACATTCTAGGTCTACGTTCCATTATCAGAAGAGCCAAGGGCTATTGTACT
AAAACTAGCTTTGTCAATGTAGAGGACAACATTCTCAATCGTAATTTTACAGCCACTGCTCCAAATCAGAAATGGTGTACAGATGTGACTTTCTTGAAGT
ACGGTTTCAGCTGTAAAGCCTATTTGAGTGCTATTAAGGATCTTTACGACGGCTCAATTGTCGCCTATGTAGTTGGTCAATTTAATGATAACGAATTGGT
ATTGGAAACACTTCGTAAAGCACAAAAAGCTAATCCTAATGCGACACCATTAATTCACAGCGACCGAGGTTCGCAGTATACTTCGAAAGATTATTACCGT
TTAACTACCCAGTATCAGATGACCCGCTCCATGTCTCGTGTTGGTAAGTGTATTGACAATGCACCAATTGAGAGTTTCTTTGGGCACTTTAAGACGGAGT
GCTATGATTTGAAGAAGTATAAGACTTTTGAGGAGCTAGTTTCAGATATTGATGCCTACATCTATTTTTACAATCATCAACGTTTTCAAGAGCGCAATAA
CGGCCTTGCCCCTCTTGAAATGAGGAACAAGGCCGTCGCCTAATATTTTATTATTTCATTGTCCACTTGACAGGGAGCTGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 687 bp | 228 aa | 73 | 759 | + | No |
Description : First part of the transposase
ORF sequence :
MSKRSPKSVSEKLEIVLLHLEEGKSLSWLTRNQGISKDTLSNWVRKYKEAGVDGLEESRQWKKYSKELKEQAVSDYLNGLGSLKDLTKKYGISDPYVLRS
WIKSYTSGKELKATSKGMRRMKQGRKTTFEERIEIVNFTLAHEKDYQGAVEKYGISYQQIYSWVRKFEKDGSNGLLDRRGKGLTSKPNLTPEEELRLKIK
QQEERIKYLEMENGLLKKLEEIKRRNRR
WIKSYTSGKELKATSKGMRRMKQGRKTTFEERIEIVNFTLAHEKDYQGAVEKYGISYQQIYSWVRKFEKDGSNGLLDRRGKGLTSKPNLTPEEELRLKIK
QQEERIKYLEMENGLLKKLEEIKRRNRR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 942 bp | 313 aa | 702 | 1643 | + | No |
Description : Second part of the transposase
ORF sequence :
NGERPAKKVRRNQTTKPTVRLGRHLETFQAIKEYADEYEEVSISHLCHILKVSRSGYYKWLQHQETTSEQENLGLMDIIKKLHSQHNGILGYRRMTLFVN
RKLETNYNKKRIRRLMHILGLRSIIRRAKGYCTKTSFVNVEDNILNRNFTATAPNQKWCTDVTFLKYGFSCKAYLSAIKDLYDGSIVAYVVGQFNDNELV
LETLRKAQKANPNATPLIHSDRGSQYTSKDYYRLTTQYQMTRSMSRVGKCIDNAPIESFFGHFKTECYDLKKYKTFEELVSDIDAYIYFYNHQRFQERNN
GLAPLEMRNKAVA
RKLETNYNKKRIRRLMHILGLRSIIRRAKGYCTKTSFVNVEDNILNRNFTATAPNQKWCTDVTFLKYGFSCKAYLSAIKDLYDGSIVAYVVGQFNDNELV
LETLRKAQKANPNATPLIHSDRGSQYTSKDYYRLTTQYQMTRSMSRVGKCIDNAPIESFFGHFKTECYDLKKYKTFEELVSDIDAYIYFYNHQRFQERNN
GLAPLEMRNKAVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1571 bp | 523 aa | 73 | 1643 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKRSPKSVSEKLEIVLLHLEEGKSLSWLTRNQGISKDTLSNWVRKYKEAGVDGLEESRQWKKYSKELKEQAVSDYLNGLGSLKDLTKKYGISDPYVLRS
WIKSYTSGKELKATSKGMRRMKQGRKTTFEERIEIVNFTLAHEKDYQGAVEKYGISYQQIYSWVRKFEKDGSNGLLDRRGKGLTSKPNLTPEEELRLKIK
QQEERIKYLEMENGLLKKVRRNQTTKPTVRLGRHLETFQAIKEYADEYEEVSISHLCHILKVSRSGYYKWLQHQETTSEQENLGLMDIIKKLHSQHNGIL
GYRRMTLFVNRKLETNYNKKRIRRLMHILGLRSIIRRAKGYCTKTSFVNVEDNILNRNFTATAPNQKWCTDVTFLKYGFSCKAYLSAIKDLYDGSIVAYV
VGQFNDNELVLETLRKAQKANPNATPLIHSDRGSQYTSKDYYRLTTQYQMTRSMSRVGKCIDNAPIESFFGHFKTECYDLKKYKTFEELVSDIDAYIYFY
NHQRFQERNNGLAPLEMRNKAVA
WIKSYTSGKELKATSKGMRRMKQGRKTTFEERIEIVNFTLAHEKDYQGAVEKYGISYQQIYSWVRKFEKDGSNGLLDRRGKGLTSKPNLTPEEELRLKIK
QQEERIKYLEMENGLLKKVRRNQTTKPTVRLGRHLETFQAIKEYADEYEEVSISHLCHILKVSRSGYYKWLQHQETTSEQENLGLMDIIKKLHSQHNGIL
GYRRMTLFVNRKLETNYNKKRIRRLMHILGLRSIIRRAKGYCTKTSFVNVEDNILNRNFTATAPNQKWCTDVTFLKYGFSCKAYLSAIKDLYDGSIVAYV
VGQFNDNELVLETLRKAQKANPNATPLIHSDRGSQYTSKDYYRLTTQYQMTRSMSRVGKCIDNAPIESFFGHFKTECYDLKKYKTFEELVSDIDAYIYFY
NHQRFQERNNGLAPLEMRNKAVA
Blast result :
Comments
ISSth1b is 57% aa similar to ISSau2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
This element was renamed because the same name was used for two different element (ISSth1).
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
This element was renamed because the same name was used for two different element (ISSth1).
References
1] Submitted by ISfinder (2011).
2] Loux,V. (2011) Direct submission to GenBank.
2] Loux,V. (2011) Direct submission to GenBank.