ISVal2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HM362422 | ND | Vibrio alginolyticus | Vibrio alginolyticus X213 |
DNA section
IS Length : 1243 bp
Ends
IR Length : 27
IRL : TGATCTGCCCCAACAGTTTTGGACACTGAGTTAAGTGAGTACAATCACTA
IRR : TGATCTGCTCCAGCCAAACTGGACACTTCATTAAGCGACATTTTTCTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATAGCAAAC | CTATCTGAAA | 0 |
DNA sequence
TGATCTGCCCCAACAGTTTTGGACACTGAGTTAAGTGAGTACAATCACTAACGAGGTGAACAATGACAACTAAGAAAACTAGAATTAAACATTCCCCTGA
ATTTAAAGCAGAAGCCCTGAAGTTAGCAGATAAAGTGGGAGTAGCTGCGGCAGCAAGACAGCTTTCTTTACATGAATCTCAGCTCTACGGGTGGCGAAAG
AACTCGAAAAAAGACACCAGTACTAGTCAGCGAGAGCAAGAACTAGCCGCAGAGGTTGCCAAGCTCAAAAGGCAGTTGGCTGAGCAAGCAGAAGAGCTAG
ACATTGTAAAAAAGGCCGCCACCTACTTCGCGAAGAATCTAAAGTAAATTTCTATGAATTTATGCTCGAACACCTGCTGAGCTTCAGTATTGTGCGTATG
GCTAAGGTGTTCGGGGTATCCCGAAGTGGGTTTTATTACTGGGTTAAGCATCGCCATAAGGCCATCAAACACGAGGCAATTAGCCAAAAGCTTGATACAA
GAGTCAAAGAAGCTTTCGACAACAGTAAAGGCCGAGATGGCTCAAGGCGTATTCAAAAAGAACTGTCTGAGAACGGTGATAACTACAACGTTAAAATCAT
TGCTGCCAGTATGAAGCGTCAAGATTTAACGCCGAAAGCTGCACGCAAGTTTAAGTGCACTACGGACAGCAAGCATCAAATGCCTGTTGCTCCAAATCTG
TTGGCTCAGAACTTTAGCGCATCGGCTCCGAATGAAAAATGGGCTGGAGACATCACCTATATAGCCACAAGCGAAGGCTGGTTGTACTTGGCGGTAATTA
TTGACCTTTACTCAAGACAAGTAATTGGATGGTCTATGGATACCAGAATGACGGCTACGCTGGTCTGTAATGCGCTATCAATGGCATTGTTCCGTCGCGG
ATTCCCCGAGCAGGTTATCGTTCATAGTGATCGAGGTAGTCAGTACTGCTCAAAAGATTATCGAGATCTCATAACTGCTTATAATCTAAAGCAAAGTATG
AGTAGGAAAGGAAACTGCTGGGACAATGCTTGTGTTGAGAGCTTCTTCCATTCTATGAAAGTGGAAGCGATACAATATGAGCCAATTATGACGAGAGACC
AGATGCACCAAACGATCTTCGAGTACATAGAGGTTGATTATAATCGGACGAGAAGGCACAGTGCTCTTGGGTATCTAAGCCCAATTAACTTTGAACAGAA
AAATGTCGCTTAATGAAGTGTCCAGTTTGGCTGGAGCAGATCA
ATTTAAAGCAGAAGCCCTGAAGTTAGCAGATAAAGTGGGAGTAGCTGCGGCAGCAAGACAGCTTTCTTTACATGAATCTCAGCTCTACGGGTGGCGAAAG
AACTCGAAAAAAGACACCAGTACTAGTCAGCGAGAGCAAGAACTAGCCGCAGAGGTTGCCAAGCTCAAAAGGCAGTTGGCTGAGCAAGCAGAAGAGCTAG
ACATTGTAAAAAAGGCCGCCACCTACTTCGCGAAGAATCTAAAGTAAATTTCTATGAATTTATGCTCGAACACCTGCTGAGCTTCAGTATTGTGCGTATG
GCTAAGGTGTTCGGGGTATCCCGAAGTGGGTTTTATTACTGGGTTAAGCATCGCCATAAGGCCATCAAACACGAGGCAATTAGCCAAAAGCTTGATACAA
GAGTCAAAGAAGCTTTCGACAACAGTAAAGGCCGAGATGGCTCAAGGCGTATTCAAAAAGAACTGTCTGAGAACGGTGATAACTACAACGTTAAAATCAT
TGCTGCCAGTATGAAGCGTCAAGATTTAACGCCGAAAGCTGCACGCAAGTTTAAGTGCACTACGGACAGCAAGCATCAAATGCCTGTTGCTCCAAATCTG
TTGGCTCAGAACTTTAGCGCATCGGCTCCGAATGAAAAATGGGCTGGAGACATCACCTATATAGCCACAAGCGAAGGCTGGTTGTACTTGGCGGTAATTA
TTGACCTTTACTCAAGACAAGTAATTGGATGGTCTATGGATACCAGAATGACGGCTACGCTGGTCTGTAATGCGCTATCAATGGCATTGTTCCGTCGCGG
ATTCCCCGAGCAGGTTATCGTTCATAGTGATCGAGGTAGTCAGTACTGCTCAAAAGATTATCGAGATCTCATAACTGCTTATAATCTAAAGCAAAGTATG
AGTAGGAAAGGAAACTGCTGGGACAATGCTTGTGTTGAGAGCTTCTTCCATTCTATGAAAGTGGAAGCGATACAATATGAGCCAATTATGACGAGAGACC
AGATGCACCAAACGATCTTCGAGTACATAGAGGTTGATTATAATCGGACGAGAAGGCACAGTGCTCTTGGGTATCTAAGCCCAATTAACTTTGAACAGAA
AAATGTCGCTTAATGAAGTGTCCAGTTTGGCTGGAGCAGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 63 | 347 | + | No |
Description : First part of the transposase
ORF sequence :
MTTKKTRIKHSPEFKAEALKLADKVGVAAAARQLSLHESQLYGWRKNSKKDTSTSQREQELAAEVAKLKRQLAEQAEELDIVKKAATYFAKNLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 284 | 1213 | + | No |
Description : Second part of the transposase
ORF sequence :
ASRRARHCKKGRHLLREESKVNFYEFMLEHLLSFSIVRMAKVFGVSRSGFYYWVKHRHKAIKHEAISQKLDTRVKEAFDNSKGRDGSRRIQKELSENGDN
YNVKIIAASMKRQDLTPKAARKFKCTTDSKHQMPVAPNLLAQNFSASAPNEKWAGDITYIATSEGWLYLAVIIDLYSRQVIGWSMDTRMTATLVCNALSM
ALFRRGFPEQVIVHSDRGSQYCSKDYRDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTRDQMHQTIFEYIEVDYNRTRRHSALGYLSP
INFEQKNVA
YNVKIIAASMKRQDLTPKAARKFKCTTDSKHQMPVAPNLLAQNFSASAPNEKWAGDITYIATSEGWLYLAVIIDLYSRQVIGWSMDTRMTATLVCNALSM
ALFRRGFPEQVIVHSDRGSQYCSKDYRDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTRDQMHQTIFEYIEVDYNRTRRHSALGYLSP
INFEQKNVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 63 | 1213 | + | Yes |
Chemistry : DDE
ORF sequence :
MTTKKTRIKHSPEFKAEALKLADKVGVAAAARQLSLHESQLYGWRKNSKKDTSTSQREQELAAEVAKLKRQLAEQAEELDIVKKGRHLLREESKVNFYEF
MLEHLLSFSIVRMAKVFGVSRSGFYYWVKHRHKAIKHEAISQKLDTRVKEAFDNSKGRDGSRRIQKELSENGDNYNVKIIAASMKRQDLTPKAARKFKCT
TDSKHQMPVAPNLLAQNFSASAPNEKWAGDITYIATSEGWLYLAVIIDLYSRQVIGWSMDTRMTATLVCNALSMALFRRGFPEQVIVHSDRGSQYCSKDY
RDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTRDQMHQTIFEYIEVDYNRTRRHSALGYLSPINFEQKNVA
MLEHLLSFSIVRMAKVFGVSRSGFYYWVKHRHKAIKHEAISQKLDTRVKEAFDNSKGRDGSRRIQKELSENGDNYNVKIIAASMKRQDLTPKAARKFKCT
TDSKHQMPVAPNLLAQNFSASAPNEKWAGDITYIATSEGWLYLAVIIDLYSRQVIGWSMDTRMTATLVCNALSMALFRRGFPEQVIVHSDRGSQYCSKDY
RDLITAYNLKQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTRDQMHQTIFEYIEVDYNRTRRHSALGYLSPINFEQKNVA
Blast result :
Comments
ISVal2 is 96% aa similar to ISVpa2.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Ren, C., Jiang,X., Sun,H., Luo,P., Chen,C., Zhao,Z. and Hu,C. (2012) Ann. Microbiol. 62 (1), 69-75
2] Ren, C. (2010) Direct submmission
2] Ren, C. (2010) Direct submmission