ISStma16
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Stenotrophomonas maltophilia | Stenotrophomonas maltophilia D457 |
DNA section
IS Length : 846 bp
Ends
IR Length : 16/17
IRL : GAACGTGTTCAAAGTCTCGCCCTCTTGATGTGAGAAGCTCGCTGGGTGAG
IRR : GAGCGTGTTCAAAGTCTTTTGAGCAAGAGCACCAAGAATGCCAAGTGGAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGCAAGATC | TGA | GACGCACTTC | 3 |
| ACGTGCCGCC | TGA | GGGCTACCCG | 3 |
| GTTCAGGCCC | TCA | GGACCGACAC | 3 |
DNA sequence
GAACGTGTTCAAAGTCTCGCCCTCTTGATGTGAGAAGCTCGCTGGGTGAGAAAAGGCTACCCCAGCGACATCAAGCGCGAGCAGTTCGAGGTGATCCGGC
CATTGCTGGAGAGTGCGCGCAGAAAGACTGCCCCACGCAAAGTGGAGCTGTACGAGGTGTTCTGCGCCGTGCTGTACCTGCTGCGCACGGGCTGTCAGTG
GCGGGCACTGCCCAGCGACTTTCCCAAATGGCGCACCGTGCACTCGTACTTTGCCATCTGGAGCGAGCCACGCGAGGGTGGCAGCCTGCTGGAGCAGGCA
TTAAAAAAATCAGGTTGGCGCGGCCCGCGAGAAACTGGGGCGCAACGCCTGCAGCACGCTCTTGATCGTGGACGCGCAGAGCGTGAAGAACACGGACACG
GCGGGCTTGAAGGGCTATGACGCAGGCAAGAAGGTCTCGGGCATCAAGCGCCACATCGCGGTGGACACCCAGGGATTGCCGCATGCGATAGCGGTGACCA
CGGCCGAGGTGACGGACCGCAAAGGCGCACTGCAGGCGCTACAGCGCTGCAAGCCCCGGTTGAGCCGGCTACAAAGCCTGCTGTGCGATGCAGGCTACAT
CGGTCGGCCGTTCGCGCAAGGCGTGCAGCAGATTCTGGGAGGGCATGTGACGGTGCAGATCGCCAAGAGAAGCGAGCTTCACACTTTCAAAGTGATGCCC
AAGCGCTGGGTCGTGGAACGCAGCTTTGCCTGGCTGGACAAGAACAGGAGGCTGTGGAAGAACTGCGAGCGATGGCTCAACACCAGCCTGCAGTTCGTCC
ACTTGGCATTCTTGGTGCTCTTGCTCAAAAGACTTTGAACACGCTC
CATTGCTGGAGAGTGCGCGCAGAAAGACTGCCCCACGCAAAGTGGAGCTGTACGAGGTGTTCTGCGCCGTGCTGTACCTGCTGCGCACGGGCTGTCAGTG
GCGGGCACTGCCCAGCGACTTTCCCAAATGGCGCACCGTGCACTCGTACTTTGCCATCTGGAGCGAGCCACGCGAGGGTGGCAGCCTGCTGGAGCAGGCA
TTAAAAAAATCAGGTTGGCGCGGCCCGCGAGAAACTGGGGCGCAACGCCTGCAGCACGCTCTTGATCGTGGACGCGCAGAGCGTGAAGAACACGGACACG
GCGGGCTTGAAGGGCTATGACGCAGGCAAGAAGGTCTCGGGCATCAAGCGCCACATCGCGGTGGACACCCAGGGATTGCCGCATGCGATAGCGGTGACCA
CGGCCGAGGTGACGGACCGCAAAGGCGCACTGCAGGCGCTACAGCGCTGCAAGCCCCGGTTGAGCCGGCTACAAAGCCTGCTGTGCGATGCAGGCTACAT
CGGTCGGCCGTTCGCGCAAGGCGTGCAGCAGATTCTGGGAGGGCATGTGACGGTGCAGATCGCCAAGAGAAGCGAGCTTCACACTTTCAAAGTGATGCCC
AAGCGCTGGGTCGTGGAACGCAGCTTTGCCTGGCTGGACAAGAACAGGAGGCTGTGGAAGAACTGCGAGCGATGGCTCAACACCAGCCTGCAGTTCGTCC
ACTTGGCATTCTTGGTGCTCTTGCTCAAAAGACTTTGAACACGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 375 bp | 124 aa | 46 | 420 | + | No |
Description : First part of the transposase
ORF sequence :
VRKGYPSDIKREQFEVIRPLLESARRKTAPRKVELYEVFCAVLYLLRTGCQWRALPSDFPKWRTVHSYFAIWSEPREGGSLLEQALKKSGWRGPRETGAQ
RLQHALDRGRAEREEHGHGGLEGL
RLQHALDRGRAEREEHGHGGLEGL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 534 bp | 177 aa | 305 | 838 | + | No |
Description : Second part of the transposase
ORF sequence :
KNQVGAAREKLGRNACSTLLIVDAQSVKNTDTAGLKGYDAGKKVSGIKRHIAVDTQGLPHAIAVTTAEVTDRKGALQALQRCKPRLSRLQSLLCDAGYIG
RPFAQGVQQILGGHVTVQIAKRSELHTFKVMPKRWVVERSFAWLDKNRRLWKNCERWLNTSLQFVHLAFLVLLLKRL
RPFAQGVQQILGGHVTVQIAKRSELHTFKVMPKRWVVERSFAWLDKNRRLWKNCERWLNTSLQFVHLAFLVLLLKRL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 793 bp | 264 aa | 46 | 838 | + | Yes |
Chemistry : DDE
ORF sequence :
VRKGYPSDIKREQFEVIRPLLESARRKTAPRKVELYEVFCAVLYLLRTGCQWRALPSDFPKWRTVHSYFAIWSEPREGGSLLEQALKKNQVGAAREKLGR
NACSTLLIVDAQSVKNTDTAGLKGYDAGKKVSGIKRHIAVDTQGLPHAIAVTTAEVTDRKGALQALQRCKPRLSRLQSLLCDAGYIGRPFAQGVQQILGG
HVTVQIAKRSELHTFKVMPKRWVVERSFAWLDKNRRLWKNCERWLNTSLQFVHLAFLVLLLKRL
NACSTLLIVDAQSVKNTDTAGLKGYDAGKKVSGIKRHIAVDTQGLPHAIAVTTAEVTDRKGALQALQRCKPRLSRLQSLLCDAGYIGRPFAQGVQQILGG
HVTVQIAKRSELHTFKVMPKRWVVERSFAWLDKNRRLWKNCERWLNTSLQFVHLAFLVLLLKRL
Blast result :
Comments
ISStma16 is 89% aa similar to ISAzo37. The third ORf is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2012)
2] Felipe Lira (2012) Direct submission.
2] Felipe Lira (2012) Direct submission.