ISLbi2
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010842 | ND | Leptospira biflexa | Leptospira biflexa serovar Patoc 'Patoc 1 (Paris)' chromosome I Leptospira biflexa serovar Patoc 'Patoc 1 (Ames)' chromosome I |
DNA section
IS Length : 1062 bp
Ends
IR Length : 27/33
IRL : GGTAGGTTCAAGAATCTAATGCAACAAAACCCGATAATGTAAATCGGAGA
IRR : GGTAGGTTCAAGAATCTAGCGCACCAGCGCCCGTATTTCTTCTTCAGGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGGATTAAA | AAA | GTAATTACGC | 3 |
| CACAAATTCC | AAG | TATTTTTTTT | 3 |
| CGGGATTAAA | AAA | GTAATTACGC | 3 |
| CACAAATTCC | AAG | TATTTTTTTT | 3 |
DNA sequence
GGTAGGTTCAAGAATCTAATGCAACAAAACCCGATAATGTAAATCGGAGAAAAAATGGAAAAATACTCTAGAATTAGTCCGAAGGAAAGGGAAACCTTGT
CCGTGTTGATTCATCAAGGATACTCTAAAGGTGCGATTGCAGAAATGATGGGTCGTCACCGATCTACAATTAGTAGAGAATTAGAAAAAGGAAAAATTTC
CGATAGATATTATCAATACTCCGCAATTATTTCTGGGTATAAATCAAGAATGGCTGCCTCATCTAGAAAGCTTGGAAAAAGTAAATTATTAACTAACAAC
AAGTTGTATAGGGAGTTTAAAAATCGTCTTGAACTCAAATGGTCACCAGTACAAATATCAAAAAATCTATCTCGTTCGTTTCCAAATGAAAAATATATGA
ATATTTCTCACCAATCAATTTATACCTTTATCTATTTGCTTCCGAGAGGAGAGCTTAAAAAAGAAATCAATACTTACCTTAGAAGAAAAGGCAAAAAAGC
TAAACCTGCGGGAATCAAGGCCAATATAGGTAAAATTCCAGATATGATCAGCATTGAAGAACGACCAAAGGAAGTCGCTGATAGGTCTGTTCCAGGTCAT
TGGGAAGGCGATTTACTCATAGGAAATAACCATTCAAGTGCTCTTGGTACAATTGTAGAAAGAGTTACAAGAACTGTCATACTAGTACCTTTGAAAGCCA
TGGATGCTGAATCTGTCAGAAAGGCCTTTGAACAAGAGTTAAAACAGTTTCCAAAACAATTAAAACTTTCTATGACTTATGACCAAGGAAAAGAAATGTC
AGAACATAGATTATTTACTAAAAATACAAAAATGAAAGTTTATTTCTGTCATCCAGCTTCTCCATGGGAAAGGGGAACATGTGAAAATACTAATATGCTT
ATCAGAGACTTCTTTCCAAAGGGAACTGACTTTTCAAAACTCAAAAAAAGCCAAATTAAAAAGGTTCAAACTTTACTCAATCAAAGACCTAGAAAAACTT
TGAATTGGAATACACCTGAAGAAGAAATACGGGCGCTGGTGCGCTAGATTCTTGAACCTACC
CCGTGTTGATTCATCAAGGATACTCTAAAGGTGCGATTGCAGAAATGATGGGTCGTCACCGATCTACAATTAGTAGAGAATTAGAAAAAGGAAAAATTTC
CGATAGATATTATCAATACTCCGCAATTATTTCTGGGTATAAATCAAGAATGGCTGCCTCATCTAGAAAGCTTGGAAAAAGTAAATTATTAACTAACAAC
AAGTTGTATAGGGAGTTTAAAAATCGTCTTGAACTCAAATGGTCACCAGTACAAATATCAAAAAATCTATCTCGTTCGTTTCCAAATGAAAAATATATGA
ATATTTCTCACCAATCAATTTATACCTTTATCTATTTGCTTCCGAGAGGAGAGCTTAAAAAAGAAATCAATACTTACCTTAGAAGAAAAGGCAAAAAAGC
TAAACCTGCGGGAATCAAGGCCAATATAGGTAAAATTCCAGATATGATCAGCATTGAAGAACGACCAAAGGAAGTCGCTGATAGGTCTGTTCCAGGTCAT
TGGGAAGGCGATTTACTCATAGGAAATAACCATTCAAGTGCTCTTGGTACAATTGTAGAAAGAGTTACAAGAACTGTCATACTAGTACCTTTGAAAGCCA
TGGATGCTGAATCTGTCAGAAAGGCCTTTGAACAAGAGTTAAAACAGTTTCCAAAACAATTAAAACTTTCTATGACTTATGACCAAGGAAAAGAAATGTC
AGAACATAGATTATTTACTAAAAATACAAAAATGAAAGTTTATTTCTGTCATCCAGCTTCTCCATGGGAAAGGGGAACATGTGAAAATACTAATATGCTT
ATCAGAGACTTCTTTCCAAAGGGAACTGACTTTTCAAAACTCAAAAAAAGCCAAATTAAAAAGGTTCAAACTTTACTCAATCAAAGACCTAGAAAAACTT
TGAATTGGAATACACCTGAAGAAGAAATACGGGCGCTGGTGCGCTAGATTCTTGAACCTACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 993 bp | 330 aa | 55 | 1047 | + | No |
Chemistry : DDE
ORF sequence :
MEKYSRISPKERETLSVLIHQGYSKGAIAEMMGRHRSTISRELEKGKISDRYYQYSAIISGYKSRMAASSRKLGKSKLLTNNKLYREFKNRLELKWSPVQ
ISKNLSRSFPNEKYMNISHQSIYTFIYLLPRGELKKEINTYLRRKGKKAKPAGIKANIGKIPDMISIEERPKEVADRSVPGHWEGDLLIGNNHSSALGTI
VERVTRTVILVPLKAMDAESVRKAFEQELKQFPKQLKLSMTYDQGKEMSEHRLFTKNTKMKVYFCHPASPWERGTCENTNMLIRDFFPKGTDFSKLKKSQ
IKKVQTLLNQRPRKTLNWNTPEEEIRALVR
ISKNLSRSFPNEKYMNISHQSIYTFIYLLPRGELKKEINTYLRRKGKKAKPAGIKANIGKIPDMISIEERPKEVADRSVPGHWEGDLLIGNNHSSALGTI
VERVTRTVILVPLKAMDAESVRKAFEQELKQFPKQLKLSMTYDQGKEMSEHRLFTKNTKMKVYFCHPASPWERGTCENTNMLIRDFFPKGTDFSKLKKSQ
IKKVQTLLNQRPRKTLNWNTPEEEIRALVR
Blast result :
Comments
ISLbi2 is 61% aa similar to ISLxc3.
References
1] ISfinder annotation (2012)
2] Picardeau,M., Bulach,D.M., Bouchier,C., Zuerner,R.L., Zidane,N., Wilson,P.J., Creno,S., Kuczek,E.S., Bommezzadri,S., Davis,J.C., McGrath,A., Johnson,M.J., Boursaux-Eude,C., Seemann,T., Rouy,Z., Coppel,R.L., Rood,J.I., Lajus,A., Davies,J.K., Medigue,C. and Adler,B. (2008) PLoS ONE 3 (2), E1607.
2] Picardeau,M., Bulach,D.M., Bouchier,C., Zuerner,R.L., Zidane,N., Wilson,P.J., Creno,S., Kuczek,E.S., Bommezzadri,S., Davis,J.C., McGrath,A., Johnson,M.J., Boursaux-Eude,C., Seemann,T., Rouy,Z., Coppel,R.L., Rood,J.I., Lajus,A., Davies,J.K., Medigue,C. and Adler,B. (2008) PLoS ONE 3 (2), E1607.