ISfinder Mirror Site

ISSoEn4

Family ISL3
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISsope4

Accession number	Transposition	Origin	Host
AM921792	ND	Primary endosymbiont	Primary endosymbiont of Sitophilus oryzae

DNA section

IS Length : 1712 bp

Ends

IR Length : 24

IRL : GGGTCTTCCCCTGTTGTGGTGGCTAAGGGCATTATGATGGCAGTCTTGAT
IRR : GGGTCTTCCCCTGTTGTGGTGGCTCAATCGACAGTTCTGCATTTTTACTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAAACACTGG	TCATTTAT	ACAGACTTAA	8
GAAAGACTGT	ATAAACAA	ACAGGGGACG	8
GCCAAAGTGT	ACATTTGT	ACATTATCAT	8
TTTGTCCTGT	AGATTGAA	ACAGACATTG	8
TTTGTCCTGT	AGATTGAA	ACAGACATTG	8
CTGTATATAT	AT	GCATGTACAG	2
ATAAGCATGG	AAATAAAA	ACACCGCCAC	8
GTTGAATTAT		TGCTTTGTTGA	0
TGATTACTGG	TATTGTAT	CCAGTAGTTAT	8
TTGTTATTAT	AT	AAATAAACAGT	2
GTACAGTCAT		ACAGTTCATAC	0
TCAAGGCTGT	AAATAGAA	ACAGTACTCGT	8
GTGTTACTGT	ATAAAAAC	ACAGTATCCGG	8
GTCTGCATTA		CTGTATTATCA	0

DNA sequence

GGGTCTTCCCCTGTTGTGGTGGCTAAGGGCATTATGATGGCAGTCTTGATTTTTGAGGAGTCTGCCATGGACGAAAAGTCCCTCTATGCCCATATCCTTA
ACCTGTCCGCACCGTGGCAGGTACAATCCCTTTCTCTTAATGAAAAATCTGGATCAGTGACGGTAATTGTCGGCATTGCCGAGCACACACAACTGGCCTG
CCCAACCTGCGGTAAATCCTGCTCCATACATGATCACCGGCGTCGTAAATGGCGTCACCTCGATACCTGTCAGTTCACCACGCTGGTTGAGGCTGATGTC
CCCCGCGTTGACTGCCCCGAGCACGGTTGCCAGACACTGCCTGTTCCCTGGGCAGGGTCAGGCAGCCGCTACACCTTGTTGTTCGAAGCCTTTGTTCTTT
CATGGCTGAAAGTCAGCACCGTGGATGCTGTCAGAAAGCAGCTCAAACTCAGTTGGAATGCCGTTGAAGGCATCATGATGCGCGCAGTCAAACGAGGCTT
GGCCCGGATAAAACAACCCTTATCCGCCCGTTACCTCTGCGTGGATGAAGTCGGGTTCAAAAAAGGACACCAGTACGTCACCGTTATCTCTGACAGGCAG
GGACGCGCTTTGCAACTGACCGACGACCGCGGTGTAGAAAGCCTTGCCAGTTATCTGCGCAGCCTGAGAGATCACCAGCTTGATGAGATAAAAACGCTGT
CTATGGACATGAACATGGCCTATATCAGTGCAGCCCGCATCCATCTTCCCAATGCCGTCGATAAAATTGCTTTCGATCACTTCCATGTGGCAAAAATGTT
GTGCGCCGTCGTTGATAAAACCCGTCAGGGTGAGATGAAACAGATCCCGTCGTCAGACAGGAAAGACGCCCACCGCTCACGCTATCTATGGTTTTACAGC
AAACAAAATCGCCTCGGGTGCTGGGCAGAGAGGTTAGAAGTTGCCCGGCTGATGTTACCCCAAACGAGCCAGTGCTGGGTAATGAAAGAGCTTGCTCGCG
ATCTGTGGCACCGCCGCTATGACAATCATAGCCGTAAGCTGTGGCAGGAATGGATGGCGATGGCTAAAGACACCGGCATACCGCTCATGGCCAGCATTGC
CCGCATGGTGGCAAAACGCCTTTACGGCATTCTGAATGCAATGAAAAACCGGGTATCAAATGGGAATGCGGAGTCCCTGAACAGCAAAATACGGCTGTTC
AGGATCAAGTCACAGGGCTTCAGGAACAAAGAACGTTTCAAGCTGGGCGTAATGTTCCACTATGGGAAACTAAATATGAATTTTTGAGCAATCATTTAAG
AGAGTTATAAAATTTTTTTTGCTGGTGATGTTGTATTCGCTCGTCATTCTCCTTTTCTTTTTCCTTTTGCCACATATTACTGCATCCCGGATCGCTAGGG
GTAAGACAATATTGAGTTTTATCAACTTGAGATTCACTTTTCTCCCAAGATAATTTTGGCGTTACCTCTGGTGACAGTGAATAGTCTTTCTTATCAGTGT
CATACATCATATTTGGTGAGGTCGATGCTGCAACACCATGAAGCAGTAAGATAAATGGGGTATATGCATATTTTTTTAAGCATGACATGAACGTTATTCC
TTTAAACGTAAATATGTTTGACATCCATACAGAAGTCCAAGTTCCAGCCAAAAGAATAAGTAAAGTAAAAATGCAGAACTGTCGATTGAGCCACCACAAC
AGGGGAAGACCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1221 bp	406 aa	67	1287	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MDEKSLYAHILNLSAPWQVQSLSLNEKSGSVTVIVGIAEHTQLACPTCGKSCSIHDHRRRKWRHLDTCQFTTLVEADVPRVDCPEHGCQTLPVPWAGSGS
RYTLLFEAFVLSWLKVSTVDAVRKQLKLSWNAVEGIMMRAVKRGLARIKQPLSARYLCVDEVGFKKGHQYVTVISDRQGRALQLTDDRGVESLASYLRSL
RDHQLDEIKTLSMDMNMAYISAARIHLPNAVDKIAFDHFHVAKMLCAVVDKTRQGEMKQIPSSDRKDAHRSRYLWFYSKQNRLGCWAERLEVARLMLPQT
SQCWVMKELARDLWHRRYDNHSRKLWQEWMAMAKDTGIPLMASIARMVAKRLYGILNAMKNRVSNGNAESLNSKIRLFRIKSQGFRNKERFKLGVMFHYG
KLNMNF

Blast result :

Comments

ISSoEn4 is 65% aa similar to ISShes3. There 14 complete copies in the genome.

References

1] ISfinder submission (2011)
2] Kelly Oakeson (2011) Direct submission
3] Gil,R., Belda,E., Gosalbes,M.J., Delaye,L., Vallier,A., Vincent-Monegat,C., Heddi,A., Silva,F.J., Moya,A. and Latorre,A.(2008) Int. Microbiol. 11 (1), 41-48.