ISfinder Mirror Site

ISSoEn3

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISsope3

Accession number	Transposition	Origin	Host
AM921791	ND	Primary endosymbiont	Primary endosymbiont of Sitophilus oryzae

DNA section

IS Length : 2600 bp

Ends

IR Length : 33/48

IRL : TGCTGGTTCCGGTCACATCCGATCACTGATTCCGATTTCACCCGATCACT
IRR : TGCAGATTCCGACGTATCCGATCAGCTGTTCCGGCGACATCCGATCAGTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTCCCGTTCA	CCCAT	CATCTCGCCC	5
TTTACGCCCG	CCACG	GCGGTAAAAT	5
CCGCTACAAT		ACATCAAGGC	0
GCCTTTCGTT	GCCCG	CAGGGCGTTA	5
CTTTGCCATG		AACAGCCGGC	0
GCTGTGGTGG		CTGCCCGCGT	0
GAGGCAGTGT		ATACTCGCGT	0
GTCCGGCAGG	ACAGC	CGCATCATCA	5
TGCCTGAGCG	GTACT	GGCGGCCAGG	5
TTCTAACCTA	CCTAC	CCCTAACTAC	5
TTCATTTTCG	GCCAAC	CACATACCTA	6
GTCGTCCGCC	GCTTT	TCTGCGCGGG	5
GCGTTACTGG		CACGGCACCT	0
TGCGGCTGGA	CCGGT	GGCACTGCAC	5
TTTGATATGT		GAGCCTGTTT	0
CTTCGATGTT	GATGC	CCAGCGCCAG	5
TTCTAAACAG		TAATCAGATC	0
GGGGATTACG	GGGGGGGGGG	CGCGCTTCGC	10
GGTGACTGCG	CCAGC	CACGACAGCA	5
GCCCAGCGCC	AGGAA	CACAGATTTG	5
ATCCGCCTGT		GAGGCCTGTT	0

DNA sequence

TGCTGGTTCCGGTCACATCCGATCACTGATTCCGATTTCACCCGATCACTAATTCTGATTTCATCCGATCACTGATTCCGGTCGCCCGATCAGCGATTCC
GATTCTGTCCGATCGCTCATCTTCTGTTCCGCCATACTCTGGAGACTTTTAGCTTCCGGGGGCATGGCATGGCACGTAAAAAAAAGAAAGCGAGAACGGA
AATGTGCATCTATATTAATGTCTTACGTATGAAATTCGAGCAGCGTCGCTCGAATCGCACTATCGCAGCAGCGCTCGGCATAGGCTGTACTACCGTGCAC
GATATCCTCGGCCGATTCACGGTAGTTAACCTGGTCTGGCCATTGCCGGCGGAACTGTCCCCCGTCGACCTCGACCGCCTGCTCTATCCCGGCAAATCCG
GAAAAGTTATCAATACATTACCCAGCTGGCTTGATATCGATACCGAGTTAAGCCGCAAGGGCATGACCAAGCAGCTGCTCTGGATGGAATATCAGTCCGC
CGTGGGCGGTGATGCCCTCGGTTACTCACAGTTTTGTGCACTGTTCCGTGACTGGAAAAAGAAGCAGAGGCGTTCTATGCGCATGGAGCACAAGGCTGGC
GAAAAGCTCTTCATCAACTTCTGTGGCCCCACCGTACCTATCGTCAACCCTGCGACCAGTAGCGTACGCCAGGTCGCTATCTTCGTCGCTGCCATGGGCG
TGTCAGGCTATGCGTATATCGAAGCCTGCGAAAGCCAGGACATGGCATCGTGGCTCAACGCCAATAGCCGCTGTCTGCACTTCATGGGTGGGGTTCCGGA
GCTGATGATACCTGATAATCTGCGCAGCGCTGTCAGCACCCCTGACCGCTATGAGCCGGTCATAAACCAGAGCTACCAGGCGCTGGCAAATCACTATGAG
ACAGTGGTGCTACCGGCGCGCCCGAGAAAACCGAAAGACAAGGCGAAGGCAGAATCAACTGTGCAGCTGGTAGAACGCTGGGTTTTGGCCCGGTTGCGTA
AACGTAGGTTCTACTCGCTGCCCGAACTCAACCAGGTGATACGAGAACTCAATCATGAGTTGAATCTGCGCCCGATGCGTCATTACGGCGGACAAAGTCG
CCTTGAACGCTTCGAGCAGCTGGACAAACCGGCTCTTGGGCCTCTACCGCCCACACAATGGGAATACAGTGAGTATCTCGTTGCCCGAGTGGGACCTGAT
TACCACATAGACTACGGCAAAAACTGGTACTCGGTGCCGCATCCGCTGGTTGGCGAGCGCGTTGACGTCATCGCCACCCAACGGCTGGTGCAAATCCACC
ATAAGGGCGTCTGCGTGGCTACGCACCCTCGCAGCGATAACGCCTATAGGCACACGACTCAGGCGGCGCACATGCCGGCTAACCATAAGGGGCAGAGTCA
GTGGACGCCGGAAAGGCTGTGCAGTTGGGCGCTGTCGGTGGGTGTGTGCACACTGAAAGTGGTCGAGTCCATCCAAAAGAGCAAAGCCCATCCGGAGCAG
GCTTACCGCTCCGTGCTGGGGCTACTCAATCTGCAACGGCGCTATGAGACGACGCGACTGGAGAAGGCCTGCGTGCTGGCGTTGGAGAAAGGGTGCATTA
ACCGCTCTTTCATAGCCAACGTATTGAAACACGGTCGTGAAAGTGAGGTCACCCAGGACGGAGCCGGCGTATCAATGCTGGTTCACGAAAACCTCCGAGG
TCCGGACAGTTATCACTAAGGAGAATAAATATGGATACACTGTTAATGGCTCTGCGAGAGCTGAAGTTGTCGGCAATGGTCCAGGCGTTGGAGACGCAAC
GCGAACTCCCGGGGAGTTATGGGGAGCTGGGGTTCGAGGAGCGGTTGTCGCTGATGGTAGAAGCGGAAAATTTACATAGAAAAAACAACCACATATACCG
TATTCGACGGCAATCGCAAATGCGCTTGCAGGCAAAACCGGAAGATATCCGCTATATCCCTAGCCGAGGAGTGACACCGGAACAGATGCGAGATCTGCTA
GGGGGACAATATCTGAAATATCAGAAAAGCATACTCATCACGGGGCCGACAGGTACGGGCAAAACCTGGCTCAGTTGTGCGCTTAGTGAGCAGGCATGCC
GGCAGCAATATAGCGTGCGTTACTGGCGAGTGGGTCGGTTGCTGGCCCATCTTCACCAGTGTCAGGTAGACGGGACCTATCTAAAACAGCTTAAGCAGTT
AGAAAAAATAGAGTTACTGATCTTGGACGATGTGGGCCTAGAATCAATAAGTCCGATGCAGGCAACGATGCTGTTGGAGGTGATGGAAGATCGCTACGAC
AAAAGCAGCAGCATCCTGATCAGTCAACTGCCGGTGAAAAAATGGTATGGACTGATAGAAAACCCCACGACAGCTGACGCGTTACTCGATCGGTCAGTAC
ACCCCAGCTATAGACTGGAACTTAAAGGCGAATCACTACGCAAAGAGCAAGGAGTAGCCAGCACAGGAAAAATAGACTAAACCCGAGTCAGAAGATGAGC
GAACACGTGATCGAATATCACTGGAATGGGTGATCGGAAAATATCGGAATAACTGATCGGATGTCGCCGGAACAGCTGATCGGATACGTCGGAATCTGCA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1551 bp	516 aa	169	1719	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARKKKKARTEMCIYINVLRMKFEQRRSNRTIAAALGIGCTTVHDILGRFTVVNLVWPLPAELSPVDLDRLLYPGKSGKVINTLPSWLDIDTELSRKGMT
KQLLWMEYQSAVGGDALGYSQFCALFRDWKKKQRRSMRMEHKAGEKLFINFCGPTVPIVNPATSSVRQVAIFVAAMGVSGYAYIEACESQDMASWLNANS
RCLHFMGGVPELMIPDNLRSAVSTPDRYEPVINQSYQALANHYETVVLPARPRKPKDKAKAESTVQLVERWVLARLRKRRFYSLPELNQVIRELNHELNL
RPMRHYGGQSRLERFEQLDKPALGPLPPTQWEYSEYLVARVGPDYHIDYGKNWYSVPHPLVGERVDVIATQRLVQIHHKGVCVATHPRSDNAYRHTTQAA
HMPANHKGQSQWTPERLCSWALSVGVCTLKVVESIQKSKAHPEQAYRSVLGLLNLQRRYETTRLEKACVLALEKGCINRSFIANVLKHGRESEVTQDGAG
VSMLVHENLRGPDSYH

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
750 bp	249 aa	1731	2480	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MDTLLMALRELKLSAMVQALETQRELPGSYGELGFEERLSLMVEAENLHRKNNHIYRIRRQSQMRLQAKPEDIRYIPSRGVTPEQMRDLLGGQYLKYQKS
ILITGPTGTGKTWLSCALSEQACRQQYSVRYWRVGRLLAHLHQCQVDGTYLKQLKQLEKIELLILDDVGLESISPMQATMLLEVMEDRYDKSSSILISQL
PVKKWYGLIENPTTADALLDRSVHPSYRLELKGESLRKEQGVASTGKID

Blast result :

Comments

There are 45 complete copies in the genome.
ISSoEn3 is 70%(ORFA) and 77%(ORFB) aa similar to ISSso4.

References

1] ISfinder submission (2011)
2] Kelly Oakeson (2011) Direct submission
3] Gil,R., Belda,E., Gosalbes,M.J., Delaye,L., Vallier,A., Vincent-Monegat,C., Heddi,A., Silva,F.J., Moya,A. and Latorre,A.(2008) Int. Microbiol. 11 (1), 41-48.