ISPa40

  • Family Tn3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) TnPa40
Accession numberTranspositionOriginHost
ND Pseudomonas aeruginosa
Pseudomonas aeruginosa DK2
DNA section
IS Length : 6592 bp

Ends


IR Length : 35/38

IRL : TGGGTGCCCGCAGAATTCGGAAAAAATCGTACGCTAAGCCGCGCCATGTG
IRR : GGGGAGCCCGCAGAACTCGGAAAAAATCGTACGCTAAGGTTTTCCGAGCT

Insertion site


Left flankDirect repeatRight flankDR Length
GCGCCTTGTTAGGTAGCGAT0

DNA sequence

TGGGTGCCCGCAGAATTCGGAAAAAATCGTACGCTAAGCCGCGCCATGTGCTTGCGCGCGAGCGCTCTCAAGAGGATGGTCCCTCGTACTTCCGAAAAGC
GCGCCAAAGCACAACGTCATAGACGACCTTGAGTAGGCCGCAGGCCACCAGCGGCGCCGCCAGCCAGCCGGCGGCGAATAGGGCGCCGCCAATGGCGGGG
CTCGCTATGGCATTGAGGCGCTTTACCGCACCGCCGTAGTTGTTTTCCCAGTGGCTGACGATCAGCTTCTCGGACAGGCCGGTGAGTTTGGCCGGATCGC
ATGAAAGAGGTTTCAGTTCGTAAGGCATCTTGGCTTCCTCAGCAAGGTGGATCGGAAACGGCGCGACGAAGGCTGACTCGATCAGGTTCAGCCTTGGTTC
GGAAATACCAGCCACCCCGCCAATCCCGCCGCGAGGATCACGACGACGACGTTGAGCTTGTTCTTCCAGGCATAAAGGAATGCCAAGCCTGCGGCGAAAA
TCGACATGCCCACCGTCAACGATGGGACACGTTCAGCAGTGACCTGCGCCAAGTCGATGGTGGTTGCGCCGATCAGGCCGACTACCCCAGCCGCGACGCC
GTCCAGAAAGGCGTGCAGCCGTTTGTTCTCCACGACCGCCTCCAGCCGGTCGTAGAAAATCAGCGAGAACGCAAAGGCCGGAAGAAAGACACCCACCGTC
ATGGCCACCGCCCCGATCGGCCCCCCCGCCACATAGCCGACGAACGTGGCGAAGATGATGAGGGGTGCCGGTAGCACACCGGACAGCGCCAGGCCGTCCA
GGAACTGCCCATCCGTCATCCATCCGCGCCCGACGGCGTCGTTGCGAACGAACGGAATCGCTGTGTAGGCGCCGCCGAAGGTGAGCAAGCCGGCCTTCAG
GCCCGACGCGAAGATGAGCAGCACCGAGGCCTGGCCCTGAACGACCGCTTCCACAAGCTTCGCTGTCGGTGCAGCCCACAAAGCCACGGCCGCGGCCAGC
GCCACCGCCGCCAGTGTCACCAAGAGCGCTGAGGCCCGATGATTCAGCACGAGCAGGGCGTACACAAGGCCGCCCGCCGGCAGGGTGATCCAGAAGTCGA
CACGACCTATGGCTGCCAGCGCGCAAACGATGGCAATGACCCACAACCAGCGATCAAGCAGGATGTGCTCGCCGATACGGTGCACGGCGCGCACGATCAG
GGCGATCACAGCCGCCTGCACGCCAAGGAACGCCGCGCCCAGCGCGGTACCCACGAATTCAATCTGGAAGTACAACCAGGACAGCGCGAACATCAGCAAG
AATCCGGGAAGCATGAAACCGAGTCCCGCCAGCACGCCCCCCAGCCGCCCCTTTGCTCGGATGCCCAAATGAACGCATATTTCATGCGCTTCGGGTCCGG
GCAGCACCTGCATCACCGCAAGCAGCTTGTTGAAGCGTTTGCTGGAGATCCAGCGTTCCTCGTCCACGAGCTCGCGGCGCAACATGTCGATCTGAGCCAC
AGGCCCGCCCCATGCGAGCAAGCCGAACTTGAGGAAGCGCGCGAAGAGCTGTGGGTAGCTCATGGGTGGAGGGACGGCTTCAGTCGTGGCATCCGTCGCC
TTGCTCGGGCGATACGACTCTTCATTCGCAACGCTCATAGATCTTCTCCTTGTAGTTCAACAGTAACCAGTCAAATACGGAAGTTGGCCAACAAGCCGTC
GAATACGGCCGAAGCCTGCGGCCAGCAGCAGGTCGTCTTGGGTGATGTCCCAGAAAGACGGACGGGCCGGGCGTGGTTGCGCGGCGGTCTGAGGACTGGT
CGGGGCGTTCACGTTGTTTTGTCCTCGTAACTCTTCAATAGCCAATCAAAAATTTCGCACGCGCGGGCAAGCAGCGCGTCGTCATCGGGTTCCGATGCGC
GCAAGCCGGCGAGCAGCGATTCGATGCCCAGCGCTTCTGCTACCGGCAGCCCGCCCAAATCCAGGCAATGCACGAGCTTGCCCAGGCGTACCAGTGCAGG
GGCGGAATCAAGTCCGAAACTCGCCAGCAGCGTCTCGAAGGTGACCTTGGTGCCCACATGACTGAAGGCGGCGCCGTCGAAATCGAAGCCGAGCCAGCCA
TTGCGGCAGTCGGAGGGACTCGCCAGCCAGACGATGCGCGCGCTCGGGTCGATGAACCGCCGGATCAGCCAGGCCGAAGCGAGTCGGTCCACCCACGGGC
GCGCCCGCGTGGCCCAGGTCCGACCCTGATAGTCGGCGCGGTTCAGCCGCGGAATATCCGTCTGCCGGGCGGTCGGCTCGTCGGGAGACATACGGCGGGT
GATCGCGTCGCGCAGGTCGTCCAGCAGGCTCAAGGTCTGCCGCTGCGCCTCGCCGGGGAAGAAGTCGATGCGGTTCACCTGCTCGAAGCGGCGCACGAGC
GGCTGGAGCTTGCGCATGGCATCGGCGCCGTCCGGGGATGCCAGGTTACGCCCGAGCGCTTTGATCTCCTCGGCGATGCTGGCGTACTCCTCGCCCCGGT
CGAACAGGGCGCGTAGCGCGGCCTTCTGAGCTTCGTCACACCCCGACAGTCGATAGACCTGGCCGCTGCCCCCGACTTCCACCGCCTGCGCCGCCACTTC
GCCCAGCGTCGCCGCACTGTCCGCCGAGTCGGGCAGCAGATAGACCCCGTCGCGCAGCGTTGCGCAGCCCTGCGCTTTGACAGAACGCCAGACGCGCATG
CGGCCGGTCGATGCCTTGGTGGGCAGGCTGACGAATAGGGCAAGAAAGCTCAAGCACGGGCTCCTTCGTCAAAAAGATGATTAATGTTACAAATACTACA
ATAAGTTATATCTAATACACAACTCATGTCGGAGCGTCAATTTAAGGCATACCCTAACTGGATGTCAGGAATGGCCACGCTGCGCCGTCAGAATAGAGTC
TGCTTTCACATTCTTTGACACATGCTTGCCAAGGTCATAGATTCCAACCTGACAAATTCAAGGCTTAGGGCGCAATGGAACCAAAAACCAACGTAAGCCC
TACCGCCCATCCAGCCTATGGAGGCATCTTGCAGGGACAACGCATCGGTTACGTTCGGGTCAGCAGTTACGACCAGAACCCGGAACGCCAACTTGAGCAG
GTCGAGATCGGCAAGCTGTTCACCGACAAGGCCTCGGGCAAGGACACCCAGCGCCCGCAGCTGGAAGCCATGCTCGGATTCGTCCGCGAAGGCGATACCG
TGGTGGTGCACAGCATGGATCGCCTGGCGCGCAACCTCGACGATCTGCGCCGTCTGGTGCAAAGGCTGACCAAGCGCGGCGTGCGCATCGAGTTCTTGAA
GGAGGGGTTGGTGTTCACCGGCGAGGACTCGCCCATGGCCAACCTCATGTTGTCGGTCATGGGGGCCTTTGCCGAGTTCGAGCGCGCCCTGATCCGCGAG
CGGCAGCGCGAGGGCATCGCCCTGGCCAAGCAGCGCGGTGCTTACCGGGGCCGCAAGAAAGCTCTCTCCGACGATCAAGCCATCACGTTGCGGCAGCGGG
CCGCTGCCGGCGAGCCGAAGGCTCAGCTCGCACGCGAGTTCGGCATCAGCCGTGAAACCCTCTACCAGTACCTTCGCACGGACGACTGAACCATGCCGCG
TCGCTCGATTCTCTCGGCTACGGAGCGCGACACCCTGCTTGCGCTGCCAGAAAGCCAGGATGACCTGATCCGCTACTACACCTTCAACGACTCCGACCTA
TCCCTGATCCGTCAACGGCGGGGCGATGGCAACCGCCTCGGCTTCGCCGTGCAGCTCTGCCTGCTGCGCTACCCTGGTTATGCGCTGGGCAACGACACGG
CGCTACCCGAGCCCGTGATCCAGTGGGTGGCCAAGCAAGTCCAAGCCGACCCCGCGAGTTGGGCGAAGTACGGTGAGCGCGACGTGACCCGCCGCGAGCA
CGCTCTGGAACTGCGCACCTACCTGCAACTGGCCCCGTTCGGTCTGTCGGACTTCCGCGCCCTGGTGCGCGAGTTGACCGAGCTGGCCCAGCAGACCGAC
AAGGGCTTGCTGCTGGCCGGGCAGGCGCTGGAGAGCTTGCGCCAGAAGCGGCGCATCCTGCCGACGCTGAGCGTTATTGACCGGGCGTGTTCGGAGGCCA
TTGCACGCGCCAATCGGCGGGTCTATCGCGCCCTGATCGAGCCGTTGAACCCATCGCATCACGCCAAGCTCGACGAACTGCTCACCATTAAGGCCGGCAG
CAACAGCACTTGGTTGACCTGGTTGCGGCAATCACCGCTGAAGCCGAACTCGCGGCACATGATCGAGCACATCGAGCGGCTGAAGATTTTCCAACTGGTG
GGCCTTCCCGACAGCCTCGGCCGGCACATCCACCAGAACCGATTGCTGAAGCTCGCCCGCGAGGGCGGGCAGATGACGCCACAAGACCTCGGCAAGTTCG
AACCGGAGAGGCGCTATGCCACGCTGGTCGCCGTGGTACTGGAAAGCACCGCGACCGTGATCGATGAACTGGTTGATCTGCACGACCGCATCCTGGTCAA
GCTGTTCAGTGGCGCGAAACACAAGCATCAGCAGCAGTTCCAGAAGCAGGGCAAAGCGATCAATGACAAGGTGCGCCTGTACTCGAAGATCGGCCAGGCC
CTGCTGGACGCTAAAGAGTCCGGTGACGATCCGTTCGCCGCCATCGAGGCGGTCATTCCCTGGGACGAGTTCACCCAGAGTGTCACTGAGGCCGAGCTGC
TGGCCCGGCCGGAAGCCTTCGACCACCTGCACCTGGTCAGCGAGAACTTCGCTACGCTACGCCGCTACACCCCAGCCTTTCTGGAGGTGCTTCAGCTCCG
AGCCGCGACGGCAGCGCAAGCCGTGCTGGATGCCGTTCAGACCCTACGCGAGATGAATGCCGATAATCTGCGCAAGGTGCCCTCCGACGCACCGACCGCC
TTCATCAAGCCGCGCTGGAAACCGCTGGTGATCACCCCGGAAGGCATCGACCGGCGCTTCTACGAAATCTGCGCCTTGTCCGAGTTGAAGAACGCGCTGC
GCTCCGGCGACATCTGGGTCAAAGGCTCGCGGCAGTTCCGCGACTTCGATGACTACCTACTGCCTCCCGAGAAATTCGCCGCACTCAAGCGGGAACAGGC
GCTGCCACTCGCGATCAACCCGAACAGCGACCAGTACCTGGAAGAGCGTTTGCAGCTGCTGGACGAGCAGCTGGCCACTGTCACCCGACTGGCAAAGGAC
AACGAACTACCCGATGCCATCCTCACCGAGTCCGGGCTAAAAATCACCCCGCTGGATTCCGCGGTGCCCAACACCGCGCAGGCGCTGATCGACCAGACCA
GCCAGCTACTGCCGCGCATCAAGATCACCGAGCTGCTGATGGATGTGGATGAATGGACGGGCTTCACCCGCCACTTCACGCACCTGAAGGATGGCGCACA
GGCGAAAGACCGGACGCTACTGCTAACGGCGATCCTGGGCGATGCCATCAACCTCGGCCTGACCAAAATGGCCGAGTCGAGTCCGGGCATGACCTACGCC
AAGCTGTCCTGGTTGCAAGCCTGGCACATCCGTGACGAAACCTACTCGACGGCCCTGGCCGAGCTGGTCAACAGCCAGTTCCGGCATGCCTTCGCCGCCA
ACTGGGGTGACGGCACCACCTCATCCTCCGATGGCCAGCGTTTCCGTGCCGGCGGTAAAGGCGAGAGCACCGGCCACGTCAACCCGAAGTACGGTAGCGA
GCCAGGACGGCTGTTCTACACCCACATCTCTGACCAGTACGCGCCGTTCAGCACCCGCGTGGTGAATGTAGGCGTGCGTGATTCCACCTACGTGCTCGAC
GGTCTGCTGTACCACGAGTCCGATCTGCGGATCGAGGAGCACTACACCGACACCGCTGGCTTCACCGATCACGTCTTCGCCCTGATGCACCTGCTGGGCT
TCCGCTTCGCGCCGCGCATCCGCGACCTCGGTGAAACCAAGTTGTATGTGCCCAATAGCGTCCAAGACTACCCGACGCTGCGTCCAATGCTCGGCGGAAC
CCTGAACATCAAGCACGTCTGCGCTCACTGGGACGAGATTCTGCGGCTGGCCGCGTCGATCAAGCAGGGCACCGTCACCGCATCGCTGATGCTGCGCAAG
CTTGGCAGCTACCCGCGCCAGAACGGCCTGGCCGTGGCTCTGCGCGAGCTGGGTCGGATCGAGCGCACGCTGTTCATCCTCGATTGGCTGCAAAGCGTGG
AACTGCGTCGCCGCGTGCATGCCGGATTGAACAAGGGCGAGGCGCGTAACTCCCTGGCCAGGGCGGTGTTCTTCAACCGCCTCGGCGAGATCAGGGATCG
GAGTTTCGAGCAGCAGCGCTACCGGGCCAGCGGCCTCAACCTGGTGACGGCCGCCATCGTGTTGTGGAACACCGTGTACTTGGAGCGCGCGACCCAGGCC
CGAGAAGAGGCAGGCAAGCCAGTTAATCCTGAGTTACTGCAATACCTTTCGCCATTGGGCTGGGAGCACATCAACCTGACCGGCGATTACGTTTGGCGGC
AAAGCCGCAAGCTGGAAGACGGGAAATTCAGGCCGCTGCGGCAGCTCGGAAAACCTTAGCGTACGATTTTTTCCGAGTTCTGCGGGCTCCCC
Protein section
ORF number : 5

 

ORF 1
LengthBeginEndStrandFusion ORF
261 bp86 aa32868-No
ORF function : Passenger Gene
Annotation : manganese and iron superoxide dismutaseDescription :

ORF sequence :

MPYELKPLSCDPAKLTGLSEKLIVSHWENNYGGAVKRLNAIASPAIGGALFAAGWLAAPLVACGLLKVVYDVVLWRAFRKYEGPSS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1251 bp416 aa1638388-No
ORF function : Passenger Gene
Annotation : chromate resistance proteinDescription :

ORF sequence :

MSVANEESYRPSKATDATTEAVPPPMSYPQLFARFLKFGLLAWGGPVAQIDMLRRELVDEERWISSKRFNKLLAVMQVLPGPEAHEICVHLGIRAKGRLG
GVLAGLGFMLPGFLLMFALSWLYFQIEFVGTALGAAFLGVQAAVIALIVRAVHRIGEHILLDRWLWVIAIVCALAAIGRVDFWITLPAGGLVYALLVLNH
RASALLVTLAAVALAAAVALWAAPTAKLVEAVVQGQASVLLIFASGLKAGLLTFGGAYTAIPFVRNDAVGRGWMTDGQFLDGLALSGVLPAPLIIFATFV
GYVAGGPIGAVAMTVGVFLPAFAFSLIFYDRLEAVVENKRLHAFLDGVAAGVVGLIGATTIDLAQVTAERVPSLTVGMSIFAAGLAFLYAWKNKLNVVVV
ILAAGLAGWLVFPNQG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
945 bp314 aa27531809-No
ORF function : Passenger Gene
Annotation : chromate resistance proteinDescription :

ORF sequence :

MSFLALFVSLPTKASTGRMRVWRSVKAQGCATLRDGVYLLPDSADSAATLGEVAAQAVEVGGSGQVYRLSGCDEAQKAALRALFDRGEEYASIAEEIKAL
GRNLASPDGADAMRKLQPLVRRFEQVNRIDFFPGEAQRQTLSLLDDLRDAITRRMSPDEPTARQTDIPRLNRADYQGRTWATRARPWVDRLASAWLIRRF
IDPSARIVWLASPSDCRNGWLGFDFDGAAFSHVGTKVTFETLLASFGLDSAPALVRLGKLVHCLDLGGLPVAEALGIESLLAGLRASEPDDDALLARACE
IFDWLLKSYEDKTT

 

Blast result :
ORF 4
LengthBeginEndStrandFusion ORF
561 bp186 aa30293589+No
ORF function : Accessory Gene
AG : Tn3 resolvase

ORF sequence :

MQGQRIGYVRVSSYDQNPERQLEQVEIGKLFTDKASGKDTQRPQLEAMLGFVREGDTVVVHSMDRLARNLDDLRRLVQRLTKRGVRIEFLKEGLVFTGED
SPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDDQAITLRQRAAAGEPKAQLAREFGISRETLYQYLRTDD

 

Blast result :
ORF 5
LengthBeginEndStrandFusion ORF
2967 bp988 aa35936559+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPRRSILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGDGNRLGFAVQLCLLRYPGYALGNDTALPEPVIQWVAKQVQADPASWAKYGERDVTR
REHALELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPTLSVIDRACSEAIARANRRVYRALIEPLNPSHHAKLDELLTIK
AGSNSTWLTWLRQSPLKPNSRHMIEHIERLKIFQLVGLPDSLGRHIHQNRLLKLAREGGQMTPQDLGKFEPERRYATLVAVVLESTATVIDELVDLHDRI
LVKLFSGAKHKHQQQFQKQGKAINDKVRLYSKIGQALLDAKESGDDPFAAIEAVIPWDEFTQSVTEAELLARPEAFDHLHLVSENFATLRRYTPAFLEVL
QLRAATAAQAVLDAVQTLREMNADNLRKVPSDAPTAFIKPRWKPLVITPEGIDRRFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPPEKFAALKR
EQALPLAINPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDSAVPNTAQALIDQTSQLLPRIKITELLMDVDEWTGFTRHFTHLKD
GAQAKDRTLLLTAILGDAINLGLTKMAESSPGMTYAKLSWLQAWHIRDETYSTALAELVNSQFRHAFAANWGDGTTSSSDGQRFRAGGKGESTGHVNPKY
GSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPNSVQDYPTLRPML
GGTLNIKHVCAHWDEILRLAASIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEI
RDRSFEQQRYRASGLNLVTAAIVLWNTVYLERATQAREEAGKPVNPELLQYLSPLGWEHINLTGDYVWRQSRKLEDGKFRPLRQLGKP

 

Blast result :
Comments
The transposase (ORF5) is 84% aa similar to ISPa38.
The others ORF : ORF1 : manganese and iron superoxide dismutase,
ORF2: chromate resistance protein,
ORF3: chromate resistance protein,
ORF4: resolvase domain-containing protein.

The partial transposase of ISPa58 is 97% aa similar to the transposase of ISPa40 (aa 200-988). ISPa58 is found in Pseudomonas aeruginosa (KC821786, JX194160).

File updated the February 13 2015.
References
1] ISfinder annotation (2011)
2] Martin Holm Rau (2011) Direct submission to ISfinder.